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Journal of Environmental Sciences 2024年第3期137卷 567-579页

作者： xiuying liu Qianna Xia Jiao Zhou Bowen Li Shuaiqi Zhao Long Chen Aimal Khan Xiaoxia Li Aihua Xu School of Chemistry and Chemical Engineering Wuhan Textile UniversityWuhan 430200China Hubei Key Laboratory of Biomass Fibers and Eco-Dyeing and Finishing Wuhan Textile UniversityWuhan 430200China School of Environmental Science and Engineering Huazhong University of Science and TechnologyWuhan 430074China

The design of efficient heterogeneous catalysts in bicarbonate-activated hydrogen peroxide systems(BAP)is a hot topic in wastewater *** this work,Cu_(2)O nanoparticles with different morphologies including cubic shape(c-Cu_(2)O),octahedron shape(o-Cu_(2)O)and spherical shape(s-Cu_(2)O),were applied in BAP for the first time to degrade tetracycline hydrochloride(TC).Compared with Cu^(2+)ions and CuO,TC degradation was boosted in the presence of Cu_(2)O in the BAP system,with the degradation rate following the order c-Cu_(2)O>o-Cu_(2)O>s-Cu_(2)*** morphology-dependent effects could be linearly correlated with the ratio of surface oxygen species(O_S),but not with the surface area or Cu(Ⅰ)*** c-Cu_(2)O catalyst with exposure of(100)facets contained 76.6%O_Sas the active site for H_(2)O_(2)adsorption and activation,while the value was much lower for o-Cu_(2)O and s-Cu_(2)O with dominant(111)*** presence of HCO_(3)-enhanced the interactions among Cu_(2)O,H_(2)O_(2)and TC,leading to facile oxidation of Cu(Ⅰ)to Cu(Ⅱ)by H_(2)O_(2),and the formation of various reactive species such as hydroxyl radicals and Cu(Ⅲ)contributed to TC *** work provides a new method for enhancing H_(2)O_(2)activation with heterogeneous catalysts by crystal facet engineering.

关键词： Hydrogen peroxide Bicarbonate Cu_(2)O Morphology-dependent effects

闽南文化视域下女排精神融入高校德育工作路径探析一以闽南师范大学实践经验为个案

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闽台文化研究 2024年第2期 114-120页

作者：刘秀英闽南师范大学学生工作处福建漳州363000

女排精神是中华体育精神的一面旗帜,生动诠释了祖国至上、团结协作、顽强拼搏、永不言败的精神内涵,与闽南文化德育内涵有着内在的共通性。进入数字时代,数字泛娱乐主义思潮潜移默化地影响着当代青年大学生的主流价值取向。闽南文化视... 详细信息

女排精神是中华体育精神的一面旗帜,生动诠释了祖国至上、团结协作、顽强拼搏、永不言败的精神内涵,与闽南文化德育内涵有着内在的共通性。进入数字时代,数字泛娱乐主义思潮潜移默化地影响着当代青年大学生的主流价值取向。闽南文化视域下传承和弘扬女排精神,有助于匡正大学生价值取向、丰富高校育人资源、弘扬中华体育精神。通过总结闽南师范大学实践经验,提出线下与线下、虚拟与实在、校内与校外相联动的融合路径,不断强化智能式育人、场景化育人、实践式育人效果,实现闽南文化精神与中华体育精神共通共融。

关键词：闽南文化女排精神德育融合路径闽南师范大学

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大豆皂苷结构及生理活性研究进展

食品科学技术学报 2024年第1期42卷 69-82页

作者：朱力杰潘月影李英燕周英杰白昕雯刘秀英刘贺渤海大学食品科学与工程学院/生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁锦州121013 武汉轻工大学食品科学与工程学院湖北武汉430023

大豆皂苷大量存在于大豆的胚芽、胚轴等部分,尤其是在脱脂豆粕中含量丰富,可以作为一类高附加值的产品进行生产,提高大豆油加工副产物的利用率。大豆皂苷属于五环三萜类化合物,是一种齐墩果烷型皂苷,由疏水性苷元和亲水性糖基组成,具有... 详细信息

