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Ursolic acid sensitized colon cancer cells to chemotherapy under hypoxia by inhibiting MDR1 through HIF-1α

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第9期17卷 672-682页

作者： Jian-zhen SHAN yan-yan xuan Qi ZHANG Jian-jin HUANG Department of Medical Oncology the Second Affiliated HospitalSchool of MedicineZhejiang University Cancer Institute of Zhejiang University Key Laboratory of Cancer Prevention and InterventionChina National Ministry of Education Department of General Surgery the Second Affiliated HospitalSchool of MedicineZhejiang University

Objective： To explore the efficacy of ursolic acid in sensitizing colon cancer cells to chemotherapy under hypoxia and its underlying mechanisms. Methods： Three colon cancer cell lines （RKO, LoVo, and SW480） were used as in vitro models. 5-Fluorouracil （5-FU） and oxaliplatin were used as chemotherapeutic drugs. Cell viability and apoptosis were tested to evaluate the sensitivity of colon cancer cells to chemotherapy. The transcription and ex- pression levels of hypoxia-inducible factor-1α （HIF-1α）, multidrug resistance gene 1 （MDR1）, and vascular endothelial growth factors （VEGF） were assessed by quantitative real-time polymerase chain reaction （qRT-PCR） and im- munoblotting. Cycloheximide and MG132 were used to inhibit protein synthesis and degradation, respectively. In vitro tube formation assay was used to evaluate angiogenesis. Results： We demonstrated the chemosensitizing effects of ursolic acid with 5-FU and oxaliplatin in three colon cancer cell lines under hypoxia. This effect was correlated to its inhibition of MDR1 through HIF-la. Moreover, ursolic acid was capable of inhibiting HIF-1α accumulation with little effects on its constitutional expression in normoxia. In addition, ursolic acid also down-regulated VEGF and inhibited tumor angiogenesis. Conclusions： Ursolic acid exerted chemosensitizing effects in colon cancer cells under hypoxia by inhibiting HIF-la accumulation and the subsequent expression of the MDR1 and VEGF.

关键词： Ursolic acid Colon cancer Hypoxia-inducible factor-1α (HIF-1α) Multidrug resistance gene 1 (MDR1) Drug resistance

劳动教育融入农业机械化及其自动化专业课程体系的探索与实践

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安徽农业科学 2024年第10期52卷 274-277页

作者：颜玄洲王玲娟李琴江西农业大学工学院江西南昌330045 江西农业大学农业机械化发展研究中心江西南昌330045 江西农业大学人文与公共管理学院江西南昌330045

劳动教育作为全面发展教育体系的一部分,具有独特的育人价值。将劳动教育融入课程体系是推进高校劳动教育顺利开展的途径。以农业机械化及其自动化专业为例,通过在课程体系中设置耕读劳动教育课程、在专业课程教学中渗透劳动教育、在专... 详细信息

劳动教育作为全面发展教育体系的一部分,具有独特的育人价值。将劳动教育融入课程体系是推进高校劳动教育顺利开展的途径。以农业机械化及其自动化专业为例,通过在课程体系中设置耕读劳动教育课程、在专业课程教学中渗透劳动教育、在专业实践教学中强化劳动教育3个方面开展劳动教育融入课程体系的探索与实践。研究表明,学生对劳动教育价值的认同普遍较高、耕读劳动教育课程能够迎合学生需求、劳动教育融入课程教学效果良好、劳动教育能够很好地融入实践教学。因此,通过深化学生对劳动教育的认知、完善耕读劳动教育课程设置、挖掘课程中的劳动教育资源,推进劳动教育更好地融入课程体系。

关键词：劳动教育课程体系农机专业

Ursolic acid inhibits proliferation and induces apoptosis of HT-29 colon cancer cells by inhibiting the EGFR/MAPK pathway

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第9期10卷 668-674页

作者： Jian-zhen SHAN yan-yan xuan Shu ZHENG Qi DONG Su-zhan ZHANG Department of Traditional Chinese Medicine the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Cancer Institute School of Medicine Zhejiang University Hangzhou 310009 China

