检索结果-南通市图书馆

Topic identification based on multi-semantic relation fusion

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Journal of Library Science in China 2019年 141-142页

作者： xu Haiyun WU Huawei LUO rui DONG Kun LI Jing 不详

One of the typical characteristics of big data analysis is multivariate data relation *** multi-relationship analysis of topics refers to the analysis of the relationships established between topics and other measurab... 详细信息

关键词： Topic identification multi-semantic

EGCG inhibits cardiomyocyte apoptosis in pressure overload-induced cardiac hypertrophy and protects cardiomyocytes from oxidative stress in rats

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Acta Pharmacologica Sinica 2007年第2期28卷 191-201页

作者： rui SHENG~(2,3,4) zhen-Iun GU~(2,3,4) Mei-lin XIE Wen-xuan ZHOU~3 Ci-yi GUO~32 Department of Pharmacology,Medical School of Soochow University Suzhou 215123 3 Suzhou Institute of Chinese Materia Medica,Suzhou 215007 China Department of Pharmacology Medical School of Soochow University Suzhou China Suzhou Institute of Chinese Materia Medica Suzhou China

Aim:To investigate the effects of epigallocatechin gallate（EGCG）on pressureoverload and hydrogen peroxide（H2O2）induced cardiac myocyte ***:Cardiac hypertrophy was established in rats by abdominal *** 25,50 and 100 mg/kg were administered intragastrically（ig）.Cultured newborn rat cardiomyocytes were preincubated with EGCG,and oxida-tive stress injury was induced by ***:In cardiac hypertrophy inducedby AC in rats,relative to the model group,EGCG 25,50 and 100 mg/kg ig for 6weeks dose-dependently reduced systolic blood pressure（SBP）and heart weightindices,decreased malondialdehyde（MDA）content,and increased superoxidedismutase（SOD）and glutathione peroxidase（GSH-PX）activity,both in serumand in the ***,treatment with EGCG 50 and 100 mg/kg markedlyimproved cardiac structure and inhibited fibrosis in HE and van Gieson（VG）stain,and reduced apoptotic myocytes in the hypertrophic myocardium detected byterminal transferase-mediated dUTP-biotin nick end-labeling（TUNEL）*** Western blot analysis,EGCG significantly inhibited pressure overload-inducedp53 increase and bcl-2 *** H2O2-induced cardiomyocyte injury,whenpreincubated with myocytes for 6-48 h,EGCG 12.5-200 mg/L increased cell viabil-ity determined by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）*** also attenuated H2O2-induced lactate dehydrogenase（LDH）release and MDA formation,Meanwhile,EGCG 50 and 100 mg/L significantlyinhibited the cardiomyocyte apoptotic rate in flow ***:EGCGinhibits cardiac myocyte apoptosis and oxidative stress in pressure overload in-duced cardiac ***,EGCG prevented cardiomyocyte apoptosisfrom oxidative stress in *** mechanism might be related to the inhibitoryeffects of EGCG on p53 induction and bcl-2 decrease.

关键词： epigallocatechin gallate hypertrophy apoptosis oxidative stress cardiac myocytes

Hydrogen-Bonding Interactions between 1-Butyl-2,3-dimethylimidazolium Tetrafluoroborate and Dimethyl Sulphoxide

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Hydrogen-Bonding Interactions between 1-Butyl-2,3-dimethylim...

花椒麻味物质的分离纯化和高效液相色谱法的测定

中国化学会第十九届全国化学热力学和热分析学术会议

作者： Yan-zhen zheng Da-Fu Chen rui Guo Zhong-Min Fu College of Bee Science Fujian Agriculture and Forestry University

＜正＞The use of ionic liquids（ILs） is largely hindered due to high polarity,large viscosity,and high cost of *** mixtures of ILs and polar molecular solvents instead of pure ILs could be an effective approach to so...

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来源： cnki会议评论

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中国调味品 2013年第9期38卷 60-64页

作者：芮光伟曹蕊蒋珍菊西华大学生物工程学院成都610039 云南白药集团股份有限公司昆明653100

通过对花椒中麻味物质的分离纯化，将纯化产物作为标准品，建立高效液相色谱（HPLC）的方法定量检测花椒中麻味物质含量。通过HPLC进行检测，花椒麻味物质在浓度范围为1．0～14μg／mL内线性关系良好，回收率在99％--102％，RSD〈5％。

关键词：花椒麻味物质多烯酰胺纯化高效液相色谱

Successful application of nonmyeloablative stem cell transplantation for paroxysmal nocturnal hemoglobinuria

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Chinese Medical Journal 2007年第22期120卷 2056-2058页

作者： xu Wei LI Jian-yong WANG Li YU Hui ZHANG Su-jiang SHENG rui-lan Department of Hematology First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China

Paroxysmal nocturnal hemoglobinuria （PNH） is a consequence of nonmalignant clonal expansion of one or several hematopoietic stem cells that have acquired a somatic mutation of phosphatedalinositol glycae-class A （PIGA） gene leading to deficient glycosyl phosphatidylinositol （GPI） synthesis. The pathophysiology of PNH is an abnormality in the glycolipid-anchored proteins of the membranes of blood cells. The absence of complement control proteins, including decay accelerating factor （DAF, CD55） and membrane inhibitor of reactive lysis （MIRL, CD59）, results in intravascular lysis of the abnormal cells. Patients with PNH manifest not only clinical and laboratory signs of chronic hemolytic anemia, but also formation of cytopenia and venous thromboses.

