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Molecular Characterization and Expression and DNA Methylation Analyses of a Galectin-Related Protein Gene from Cynoglossus semilaevis

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Journal of Ocean University of China 2018年第5期17卷 1189-1196页

作者： XIU Yunji gan Tian xu Wenteng SHAO Changwei CHEN Songlin Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyYellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture Marine Science and Engineering College Qingdao Agricultural University College of Fisheries and Life Science Shanghai Ocean University

Galectins, a family of β-galactoside-binding proteins, participate in both innate immunity and adaptive immunity. This study identified one novel galectin-related protein from half-smooth tongue sole Cynoglossus semilaevis, which was designated as CsGRP. The full-length cDNA of its encoding gene was 785 bp in length with a 528 bp open reading frame encoding a putative protein of 176 amino acids. The deduced CsGRP contained a putative 131-aa galactoside-binding lectin domain and 3 critical residues responsible for carbohydrate binding （R93, W109 and R114）. Genomic structural analysis revealed that CsGRP consisted of five exons and four intzons. CsGRP showed 68% similarity with Poecilia latipinna GRP and 67% similarity wih Stegastes partitus GRP. CsGRP showed the highest expression level in liver, although its expression was detected in all tested tissues. When challenged with Vibrio harveyi, the expression of CsGRP was significantly down-regulated in liver （P〈 0.05）. In addition, we found that in spleen and kidney of C. semilaevis, the CpG island of CsGRP showed significantly higher （P 〈 0.05） methylation level in disease-resistant family of C. semilaevis （DR-Cs） than in disease-susceptible ones （DS-Cs）. Our results suggested that CsGRP may play important roles in the immune response of C. semilaevis. Moreover, DNA methylation patterns provided valuable data for understanding the relationship between epigenetic regulation and immunity, which would assist the animal genetic research and improve the animal breeding in the future.

关键词： Cynoglossus semilaevis galectin-related protein expression pattern DNA methylation immune response

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Biodegradable Microspheres with Poly(N-isopropylacrylamide) Enriched Surface: Thermo-responsibility, Biodegradation and Drug Release

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Chinese Journal of Polymer Science 2015年第11期33卷 1598-1605页

作者： Yin Yuan 孙培健 xu-dong Shi Zhi-hua gan 王佛松 CAS Key Laboratory of Engineering Plastics Institute of Chemistry Chinese Academy of Sciences the Key Laboratory of Tobacco Chemistry Zhengzhou Tobacco Research Institute China National Tobacco Corporation State Key Laboratory of Organic-inorganic Composites Beijing Laboratory of Biomedical MaterialsCollege of Life Sciences and Technology

Microspheres with thermo-responsible surface were fabricated by PCL-b-PEO-b-PNIPAM triblock copolymers. Thermo-responsible morphological changes of PCL-b-PEO-b-PNIPAM microspheres immersed in aqueous solution at temperatures above the LCST（e.g. 37 ℃） were observed from porous surface structure to compact surface layer. Enzymatic degradation and in vitro drug release results showed that the thermo-responsible surface layer greatly influenced the degradation of microspheres as well as the drug release behavior from microspheres. With the copolymerization of PNIPAM block into PCL-b-PEO copolymers, the drug release could be well regulated by changing temperatures and microspheres composition, which revealed the great potentials of microspheres with thermo-responsible surface for controlled drug release.

关键词： Microspheres Poly(N-isopropylacrylamide) Thermo-sensitive Biodegradation

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Preparation of Highly Dispersed Antimony-doped Tin Oxide Nano-powder via Ion-exchange Hydrolysis of SnCl_4 and SbCl_3 and Azeotropic Drying

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Chinese Journal of Aeronautics 2007年第2期20卷 181-186页

作者： YANG Fen ZHANG xue-jun TIAN Fang WU xu gan Fu-xing Department of Environmental Engineering School of Resource ＆ Environmental Science Wuhan University Wuhan 430079 China Guizhou Province Key Laboratory of the Ferment Engineering ＆ Biological Pharmacy Guizhou University Guiyang 550003 China Chemistry Department Qujing Normal University Qujing Yunnan 655011china State Key Laboratory of Metal Corrosion and Protection Chinese Academy of Science Shenyang 110016 China

Antimony-doped tin hydroxide colloid precipitates have been synthesized by hydrolysis of SnCl4 and SbCl3 using：（1） an ion-exchange hydrolysis to remove chlorine ions, and （2） isoamyl acetate as an azeotropic solvent to obviate water. The obtained dried powder is of high dispersivity without any need for further grinding. The size and dispersivity of the final particles are investigated with the aid of TG-DTA, BET, XRD and TEM. After having calcined, the antimony-doped tin oxide nanopowder possesses a tetragonal rutile structure with high dispersivity, uniform particles and low hard agglomeration.

