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Stellate ganglion block reduces inflammation and improves neurological function in diabetic rats during ischemic stroke

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Neural Regeneration Research 2022年第9期17卷 1991-1997页

作者： Ting-Ting li Qiang Wan Xin zhang Yuan Xiao li-Ying sun Yu-rong zhang Xiang-Nan liu Wan-Chao Yang Department of Anesthesiology the 2nd Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiang ProvinceChina

Diabetes mellitus is an independent risk factor for ischemic *** diabetes mellitus and stroke are linked to systemic inflammation that aggravates patient *** ganglion block can effectively regulate the inflammatory ***,it is hypothesized that stellate ganglion block could be a potential therapy for ischemic stroke in diabetic *** this study,we induced diabetes mellitus in rats by feeding them a high-fat diet for 4 successive *** left middle cerebral artery was occluded to establish models of ischemic stroke in diabetic ***,we performed left stellate ganglion block with 1%lidocaine using the percutaneous posterior approach 15 minutes before reperfusion and again 20 and 44 hours after *** results showed that stellate ganglion block did not decrease the blood glucose level in diabetic rats with diabetes mellitus but did reduce the cerebral infarct volume and the cerebral water *** also improved the recovery of neurological function,increased 28-day survival rate,inhibited Toll like receptor 4/nuclear factor kappa B signaling pathway and reduced inflammatory response in the plasma of ***,injection of Toll like receptor 4 agonist lipopolysaccharide 5 minutes before stellate ganglion block inhibited the effect of stellate ganglion block,whereas injection of Toll like receptor 4 inhibitor TAK242 had no such *** also found that stellate ganglion block performed at night had no positive effect on diabetic ischemic *** findings suggest that stellate ganglion block is a potential therapy for diabetic ischemic stroke and that it may be mediated through the Toll like receptor 4/nuclear factor kappa B signaling *** also found that the therapeutic effect of stellate ganglion block is affected by circadian rhythm.

关键词： circadian rhythms diabetes mellitus inflammation ischemic stroke long-term prognosis neurological function neuron nuclear factor kappa B stellate ganglion block Toll like receptor 4

Generation of low-cadmium rice germplasms via knockout of OsLCD using CRISPR/Cas9

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Journal of Environmental Sciences 2023年第4期126卷 138-152页

作者： Huamei Chen rong Ye Ying liang Shuchang zhang Xiulian liu Chongjun sun Fangbai li Jicai Yi College of life Sciences South China Agricultural UniversityGuangzhou 510642China Institute of Eco-environmental and Soil Sciences Guangdong Academy of SciencesGuangzhou 510650China

The OsLCD gene,which has been implicated in cadmium (Cd) accumulation in rice,might be a useful target for CRISPR/Cas9 ***,the effects of Os LCD gene editing on Cd accumulation,plant growth,and yield traits remain ***,we used CRISPR/Cas9to generate oslcd single mutants from indica and japonica rice *** also generated osnramp5 single mutants and oslcd osnramp5 double mutants in the indica *** grown in Cd-contaminated paddy soils,all oslcd single mutants accumulated less Cd than the wild types (WTs).Consistent with this,oslcd single mutants grown in Cd-contaminated hydroponic culture accumulated significantly less Cd in the shoots as compared to *** decrease in accumulation probably resulted from the reduction of Cd translocation under Cd *** damage also decreased,and plant growth increased in all oslcd single mutant seedlings as compared to WTs in the presence of *** growth and most yield traits,as well essential element concentrations in rice seedling shoots,brown rice,and rice straw,were similar between oslcd single mutants and *** the presence of Cd,Cd concentrations in the brown rice and shoots of oslcd osnramp5 double mutants were significantly decreased compared with WTs as well as osnramp single *** results suggested that OsL CD knockout may reduce Cd accumulation alone or in combination with other knockout mutations in a variety of rice genotypes;unlike Os Nramp5 mutations,Os LCD knockout did not reduce essential element ***,Os LCD knockout might be used to generate low-Cd rice germplasms.

