检索结果-南通市图书馆

Chinese Science Bulletin 2006年第5期51卷 530-535页

作者： LIU Tao CHEN Long HOU Sen XUE Yonglai feng xizeng Key Laboratory of Bioactive Materials Ministry of Education Collegeof Life Science Nankai University Tianjin 300071 China

A host type non-virus gene delivery car- rier, phenanthroline-β-cyclodextrin derivative host molecule, was produced which can be used as mo- lecular probe. Interactions between DZY-1 and DNA were investigated by electrophoresis assay. Hind III enzyme inhibition assay was carried out using DNA condensates induced by host molecules or host- guest molecule complexes to explore their ability to inhibit enzyme digestion. Micro-structure of DNA condensates induced by host molecules and host-guest molecule complexes was observed by scanning electron microscope (SEM). Our work in- dicates the delivery mechanism of DZY-1 used as a gene delivery carrier and also provides a method to design and produce non-virus gene delivery carriers.

关键词： DNA 邻二氮杂菲-β-环糊精衍生物非病毒性基因传输载体琼脂凝胶电泳扫描电子显微镜相互作用

The deleterious effects and potential therapeutic strategy of fluorene-9-bisphenol on circadian activity and liver diseases in zebrafish and mice

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第11期145卷 13-27页

作者： Shuhui Zhang Xiang Zuo Jialu Luan Huijuan Bai Zhenhua Fu Mingzhu Sun Xin Zhao xizeng feng College of Life Science State Key Laboratory of Medicinal Chemical BiologyThe Key Laboratory of Bioactive MaterialsMinistry of EducationNankai UniversityTianjin 300071China Institute of Robotics&Automatic Information System College of Artificial IntelligenceNankai UniversityTianjin 300071China

Increasing evidence indicates that disturbance of the clock genes,which leads to systemic endocrine perturbation,plays a crucial role in the pathogenesis of metabolic and liver ***-9-bisphenol(BHPF)is utilized in the manufacturing of plastic materials but its biological effects on liver homeostasis remain *** impacts and involved mechanisms of BHPF on the liver diseases,metabolism,and circadian clock were comprehensively studied by zebrafish andmouse *** therapeutic effect of melatonin(MT)was also *** and mousemodels with either acute exposure(0.5 and 1μmol/L,1-4 days post-fertilization)or chronic oral exposure(0.5 and 50 mg/(kg·2 days),30 days)were established with various BHPF ***,we identified a crucial role for estrogenic regulation in liver development and circadian locomotor rhythms damaged by BHPF in a zebrafish *** mice showed chaos in circadian activity through the imbalance of circadian clock component Brain and Muscle Aryl hydrocarbon receptor nuclear translocator-like 1 in the liver and *** liver sexual dimorphic alteration alongwith reduced growth hormone and estrogens played a critical role in damaged glucose metabolism,hepatic inflammation,and fibrosis induced by ***,sleep improvement by exogenous MT alleviated BHPF-related glucose metabolism and liver injury in *** proposed the pathogenesis of metabolic and liver disease resulting from BHPF and promising targeted therapy for livermetabolism disorders associated with endocrine perturbation *** results might play a warning role in the administration of endocrine-disrupting chemicals in everyday life and various industry applications.

关键词： Fluorene-9-bisphenol(BHPF) Melatonin Circadian activity Endocrine perturbation Liver diseases Female behavior Hepatic sexual dimorphism

Rongchang and Xifeng capsules suppress pentylenetetrazole-induced seizures and change rest/wake behavior in zebrafish larvae

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medicine 2019年第6期39卷 875-884页

作者： Zhang Shuhui Xu Jia Wang Chao feng Daofu Dai Liangti Kuang Xiangyu Zhu Shuyi Fang Yongchun Chen Dongyan He Xianghui feng xizeng State Key Laboratory of Medicinal Chemical Biology The Key Laboratory of Bioactive MaterialsMinistry of Education/College of Life ScienceNankai UniversityTianjin 300071China Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation Department of Histology and EmbryologySchool of MedicineNankai UniversityTianjin 300071China The Institute of Robotics and Automatic Information Systems Tianjin Key Laboratory of Intelligent RoboticsNankai UniversityTianjin 300071China Department of General Surgery Tianjin Medical University General HospitalTianjin300052China