关键词： antimony-dopod tin oxide ion-exchange iso-amyl acetate dispcrsivity

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Amperometric detection of gold by differential pulse voltammetry using a DNA biosensor

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Rare Metals 2007年第2期26卷 169-175页

作者： gan Ning WANG Zhiying xu Weiming PAN Jianguo The State Key Laboratory Base of Novel Functional Materials and Preparation Science Ninbo University Ningbo 315211 China

A DNA biosensor with [Ru（DA-bpy）3]Cl2（DA-bpy：4,4＇-diamino-2,2＇-bipyridine）（RuL） as the electrochemical probe was prepared on pyrolytic graphite electrode （PGE） through the supramolecular interaction between RuL complex and DNA template. Cyclic voltammetry of RuL-DNA film showed a pair of stable and reversible peaks corresponding to the Ru（Ⅲ）/Ru（Ⅲ） redox potential of-0.165 V versus AglAgCl in pa 7.4 0.1 mol·L^-1Tris-HCl. The electron transfer was expected across the double-strand DNA by an ＂electron tunneling＂ mechanism. When the DNA biosensor was immerged in gold （Ⅲ） buffer solution, the current peak signal （I） of the RuL-DNA supramolecular depressed and △I was linear in the concentration range of Au ion from 1×10^-7 to 2×10^-5 mol·L^-1 with a regression coefficient of 0.9879. The detection limit was 5×10^-8 mol·L^-1. The developed procedures were applied to the analysis of synthetic samples of real materials with good sensitivity and selectivity.

关键词： gold(Ⅲ) determination differential pulse voltammetry (DPV) DNA biosensor

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Simulation and prediction in laser bending of silicon sheet

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Transactions of Nonferrous Metals Society of China 2011年第A1期21卷 188-193页

作者： WANG xu-yue xu Wei-xing xu Wen-ji HU Ya-feng lianG Yan-de WANG lian-ji Key Laboratory for Precision and Non-traditional Machining Technology of the Ministry of Education Dalian University of Technology Dalian 116024 China

The laser bending of single-crystal silicon sheet （0.2 mm in thickness） was investigated with JK701 Nd：YAG laser. The models were developed to describe the beam characteristics of pulsed laser. In order to simulate the process of laser bending, the FEM software ANSYS was used to predict the heat temperature and stress-strain fields. The periodic transformation of temperature field and stress-strain distribution was analyzed during pulsed laser scanning silicon sheet. The results indicate that the mechanism of pulsed laser bending silicon is a hybrid mechanism in silicon bending, rather than a simple mechanism of TGM or BM. This work also gets silicon sheet bent after scanning 6 times with pulsed laser, and its bending angle is up to 6.5°. The simulation and prediction results reach well agreement with the verifying experiments.

关键词： laser bending silicon sheet pulsed laser simulation

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Improving the Young’s modulus of Mg via alloying and compositing–A short review

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Journal of Magnesium and Alloys 2022年第8期10卷 2009-2024页

作者： Hailong Shi Chao xu Xiaoshi Hu Weimin gan Kun Wu Xiaojun Wang National Key Laboratory for Precision Hot Processing of Metals Harbin Institute of TechnologyHarbin 150001PR China Center for Analysis and Measurement Harbin Institute of TechnologyHarbin 150001PR China GEMS at MLZ Helmholtz-Zentrum Hereon Lichtenbergstrsasse 1Garching D-85748Germany School of Materials Science and Engineering Harbin Institute of TechnologyHarbin 150001PR China

Lightweight,high-modulus structural materials are highly desired in many applications like aerospace,automobile and biomedical *** the lightest metallic structural material,magnesium(Mg)has great potential but is limited by its low intrinsic Young’s *** paper reviews the investigations on high-modulus Mg-based materials during the last *** nature of elastic modulus is introduced,and typical high-modulus Mg alloys and Mg matrix composites are ***,Mg alloys enhance Young’s modulus of pure Mg mainly by introducing suitable alloying elements to promote the precipitation of high-modulus second phases in the alloy ***,Mg matrix composites improve Young’s modulus by incorporating high-modulus particles,whiskers and fibers into the Mg *** modulus strengthening effectiveness brought by the two approaches is compared,and Mg matrix composites stand out as a more promising *** addition,two well-accepted modulus prediction models(Halpin-Tsai and Rule of mixtures(ROM))for different Mg matrix composites are *** effects of reinforcement type,size,volume fraction and interfacial bonding condition on the modulus of Mg matrix composites are ***,the existing challenges and development trends of high-modulus Mg-based materials are proposed and prospected.

关键词： Elastic modulus Mg alloy Mg matrix composite Modulus prediction model Mechanical properties

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Electrochemical Atom Transfer Radical Addition of Polychloroalkanes to Olefins Promoted by 4,4-Di-tert-butyl Bipyridine

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Chinese Journal of Chemistry 2023年第13期41卷 1583-1588页

作者： Fei lian Feixian Luo Meng Wang Kun xu Chengchu Zeng Faculty of Environment and Life Beijing University of TechnologyBeijing100124 China Key Laboratory of Ecology and Environment in Minority Areas(Minzu University of China) National Ethnic Affairs CommissionBeijing100081 China

A new electrochemical strategy for the atom transfer radical addition(ATRA)of polychloroalkanes across olefins has been realized by the synergism of paired electrolysis and halogen bonding ***,readily accessible 4,4-di-tert-butyl bipyridine(dtbpy),acting as a halogen bonding acceptor,shifted the reduction potential of C—Cl bonds positively by 110 *** decreased operating potential leads to a wide substrate scope and excellent functional group compatibility.A diverse array of terminal and internal alkenes such as(hetero)aryl olefins,unactive aliphatic olefins,and natural products and drugs-derived olefins were well compatible.

