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Suppression of bcl-2 Gene by RNA Interference Increases Chemosensitivity to Cisplatin in Nasopharyngeal Carcinoma Cell Line CNE1

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 749-753页

作者： Zhi-Hua YIN, Cai-Ping REN, Feng LI, xu-Yu YANG, Hui LI, ming ZHAO, and Kai-Tai YAOCancer Research Institute, Central South University, Changsha 410078, China Cancer Research Institute Central South University

To explore the effect of suppressing BCL-2 expression using RNA interference (RNAi) technique in nasopharyngeal carcinoma cell line CNE1. CNE1 cell lines stably expressing shRNAs targeted bcl-2 and GL3 gene were established and gene expression inhibition was assessed by Western blotting analysis. The effect of suppressing bcl-2 by RNAi on cell growth was studied, the apoptosis induction and the sensitization of CNE1 cells to cisplatin were quantified by MTT assay and flow cytometry. The results showed that: stable transfection of CNE1

关键词： RNA interference nasopharyngeal carcinoma bcl-2 cisplatin

Spatial Prediction of Heavy Metal Pollution for Soils in Peri-Urban Beijing, China Based on Fuzzy Set Theory

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Pedosphere 2006年第5期16卷 545-554页

作者： TAN Man-Zhi xu Fang-ming CHEN jie ZHANG xue-Lei CHEN jing-Zhong State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China） Graduate school of the Chinese Academy of Sciences Beijing 100039 Chengdu University of Technology Chengdu 610059 （China） Nanjing Station of Soil and Fertilizer Nanjing 210036 （China）

Fuzzy classification combined with spatial prediction was used to assess the state of soil pollution in the peri-urban Beijing area. Total concentrations of As, Cr, Cd, Hg, and Pb were determined in 220 topsoil samples （0-20 cm） collected using a grid design in a study area of 2 600 kin2. Heavy metal concentrations were grouped into three classes according to the optimum number of classes and fuzziness exponent using the fuzzy comean （FCM） algorithm. Membership values were interpolated using ordinary kriging. The polluted soils of the study area induced by the measured heavy metals were concentrated in the northwest corner and eastern part, especially the southeastern part close to the urban zone, whereas the soils free of pollution were mainly distributed in the southwestern part. The soils with potential risk of heavy metal pollution were located in isolated spots mainly in the northern part and southeastern corner of the study region. The FCM algorithm combined with geostatistical techniques, as compared to conventional single geostatistical kriging methods, could produce a prediction with a quantitative uncertainty evaluation and higher reliability. Successful prediction of soil pollution achieved with FCM algorithm in this study indicated that fuzzy set theory had great potential for use in other areas of soil science.

关键词： continuous classification fuzzy c-means heavy metal soil pollution spatial prediction

Initiation of Acupoint Molecular Mechanisms for Manual Acupuncture Analgesia—Nuclear FactorκB Signaling Pathway

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Chinese Journal of Integrative Medicine 2021年第12期27卷 933-939页

作者： ZHANG Kuo ZHAO xue YANG Tao ZHANG Yan-fang YAN Ya-wen xu Zhi-fang GONG Yi-nan LIU Yang-yang GUO Yong-ming GUO Yi Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjin(300072)China Research Center of Experimental Acupuncture Science Tianjin University of Traditional Chinese MedicineTianjin(301617)China Affiliated Hospital of Municipal Institute of Traditional Chinese Medicine of Changzhi City Shanxi Province(046000)China Sanhuai Road Community Health Service Center Tanggu StreetBinhai New AreaTianjin(300451)China School of Traditional Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin(301617)China

