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Genome Alignment Spanning Major Poaceae Lineages Reveals Heterogeneous Evolutionary Rates and Alters Inferred Dates for KeyEvolutionary Events

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Molecular Plant 2015年第6期8卷 885-898页

作者： xiyin wa ng Jingpeng wang Dianchuan Jin Hui Guo Tae-Ho Lee Tao Liu Andrew H. Paterson Plant Genome Mapping Laboratory University of Athens GA 30602 USA Center for Genomics and Computational Biology North China University of Science and Technology Tangshan Hebei 063000 China College of Life Sciences North China University of Science and Technology Tangshan Hebei 063000 China College of Sciences North China University of Science and Technology Tangshan Hebei 063000 China Department of Plant Biology University of Georgia Athens GA 30602 USA Department of Crop and Soil Science University of Georgia Athens GA 30602 USA Department of Genetics University of Georgia Athens GA 30602 USA

Multiple comparisons among genomes can clarify their evolution, speciation, and functional innova- tions. To date, the genome sequences of eight grasses representing the most economically important Poaceae （grass） clades have been published, and their genomic-level comparison is an essential foundation for evolutionary, functional, and translational research. Using a formal and conservative approach, we aligned these genomes. Direct comparison of paralogous gene pairs all duplicated simultaneously reveal striking variation in evolutionary rates among whole genomes, with nucleotide substitution slowest in rice and up to 48% faster in other grasses, adding a new dimension to the value of rice as a grass model. We reconstructed ancestral genome contents for major evolutionary nodes, potentially contributing to understanding the divergence and speciation of grasses. Recent fossil evidence suggests revisions of the estimated dates of key evolutionary events, implying that the pan-grass polyploidization occurred ~96 million years ago and could not be related to the Creta- ceous-Tertiary mass extinction as previously inferred. Adjusted dating to reflect both updated fossil evidence and lineage-specific evolutionary rates suggested that maize subgenome divergence and maize-sorghum divergence were virtually simultaneous, a coincidence that would be explained if poly- ploidization directly contributed to speciation. This work lays a solid foundation for Poaceae transla- tional genomics.

关键词： grasses polyploidy genome alignment evolutionary rates whole-genome duplication

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Mitochondrial therapeutics and mitochondrial transfer for neurodegenerative diseases and aging

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Neural Regeneration Research 2025年第3期20卷 794-796页

作者： Neville ng Michelle Newbery Nicole Miles Lezanne Ooi Molecular Horizons and School of Chemistry and Molecular Bioscience University of WollongongWollongongNSWAustralia

Mitochondrial dysfunction and neurodegeneration:Progressive neurodegenerative diseases affect a significant proportion of the population;in a single year,there are as many as 276 million disabilities and 9 million dea... 详细信息

关键词： degenerative diseases Mitochondrial

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Robust interface and excellent as-built mechanical properties of Ti–6Al–4V fabricated through laser-aided additive manufacturing with powder and wire

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 154-168页

作者： Fei Weng Guijun Bi Youxiang Chew Shang Sui Chaolin Tan Zhenglin Du Jinlong Su Fern Lan ng Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials Ministry of EducationShandong Engineering&Technology Research Center for Superhard MaterialSchool of Materials Science and EngineeringShandong UniversityJinan 250061China Institute of Intelligent Manufacturing Guangdong Academy of SciencesGuangzhou 510095China Singapore Institute of Manufacturing Technology 5 Cleantech Loop636732Singapore

The feasibility of manufacturing Ti-6Al-4V samples through a combination of laser-aided additive manufacturing with powder(LAAM_(p))and wire(LAAM_(w))was explored.A process study was first conducted to successfully circumvent defects in Ti-6Al-4V deposits for LAAM_(p) and LAAM_(w),*** the optimized process parameters,robust interfaces were achieved between powder/wire deposits and the forged substrate,as well as between powder and wire *** characterization results revealed the epitaxial prior β grains in the deposited Ti-6Al-4V,wherein the powder deposit was dominated by a finerα′microstructure and the wire deposit was characterized by lamellar α *** mechanisms of microstructure formation and correlation with mechanical behavior were analyzed and *** mechanical properties of the interfacial samples can meet the requirements of the relevant Aerospace Material Specifications(AMS 6932)even without post heat *** fracture occurred within the interfacial area,further suggesting the robust *** findings of this study highlighted the feasibility of combining LAAM_(p) and LAAM_(w) in the direct manufacturing of Ti-6Al-4V parts in accordance with the required dimensional resolution and deposition rate,together with sound strength and ductility balance in the as-built condition.

