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Knockin of the G275E mutation of the nicotinic acetylcholine receptor (nAChR) α6 confers high levels of resistance to spinosyns in Spodoptera exigua

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Insect Science 2022年第2期29卷 478-486页

作者： Ya-Yun Zuo Yu-Xin Xue Ze-Yu Wang xuan ren Ahmed A.A.Aioub Yi-Dong Wu Yi-Hua Yang Zhao-Nong Hu Institute of Pesticide Science College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi712100 China College of Plant Protection Nanjing Agricultural UniversityNanjing210095 China Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi712100 China Key Laboratory for Botanical Pesticide R&D of Shaanxi Province YanglingShaanxi712100 China Plant Protection Department Faculty of AgricultureZagazig UniversityZagazig44511 Egypt

Spinosyns,including spinosad and spinetoram,act on the insect central nervous system,gradually paralyzing or destroying the target insect.Spinosad resistance is associated with loss-of-function mutations in the nicotinic acetylcholine receptor(nAChR)α6 subunit in a number of agricultural pests.Using gene editing,nAChRα6 has been verified as a target for spinosyns in five insect species.Recently,a point mutation(G275E)in exon 9 of nAChRα6 was identified in spinosad-resistant strains of Thrips palmi and Tuta absoluta.To date,no in vivo functional evidence has been obtained to support that this mutation is involved in spinosyn resistance in lepidopteran pests.In this study,the G275E mutation was introduced into the nAChR of Spodoptera exigua using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)gene-editing technology.Reverse transcriptase-polymerase chain reaction and sequencing confirmed that this mutation was present in exon 9 of the nAChR transcripts in the edited 275E strain.The results of bioassays showed that the 275E strain was highly resistant to spinosad(230-fold)and spinetoram(792-fold)compared to the unedited background strain,directly confirming that the G275E mutation of the nAChRα6 subunit confers high levels of spinosyn resistance in S.exigua.Inheritance analysis showed that the resistance trait is autosomal and incompletely recessive.This study employs a reverse genetics approach to validate the functional role played by the G275E mutation in nAChRα6 of S.exigua in spinosyns resistance and provides another example of the use of CRISPR/Cas9 gene-editing technology to confirm the role played by candidate target site mutations in insecticide resistance.

关键词： beet armyworm CRISPR G275E mutation nicotinic acetylcholine recep・tor spinosyns insecticides target resistance

Effects of Spatial Density of Farmland Shelterbelts on NDVI on the Northern Slope of Tianshan Mountains

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Asian Agricultural Research 2022年第10期14卷 29-32页

作者： Li CHEN Jie GAO Mengdi CHANG xuan ren Xinjiang Academy of Environmental Protection Science Urumqi 830011China Xinjiang University Urumqi Xinjiang 830046 Xinjiang Key Laboratory for Environmental Pollution Monitoring and Risk Warning Urumqi 830011China Xinjiang Engineering Technology Research Center for Cleaner Production Urumqi 830011China Junggar Desert-oasis Ecotone Station for Scientific Observation and Research of National Environmental Protection Urumqi 830011China

[Objectives]To explore the effects of spatial density of farmland shelterbelts on NDVI on the northern slope of Tianshan Mountains.[Methods]Taking the economic belt of the northern slope of the Tianshan Mountains as the research area and using the grid method,the spatial density distribution of farmland shelterbelts can be known.Combining the grid method with NDVI data,the average value of normalized difference vegetation index(NDVI)during the growing period of crops can be obtained.In addition,the protection benefits of the shelterbelts on crops were analyzed through comparing the growth of crops within the protection zone with and without protection.[Results]The grids with shelterbelt distribution were better than the grids without shelterbelt distribution,and the shelterbelt played a great role in promoting crop growth.In the middle stage of crop growth,the protection benefit of shelterbelt was significant.The spatial density of shelterbelts was unevenly distributed in the range of 0.6 to 0.8,and the protection benefits were poor.[Conclusions]It is expected to explore the effects of shelterbelts on crop growth at a larger regional scale,so as to provide a basis for the management and design of shelterbelts in the future,and to provide a theoretical basis for studying the protective effect of farmland shelterbelts on crops.

关键词： Farmland Shelterbelts Spatial density Normalized difference vegetation index (NDVI)

Robust autofocusing propagation in turbulence

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Chinese Physics B 2024年第6期33卷 370-375页

作者：刘娜娜谭柳陈凯健洪佩龙莫小明邹炳锁任煜轩梁毅 Guangxi Key Laboratory for Relativistic Astrophysics Center on Nanoenergy ResearchSchool of Physical Science and TechnologyGuangxi UniversityNanning 530004China School of Mathematics and Physics Anqing Normal UniversityAnqing 246133China Institute for Translational Brain Research MOE Frontiers Center for Brain ScienceFudan UniversityShanghai 200032China