Objective To identify the prominent molecular signaling in acupoints and explore their roles in initiating the analgesia effect of manual acupuncture(MA).Method A three-step study was conducted,the experiment 1 was a genome-wide analysis of the tissue at acupoint Zusanli(ST 36),including 12 Wistar rats which were divided into control,control+MA1,and control+MA7 *** the experiment 2,the paw withdrawal latency(PWL),immunohistochemistry and Western blot analysis of phospho-nuclear factor kappa B(NFκB)p65(p-p65),phospho-NFκB p50(p-p50)at ST 36 were performed on rats of saline,saline+MA,and complete Freund’s adjuvant(CFA)+MA groups(n=6).In experiment 3,24 rats were divided into saline+DMSO,CFA+DMSO,CFA+DMSO+MA,and CFA+BAY 11–7082+MA groups,the PWL and immunofluorescence assay of NFκB p65 at ST 36 was ***(1)The gene:inhibitor of NFκB(Nfkbia),interleukin-1β(Il1b),interleukin-6(Il6),chemokine c-x-c motif ligand 1(Cxcl1),monocyte chemoattractant protein-1(MCP-1/Ccl2)expressions in the control+MA7 group were significantly increased(P<0.05 or P<0.01),and the expression of NFκB p65(Rela),NFκB p50(Nfkb1)were increased in the control+MA7 group(P<0.05).(2)CFA+MA groups showed increased PWL from day 1 to 7(P<0.01 ***),and the Western blot results were consistent with immunohistochemistry,the expression of NFκB p-p65 and NFκB p-p50 were significantly increased in the MA-related groups compared with control and CFA groups(P<0.05).(3)Compared with the CFA+DMSO+MA group,the PWL of the CFA+BAY 11–7082+MA group decreased significantly and continued until day 5 and 7(P<0.05 and P<0.01,respectively),and the NFκB p65 expression of CFA+BAY 11–7082+MA was significantly reduced compared with CFA+DMSO+MA(P<0.01).Conclusion Local NFκB signaling cascade in acupoint caused by MA is an important step in initiating the analgesic effect,which would provide new evidence for the initiation of MA-effect and improve the understanding of the scientific basis of acupuncture analgesia.

关键词： manual acupuncture acupoint analgesia nuclear factor kappa B

Clinical experience in diagnosis and treatment of glucagonoma syndrome

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Hepatobiliary & Pancreatic Diseases International 2004年第3期3卷 473-475页

作者： Min Zhang Xiao xu Yan Shen Zhen-Hua Hu Li-ming Wu Shu-Sen Zheng Department of Hepatobiliary Surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China.

BACKGROUND: Pancreatic endocrine tumors are uncommon neoplasms and can lead to systemic disorder including glucagonoma syndrome, a very rare prototypical paraneoplastic phenomenon. The aim of this study was to assess the diagnosis and surgical strategy for the treatment of glucagonoma syndrome.METHODS: The clinical data of a case of pancreatic head tumor with typical glucagonoma syndrome of necrolytic migratory erythema (NME), diabetes mellitus (DM), anemia, and glossitis were retrospectively analyzed.RESULTS: Cutaneous eruption occurred mainly in the groin, extremities, thighs, buttocks, and perineum. A highly elevated level of serum glucagon was detected by radioimmunoassay. A tumor located in the head of the pancreas was well-defined by pre and intra-operative ultrasonography, contrast enhanced computed tomography, and magnetic resonance imaging. Tumor enucleation was performed, showing significantly improved symptoms. Near complete resolution of NME was shown one week after surgery. Surgical complications or recurrence was not found.CONCLUSIONS: The diagnosis of glucagonoma syndrome is established by marked clinical features such as NME as the hallmark clinical finding, hyperglucagonemia, and radiographically demonstrated neuroendocrine tumor. The topographic diagnosis of glucagonoma can be achieved by combined imaging methods. Enucleation of tumor is a valuable treatment for solitary pancreatic tumor without peripancreatic invasion, liver metastasis, and pancreatic duct compression.

关键词： islet cell tumor diagnosis treatment

Pathological significance of tRNA-derived small RNAs in neurological disorders

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Neural Regeneration Research 2020年第2期15卷 212-221页

作者： Chuan Qin Pei-Pei xu Xin Zhang Chao Zhang Chang-Bin Liu De-Gang Yang Feng Gao ming-Liang Yang Liang-jie Du jian-Jun Li School of Rehabilitation Medicine Capital Medical UniversityBeijingChina China Rehabilitation Science Institute BeijingChina Center of Neural Injury and Repair Beijing Institute for Brain DisordersBeijingChina Department of Spinal and Neural Functional Reconstruction China Rehabilitation Research CenterBeijingChina Beijing Key Laboratory of Neural Injury and Rehabilitation BeijingChina