关键词： laser-aided additive manufacturing powder deposition wire deposition interfacial characteristic mechanical behavior

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Syntheses,structures,and reactivity of ruthenium(Ⅱ) hydride complexes containing Klui's oxygen tripodal ligand

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Science China Chemistry 2010年第10期53卷 2139-2143页

作者： ng Ho-Yuen LAI Chun-Sing WILLIAMS Ian D. LEUng wa-Hung Department of Chemistry The Hong Kong University of Science and Technology

Treatment of Ru(CO)(Cl)(H)(PPh3)3 with NaLOEt (LOEt-= [CpCo{P(O)(OEt)2}3]-) afforded the hydride complex (PPh3)(CO)-LOEtRu(H) (1), which has been characterized by X-ray crystallography. Similarly, the tricyclohexylphosphine analogue, (PCy3)(CO)LOEtRu(H) (2), was synthesized from Ru(CO)Cl(H)(PCy3)2 and NaLOEt. Treatment of complex 1 with R'SO2N3 afforded the (arylsulfonyl)amido complexes LOEtRu(CO)(PPh3)(NHSO2R) (R = 2,4,6-i-Pr3C6H2 (3), 4-t-BuC6H4 (4)). The crystal structure of complex 3 has been determined. The Ru-N distance and Ru-N-S angle in 3 are 2.076(3) and 126.14(16)°, respectively. Reactions of complex 1 with acids have been studied.

关键词： ruthenium oxygen tripodal ligand hydride complex (arylsulfonyl)amido complex

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Acupuncture transcutaneous electrical nerve stimulation reduces discomfort associated with barostat-induced rectal distension:A randomized-controlled study

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World Journal of Gastroenterology 2013年第3期19卷 381-388页

作者： Wing-wa Leung Alice YM Jones Simon SM ng Cherry YN Wong Janet FY Lee Department of Rehabilitation Sciences The Hong Kong Polytechnic UniversityHung HomHong Kong 999077China Faculty of Health Sciences The University of SydneySydneyNSW 2006Australia Division of Colorectal Surgery Department of SurgeryThe Chinese University of Hong KongPrince of Wales HospitalShatinHong Kong 999077China

AIM:To explore the effectiveness of acupuncture transcutaneous electrical nerve stimulation(Acu-TENS), a non-invasive modality in reduction of rectal discomfort during barostat-induced rectal distension. METHODS:Forty healthy subjects were randomized to receive 45 min of either Acu-TENS or placebo-TENS(no electrical output)over acupuncture points Hegu(largeintestine 4),Neiguan(pericardium 6)and Zusanli(stomach 36).A balloon catheter attached to a dual-drive barostat machine was then inserted into the subjects'rectum.A step-wise(4 mmHg)increase in balloon pressure was induced until maximal tolerable or 48 *** analogue scale and a 5-point subjective discomfort scale(no perception,first per-ception of distension,urge to defecate,discomfort/ pain and extreme pain)were used to assess rectal discomfort at each distension *** beta-endorphin levels were measured before,immediately after intervention,at 24 mmHg and at maximal toler- able distension pressure. RESULTS:There was no difference in the demographic data and baseline plasma beta-endorphin levels between the two *** threshold levels were higher in the Acu-TENS group when compared to the placebo group,but the difference reached statistical significance only at the sensations"urge to defecate"and"pain".The distension pressures recorded at the"urge to defecate"sensation for the Acu-TENS and placebo-TENS groups were 28.0±4.5 mmHg and 24.6±5.7 mmHg,respectively(P=0.043);and the pressures recorded for the"pain"sensation for these two groups were 36.0±4.2 mmHg and 30.5± 4.3 mmHg respectively(P=0.002).Compared to the placebo group,a higher number of participants in the Acu-TENS group tolerated higher distension pressures (>40 mmHg)(65%in Acu-TENS vs 25%in placebo, P=0.02).The plasma beta-endorphin levels of the Acu-TENS group were significantly higher than that of the placebo group at barostat inflation pressure of 24 mmHg(1.31±0.40 ng/mL vs 1.04±0.43 ng/mL,P= 0.044)and at maximal inflation p

