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Fire needling and bloodletting at cleft points in treatment of 35 patients with acute gouty arthritis

World Journal of Acupuncture-Moxibustion 2016年第2期26卷 73-78页

作者：许素瑜涂云王志花湛江中心人民医院中医科广东湛江524000

Objective To observe the clinical efficacy of fire needling and bloodletting at cleft points for acute gouty arthritis, and to explore its functional mechanism. Methods Thirty-five patients with acute gouty arthritis were enrolled into this study, and fire needling and bloodletting with 10 mL/ time were applied at cleft points of corresponding meridians and collaterals at the affected side. The treatment was conducted for once every other day, and treatment for three consecutive times was needed. Serum uric acid （UA） and pain score were tested in patients before treatment and on the 6th day after treatment, follow-up visit for 3 months was performed in patients who stopped treatment, and recurrence rate was calculated. Results Budzyuski 6-point behavioral rating scale was applied to score pain. T-test was conducted on mean and standard deviation of pain score before treatment （4.09 ＋ 0.82） and after treatment （1.14 ＋ 1.33）, showing that the difference was significant （P〈0.05）; t-test was also conducted on mean and standard deviation of serum UA before treatment [（555.34 ＋ 53.09） pmol/L] and after treatment [（414.23 ＋ 67.04） pmol/L], showing that the difference was significant （P〈0.05）; among the 35 patients with acute gouty arthritis, 14 patients were cured （40.0%）, improvement was found in 19 patients （54.3%）, and effectiveness was found in 33 patients （94.3%）. Based on follow-up visit for 3 months in 33 patients with efficacy, recurrence was found in 3 patients （9.1%）. Conclusion Fire needling and bloodletting at cleft points is an effective method in treatment of acute gouty arthritis with significant analgesic effect, efficacy of reducing serum UA, high cure rate and low recurrence rate, which is worth of being generalized clinically.

关键词： acute gouty arthritis cleft point fire needling bloodletting

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生物质缓释土壤保水增肥剂对宁夏盐池县土壤及其作物的影响

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中国土壤与肥料 2024年第7期 129-134页

作者：武金龙李郝琪马锋茂韩凤兰黄家和刘燕华邵自强江成云北方民族大学材料科学与工程学院宁夏银川750021 北方民族大学高分子材料及制造技术重点实验室宁夏银川750021 “工业废弃物循环利用及先进材料”国际科技合作基地宁夏银川750021 宁夏盐池县农牧科学研究所宁夏盐池751500 北京理工大学材料学院北京100081 宁夏宁东市政建设发展有限公司宁夏银川750409

为探讨生物质缓释土壤保水增肥剂对农田土壤水温环境、土壤养分吸收和作物产量的影响,在宁夏回族自治区吴忠市盐池县以玉米、马铃薯、燕麦等为供试作物,生物质缓释土壤保水增肥剂为供试材料,采用田间试验,设置生物质缓释土壤保水增肥剂... 详细信息

为探讨生物质缓释土壤保水增肥剂对农田土壤水温环境、土壤养分吸收和作物产量的影响,在宁夏回族自治区吴忠市盐池县以玉米、马铃薯、燕麦等为供试作物,生物质缓释土壤保水增肥剂为供试材料,采用田间试验,设置生物质缓释土壤保水增肥剂不同梯度的施用量,测定土壤含水量、酸碱度、电导率、容重和土壤养分等,研究其对试验地区土壤理化性质、土壤养分以及不同作物产量的影响。结果表明:在施用量不同的情况下,所种植的作物总体上产量都有所增加。苏丹草和燕麦草增产率最高,分别达到36.26%和24.35%。荞麦在生物质缓释土壤保水增肥剂施用量为120kg/hm^(2)时产量增产率达到23.19%,而马铃薯在生物质缓释土壤保水增肥剂施用量为120kg/hm^(2)时产量增产率最高,达到了77.78%,增产效果显著。但玉米产量变化趋势有所不同,在生物质缓释土壤保水增肥剂施用量为60kg/hm^(2)时产量反而有所下降,当施用量增加至120kg/hm^(2)时产量有所上升,但增产率只有0.69%,主要是由于地块所处位置、地势以及土壤组分存在一定程度的差异性,导致生物质缓释土壤保水增肥剂施用量对玉米产量影响不大。