Objective： To investigate the effects ofursolic acid on the proliferation and apoptosis of human HT-29 colon cancer cells. Methods： 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） and flow cytometry assays were performed to evaluate the effects of ursolic acid on the growth and apoptosis of HT-29 cells. Western blot analysis was applied to investigate the inhibitory efibcts of ursolic acid on the phosphorylation of the epidermal growth factor receptor （EGFR）, extracellular signal-regulated kinase （ERK）, c-Jun N-terminal kinase （JNK）, and p38 mitogen-activated protein kinase （p38 MAPK）, and the activity of B cell leukemia-2 （Bcl-2）, B cell leukemia-xL （Bcl-xL）, caspase-3, and caspase-9. Results： Ursolic acid inhibited the growth of HT-29 cells in dose- and time-dependent manners. The median inhibition concentration （IC50） values for 24, 48, and 72 h treatment were 26, 20, and 18 pmol/L, respectively. The apoptotic rates of 10, 20, and 40 μmol/L ursolic acid treatments for 24 h were 5.74%, 14.49%, and 33.05%, and for 48 h were 9%, 21.39%, and 40.49%, respectively. Ursolic acid suppressed the phosphorylation of EGFR, ERK1/2, p38 MAPK, and JNK, which is well correlated with its growth inhibitory effect. 10, 20, and 40 μmol/L ursolic acid significantly inhibited the proliferation of EGF-stimulated HT-29 cells （P〈0.05）. Cell proliferation was most significantly inhibited when treated with 10 and 20 μmol/L ursolic acid combined with 200 nmol/L AG 1478 or 10 μmol/L U0126 （P〈0.01）. Besides, it also down-regulated the expression of Bcl-2 and Bcl-xL and activated caspase-3 and caspase-9. Conclusion： Ursolic acid induces apoptosis in HT-29 cells by suppressing the EGFR/MAPK pathway, suggesting that it may be a potent agent for the treatment of colorectal cancer.

关键词： Colon cancer Ursolic acid Epidermal growth factor receptor (EGFR) Mitogen-activated protein kinase (MAPK) Apoptosis

Enhanced ozonation degradation of atrazine in the presence of nano-ZnO： Performance, kinetics and effects

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Journal of Environmental Sciences 2017年第11期29卷 3-13页

作者： Xiangjuan Yuan xuan yan Haiming Xu Dongya Li Lei Sun Gang Cao Dongsheng Xia School of Enuironmental Engineering Wuhan Textile University Wuhan 430073 China Engineering Research Center for Clean Production of Textile Dyeing and Printing Ministry of Education Wuhan 430073 China

Enhanced ozonation degradation of atrazine（ATZ） with nano-ZnO（nZnO） as catalyst and the influences of the operational parameters have been investigated through semi-continuous experiments in this study. The results demonstrated that the combination of ozone（O3） and nZnO showed an obvious synergetic effect and the ATZ degradation conformed to pseudo-first-order kinetics. An improvement of ATZ degradation efficiency by 41.8% and pseudo-first-order rate constant by more than a factor of four was obtained in the O3/nZnO process after 5 min of reaction compared to O3 alone. Meanwhile, the degradation efficiency of ATZ was gradually enhanced with increasing nZnO dosage and initial pH in the range from 3.0 to 8.0, and a higher amount of ATZ was degraded when the initial concentration of ATZ rose from 0.5 to 5 mg/L. Additionally, sulfate ion, chloride ion, nitrate ion and low concentrations of humic acid substances led to enhancement of the ATZ degradation. The notable decrease of ATZ removal efficiency observed in the presence of radical scavengers and the results of free radical tests indicated thatUOH is the dominant active radical species. The mechanism investigation demonstrated that the enhancement effect could be attributed to the introduction of nZnO,which could promote the utilization of O3, enhance the formation of superoxide radical, and further accelerate the production of hydrogen peroxide and the generation of OH/O2-.