关键词： Colonoscopy Rectal discomfort Transcu-taneous electrical nerve stimulation Acupuncture Visceral pain

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无线传感器网络分布式同步协议

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西安电子科技大学学报 2016年第4期43卷 105-110,134页

作者：王晶张帅高丹王营冠中国科学院上海微系统与信息技术研究所无线传感网与通信重点实验室上海201899

针对无线传感器网络同步问题,提出分布式时间同步和分布式数据同步的解决方法.前者要求簇头网络进行局部信息交互,并采用低通滤波技术去除噪声干扰;后者为节点提供网络数据均值,要求簇头网络执行比例一致性算法,簇头在迭代过程中引入簇... 详细信息

针对无线传感器网络同步问题,提出分布式时间同步和分布式数据同步的解决方法.前者要求簇头网络进行局部信息交互,并采用低通滤波技术去除噪声干扰;后者为节点提供网络数据均值,要求簇头网络执行比例一致性算法,簇头在迭代过程中引入簇内节点数量.实验结果表明,分布式时间同步具备抗噪声能力,该算法在前期收敛速度最快.网格状网络和随机网络实验表明,分布式时间同步和分布式数据同步的通信开销非常低,它们的收敛速度均高于普通数据同步.

关键词：无线传感器网络同步分簇通信开销收敛速度

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Interaction between enteric epithelial cells and Peyer's patch lymphocytes in response to Shigella lipopolysaccharide: Effect on nitric oxide and IL-6 release

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World Journal of Gastroenterology 2006年第24期12卷 3895-3900页

作者： Jie Chen Chuen-Pei ng Dewi K Rowlands Peng-Hui Xu Jie-Ying Gao Yiu-wa Chung Hsiao-Chang Chan Epithelial Cell Biology Research Center Department of Physiology Faculty of Medicine Chinese University of Hong Kong Hong Kong China Department of Immunology Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 China Department of Biology Faculty of Medicine ShanxiMedical University Taiyuan 030001 Shanxi Province China

AIM： TO investigate the effect of interaction between enteric epithelial cells and lymphocytes of Peyer＇s patch on the release of nitric oxide （NO） and IL-6 in response to Shigella lipopolysaccharide （LPS）. METHODS： Human colonic epithelial cells （Caco-2） were mixed cocultured with lymphocytes of Peyer＇s patch from wild-type （C57 mice） and inducible NO synthase knockout mice, and challenged with Shigella F2a-12 LPS. Release of NO and raiL-6 was measured by Griess colorimetric assay and enzyme-linked immunosorbent assay （ELISA）, respectively. RESULTS： In the absence of LPS challenge, NO was detected in the culture medium of Caco-2 epithelial cells but not in lymphocytes of Peyer＇s patch, and the NO release was further up-regulated in both cocultures with lymphocytes from either the wild-type or iNOS knockout mice, with a significantly higher level observed in the coculture with iNOS knockout lymphocytes. After Shigella F2a-12 LPS challenge for 24-h, NO production was significantly increased in both Caco-2 alone and the coculture with lymphocytes of Peyer＇s patch from the wild-type mice but not from iNOS knockout mice. LPS was found to stimulate the release of mIL-6 from lymphocytes, which was suppressed by coculture with Caco-2 epithelial cells. The LPS-induced mIL-6 production in lymphocytes from iNOS knockout mice was significantly greater than that from the wild-type mice. CONCLUSION： Lymphocytes of Peyer＇s patch maintain a constitutive basal level of NO production from the enteric epithelial cell Caco-2. LPS-induced mIL-6 release from lymphocytes of Peyer＇s patch is suppressed by the cocultured epithelial cells. While no changes are detectable in NO production in lymphocytes from both wild-type and iNOS knockout mice before and after LPS challenge, NO from lymphocytes appears to play an inhibitory role in epithelial NO release and their own mIL-6 release in response to LPS.