Turbulence in complex environments such as the atmosphere and biological media has always been a great challenge to the application of beam propagation in optical communication, optical trapping and manipulation. To overcome this challenge, this study comprehensively investigates the robust propagation of traditional Gaussian and autofocusing beams in turbulent environments. In order to select stable beams that exhibit high intensity and high field gradient at the focal position in complex environments, Kolmogorov turbulence theory is used to simulate the propagation of beams in atmospheric turbulence based on the multi-phase screen method. We systematically analyze the intensity fluctuations, the variation of the coherence factor and the change in the scintillation index with propagation distance. The analysis reveals that the intensity fluctuations of autofocusing beams are significantly smaller than those of Gaussian beams, and the coherence of autofocusing beams is better than that of Gaussian beams under turbulence. Moreover, autofocusing beams exhibit less oscillation than Gaussian beams, indicating that autofocusing beams propagate in complex environments with less distortion and intensity fluctuation. Overall, this work clearly demonstrates that autofocusing beams exhibit higher stability in propagation compared with Gaussian beams, showing great promise for applications such as optical trapping and manipulation in complex environments.

关键词： propagation beams autofocusing turbulence

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某型变量泵压力超高故障分析与改进

液压气动与密封 2024年第2期44卷 106-110页

作者：刘雨陈宣任杨斌中航力源液压股份有限公司贵州贵阳550018 空装成都局驻贵阳地区第三军事代表室贵州贵阳550018

针对某型变量泵壳体回油压力超高问题,运用AMESim软件对变量泵进行仿真分析,得出了转子倾覆和控制系统响应过快是导致压力超高的主要原因,最后,根据仿真结果对相关结构进行优化设计,并试验验证了改进措施的有效性。

关键词：变量泵压力超调 AMESim

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零价钨活化高碘酸盐降解水中双酚A的研究

四川环境 2024年第2期43卷 159-166页

作者：侯耀杨莹熊仁萱西安工程大学环境与化学工程学院西安710600 西南交通大学地球科学与环境工程学院成都610031

双酚A(BPA)作为一种具有代表性的环境内分泌干扰物质,每年产量巨大,但即使以痕量水平进入水体,也可通过生物积累对人体造成危害,故减少双酚A造成的污染迫在眉睫。采用高级氧化技术降解BPA,零价钨(W^(0))作为催化剂活化高碘酸盐(PI),建立... 详细信息

双酚A(BPA)作为一种具有代表性的环境内分泌干扰物质,每年产量巨大,但即使以痕量水平进入水体,也可通过生物积累对人体造成危害,故减少双酚A造成的污染迫在眉睫。采用高级氧化技术降解BPA,零价钨(W^(0))作为催化剂活化高碘酸盐(PI),建立W^(0)/PI高效催化氧化体系,探究体系降解BPA的影响因素,明确其催化剂稳定性以及反应机制。结果表明,体系温度为25±1℃,PI浓度为1.0 mM、W0浓度为0.3 g/L、BPA浓度为20μM,反应60 min后BPA去除率可达97.66%,W0稳定性较好,该体系中超氧自由基(O_(2)^(·-))和单线态氧(^(1)O_(2))是主要活性物种,且溶解氧对W^(0)/PI体系降解BPA起到促进作用。由此,丰富了金属催化剂活化高碘酸盐催化剂种类,为W^(0)/PI体系在环境中难降解污染物治理中的应用提供了一定的理论依据和数据支撑,为实际工业废水处理应用提供了理论支撑。

关键词：高级氧化高碘酸盐零价钨双酚A

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飞秒激光辅助下超声乳化术、普通白内障超声乳化术对年龄相关性白内障患者眼内压及最佳矫正视力、视盘区血流密度的影响

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中国当代医药 2024年第17期31卷 83-86页

作者：任旋赖梦云谌婷婷南昌爱尔眼科医院眼底科江西南昌330000 南昌爱尔眼科医院护理部江西南昌330000

目的比较飞秒激光辅助下超声乳化术(FLACS)、普通白内障超声乳化术(CPCS)在年龄相关性白内障(ARC)患者中的效果。方法选取2021年7月至2023年7月南昌爱尔眼科医院收治的70例ARC患者作为研究对象,按随机数字表法分为对照组(35例)、观察组... 详细信息

目的比较飞秒激光辅助下超声乳化术(FLACS)、普通白内障超声乳化术(CPCS)在年龄相关性白内障(ARC)患者中的效果。方法选取2021年7月至2023年7月南昌爱尔眼科医院收治的70例ARC患者作为研究对象,按随机数字表法分为对照组(35例)、观察组(35例)。对照组行CPCS,观察组行FLACS,两组术后均随访3个月。比较两组的手术相关指标、眼内压、黄斑中心凹厚度(CMT)、最佳矫正视力(BCVA)、视盘区血流密度、并发症等。结果观察组的超声乳化时间为(14.57±2.30)min,短于对照组的(22.49±3.58)min,观察组的超声乳化能量为(5.39±1.20)ml,低于对照组的(11.33±2.15)ml,观察组的并发症总发生率为5.71%(2/35),低于对照组的22.86%(8/35),差异有统计学意义(P0.05);两组患者术后1 d,术后1周,术后1、3个月的BCVA均高于本组术前,差异有统计学意义(P0.05)。结论FLACS具有更短的超声乳化时间,更少的乳化能量,其可提升视力,且不会加重对眼压、视盘区血流密度与CMT的影响,并发症较少。