关键词： Electrocatalysis Organohalides Atom transfer radical addition Radical-polar crossover Alkenes

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The Anion Binding Affinity Determines the Strength of Anion Specificities of Thermosensitive Polymers

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Chinese Journal of Polymer Science 2021年第11期39卷 1351-1356页

作者： Lei-Lei lian Si-Yuan xu Hai-Yang Yuan Guang-Ming Liu Hefei National Laboratory for Physical Sciences at the Microscale Key Laboratory of Surface and Interface Chemistry and Energy Catalysis of Anhui Higher Education InstitutesDepartment of Chemical PhysicsUniversity of Science and Technology of ChinaHefei 230026China

In this work,we demonstrate that the strength of anion specificities of thermosensitive polymers is determined by the affinity of direct anion binding to the *** have prepared a series of thermosensitive statistical copolymers with distinct thermoresponsive *** anions can specifically interact with the different types of thermosensitive polymers in very different strengths.A similar strength of specific anion effects on thermoresponsive behaviors can be observed at very different salt concentrations for the different types of thermosensitive polymers.A stronger anion binding to the thermosensitive polymers gives rise to a more obvious anion specificity and vice *** work presented here opens up opportunities for the application of ion binding affinity to modulate the strength of ion specificities of thermosensitive polymers.

关键词： Specific ion effect Polyzwitterion Ion-polymer interaction Thermosensitivity Ion-pairing

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Petrogenesis of the~1.94 Ga Meta-gabbronorites in liangcheng:Implications for Tectonic Evolution of the Khondalite Belt,North China Craton

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Acta Geologica Sinica(English Edition) 2023年第2期97卷 418-435页

作者： lian Guanghui REN Yunwei SHI Jianrong xu Zhongyuan FENG Fan College of Earth Sciences Jilin UniversityChangchun 130061China Key Laboratory of Mineral Resources Evaluation in Northeast Asia Ministry of Natural ResourcesChangchun 130061China Tianjin Center China Geological SurveyTianjin 300170China Precambrian Geological Research Centre China Geological SurveyTianjin 300170China

We investigated the meta-gabbronorites in liangcheng and used detailed petrography,geochemistry,zircon geochronological and in-situ Hf isotopic studies to clarify their formation and metamorphic ages,petrogenesis,tectonic setting and provide constraints on the tectonic evolution of Khondalite Belt(KB).The zircon U-Pb dating results show that the meta-gabbronorites crystallized at~1.94 Ga and were metamorphosed at~1.91–1.89 *** can be subdivided into the low-Mg and high-Mg *** low-Mg meta-gabbronorites contain relatively lower MgO and higher SiO2 contents than high-Mg *** are enriched in light rare earth elements and large ion lithophile elements,depleted in high field strength elements,and exhibit positive(high-Mg meta-gabbronorites)and negative(low-Mg metagabbronorites)Sr and Eu *** zircon in-situεHf(t)of meta-gabbronorites is 0.07–4.12,with Hf model ages(TDM)of 2169–2400 *** meta-gabbronorites in liangcheng originated from the asthenospheric mantle and experienced fractional crystallization of olivine,orthopyroxene,clinopyroxene,and *** were contaminated by the crustal rocks(mainly khondalite series)during ascent,especially for low-Mg *** ridge subduction is the most plausible tectonic setting for meta-gabbronorites,indicating the eastern segment of KB was in a ridge subduction setting at~1.94 Ga following an orogenic thickening event during a prolonged orogenic process.

关键词： meta-gabbronorites Paleoproterozoic liangcheng Khondalite Belt North China Craton

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A Simple Model of Interaction between Journals, Authors and Editorial Board Members

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Chinese Physics Letters 2011年第1期28卷 222-225页

作者： ZOU Sheng-Rong xu Ying-Ying xu Xiu-lian WANG Jian HE Da-Ren College of Information Engineering Yangzhou University Yangzhou 225009 College of Physics Science and Technology Yangzhou University Yangzhou 225009

Recently, quantitative study on complex systems has become an attractive research area for physicists. Human scientific activity, including scientific paper publication, is an important complex system and therefore deserves an investigation. We present a simple model to describe the interaction between journals, authors and editorial board members. In the model the probabilities, with which a journal accepts papers from an author or invites him as an editorial board member, obey normal distributions. However, the most probable value of the latter distribution shifts such that the journal can have higher level authors being its editorial board members. The analytic derivation by the model is in rather good agreement with the empirical observation from the selected fifteen worldwide journals with different impact factors.

关键词： Statistical physics and nonlinear systems

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