关键词：羧甲基纤维素农作物产量土壤性质保水增肥作用

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Effects of different molecular weights of chitosan on methane production and bacterial community structure in vitro

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Journal of Integrative Agriculture 2020年第6期19卷 1644-1655页

作者： TONG Jin-jin ZHANG hua WANG Jia LIU yun MAO Sheng-yong XIONG Ben-hai JIANG Linshu Beijing Key Laboratory for Dairy Cow Nutrition Beijing University of AgricultureBeijing 102206P.R.China Beijing Bei Nong Enterprise Management Co. Ltd.Beijing 102206P.R.China College of Veterinary Medicine Northeast Agricultural UniversityHarbin 150030P.R.China College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095P.R.China State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China

As a new feed additive,chitosan has been shown in recent years to have a certain role in reducing methane emissions from the gastrointestinal tracts of ***,the effects of chitosan with different molecular weights on rumen ferme ntation,methane production and bacterial comm unity structure are not yet clear.A basal diet without chitosa n served as the control(CTL),and the treatment diets were supplemented with chitosan with different molecular weights:1000(1K),3000(3K),5000(5K),50000(5W)and 200000(20W)dry matter(DM).Six fermentation units per treatment were *** chromatography was used to measure the co nee ntrations of metha ne,H2 and volatile fatty acids(VFAs).The bacterial 16S rRNA genes were sequeneed with an Illumina MiSeq platform and analysed to reveal the relative abunda nces of bacterial community *** results showed that the propi onate proporti on was sign ifica ntly in creased by the addition of chitosan with different molecular weights(P<0.05),while methane production and the acetate proportion were significantly decreased(P<0.05).The relative mbundances of Rikenellaceae_RC9_gut_group and Prevotellaceae_UCG_003 were significa ntly in creased in the 3K chitosa n group compared with the CTL group,whereas the relative abunda nee of Ruminococcaceae_NK4A214_group was significantly decreased(P<0.05).Correlation analyses of the relative abundances of the bacterial genera showed that Prevotella was positively related to propionmte production(Pv0.05).In conclusion,3K chitosan could reduce methane production by replacing fibrolytic bacteria(Firmicutes and Fibrobacteres)with amylolytic bacteria(Bacteroidetes and Proteobacteria)in the bacterial community structure.

关键词： chitosan high-throughput DNA sequencing methane

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Possible PKHD1 Hot-spot Mutations Related to Early Kidney Function Failure or Hepatofibrosis in Chinese Children with ARPKD:A Retrospective Single Center Cohort Study and Literature Review

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Current Medical Science 2020年第5期40卷 835-844页

作者： Li-ru QIU Rong-rong xu Jin-hui TANG Jian-hua ZHOU Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

PKHD1 mutations are generally considered to cause autosomal recessive polycystic kidney disease(ARPKD).ARPKD is a rare disorder and one o f the most severe conditions leading to end-stage renal disease in *** the biallelic deletion mutation,patients have difficulty in surviving the perinatal period,resulting in perinatal or neonatal *** study retrospectively analyzed patient characteristics,imaging characteristics,laboratory examinations and family surveys from 7 Chinese children with different PKHD1 gene mutations diagnosed by high-throughput sequencing from January 2014 to February 2018.O f the 7 children,there were 3 males and 4 *** missense mutations,two frameshift mutations,two deletion mutations,and two intronic slicing mutations were *** of the mutations have not previously been *** the literature search,we identified a total of 29 Chinese children with PKHD1 *** missense mutation c.2507T>C in exon 24 was found in one patient in our study,and five patients with liver fibrosis but normal renal function were reported in the *** missense mutation c.5935G>A in exon 37 was found in two patients in our study and three cases in the *** patients had renal failure at an age as young as 1 year of those five patients with the missense mutation c.5935G>A in exon *** was concluded that:(1)Kidney length more than 2-3 SDs above the mean and early-onset hypertension might be associated with PKHDI-associated ARPICD;(2)The more enlarged the kidney size is,the lower the renal function is likely to be;(3)c.5935G>A may be a hot spot that leads to early renal failure in Chinese children with PKHD1 mutations;(4)c.2507T>C may be a hot-spot mutation associated with hepatic lesions in Chinese children with PKHD1.