OBJECTIVE:To evaluate the effects of Rongchang capsule and Xifeng capsule on pentylenetetrazoleinduced epilepsy in zebrafish larvae and to explore the possible mechanisms behind their ***:We utilized a trajectory tracking system to monitor seizures in zebrafish larva to confirm that certain concentrations of Rongchang capsule and Xifeng capsule produce antiepileptic effects.c-fos expression was assessed by quantitative reverse transcription-polymerase chain reaction to validate the efficacy of the ***/wake behavior and correlation analysis predicted the targets of Rongchang capsule and Xifeng ***:Larval movement times and total distances traveled by zebrafish larvae experiencing pentylenetetrazole(PTZ)-induced seizures were ***(500μg/m L)and Xifeng(200μg/m L)rescued the epileptic behaviors and down-regulated c-fos expression in the brains of larvae,which indicated antiepileptic *** rest/wake behavioral profiles showed that Rongchang and Xifeng differentially decreased rest time at night and increased larval locomotor activities during the *** on correlation between the actions of the two capsules and known compounds,we predicted that they might change rest/wake behaviors by affecting serotonin,GABAergic and histamine signaling ***:The efficacy of Rongchang capsule and Xifeng capsule in alleviating epilepsy-like behaviors and molecular responses was *** study provides insight into the capsules'effect on epilepsy.

关键词： Zebrafish Larva Anticonvulsants Rongchang capsule Xifeng capsule Rest/wake behavior

在线全文

学校读者我要写书评

暂无评论

微流控芯片技术在生命科学研究中的应用

化学进展 2005年第3期17卷 482-498页

作者：王立凯冯喜增南开大学生物活性材料教育部重点实验室天津300071

微流控芯片最初起源于分析化学领域,是一种采用精细加工技术,在数平方厘米的基片,制作出微通道网络结构及其它功能单元,以实现集微量样品制备、进样、反应、分离及检测于一体的快速、高效、低耗的微型分析实验装置。随着微电子及微机械... 详细信息

微流控芯片最初起源于分析化学领域,是一种采用精细加工技术,在数平方厘米的基片,制作出微通道网络结构及其它功能单元,以实现集微量样品制备、进样、反应、分离及检测于一体的快速、高效、低耗的微型分析实验装置。随着微电子及微机械制作技术的不断进步,近年来微流控芯片技术发展迅猛,并开始在化学、生命科学及医学器件等领域发挥重要作用。本文首先简单介绍了微流控芯片制作材料和工艺,然后主要阐述了其在蛋白质分离、免疫分析、DNA分析和测序、细胞培养及检测等方面的应用进展。

关键词：微流控芯片免疫分析 DNA分析细胞培养

Theaflavins ameliorate insulin resistance and anxiety-like behavior induced by propionate combined with high-fat diet

在线全文

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年

作者： Huijuan Bai Qian Yang Xiang Zuo Qian Yu Qili Zhao Yifei Tian Yixin Xu Jiawei Du Xin Zhao xizeng feng College of Life Science， State Key Laboratory of Medicinal Chemical Biology， Nankai University Institute of Robotics & Automatic Information System， College of Artificial Intelligence， Nankai University

Insulin resistance is a key pathological process in the development of type II diabetes， which can also lead to depression， anxiety and other symptoms. With the accelerated pace of life， food with high-energy and additives is becoming more and more common in people's diet. Propionate is a naturally existing short-chain fatty acid salt that has been widely used as a food preservative， but studies have found that low concentrations of propionate can induce insulin resistance. Theaflavins (TFs) are characteristic components of black tea， which have the biological activity of hypoglycemic and lipid-lowering. In the present study， we used propionate combined with high-fat diet to induce insulin resistance in mice and treated them with theaflavins. The results showed that propionate could aggravate insulin resistance and anxiety induced by high-fat diet in mice. TFs alleviated insulin resistance and anxiety induced by propionate combined with high-fat diet by improving the disorder of metabolic gene expression and inflammation in the adipose and liver tissue， as well as neuroinflammation in the brain. Our study suggests that the food additive propionate combined with a high-fat diet may be harmful to organismal health. As a natural drug， TFs have the potential to alleviate insulin resistance induced by propionate combined with high-fat diet.

关键词：

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

疏水蛋白在云母表面的吸附

高等学校化学学报 2015年第1期36卷 110-115页

作者：何佳冯喜增邵学广蔡文生天津化学化工协同创新中心南开大学化学学院分析科学研究中心药物化学生物学国家重点实验室生命科学学院天津300071

采用分子动力学模拟方法研究了疏水蛋白(HFBI)在亲水云母表面的吸附过程.通过6组平行的分子动力学模拟得到了2种不同的稳定吸附结构,即通过N端和通过亲水的α螺旋与表面吸附,得到了5种吸附残基.进一步用自适应偏置力方法计算了所有吸附... 详细信息

采用分子动力学模拟方法研究了疏水蛋白(HFBI)在亲水云母表面的吸附过程.通过6组平行的分子动力学模拟得到了2种不同的稳定吸附结构,即通过N端和通过亲水的α螺旋与表面吸附,得到了5种吸附残基.进一步用自适应偏置力方法计算了所有吸附残基与表面的结合自由能.结果表明,残基Lys是吸附过程的关键残基,即当HFBI通过含有Lys残基的α螺旋与云母表面作用时,其吸附构象最稳定.静电作用是吸附过程的主要驱动力.在该吸附结构中,HFBI的疏水面暴露在溶液中,有效降低了云母表面的润湿性.