关键词： Nano-ZnO Atrazine Catalytic ozonation Reaction mechanism

Effective removal of chlorinated organic pollutants by bimetallic iron-nickel sulfide activation of peroxydisulfate

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Chinese Chemical Letters 2020年第6期31卷 1535-1539页

作者： xuan yan Dongting Yue Chao Guo Songling Wang Xufang Qian Yixin Zhao School of Environmental Science and Engineering Shanghai Jiao Tong UniversityShanghai 200242China

Chlorinated organic pollutants(COPs)have caused serious contaminants in soil and groundwater,hence developing methods to remove these pollutants is necessary and *** a simple hydrothermal method,we synthesized the bimetallic iron-nickel sulfide(FeNiS)particles which exhibited excellent catalytic property of COPs *** was chosen as the peroxydisulfate(PDS)activator to removal COPs including 4-chlorophenol(4-CP),1,4-dichlorophenol(1,4-DCP)and 2,4,6-trichlorophenol(2,4,6-TCP).The results show that FeNiS can efficiently activate PDS to produce sulfate radical(SO4·-)which plays major role in the oxidative dechlorination and degradation due to its strong oxidizing property and the ability of producing hydroxyl radicals(·OH)in the alkaline ***,the Cl-abscised from COPs during the dechlorination can turn into the chlorine radicals and enhance the degradation and cause further mineralization of intermediate *** bimetallic FeNiS catalyst is a promising PDS activator for removal of chlorinated organics.

关键词： Peroxydisulfate oxidation Bimetallic iron-nickel sulfide Chlorinated organic pollutants Sulfate radicals Dechlorination

临床教学实践与探索:麻醉学专业紧缺人才培养

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麻醉安全与质控 2024年第2期6卷 101-104页

作者：亚力·亚森程虎宣燕邹田田新疆医科大学第一附属医院麻醉科新疆乌鲁木齐830000

作为新疆首批麻醉学专业紧缺人才培养基地,新疆医科大学第一附属医院麻醉科(以下简称新医大一附院麻醉科)的教学实践与探索不断向前推进。本文归纳总结麻醉专业紧缺人才培养的具体做法,探讨培养过程中暴露出来的问题。通过进一步完善教... 详细信息

作为新疆首批麻醉学专业紧缺人才培养基地,新疆医科大学第一附属医院麻醉科(以下简称新医大一附院麻醉科)的教学实践与探索不断向前推进。本文归纳总结麻醉专业紧缺人才培养的具体做法,探讨培养过程中暴露出来的问题。通过进一步完善教学体系,培养同质优秀人才,与提高麻醉学专业紧缺人才培养相呼应,促进麻醉学科建设与发展。

关键词：麻醉专业紧缺人才培养基地麻醉科

基于CiteSpace的低出生体重儿护理研究可视化分析

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中国当代医药 2024年第24期31卷 4-8,13页

作者：轩妍黄小君张燕美刘静路伟海南省妇女儿童医学中心护理部海南海口570000 海南医学院管理学院海南海口571199 汕头大学医学院附属粤北人民医院运营办广东韶关512026

目的通过检索中国期刊全文数据库(CNKI),梳理低出生体重儿护理研究文献,分析研究现状、发展以及热点前沿。方法应用CiteSpace对2000—2022年低出生体重儿护理相关研究进行可视化分析,检索式为“低出生体重儿”或“出生低体重儿”或“低... 详细信息

目的通过检索中国期刊全文数据库(CNKI),梳理低出生体重儿护理研究文献,分析研究现状、发展以及热点前沿。方法应用CiteSpace对2000—2022年低出生体重儿护理相关研究进行可视化分析,检索式为“低出生体重儿”或“出生低体重儿”或“低出生体重早产儿”或“早产低体重新生儿”,并且“护理”,主要研究文献发表数量与变化趋势,对作者合作、机构及关键词进行可视化分析。结果该研究领域受到研究者的普遍关注;2000—2022年检索低出生体重儿护理相关文献共869篇,作者合作团队内部联系紧密,但各团队之间缺少联系;发文机构团体合作不紧密;低出生体重儿护理研究关键词可分为11个聚类,聚类结果合理,各类别研究主题比较集中;探测突现术语20个,2010—2022年期间的热点词汇为“护理干预”“护理效果”和“延续性护理”等。结论应加强研究领域学者间的交流合作,加强低出生体重儿护理方式在临床上的灵活应用,充分结合联合护理的优势,提高我国新生儿健康水平。