关键词： PKHD1 mutations autosomal recessive polycystic kidney disease clinical phenotype genotypic characteristics Chinese children

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宋银枝基于木郁土壅辨治更年期高血压的思路探讨

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广州中医药大学学报 2024年第9期41卷 2462-2466页

作者：陶洁李亮许禄华宋银枝(指导) 广州中医药大学第七临床学院深圳市宝安区中医院广东深圳518100

肝木与脾土互资互用,肝主疏泄、脾主运化协同配合是人体维持生命和机能的枢纽。若肝脾功能失调,肝失疏泄,脾失健运,出现木郁土壅的病理状态,则可影响肠道菌群的稳定性及神经系统的功能,出现脑-肠轴机制异常,从而导致更年期高血压的发生... 详细信息

肝木与脾土互资互用,肝主疏泄、脾主运化协同配合是人体维持生命和机能的枢纽。若肝脾功能失调,肝失疏泄,脾失健运,出现木郁土壅的病理状态,则可影响肠道菌群的稳定性及神经系统的功能,出现脑-肠轴机制异常,从而导致更年期高血压的发生与发展。宋银枝教授基于多年临证体会,提出木郁土壅可致更年期高血压,并结合现代研究分析其病理机制,探讨采用疏肝运脾法防治更年期高血压的思路。疏肝运脾之具体措施包括“健运脾土,祛痰化浊”“疏肝解郁,补益气血”“未病先防,调摄心神”,方药可选六君子汤、半夏白术天麻汤、逍遥散等。通过疏肝运脾,可使肝气调达,情志舒畅;脾肠健运,肠道菌群保持稳态平衡,膏脂代谢正常,使血压波动在正常范围。治疗更年期高血压可从肝脾入手,疏肝运脾是调治更年期高血压的切入点和关键所在。

关键词：更年期高血压木郁土壅脑-肠轴疏肝运脾六君子汤半夏白术天麻汤逍遥散宋银枝

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老年患者术前血清β_(2)微球蛋白浓度与术后谵妄的关系

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中华麻醉学杂志 2024年第2期44卷 145-149页

作者：王元龙贺倩化树慧徐钐凌孔荐巩红岩董瑞林亚男李传毕燕琳王彬林旭滨州医学院第二临床医学院烟台264003 青岛市市立医院麻醉科青岛266071

目的评价老年患者术前血清β_(2)微球蛋白(β_(2)MG)浓度与术后谵妄(POD)的关系。方法基于围术期神经认知障碍和生物标志物生活方式队列,属于巢式病例对照研究,选取2021年5月至2022年11月在青岛市市立医院择期于脊椎-硬膜外麻醉下行膝... 详细信息

目的评价老年患者术前血清β_(2)微球蛋白(β_(2)MG)浓度与术后谵妄(POD)的关系。方法基于围术期神经认知障碍和生物标志物生活方式队列,属于巢式病例对照研究,选取2021年5月至2022年11月在青岛市市立医院择期于脊椎-硬膜外麻醉下行膝或髋关节置换术患者,根据是否发生POD将患者分为POD组和非POD组。收集患者病例资料,分析组间差异;采用logistic回归筛选POD的危险因素;敏感性分析检验回归模型的稳定性;中介效应模型评估脑脊液(CSF)生物标志物在β_(2)MG对POD影响的中介效应;绘制受试者工作特征曲线并计算曲线下面积评价术前β_(2)MG浓度和CSF生物标志物浓度预测POD发生的准确性。结果POD组57例,非POD组449例。logistic回归分析在校正了年龄、性别、受教育程度、简易智力状态检查量表评分、高血压史、输液量等多个混杂因素后的结果表明:血清β_(2)MG和CSF总tau蛋白(t-tau)浓度升高为POD发生的危险因素,CSFβ淀粉样蛋白42浓度升高为POD发生的保护因素(P<0.05);中介效应分析结果显示,血清β_(2)MG浓度升高对POD的影响由t-tau部分介导;血清β_(2)MG浓度和CSF生物标志物浓度曲线下面积为0.742。结论老年患者术前血清β_(2)MG浓度升高为POD的危险因素,可能由CSF t-tau部分介导。