关键词：年龄相关性白内障普通白内障超声乳化术飞秒激光并发症

考虑荷电状态的光伏微电网混合储能容量优化配置

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电测与仪表 2024年第2期61卷 150-156页

作者：任萱李桐歌马骏毅黄永红张大林吴玉洁国网镇江供电公司江苏镇江212000 江苏大学电气信息工程学院江苏镇江212013

储能系统容量优化配置是提高系统稳定性、降低微电网成本的有效措施之一。文中提出了一种考虑荷电状态的能量管理策略,对光伏微电网混合储能系统进行容量配置。综合分析微电网运行的稳定性和经济效益,以全生命周期费用和买卖电量费用之... 详细信息

储能系统容量优化配置是提高系统稳定性、降低微电网成本的有效措施之一。文中提出了一种考虑荷电状态的能量管理策略,对光伏微电网混合储能系统进行容量配置。综合分析微电网运行的稳定性和经济效益,以全生命周期费用和买卖电量费用之和最小为目标,建立含超级电容和蓄电池的光伏微电网储能模型,结合光伏可供能量和负荷需求功率,应用改进能量管理策略和粒子群算法,建立光伏微电网混合储能系统(HESS)容量配置双层优化模型。以某地实际数据为例对优化问题进行求解,将优化结果与传统储能配置方法进行对比,验证了所提方法的有效性,为光伏微电网混合储能系统容量优化配比提供参考。

关键词：混合储能系统荷电状态能量管理策略粒子群算法双层优化

Enantioselective Synthesis of Benzimidazole Atropisomers Featuring a N-N Axis

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Chinese Journal of Chemistry 2024年第7期42卷 711-718页

作者： Fang-Bei Ge Qi-Kun Yin Chuan-Jun Lu xuan xuan Jia Feng ren-Rong Liu College of Chemistry and Chemical Engineering Qingdao UniversityQingdaoShandong 266071China School of Pharmacy Key Laboratory of Mlecular Pharmacoogy and Drug EvaluationMinistry of EducationYantai Universit YantaiShandong 264005China

Atroposelective synthesis of N-N atropisomers is an emerging area but remains underexplored;in particular,the synthesis of N-N benzimidazole atropisomers is still unprecedented.Herein,the first enantioselective synthesis of N-N benzimidazole atropisomers via the palladium-catalyzed de novo construction of benzimidazole skeleton is presented.With readily available palladium catalyst and biphosphine ligand,a broad range of nonbiaryl benzimidazole and indole-benzimidazole atropisomers were conveniently ac-cessed in high yields and with excellent enantioselectivities.Significantly,these N-N benzimidazole atropisomers showed great anti-tumor activity and selectivity to breast cancer MCF-7 cells.The simple catalytic system,broad substrate scope,high enantioselectivi-ty,and good bioactivity make this approach highly attractive.

关键词： Asymmetric synthesis Atropisomerism Palladium Benzimidazole Antitumor

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基于纵横交叉算法的储能系统自适应控制策略

电气传动 2024年第4期54卷 4-10,20页

作者：任萱陈通董立志黄灵通张明霞国网江苏省电力有限公司镇江供电分公司江苏镇江212000 中国电力科学研究院有限公司北京100192

大量储能设备通过虚拟同步机(VSG)并入电网时,传统固定惯量和阻尼控制策略中,控制参数选取不当会造成调节时间长或超调量大,且无法充分发挥VSG控制灵活的优势。为此,提出了基于纵横交叉(CSO)算法的储能系统自适应控制策略,首先,建立了... 详细信息

大量储能设备通过虚拟同步机(VSG)并入电网时,传统固定惯量和阻尼控制策略中,控制参数选取不当会造成调节时间长或超调量大,且无法充分发挥VSG控制灵活的优势。为此,提出了基于纵横交叉(CSO)算法的储能系统自适应控制策略,首先,建立了储能系统VSG系统,将储能VSG系统频率的误差与电压总谐波畸变率之和的最小值作为CSO的目标函数,并引入电池的荷电状态(SOE)约束求解最佳惯量以及阻尼,该算法收敛速度更快且有效地规避了参数局部解。在此基础上,设计一种改进的惯量阻尼自适应控制策略,有效地改善了VSG的动态性能。最后,通过Matlab/Simulink搭建仿真模型,验证了所提策略的有效性。

关键词：储能系统虚拟同步机惯量阻尼自适应控制电池荷电状态纵横交叉算法

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