关键词：疏水蛋白云母分子动力学模拟自由能计算吸附构象自适应偏置力方法

在线全文

学校读者我要写书评

暂无评论

对调频同步广播的一些思考

广播与电视技术 2001年第11期28卷 117-119页

作者：冯锡增广东省广播电影电视局

我国的调频同步广播试验是1995年在广东人民广播电台属下的羊城交通电台开始的.羊城交通电台的试验在第一年就取得了非常明显的社会效益和经济效益.特别是它的经济效益是如此明显,以至这个台当时的领导都不愿意向有关的同行和盘托出.关... 详细信息

我国的调频同步广播试验是1995年在广东人民广播电台属下的羊城交通电台开始的.羊城交通电台的试验在第一年就取得了非常明显的社会效益和经济效益.特别是它的经济效益是如此明显,以至这个台当时的领导都不愿意向有关的同行和盘托出.关于羊城交通电台的试验方案和系统组成的简单情况曾经在'广播与电视技术'杂志1998年第7期[1]上介绍过,作者同时也提出了一些建议.事实上,羊城交通电台的试验一开始就引起各方面的极大兴趣,一些地方也陆续开始了试验.原广播电影电视部科技司的领导也一直要求对羊城交通电台的试验能够尽快总结和验收.

关键词：调频广播同步广播广播电台

在线全文

学校读者我要写书评

暂无评论

单分子操作技术在核酸研究中的应用

化学通报 2005年第8期68卷 601-607,600页

作者：薛永来冯喜增侯森南开大学生命科学学院天津300071

单分子操作技术,如原子力显微镜技术、光镊技术和单分子荧光光谱技术,能够对单分子局部力进行测量,因而能在单分子水平上研究核酸的弹性性质和机械诱导的结构转变。单分子操作技术已越来越多地应用于相关的核酸研究中,如DNA的打开与修饰... 详细信息

单分子操作技术,如原子力显微镜技术、光镊技术和单分子荧光光谱技术,能够对单分子局部力进行测量,因而能在单分子水平上研究核酸的弹性性质和机械诱导的结构转变。单分子操作技术已越来越多地应用于相关的核酸研究中,如DNA的打开与修饰、DNA 蛋白质相互作用、DNA凝聚、复制和转录。与经典的分子生物学技术相比,单分子操作技术避免了从大量实验结果中取平均的需要,因而可以提供更为详细的生物信息。本文概述了单分子操作技术的原理及其在核酸研究中的应用。

关键词：单分子操作技术核酸局部力单分子荧光操作技术应用蛋白质相互作用分子生物学技术显微镜技术 DNA

在线全文

学校读者我要写书评

暂无评论

原子力显微术在生物研究中的应用

生命科学 2007年第5期19卷 568-574页

作者：陈龙冯喜增南开大学生命科学学院生物活性材料教育部重点实验室天津300071

原子力显微术不仅能够提供样品表面纳米级别分辨率的三维图像数据,而且能够对pN级微小力进行测量,同时将两者结合发展出的TREC(topography and recognition)显微术还能够在进行高分辨成像的同时实现对特定分子的定位。原子力显微术的这... 详细信息

原子力显微术不仅能够提供样品表面纳米级别分辨率的三维图像数据,而且能够对pN级微小力进行测量,同时将两者结合发展出的TREC(topography and recognition)显微术还能够在进行高分辨成像的同时实现对特定分子的定位。原子力显微术的这些特点使之成为生物化学、细胞生物学等生物研究的有利工具。本文主要介绍了原子力显微镜高分辨成像和检测生物分子识别的原理,以及TREC显微术在生物学上的应用。

关键词： AFM成像力的检测单分子识别

科技规划必须考虑到科技发展给体制和管理带来的影响

在线全文

学校读者我要写书评

暂无评论

广播与电视技术 2005年第6期32卷 41-41页

作者：冯锡增广东省广电局

当前正在制定'十一五'规划,规划对今后的发展无疑具有很重要的指导作用.参加制定规划的专家都有本行业的专业知识和成熟的工作经验,应该对本行业的发展趋势有比较深的了解.因而制定规划本身不是特别困难的事情,尽管它需要大量的调查研... 详细信息

当前正在制定'十一五'规划,规划对今后的发展无疑具有很重要的指导作用.参加制定规划的专家都有本行业的专业知识和成熟的工作经验,应该对本行业的发展趋势有比较深的了解.因而制定规划本身不是特别困难的事情,尽管它需要大量的调查研究和资料收集工作,消耗比较多的人力和时间.

关键词：科技发展科技规划 “十一五”规划管理体制指导作用工作经验专业知识发展趋势收集工作制定行业