关键词： nonmyeloablative stem cell transplantation hemoglobinuria, paroxysmal

Dorsal root ganglion neurons promote proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

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Neural Regeneration Research 2015年第1期10卷 119-123页

作者： Pei-xun Zhang Xiao-rui Jiang Lei Wang Fang-min Chen Lin xu Fei Huang Department of Trauma and Orthopedics Peking University People’s Hospital Department of Orthopedics the Affiliated Yantai Hospital of Binzhou Medical University Department of Human Anatomy Binzhou Medical University

Preliminary animal experiments have confirmed that sensory nerve fibers promote osteoblast differentiation, but motor nerve fibers have no promotion effect. Whether sensory neurons pro- mote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells remains unclear. No results at the cellular level have been reported. In this study, dorsal root ganglion neurons （sensory neurons） from Sprague-Dawley fetal rats were co-cultured with bone marrow mesenchymal stem cells transfected with green fluorescent protein 3 weeks after osteo- genic differentiation in vitro, while osteoblasts derived from bone marrow mesenchymal stem cells served as the control group. The rat dorsal root ganglion neurons promoted the prolifera- tion of bone marrow mesenchymal stem cell-derived osteoblasts at B and 5 days of co-culture, as observed by fluorescence microscopy. The levels of mRNAs for osteogenic differentiation-re- lated factors （including alkaline phosphatase, osteocalcin, osteopontin and bone morphogenetic protein 2） in the co-culture group were higher than those in the control group, as detected by real-time quantitative PCR. Our findings indicate that dorsal root ganglion neurons promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells, which pro- vides a theoretical basis for in vitro experiments aimed at constructing tissue-engineered bone.

关键词： nerve regeneration bone marrow mesenchymal stem cells bone osteoblasts ganglion spine neurons co-culture techniques proliferation differentiation real-time quantitative PCR NSFC grants neural regeneration

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近20a南海赤潮的时空分布特征及原因分析

海洋环境科学 2008年第1期27卷 30-32页

作者：吴瑞贞马毅国家海洋局南海环境监测中心广东广州510300

收集整理了自1980~2004年的南海历次赤潮事件,并对这些赤潮事件在时间和空间的分布特征以及赤潮持续时间长度的变化特征进行了分析;探讨了形成南海赤潮时空分布特征和持续时间长度变化的可能原因。文章认为:赤潮在年度和月份分布不均的... 详细信息

收集整理了自1980~2004年的南海历次赤潮事件,并对这些赤潮事件在时间和空间的分布特征以及赤潮持续时间长度的变化特征进行了分析;探讨了形成南海赤潮时空分布特征和持续时间长度变化的可能原因。文章认为:赤潮在年度和月份分布不均的原因主要是气候差异以及各月份水文气象要素的主要演变特征不同;赤潮持续时间延长的原因与南海区气候转暖和广东近岸海域水质变差有关。研究发现赤潮事件的月份分布和赤潮持续时间长度存在历史性变异,有关现象值得管理部门和学术界的注意,而其中原因更有待进一步研究。

关键词：南海赤潮时空分布持续时间变化原因

电感耦合等离子体原子发射光谱法测定高纯铅中砷铋铜锑锡锌铁

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冶金分析 2012年第11期32卷 61-66页

作者：石镇泰罗文蕊白银有色集团西北铅锌冶炼厂

通过采用硫酸沉淀分离基体铅和选择合适的分析线及背景校正方法消除基体和共存元素干扰,实现了高纯铅中痕量杂质元素砷、铋、铜、锑、锡、锌、铁的电感耦合等离子体原子发射光谱法测定。对仪器的各项测定参数进行优化并将所建立的测定... 详细信息

通过采用硫酸沉淀分离基体铅和选择合适的分析线及背景校正方法消除基体和共存元素干扰,实现了高纯铅中痕量杂质元素砷、铋、铜、锑、锡、锌、铁的电感耦合等离子体原子发射光谱法测定。对仪器的各项测定参数进行优化并将所建立的测定方法应用于实际样品分析,结果表明:用本法测定高纯铅标准样品中砷、铋、铜、锑、锡、锌、铁,测定值与认定值相符,测定结果的相对标准偏差均小于7%。

关键词：电感耦合等离子体原子发射光谱法(ICP-AES) 铅痕量元素

Localization of RanBP1 in Early Embryonic Development of Mice

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Journal of Northeast Agricultural University(English Edition) 2020年第2期27卷 116-121页

作者： Hu Xiao-yang Zeng Xiao xu Ying Ding Cong Zhang Dan-dan Jia zhen-zhen Cao Yun-kao Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine College of Veterinary MedicineNortheast Agricultural UniversityHarbin 150030China

RanBP1 is a binding protein of Ran that plays a pivotal role in nucleocytoplasmic *** this study,the localization and possible functions of RanBP1 were examined,during the early embryonic development of *** results showed that RanBP1 was mainly localized in cytoplasm at mitosis interphase,and its concentration was lower in nucleus and the lowest in *** the formation of the spindle in the early embryonic cells,RanBP1 condensed area took the shape of spindle microtubule,the concentration of RanBP1 was low in the site of *** the formation of nucleus,RanBP1 concentrated in nucleus and there were few dots of RanBP1 around the *** dots were lost after the nucleus full *** results showed that RanBP1 had important roles in nucleocytoplasmic transport,spindle formation and nuclear assembly in the early embryonic development of mice.

关键词： RanBP1 confocal microscopy localization mouse early embryonic development