关键词： Shigella F2a-12 LPS Colon epithelial cells(Caco-2) Peyer's patch lymphocyte Coculture Nitricoxide Interleukin-6

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针对α网的最优线性约束转换方法

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西安电子科技大学学报 2015年第5期42卷 183-187,212页

作者：张丽赵良煦王寿光汪成英浙江工商大学信息与电子工程学院浙江杭州310018

针对不可控影响子网为α网的一类Petri网,提出了将给定的广义互斥约束转换成最优允许线性约束的方法.该方法首先获得了该网的不可控影响子网;其次,提出了转换后的禁止库所集集合的求解算法;最后,根据禁止库所集集合构造了"逻辑或"形式... 详细信息

针对不可控影响子网为α网的一类Petri网,提出了将给定的广义互斥约束转换成最优允许线性约束的方法.该方法首先获得了该网的不可控影响子网;其次,提出了转换后的禁止库所集集合的求解算法;最后,根据禁止库所集集合构造了"逻辑或"形式的最大允许线性约束.并且通过一个例子,说明了该方法的有效性.

关键词： Petri网离散事件系统禁止状态不可控变迁

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RLN衰落下D-MIMO系统遍历容量上边界

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西安电子科技大学学报 2016年第4期43卷 111-116,153页

作者：王咏平曹汉强王安稳华中科技大学电子信息与通信学院湖北武汉430074 海军工程大学理学院湖北武汉430033

针对空域双边相关同时存在、收发天线数量不受限制的RLN衰落下的分布式多输入多输出系统,分析和提出了解析的系统遍历容量的上边界.通过子矩阵展开等方法构造出新的不等式,在对上述系统模型的遍历容量边界分析中,解决了对大尺度衰落矩... 详细信息

针对空域双边相关同时存在、收发天线数量不受限制的RLN衰落下的分布式多输入多输出系统,分析和提出了解析的系统遍历容量的上边界.通过子矩阵展开等方法构造出新的不等式,在对上述系统模型的遍历容量边界分析中,解决了对大尺度衰落矩阵和小尺度衰落矩阵的统计分析无法分离的问题.当系统接收端天线数量大于或小于等于发送端时,在低信噪比到高信噪比的范围内,上边界均非常接近遍历容量.这一结论由不同空域相关系数下的仿真试验所证实.当分布式系统的收发端站点数量均为1时,模型退化为点对点多输入多输出系统,该上边界变为空域双边相关Rayleigh衰落下多输入多输出系统遍历容量的上边界.

关键词：通信系统衰落信道多输入多输出分布式系统遍历容量

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Interaction of oblique waves with an array of long horizontal circular cylinders

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Science China(Technological Sciences) 2007年第4期50卷 490-509页

作者： ng Chiu-On Department of Mechanical Engineering the University of Hong Kong Pokfulam Road Hong Kong China

The scattering and radiations of linear oblique waves by multiple long horizontal circular cylinders submerged in water of finite depth are investigated using the multipole expansion method. Analytical expressions for the diffracted and radiated potentials are given as a linear combination of infinite multipoles. The unknown coefficients in the expressions are determined by using the addition theorem of the Bessel function and the cylinder boundary conditions. Also analytical expressions for wave forces, hydrodynamic coefficients and reflection and transmission coeffi-cients are derived. The present analytical solution is verified through the boundary element method and applied to investigate three different cases of the interaction of oblique waves with multiple submerged horizontal circular cylinders. The results show that the number of cylinders, the arrangement and spacing between cylinders play an important role in wave forces, hydrodynamic coefficients and reflection and transmission coefficients. Some interesting and important phenomena are ob-served in numerical experiments.

关键词： scattering and radiation water waves analytical technique hydrodynamic characteristics

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