大豆皂苷大量存在于大豆的胚芽、胚轴等部分,尤其是在脱脂豆粕中含量丰富,可以作为一类高附加值的产品进行生产,提高大豆油加工副产物的利用率。大豆皂苷属于五环三萜类化合物,是一种齐墩果烷型皂苷,由疏水性苷元和亲水性糖基组成,具有一定的两亲性,并具有抗肿瘤、抗炎、抗氧化、调节免疫力、保护肝脏等多种生理活性。近年来,大豆皂苷的研究领域从活性验证到分子机制不断拓展,产出了一批具有前瞻性的研究成果。不同结构与类型的大豆皂苷表现出的生理活性及作用机制不同,相关研究为大豆皂苷在食品和医药等领域的应用提供了理论基础。重点阐述了大豆皂苷的结构与分类、生理活性及构效关系的最新研究进展,分析了加工方式对大豆皂苷结构的影响,总结了大豆皂苷发挥生理活性的机制及其基于生理活性的应用,剖析了大豆皂苷在未来应用中亟待解决的问题,以期为大豆皂苷进一步研究和开发利用提供理论依据。

关键词：大豆皂苷化学结构生物合成生理活性构效关系

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细胞免疫治疗载体技术的现状与展望

中国生物工程杂志 2024年第2期44卷 142-152页

作者：刘秀盈刘静静崔鑫铭于梦圆史渊源王建勋北京中医药大学生命科学学院北京100029 深圳细胞谷生物医药有限公司深圳518000

近年我国细胞免疫治疗发展迅速,从零基础直追国际前沿水平。在细胞免疫疗法蓬勃发展的背后,将基因导入靶细胞并使其进行表达的基因递送载体技术的支持不可或缺。如何更加安全高效地进行基因转递也是困扰行业发展的重要瓶颈之一。通过总... 详细信息

近年我国细胞免疫治疗发展迅速,从零基础直追国际前沿水平。在细胞免疫疗法蓬勃发展的背后,将基因导入靶细胞并使其进行表达的基因递送载体技术的支持不可或缺。如何更加安全高效地进行基因转递也是困扰行业发展的重要瓶颈之一。通过总结目前细胞治疗领域主要应用的载体技术发展现状,并对比已经上市产品的工业化生产流程,以期为后续载体技术的发展提供参考。

关键词：细胞免疫治疗基因递送载体工业化生产

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表达PD-1 shRNA增强靶向CD19 CAR-T细胞对肿瘤细胞的杀伤能力

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中国肿瘤生物治疗杂志 2024年第7期31卷 655-661页

作者：林伟朱晶晶刘秀盈王建勋北京中医药大学生命科学学院北京102400

目的:设计和构建表达PD-1 shRNA的靶向CD19 CAR-T细胞并验证其体外肿瘤细胞杀伤能力。方法:设计并构建表达PD-1 shRNA的CD19 CAR分子基因,将其包装成逆转录病毒载体,通过qPCR法检测病毒载体拷贝数。将慢病毒转导人原代T细胞,获得三种CA... 详细信息

目的:设计和构建表达PD-1 shRNA的靶向CD19 CAR-T细胞并验证其体外肿瘤细胞杀伤能力。方法:设计并构建表达PD-1 shRNA的CD19 CAR分子基因,将其包装成逆转录病毒载体,通过qPCR法检测病毒载体拷贝数。将慢病毒转导人原代T细胞,获得三种CAR-T细胞,分别为RNAU6-CD19 CAR-T、PD-1 shRNA1-CD19 CAR-T、PD-1 shRNA2-CD19 CAR-T细胞。采用qPCR法检测三种CAR-T细胞中PD-1 mRNA的表达水平,流式细胞术检测三种CAR-T细胞中PD-1表达水平,萤光素酶报告基因实验、流式细胞术检测在不同效靶比时CAR-T细胞对CD19阳性靶细胞(人淋巴瘤daudi细胞)的杀伤功能。结果:RNAU6-CD19 CAR、PD-1 shRNA1-CD19 CAR、PD-1 shRNA2-CD19 CAR三种CAR分子成功包装成逆转录病毒载体,病毒载体拷贝数均高于1×10^(7)拷贝/mL,转导人原代T细胞获得CAR-T细胞,RNAU6-CD19 CAR-T、PD-1 shRNA1-CD19 CAR-T、PD-1 shRNA2-CD19 CAR-T细胞转导效率分别为43.1%、55.1%、41.7%。与RNAU6-CD19 CAR-T细胞相比,PD-1 shRNA1-CD19 CAR-T、PD-1 shRNA2-CD19 CAR-T细胞中PD-1 mRNA表达水平均显著降低(均P<0.01)、细胞表面PD-1表达水平更低(均P<0.01)、体外对daudi细胞的杀伤率更高(P<0.05或P<0.01)。结论:成功构建表达PD-1 shRNA的靶向CD19 CAR-T细胞,其对CD19阳性靶细胞的杀伤率显著提高,PD-1 mRNA及其翻译产物PD-1的表达减少,CAR-T细胞的耗竭减缓。

