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E3 ubiquitin ligase FBXO32 promotes carbonic anhydrase Ⅲ degradation via proteasome-mediated pathway

第十届全国免疫学学术大会

作者： Xiaonan Zhao Shiqian Huang Ailian Du congfeng xu Shanghai Jiaotong University School of Medicine Department of Neurology Tongren Hospital Shanghai Jiaotong University School of Medicine

INTRODUCTION AND OBJECTIVES After binding by acetylcholine released from a motor neuron,a nicotinic acetylcholine receptor at the neuromuscular junction produces a localized end-plate potential,which leads to muscle *** turnover and renewal of acetylcholine receptors contributes to the pathogenesis of myasthenia *** study would like to unveil if and how endoplasmic reticulum(ER)stress is involved in regulating turnover of acetylcholine *** Utilizing ER inducers such as tunicamycin and thapsigargin,we monitored the endocytosis and degradation of acetylcholine receptor in C2C12 myocytes by flow cytometry,confocal microscopy and western blotting,and further tracked the degradation pathway of acetylcholine receptors with diverse *** knockdown of ER stress proteins such as XBP-1 was also applied to confirm the function of ER stress in regulating acetylcholine receptor endocytosis and *** In the present study,we demonstrated that endoplasmic reticulum(ER)stress contributes to acetylcholine receptor degradation in C2C12 *** stress promotes acetylcholine receptor endocytosis and lysosomal degradation,which was dampened by blocking endocytosis or treating with lysosome *** down of ER stress protein XBP-1 inhibited acetylcholine receptor endocytosis and degradation,while rescue assay restored its endocytosis and degradation,confirming the effects of ER stress on promoting endocytosis-mediated degradation of junction acetylcholine *** Our studies identify ER stress as a factor promoting acetylcholine receptor degradation through accelerating endocytosis in muscle *** ER stress and/or endocytosis might provide a novel therapeutic approach for myasthenia gravis.

关键词： Endoplasmic reticulum stress acetylcholine receptor endocytosis protein degradation skeletal muscle cells myasthenia gravis

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E3 ubiquitin ligase FBXO32 promotes carbonic anhydrase Ⅲ de...

第十一届全国免疫学学术大会

作者： Huang Shiqian Zhao Xiaonan Zhang Shuangyan Huang Jiefang xu congfeng Shanghai Institute of Immunology Institutes of Medical SciencesShanghai Jiao Tong University School of Medicine Key Laboratory of Stem Cell Biology Institute of Health SciencesShanghai Institutes for Biological SciencesChinese Academy of Sciences & SJTUSM

Objective:Myasthenia gravis is an autoimmune disorder,typically caused by pathogenic autoantibodies against the postsynaptic acetylcholine receptor in the endplate of skeletal *** previous studies have identified specific insufficiency of carbonic anhydrase III(CA III) in skeletal muscle from myasthenia gravis patients and confirmed that carbonic anhydrase III is critical for acetylcholine receptor homeostasis in neuromuscular junction,which is essential for normal skeletal muscle ***,the mechanism of carbonic anhydrase III deficiency in skeletal muscle remains to be ***:Taking advantage of yeast two-hybrid screening,we discovered FBXO32(MAFbx/Atrogin-1),a muscle-specific E3 ubiquitin ligase,as a potential interacting protein of CA *** then sought to confirm the physical interaction through co-immunoprecipitation in myocyte C2C12 and HEK293 *** expression was used to reveal the effects of FBXO32 expression on the stability and degradation of CA ***,ubiquitination assay was employed to explore the ubiquitination of CA III by ***:E3 ubiquitin ligase FBXO32 was identified as an interacting protein of CA III,and the physical interaction between FBXO32 and CA III was confirmed by co-immunoprecipitation in HEK293 and C2C12 cells,both exogenously and *** addition,augmented expression of FBXO32 led to accordingly more degradation of CA III,and increasingly ubiquitination of CA III was observed then by *** the process,MG132,a proteasome inhibitor,largely obviated the degradation of CA III,while E64 d,a lysosomal protease inhibitor,has no observable effect,supporting the significant role of proteasome-mediated pathway for CA III ***:FBXO32 is a critical regulator of CA III,by promoting CA III degradation through ubiquitination and subsequent proteasome-dependent *** study reveals the function of FBXO32 and highlights its essential role inregulati

关键词： FBXO32 Carbonic anhydrase Ⅲ protein degradation ubiquitination

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制浆造纸废水处理技术研究进展及思考

中国造纸 2024年第4期43卷 177-188页

作者：许从峰郭芳杏胡春吕来广州大学大湾区环境研究院珠江三角洲水质安全与保护教育部重点实验室广东广州510006 广州大学环境科学与工程学院广东广州510006 广州大学乡村振兴研究院广东广州510006