关键词：老年人 β_(2)微球蛋白谵妄手术后并发症

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黑豆与黄酒共制女贞子炮制工艺优化

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中成药 2024年第1期46卷 54-59页

作者：蒋云秀东宝花敖明月胡麟胡昌江陈志敏余凌英成都中医药大学药学院西南特色中药资源国家重点实验室四川成都611137

目的优化黑豆与黄酒共制女贞子炮制工艺。方法在单因素试验基础上,以蒸制时间、蒸制次数、烘制温度为影响因素,外观性状,红景天苷、特女贞苷、女贞苷G13、醇溶性浸出物含量,DPPH、ABTS自由基清除率的总评“归一值”(OD值)为评价指标,星... 详细信息

目的优化黑豆与黄酒共制女贞子炮制工艺。方法在单因素试验基础上,以蒸制时间、蒸制次数、烘制温度为影响因素,外观性状,红景天苷、特女贞苷、女贞苷G13、醇溶性浸出物含量,DPPH、ABTS自由基清除率的总评“归一值”(OD值)为评价指标,星点设计-响应面法优化炮制工艺。结果最优工艺为蒸制时间70 min,蒸制次数7次,烘制温度90℃,OD值为0.8415。结论该方法简便可靠,稳定可行,可用于黑豆黄酒共制女贞子。

关键词：女贞子黑豆黄酒炮制工艺星点设计-响应面法

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National Nutrition and Health Systematic Survey for Children 0–17 Years of Age in China

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Biomedical and Environmental Sciences 2021年第11期34卷 891-899页

作者： YANG Zhen Yu ZHANG Qian ZHAI Yi xu Tao WANG Yu Ying CHEN Bo Wen TANG xue Jun YUAN Xiao Lin FANG Hong yun ZHU Yan PANG xue Hong WANG Shuo xu Juan LI Rui Li SI Xiang ZHAO Wen hua National Institute for Nutrition and Health Chinese Center for Disease Control and PreventionBeijing 100050China Chinese Center for Disease Control and Prevention Beijing 102206China National Center for Women and Children's Health Chinese Center for Disease Control and PreventionBeijing 100081China Capital Institute of Pediatrics Beijing 100020China National Center for Statistics and Information National Health CommissionBeijing 100044China

The main purpose of the National Nutrition and Health Systematic Survey for children 0-17 years of age in China(CNHSC)was to collect basic data on the nutrition,development,and health status for children in different regions across China using evidence-based,reliable,and cost-effective *** and their parents or guardians from seven regions(south,southwest,north,northwest,eastern,central,and northeast China)in China were recruited.A multi-stage stratified randomized sampling method was *** provinces were randomly sampled from each of the seven regions,from which one urban district and one rural country were also randomly sampled,resulting in a total of 28 survey counties/*** surveys,health examinations,laboratory testing,and questionnaires were used to collect dietary intake,nutritional status,child development,and health status ***,health,and lifestyle assessment of children and their parents was determined using the Knowledge Attitude Practice(KAP)*** than 100,000 children(38,000 children<6 years of age and 66,000 children6-17 years of age)completed the *** survey provided comprehensive data on child nutrition and health status for future studies and will serve as the basis for an integrated nutrition and health improvement strategies proposal for children in China.