关键词： CD19 PD-1 嵌合抗原受体 T细胞 RNA干扰

Comparison of Skull Morphology in Two Species of Genus liua(Amphibia:Urodela: Hynobiidae),*** and ***

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Asian Herpetological Research 2016年第2期7卷 112-121页

作者： Jianli XIONG xiuying liu Xiaomei ZHANG Animal Science and Technology College Henan University of Science and Technology Key Laboratory of Bio-resources and Eco-environment Ministry of EducationSchool of Life SciencesSichuan University School of Agriculture Henan University of Science and Technology

Skull characteristics play an important role in the systematics of tailed salamanders, in this study, the skulls ofliua shihi and L. tsinpaensis were compared using a clearing and double-staining technique. The results showed that in L. tsinpaensis, the vomerine tooth rows are in a ＂＂ shape, the length of the inner vomerine tooth series is nearly equal to that of the outer series, the vomerine tooth rows do not extend beyond the choanae, an ossified articular bone is absent, the basibranchial is rod shaped, the radial loops exhibit a figure-eight shape, the cornua has two cylindrical branches, the urohyal is rod shaped, and the end of the ceratohyal is not ossified; these features differ considerably from those of L. shihi. The ossification of the posterior portion of the ceratohyal and the present or absent of ossified articular might represent ecological adaptation to feeding in different environments.

关键词： liua shihi liua tsinpaensis morphology adaptation systematics

北京市丙型肝炎病毒感染危险因素的病例对照研究

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国际病毒学杂志 2024年第1期31卷 37-41页

作者：邱倩王怀刘秀颖张卫庞星火北京市疾病预防控制中心科研与教学管理办公室 100013 北京市疾病预防控制中心免疫预防所 100013 北京市疾病预防控制中心中心办公室 100013

目的分析北京地区既往丙型肝炎(丙肝)病毒(hepatitis C virus,HCV)感染者的危险因素,为明确北京市丙肝筛查重点人群提供依据.方法于2021年7月1日至2022年6月30日以北京市7家医院为研究场所,开展1:1配对的病例-对照研究.病例组为调查点... 详细信息

目的分析北京地区既往丙型肝炎(丙肝)病毒(hepatitis C virus,HCV)感染者的危险因素,为明确北京市丙肝筛查重点人群提供依据.方法于2021年7月1日至2022年6月30日以北京市7家医院为研究场所,开展1:1配对的病例-对照研究.病例组为调查点在研究期间新报告的HCV抗体阳性者,对照组为与病例居住相同街(乡)、性别相同和年龄相差5岁以内的健康者.采用logistic回归模型进行危险因素的单因素分析,采用条件logistic回归模型分析HCV感染的危险因素结果研究共纳入感染者、对照组各581人.多因素分析结果输血史(OR=14.59,95%CI:6.16~34.54)、共用注射器史(OR=8.55,95%CI:1.67~43.68)、日常密切接触者中有丙肝患者(OR=2.06,95%CI:1.14~3.74)和手术治疗史(OR=1.84,95%CI:1.24~2.74)与既往HCV感染有较强关联.不同年龄组多因素分析显示,输血史为≤40岁组仅有的独立危险因素,>40岁组中手术治疗史、输血史、日常密切接触者中有丙肝患者、和共用注射器史是独立危险因素.结论手术治疗、输血和共用注射器可能是北京市既往HCV感染的主要危险因素,应以上述人群为重点人群加强丙肝筛查力度,从而发现更多潜在的HCV感染者,同时应对静脉药瘾人群和中老年人群加强HCV健康宣贯,提高人群整体认知水平和自我防护能力,以进一步降低北京地区HCV的人群感染情况.