本文首先简要回顾了制浆造纸废水的特点及其对环境的影响,深入讨论了制浆造纸废水处理的传统技术和最新研究成果,随后总结分析了多种技术联合处理制浆造纸废水的优点及潜力,最后提出制浆造纸废水高效耦合处理新技术,并对未来的发展方向... 详细信息

本文首先简要回顾了制浆造纸废水的特点及其对环境的影响,深入讨论了制浆造纸废水处理的传统技术和最新研究成果,随后总结分析了多种技术联合处理制浆造纸废水的优点及潜力,最后提出制浆造纸废水高效耦合处理新技术,并对未来的发展方向进行了展望,以期为高效低耗制浆造纸废水处理技术的实际应用提供一定的参考。

关键词：制浆造纸废水物化法生物法高级氧化法综合处理

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木质纤维素的微生物降解

生物工程学报 2019年第11期35卷 2081-2091页

作者：许从峰艾士奇申贵男袁媛晏磊王伟东黑龙江八一农垦大学生命科学技术学院黑龙江省寒区环境微生物与农业废弃物资源化利用重点实验室

木质纤维素广泛存在于自然界中,因结构复杂,其高效降解需要多种微生物的协同互作,由于参与木质纤维素降解的微生物种类繁多,其协同降解机理尚不完全明确。随着微生物分子生物学和组学技术的快速发展,将为微生物协同降解木质纤维素机制... 详细信息

木质纤维素广泛存在于自然界中,因结构复杂,其高效降解需要多种微生物的协同互作,由于参与木质纤维素降解的微生物种类繁多,其协同降解机理尚不完全明确。随着微生物分子生物学和组学技术的快速发展,将为微生物协同降解木质纤维素机制的研究提供新的方法和思路。笔者前期研究发现,细菌复合菌系在50℃下表现出强大的木质纤维素降解能力,菌系由可分离培养和暂时不可分离培养细菌组成,但是可分离培养细菌没有降解能力。通过宏基因组和宏转录组研究表明,与木质纤维素降解相关的某些基因表达量发生显著变化,通过组学方法有可能更加深入解释微生物协同降解木质纤维素的微生物学和酶学机理。文中从酶、纯培养菌株和复合菌群三个方面综述了木质纤维素微生物降解研究进展,着重介绍了组学技术在解析复合菌群作用机理方面的现状和应用前景,以期为探索微生物群落协同降解木质纤维素的机理提供借鉴。

关键词：木质纤维素复合菌群协同作用降解机理

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补体系统在动脉粥样硬化中的作用

生命的化学 2021年第2期41卷 368-373页

作者：夏国芳许从峰上海交通大学附属第六人民医院上海200233

动脉粥样硬化是由脂质和纤维在动脉内膜下的过度沉积造成的,脂代谢紊乱和免疫功能失衡是其最重要的发病机制。补体系统是固有免疫的重要组成部分,是炎症反应的关键启动因子,补体系统介导的免疫应答在动脉粥样硬化的发生中发挥着重要作... 详细信息

动脉粥样硬化是由脂质和纤维在动脉内膜下的过度沉积造成的,脂代谢紊乱和免疫功能失衡是其最重要的发病机制。补体系统是固有免疫的重要组成部分,是炎症反应的关键启动因子,补体系统介导的免疫应答在动脉粥样硬化的发生中发挥着重要作用。众多研究表明,动脉粥样硬化斑块中存在多种补体成分以及活化产物,提示补体系统的活化和后续的级联反应是参与动脉粥样硬化发生的重要机制。本文就补体在动脉粥样硬化发生中的作用及其分子机制进行简要综述。

关键词：补体系统炎症反应免疫应答动脉粥样硬化心血管疾病

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Enantio-divergent C–H borylation with two different ligands from a single chiral source

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Science China Chemistry 2025年第1期68卷 226-232页

作者： Rongrong Du Senmiao xu State Key Laboratory for Oxo Synthesis and Selective Oxidation Lanzhou Institute of Chemical Physics Chinese Academy of Sciences College of Material Chemistry and Chemical Engineering Key Laboratory of Organosilicon Chemistry and Material TechnologyMinistry of Education Hangzhou Normal University University of Chinese Academy of Sciences

Reported here is an iridium-catalyzed enantio-divergent C（sp3）–H borylation of α-gem-dimethyl groups of trifluoromethyl ketone-derived hydrazones to construct a series of acyclic all-carbon quaternary stereocenters with a high level of chiral induction. Two different chiral bidentate boryl ligands from a common chiral source not only can switch the enantioselectivity but also are complementary in terms of the substrate scope.