关键词：低出生体重儿护理可视化 CiteSpace

A benchmark-based method for evaluating hyperparameter optimization techniques of neural networks for surface water quality prediction

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Frontiers of Environmental Science & Engineering 2024年第5期18卷 13-27页

作者： xuan Wang yan Dong Jing yang Zhipeng Liu Jinsuo Lu School of Environmental and Municipal Engineering Xi’an University of Architecture and TechnologyXi’an710055China Shaanxi Key Laboratory of Environmental Engineering Xi’an University of Architecture and TechnologyXi’an710055China State Key Laboratory of Green Building in West China Xi’an University of Architecture and TechnologyXi’an710055China

Neural networks(NNs)have been used extensively in surface water prediction tasks due to computing algorithm improvements and data *** essential step in developing an NN is the hyperparameter *** practice,it is common to manually determine hyperparameters in the studies of NNs in water resources *** may result in considerable randomness and require significant computation time;therefore,hyperparameter optimization(HPO)is *** study adopted five representatives of the HPO techniques in the surface water quality prediction tasks,including the grid sampling(GS),random search(RS),genetic algorithm(GA),Bayesian optimization(BO)based on the Gaussian process(GP),and the tree Parzen estimator(TPE).For the evaluation of these techniques,this study proposed a method:first,the optimal hyperparameter value sets achieved by GS were regarded as the benchmark;then,the other HPO techniques were evaluated and compared with the benchmark in convergence,optimization orientation,and consistency of the optimized *** results indicated that the TPE-based BO algorithm was recommended because it yielded stable convergence,reasonable optimization orientation,and the highest consistency rates with the benchmark *** optimization consistency rates via TPE for the hyperparameters hidden layers,hidden dimension,learning rate,and batch size were 86.7%,73.3%,73.3%,and 80.0%,*** the evaluation of HPO techniques directly based on the prediction performance of the optimized NN in a single HPO test,the proposed benchmark-based HPO evaluation approach is feasible and robust.

关键词： Neural networks Hyperparameter optimization Surface water quality prediction Bayes optimization Genetic algorithm

基于“互联网+”健康管理模式在口腔种植患者围手术期的应用

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中国口腔种植学杂志 2024年第1期29卷 56-61页

作者：宣岩郝俊燕安阳张婷张文静李丹北京大学口腔医院第二门诊部 100101

目的探讨基于“互联网+”的健康管理模式在口腔种植患者围手术期中的应用效果。方法选取北京大学口腔医院2023年1月至2023年10月进行口腔种植的患者58例,按照随机数字表法将其随机分为试验组29例和对照组29例。对照组实施常规健康管理模... 详细信息

目的探讨基于“互联网+”的健康管理模式在口腔种植患者围手术期中的应用效果。方法选取北京大学口腔医院2023年1月至2023年10月进行口腔种植的患者58例,按照随机数字表法将其随机分为试验组29例和对照组29例。对照组实施常规健康管理模式,试验组给予基于“互联网+”的健康管理模式,干预后比较两组患者种植手术术后并发症发生率、术前准备依从性、牙科畏惧情况及种植手术满意度的差异。结果试验组患者在术前牙周治疗和挂号时测量血压方面依从性高于对照组,牙科畏惧总分及其各维度方面的得分均低于对照组,对术后复诊提醒和医护服务态度满意度等方面得分高于对照组,差异均具有统计学意义(P0.05)。结论基于“互联网+”的健康管理模式有利于提高口腔种植患者术前牙周治疗和挂号时测量血压的依从性,减轻种植患者手术时的牙科畏惧,在术后复诊提醒方面具有明显的优势。

关键词：互联网+ 健康管理口腔种植围手术期