关键词：丙型肝炎病毒危险因素病例对照研究

Structural Insights into Substrate Selectivity, Catalytic Mechanism,and Redox Regulation of Rice Photosystem Ⅱ Core Phosphatase

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Molecular Plant 2019年第1期12卷 86-98页

作者： xiuying liu Jingchao Chai Xiaomin Ou Mei Li Zhenfeng liu National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of Sciences Beijing 100101P.R.China University of Chinese Academy of Sciences Beijing 100049P.R.China

Photosystem Ⅱ (PSII)core phosphatase (PBCP)selectively dephosphorylates PSII core proteins including D1,D2,CP43,and *** function is required for efficient degradation of the D1 protein in the repair cycle of PSII,a supramolecular machinery highly susceptible to photodamage during oxygenic *** we present structural and functional studies of PBCP from Oryza sativa (OsPBCP).In a symmetrical homodimer of OsPBCP,each monomer contains a PP2C-type phosphatase core domain,a large motif characteristic of PBCPs,and two Small motifs around the active *** large motif contributes to the formation of a substrate-binding surface groove,and is crucial for the selectivity of PBCP toward PSII core proteins and against the light-harvesting ***,the phosphatase activity of OsPBCP is strongly inhibited by glutathione and H202.S-Glutathionylation of cysteine residues may introduce steric hindrance and allosteric effects to the active ***,these results provide detailed mechanistic insights into the substrate selectivity,redox regulation,and catalytic mechanism of PBCP.

关键词： PSII core phosphatase structure substrate selectivity redox regulation PSII repair cycle

Bioinformatic analysis of microRNA biogenesis and function related proteins in eleven animal genomes

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Journal of Genetics and Genomics 2009年第10期36卷 591-601页

作者： xiuying liu GuanZheng Luo Xiujuan Bai Xiu-Jie Wang College of Animal Science and Technology Northeast Agricultural University Harbin 150030 China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Graduate University of Chinese Academy of Sciences Beijing 100101 China d College of Life Science Northeast Agricultural University Harbin 150030 China

MicroRNAs are -22 nt long small non-coding RNAs that play important regulatory roles in eukaryotes. The biogenesis and functional processes of microRNAs require the participation of many proteins, of which, the well studied ones are Dicer, Drosha, Argonaute and Exportin 5. To systematically study these four protein families, we screened 11 animal genomes to search for genes encoding above mentioned proteins, and identified some new members for each family. Domain analysis results revealed that most proteins within the same family share identical or similar domains. Alternative spliced transcript variants were found for some proteins. We also examined the expression patterns of these proteins in different human tissues and identified other proteins that could potentially interact with these proteins. These findings provided systematic information on the four key proteins involved in microRNA biogenesis and functional pathways in animals, and will shed light on further functional studies of these proteins.

关键词： microRNA Drosha Dicer Argonaute Exportin 5

Discovery of an Internasal Bone in Hynobius maoershanensis(Urodela:Hynobiidae)

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Asian Herpetological Research 2011年第2期2卷 87-90页

作者： Jianli XIONG xiuying liu Xiaomao ZENG Animal Science and Technology College Henan University of Science and Technology School of Agriculture Henan University of Science and Technology Chengdu Institute of Biology Chinese Academy of Sciences

Internasal bones have generally been recorded in teleostean and tetrapod fossils, being absent in most extant vertebrates. Presently, there has been one case of the internasal bone discovered in a living salamander, Pseudohynobius puxiongensis （Caudata： Hynobiidae）. The second case discovered in living salamanders is reported in this article, which is present in one of five specimens ofHynobius maoershanensis, a species endemic to China. This case is again regarded as individual variation. Thus, the presence of an ＂internasal bone＂ may represent intraspecific variation and is thus a misleading taxonomic character.

关键词： salamander intemasal bone skull anatomy individual variation