关键词： Cadmium Rice OsLCD Crispr/Cas9 editing

Temporal changes in mixing effects on litter decay and nitrogen release in a boreal riparian forest in northeastern China

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Journal of Forestry Research 2024年第2期35卷 33-41页

作者： Simin Wang Bo liu Rui li Xiaoxin sun rong Mao Key Laboratory of National Forestry and Grassland Administration on Forest Ecosystem Protection and Restoration of Poyang Lake Watershed College of ForestryJiangxi Agricultural UniversityNanchang 330045People’s Republic of China Key Laboratory of Wetland Ecology and Environment Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102People’s Republic of China Key Laboratory of Sustainable Forest Ecosystem Management of Ministry of Education School of ForestryNortheast Forestry UniversityHarbin 150040People’s Republic of China Heilongjiang Sanjiang Plain Wetland Ecosystem Research Station Fuyuan 156500People’s Republic of China

In riparian forests,litter decay provides essential energy and nutrients for both terrestrial and fluvial *** mixing effects(LMEs)are crucial in regulating litter decay and nutrient dynamics,yet how LMEs change over time is unclear in riparian *** this study,leaf litter of three common species(Alnus sibirica *** Turcz,Betula platyphylla Sukaczev,and Betula fruticosa Pall.)were mixed in an equal mass ratio and LMEs were measured for mass and nitrogen(N)remaining in whole litter mixtures over a 3-year period in a boreal riparian forest,northeastern *** were also assessed for component litter mass and N remaining by separating litter mixtures by *** the decay of litter mixtures,antagonistic effects on mass and N remaining were dominant after one and two years of decay,whereas only additive effects were observed after three *** correlated negatively with functional diversity after the first and two years of decay but disappeared after three *** sorting litter mixtures by species,non-additive LMEs on mass and N remaining decreased over incubation ***,non-additive LMEs were more frequent for litter of both *** and *** with lower N concentration than for *** litter with higher N *** results indicate that incubation time is a key determinant of litter mixing effects during decay and highlight that late-stage litter mixture decay may be predicted from single litter decay dynamics in boreal riparian forests.

关键词： Biodiversity litter quality litter mixtures Synergistic effects Functional diversity

Adipose tissue macrophages:implications for obesity-associated cancer

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Military Medical Research 2023年第5期10卷 636-651页

作者： Bei li Si sun Juan-Juan li Jing-Ping Yuan Sheng-rong sun Qi Wu Department of Pathology Renmin Hospital of Wuhan UniversityWuhan 430060China Department of Clinical Laboratory Renmin Hospitalof Wuhan UniversityWuhan 430060China Department of Breast and Thyroid Surgery Renmin Hospital of Wuhan UniversityWuhan 430060China Tongji University Cancer Center Shanghai Tenth People’s Hospital of Tongji UniversitySchool of MedicineTongji UniversityShanghai 200092China

Obesity is one of the most serious global health problems,with an incidence that increases yearly and coincides with the development of *** tissue macrophages(ATMs)are particularly important in this context and contribute to linking obesity-related inflammation and tumor ***,the functions of ATMs on the progression of obesity-associated cancer remain *** this review,we describe the origins,phenotypes,and functions of ***,we summarize the potential mechanisms on the reprogramming of ATMs in the obesity-associated microenvironment,including the direct exchange of dysfunctional metabolites,inordinate cytokines and other signaling mediators,transfer of extracellular vesicle cargo,and variations in the gut microbiota and its metabolites.A better understanding of the properties and functions of ATMs under conditions of obesity will lead to the development of new therapeutic interventions for obesity-related cancer.

关键词： Adipose tissue macrophages Macrophage Adipose Obesity Cancer Therapy

Density Functional Theory Study on La Complex with Schiff-base as Building Block

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Chinese Journal of Structural Chemistry 2006年第2期25卷 197-203页

作者： XIA Shu-Wei XU Xiang sun Ya-li FAN Yu-Hua BI Cai-Feng zhang Dong-Mei YANG li-rong Department of Chemistry Ocean University of China Qingdao 266003 China

Quantum density functional theory （DFT） results are reported for the building block [LaL^1（NO3）] of La complex [LaL^1（NO3）]NO3·5H2O （L^1 = （CH3）2CHCH2CH（NCHC4H3O）COO^-）. The structure was optimized and the calculation results show that the lanthanum ion is coordinated by one nitrogen atom and three oxygen atoms of L^1 and two oxygen atoms of nitrate ion. The bond length of La-N is 0.2637 nm and the average length of La-O is 0.2526 nm, which are consistent with the literatures. In addition, the stabilities, electronic structural characteristics and IR spectra of the complex have been analyzed, which describe the coordination of lanthanum ion with other atoms in detail.