Non-coding RNAs(ncRNAs) are a type of RNA that is not translated into proteins. Transfer RNAs(tRNAs), a type of ncRNA, are the second most abundant type of RNA in cells. Recent studies have shown that tRNAs can be cleaved into a heterogeneous population of ncRNAs with lengths of 18–40 nucleotides, known as tRNA-derived small RNAs(tsRNAs). There are two main types of tsRNA, based on their length and the number of cleavage sites that they contain: tRNA-derived fragments and tRNA-derived stress-induced RNAs. These RNA species were first considered to be byproducts of tRNA random cleavage. However, mounting evidence has demonstrated their critical functional roles as regulatory factors in the pathophysiological processes of various diseases, including neurological diseases. However, the underlying mechanisms by which tsRNAs affect specific cellular processes are largely unknown. Therefore, this study comprehensively summarizes the following points:(1) The biogenetics of tsRNA, including their discovery, classification, formation, and the roles of key enzymes.(2) The main biological functions of tsRNA, including its miRNA-like roles in gene expression regulation, protein translation regulation, regulation of various cellular activities, immune mediation, and response to stress.(3) The potential mechanisms of pathophysiological changes in neurological diseases that are regulated by tsRNA, including neurodegeneration and neurotrauma.(4) The identification of the functional diversity of tsRNA may provide valuable information regarding the physiological and pathophysiological mechanisms of neurological disorders, thus providing a new reference for the clinical treatment of neurological diseases. Research into tsRNAs in neurological diseases also has the following challenges: potential function and mechanism studies, how to accurately quantify expression, and the exact relationship between tsRNA and miRNA. These challenges require future research efforts.

关键词： epigenetics molecular biology neurological disorders review sequencing stress tRNA tRNA-derived fragments tRNA-derived small RNAs tRNA-derived stress-induced RNA

MicroRNA-145 plays a role in mitochondrial dysfunction in alveolar epithelial cells in lipopolysaccharide-induced acute respiratory distress syndrome

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World Journal of Emergency Medicine 2021年第1期12卷 54-60页

作者： Yi Han Su-cheng Mu jian-li Wang Wei Wei ming Zhu Shi-lin Du Min Min Yun-jie xu Zhen-ju Song Chao-yang Tong Emergency Department Zhongshan HospitalFudan UniversityShanghai 200032China

BACKGROUND: Acute respiratory distress syndrome (ARDS) causes substantial *** epithelium is one of the main sites of cell injuries in ARDS. As an important kind of microRNAs(miRNAs), microRNA-145 (miR-145) has been studied in various diseases, while its role in ARDS has notbeen ***: Lipopolysaccharide (LPS) was intratracheally instilled to establish a rat ARDS *** from bronchoalveolar lavage fl uid (BALF) were measured using rat tumor necrosis factor-α andinterleukin-6 enzyme-linked immunosorbent assay kits (R&D Systems), and the pathological structureswere evaluated using hematoxylin and eosin (H&E) staining and transmission electron microscope;thelung miR-145 messenger RNA (mRNA) was detected using quantitative polymerase chain *** focused on the target genes and possible pathways of gene ***: A rat model of LPS-induced ARDS was successfully established. The miR-145was down-regulated in the LPS-induced ARDS lung, and mitochondrial dysfunction was observedin alveolar epithelial cells, most obviously at 72 hours after LPS. TargetScan and miRDB databaseswere used to predict the target genes of miR-145. A total of 428 overlapping genes were identifi ed,seven genes were associated with mitochondrial function, and Ogt, Camk2d, Slc8a3, and Slc25a25were verifi ed. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were enriched in themitogen-activated protein kinase (MAPK) signaling pathway, and Gene Ontology (GO) biologicalprocess was mainly enriched in signal transduction and transcription ***: The miR-145 is down-regulated in LPS-induced ARDS, and affects itsdownstream genes targeting mitochondrial functions.

关键词： distress injuries alveolar

Observation of whispering-gallery mode splitting in spherical resonator

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Optoelectronics Letters 2007年第3期3卷 231-233页

作者： LIN Guo-ping ZHANG Lei MALe SUN ming-hao xu Hui-ying CAI Zhi-ping Department of Physics Xiamen University Xiamen 361005 China Department of Electronic Engineering Xiamen University Xiamen 361005 China

We report the observation of the splitting of whispering-gallery mode in the silica spherical resonator. To characterize the fused-silica spherical resonator,an evanescent fiber taper coupling technique is employed. The coupling system consists of a fiber taper with a diameter of about 2.8 microns and a fused-silica spherical resonator with a diameter of about 143 microns. By injecting the laser power into the fiber taper,a number of narrow absorption peaks with equal peak spacing of 3.7 nm are observed in a wide spectral range. After replacing the resonator by the other one with the shape being slightly modified,whispering-gallery mode splitting in the spherical resonator is observed. The wavelength spacing between adja-cent peaks is 0.32 nm,much smaller than that of the previous one.