关键词： asymmetric catalysis C–H borylation all-carbon quaternary stereocenters synthetic methods

Aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders:progress of experimental models based on disease pathogenesis

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Neural Regeneration Research 2025年第2期20卷 354-365页

作者： Li xu Huiming xu Changyong Tang Department of Neurology The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

Neuromyelitis optica spectrum disorders are neuroinflammatory demyelinating disorders that lead to permanent visual loss and motor *** date,no effective treatment exists as the exact causative mechanism remains ***,experimental models of neuromyelitis optica spectrum disorders are essential for exploring its pathogenesis and in screening for therapeutic *** most patients with neuromyelitis optica spectrum disorders are seropositive for IgG autoantibodies against aquaporin-4,which is highly expressed on the membrane of astrocyte endfeet,most current experimental models are based on aquaporin-4-IgG that initially targets *** experimental models have successfully simulated many pathological features of neuromyelitis optica spectrum disorders,such as aquaporin-4 loss,astrocytopathy,granulocyte and macrophage infiltration,complement activation,demyelination,and neuronal loss;however,they do not fully capture the pathological process of human neuromyelitis optica spectrum *** this review,we summarize the currently known pathogenic mechanisms and the development of associated experimental models in vitro,ex vivo,and in vivo for neuromyelitis optica spectrum disorders,suggest potential pathogenic mechanisms for further investigation,and provide guidance on experimental model *** addition,this review summarizes the latest information on pathologies and therapies for neuromyelitis optica spectrum disorders based on experimental models of aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders,offering further therapeutic targets and a theoretical basis for clinical trials.

关键词： aquaporin-4 experimental model neuromyelitis optica spectrum disorder pathogenesis

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Reducing the measurement errors in nonadiabatic holonomic quantum computers

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 64-70页

作者： Guo-Fu xu Department of Physics Shandong University

Nonadiabatic holonomic quantum computers serve as the physical platform for nonadiabatic holonomic quantum *** quantum computation has entered the noisy intermediate-scale era, building accurate intermediate-scale nonadiabatic holonomic quantum computers is clearly necessary. Given that measurements are the sole means of extracting information, they play an indispensable role in nonadiabatic holonomic quantum computers. Accordingly, developing methods to reduce measurement errors in nonadiabatic holonomic quantum computers is of great importance. However, while much attention has been given to the research on nonadiabatic holonomic gates, the research on reducing measurement errors in nonadiabatic holonomic quantum computers is severely lacking. In this study, we propose a measurement error reduction method tailored for intermediate-scale nonadiabatic holonomic quantum computers. The reason we say this is because our method can not only reduce the measurement errors in the computer but also be useful in mitigating errors originating from nonadiabatic holonomic gates. Given these features,our method significantly advances the construction of accurate intermediate-scale nonadiabatic holonomic quantum computers.

关键词： quantum holonomy quantum computation measurement errors

Mitophagy in acute central nervous system injuries:regulatory mechanisms and therapeutic potentials

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Neural Regeneration Research 2025年第9期20卷 2437-2453页

作者： Siyi xu Junqiu Jia Rui Mao Xiang Cao Yun xu Department of Neurology Nanjing Drum Tower HospitalClinical College of Jiangsu UniversityNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalChinese Academy of Medical Science&Peking Union Medical CollegeNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingJiangsu ProvinceChina State Key Laboratory of Pharmaceutical Biotechnology and Institute of Translational Medicine for Brain Critical Diseases Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory for Molecular Medicine Medical School of Nanjing UniversityNanjingJiangsu ProvinceChina Nanjing Neurology Medical Center NanjingJiangsu ProvinceChina

Acute central nervous system injuries,including ischemic stro ke,intracerebral hemorrhage,subarachnoid hemorrhage,traumatic brain injury,and spinal co rd injury,are a major global health *** optimal therapies and improving the long-term neurological functions of patients with acute central nervous system injuries are urgent *** are susceptible to damage after acute central nervous system injury,and this leads to the release of toxic levels of reactive oxygen species,which induce cell ***,a selective form of autophagy,is crucial in eliminating redundant or damaged mitochondria during these *** evidence has highlighted the significant role of mitophagy in acute central nervous system *** this review,we provide a comprehensive overview of the process,classification,and related mechanisms of *** also highlight the recent developments in research into the role of mitophagy in various acute central nervous system injuries and drug therapies that regulate *** the final section of this review,we emphasize the potential for treating these disorders by focusing on mitophagy and suggest future research paths in this area.

关键词： autophagy intracerebral hemorrhage ischemic stroke mitochondria mitochondrial biogenesis mitochondrial quality control mitophagy spinal cord injury subarachnoid hemorrhage traumatic brain injury

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