关键词： Children 0-17 years of age Nutritional status Health status Study protocol

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Chromosome-level genome assembly of Cylas formicarius provides insights into its adaptation and invasion mechanisms

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Journal of Integrative Agriculture 2023年第3期22卷 825-843页

作者： hua Jin-feng ZHANG Lei HAN Yong-hua GOU Xiao-wan CHEN Tian-yuan huaNG Yong-mei LI Yan-qing MA Dai-fu LI Zong-yun Institute of Integrative Plant Biology/Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics/School of Life Sciences Jiangsu Normal UniversityXuzhou 221116P.R.China Sweet Potato Laboratory Institute of Maize ResearchGuangxi Academy of Agricultural SciencesNanning 530007P.R.China xuzhou Academy of Agricultural Sciences/Institute of Sweet Potato Research Chinese Academy of Agricultural SciencesXuzhou 221131P.R.China

Cylasformicarius is one of the most important pests of sweet potato worldwide, causing considerable ecological and economic *** study improved the effect of comprehensive management and understanding of genetic mechanisms by examining the functional genomics of C. *** Illumina and PacBio sequencing, this study obtained a chromosome-level genome assembly of adult weevils from lines inbred for 15 *** high-quality assembly obtained was 338.84 Mb, with contig and scaffold N50 values of 14.97 and 34.23 Mb, *** total, 157.51 Mb of repeat sequences and 11 907 protein-coding genes were predicted.A total of 337.06 Mb of genomic sequences was located on the 11 chromosomes, accounting for 99.03%of the total length of the associated *** genomic analysis showed that C. formicarius was sister to Dendroctonus ponderosae, and C. formicarius diverged from D. ponderosae approximately 138.89 million years ago (Mya).Many important gene families expanded in the C. formicarius genome were involved in the detoxification of pesticides, tolerance to cold stress and chemosensory *** further study the role of odorant-binding proteins (OBPs) in olfactory recognition of C. formicarius, the binding assay results indicated that Cfor OBP4–6 had strong binding affinities for sex pheromones and other *** high-quality C. formicarius genome provides a valuable resource to reveal the molecular ecological basis, genetic mechanism, and evolutionary process of major agricultural pests;it also offers new ideas and new technologies for ecologically sustainable pest control.

关键词： Cylas formicarius PacBio sequencing high-through chromosome conformation capture chromosome-level genome chemosensory genes fluorescence competitive binding

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基层医务人员突发公共卫生事件韧性评价指标体系的构建

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中国健康教育 2024年第6期40卷 531-537,574页

作者：吴巧慧许军吴霖健刘莉刘琛方浩庭张金华南方医科大学公共卫生学院广东广州510515 南方医科大学南方医院广东广州510515 广东石油化工学院广东茂名525000

目的筛选并构建科学有效的基层医务人员突发公共卫生事件韧性评价体系。方法通过文献阅读法确定备选的指标,采用Delphi专家咨询法对备选指标进行3轮专家意见的征询,然后对筛选后的指标进行第4轮专家征询,最后采用属性层次分析法对韧性... 详细信息

目的筛选并构建科学有效的基层医务人员突发公共卫生事件韧性评价体系。方法通过文献阅读法确定备选的指标,采用Delphi专家咨询法对备选指标进行3轮专家意见的征询,然后对筛选后的指标进行第4轮专家征询,最后采用属性层次分析法对韧性评价指标体系赋予权重。共有25名专家参与Delphi法专家咨询。结果本研究所邀请的专家涉足近8个学科领域,平均专业年限15.69。前3轮征询专家一致性系数Kendall's W分别为0.372,0.409和0.394。建立了3个层级的指标体系:一级指标为基层医务人员的职业素养(权重0.346)、心理资本(权重0.614)和应急态度(权重0.040),二级指标有13个内容,进一步细分为46个三级指标。结论Delphi法结合属性层次分析法用于基层医务人员突发公共卫生事件韧性评估指标体系的筛选是有效可行的。构建的基层医务人员韧性评估体系能系统地评估基层医务人员面对突发公共卫生事件时所具备的韧性,可为提高基层医疗单位的突发公共卫生事件应对能力提供策略依据。

关键词：基层医务人员韧性公共卫生事件 Delphi法属性层次分析法

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