关键词：二氧化硅球形光学谐振器回音壁模式分裂

Coexistence of High Fibrinogen and Low High-density Lipoprotein Cholesterol Levels Predicts Recurrent Cerebral Venous Thrombosis

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Chinese Medical Journal 2015年第13期128卷 1732-1737页

作者： Xin Ma xun-ming ji Paul Fu Yu-Chuan Ding Qiang xue Yue Huang Department of Neurology Xuan Wu Hospital Capital Medical University Beijing 100053 China Department of Neurosurgery Xuan Wu Hospital Capital Medical University Beijing 100053 China Department of Neurosurgery Wayne State University School of Medicine Detroit MI 48202 USA Department of Medical Care for Cadres Beijing Jishuitan Hospital Beijing 100035 China Department of Neurology Beijing Bo Ai Hospital Beijing 100068 China

Background： Cerebral venous thrombosis （CVT） may lead to serious neurological disorders; however, little is known about the risk factors for recurrent CVT. Our aim was to determine the association between elevated fibrinogen and decreased high-density lipoprotein cholesterol （HDL-C） levels with recurrent CVT. Methods： This retrospective cohort study included participants if they had a first episode of objectively defined CVT and were admitted to xuan Wu Hospital, Capital Medical University from August 2005 to September 2009. Demographic and clinical variables were collected, as well as laboratory parameters, inchiding plasma fibrinogen and HDL-C. Patients with CVT were tbllowed for recurrent symptomatic CVT. Follow-up was through the end of September 2010. Potential predictors of recurrence were analyzed using Cox survival analysis. Results： At tile end of the lbllow-up, 95 patients were eligible lbr the study. Twelve of 95 patients （12.6%） had recurred CVT. Tile median time of recurrence was 7 months （range： 1-39 months）. Eight of these 12 （66.7%） experienced rectirrence within the first 12 months alter their initial CVT. The recurrence rate of CVT was 2.76 per 100 patient-years. Multivariate Cox regression analysis demonstrated that the coexistence of high fibrinogen （〉4.00 g/L） and low HDL-C （〈1.08 mmol/L） levels at baseline was the only independent predictor for recurrent CVT （hazard ratio： 4.69; 95% confidence interval： 1.10-20,11; P 〈 0.05）. Of tile twelve patients with recurrent CVT in our study, 7 （58.3%） had high fibrinogen plus low HDL-C levels. All 7 of these patients took warfarin for 3-12 months, and 6 of 7 had rectirrent CVT after the discontinuation of anticoagulant treatment. Conclusions： Concomitant high fibrinogen and low HDL-C levels may be associated with recurrence of CVT. The ett＇ect of potential risk lhctors related to atherothrombosis on rectinent CVT should be closely monitored.

关键词： Cerebral Venous Thrombosis Fibrinogen High-density Lipoprotein Cholesterol Recurrence: Risk Factor

Control strategy for sulfur dioxide and acid rain pollution in China

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Journal of Environmental Sciences 2000年第4期12卷 385-393页

作者： HAO ji ming, WANG Shu xiao, LIU Bing jiang, HE Ke bin (Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.E\|mail:hjm\|den@***) 清华大学北京 100084

Several factors, namely, coal dominated primary energy mix, extensive economic development mode, inefficient energy utilization, and the imperfect environmental regulations, result in the serious urban sulfur dioxide pollution and large scale sulfate type acid precipitation in China. In 1995, China′s sulfur dioxide emissions reached 23.70 Mt, and the areas affected by acid rain accounted for 40% of the territory. Chinese government accords considerable importance to the sulfur dioxide and acid rain contamination. New sets of environmental friendly policies have been promulgated. But enforcement of laws and regulations on SO 2 emissions need to be further improved and broadened, especially those respond to market conditions. This paper focuses particular attention on the analysis of strategy, policies, and national actions which had or should be taken against sulfur dioxide emissions nationwide to achieve the environmental targets, on the basis of which gives the technical options in future.

关键词： sulfur dioxide acid rain control