关键词： rare-earth Schiff-base lanthanum complex density functional theory

Reversible Mechanochemistry Enabled Autonomous Sustaining of Robustness of Polymers—An Example of Next Generation Self-healing Strategy

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Chinese Journal of Polymer Science 2021年第5期39卷 545-553,I0005页

作者： Ming-Xuan li Min-Zhi rong Ming-Qiu zhang Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education Guangdong Provincial Key Laboratory of High Performance Polymer CompositesSchool of ChemistrySun Yat-Sen UniversityGuangzhou 510275China

Even under low external force,a few macromolecules of a polymer have to be much more highly stressed and fractured first due to the inherent heterogeneous *** the materials keep on working under loading,as is often the case,the minor damages would add up,endangering the safety of *** we show an innovative solution based on mechanochemically initiated reversible cascading variation of metal-ligand *** loading,crosslinking density of the proof-of-concept metallopolymer networks autonomously increases,and recovers after ***,the stress-induced tiny fracture precursors are blocked to grow and then *** entire processes reversibly proceed free of manual intervention and *** proposed molecular-level internal equilibrium prevention mechanisms fundamentally enhance durability of polymers in service.

关键词： Metal-ligand complexations Polyurethane Mechanochemistry Mechanical properties Robustness

Construction and Characterization of Lentiviral shRNA Expression Vector Targeting Rat CD40 Gene in Dendritic Cells

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Chemical Research in Chinese Universities 2009年第5期25卷 666-672页

作者： sun Mei li Jin-dong JIANG Rui JIN Cheng-yan GAO Nan LUO Shu-li WANG Chun-guang WANG bin WANG rong-you zhang Xing-yi Department of Pathology Second Hospital of Jilin University Changehun 130041 P. R. China Department of Thoracic Surgery Second Hospital of Jilin University Changehun 130041 P. R. China Department of Orthopedic Surgery China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China Department of Thoracic Surgery China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China

To construct a lentiviral shRNA vector targeting rat CD40 gene and detect its effectiveness of gene silencing in dendritic cells（DCs）, specific siRNA targets with short hairpin frame were designed and synthesized according to the mRNA sequence of rat CD40 gene. DNA oligo was cloned into lentiviral expression vector, and then PCR and sequencing analyses were conducted to verify the constructs. The verified plasmids were transfected into 293T cells that over-express recombinant CD40 in order to select the most effective siRNA targets, shRNA lentiviruses from the selected constructs were propagated and harvested with a virus packaging system, and the virus titers were determined. Western blot and Real-time PCR were performed to determine CD40 expression level in the virusinfected dendritic cells. PCR and sequencing analyses reveal that shRNA plasmids of four targets were successfully constructed. The optimal interfering target was selected, and the virus with a titer of 5 × 10^7 TU/mL was successfully packaged. CD40 expression in rat DCs was knockdown at both mRNA and protein levels by virus infection. In comparison to that of control groups, CD40 mRNA expression and protein expression were decreased by 60.9% and 61.2%, respectively. We have successfully constructed recombinant lentiviral shRNA expression vector targeting rat CD40 gene that can effectively down-regulate CD40 gene expression at mRNA and protein levels in rat DC.

关键词： CD40 RNA interference Lentivirus Dendritic cell

Sol-Gel Synthesis of Normal Spinel liMn_2O_4 and Its Characteristics

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Journal of Wuhan University of Technology(Materials Science) 2002年第3期17卷 1-4页

作者： LEI Jia-heng sun Yu-bin CHEN Yong-xi WANG Jin-rong XUE li-hui Wuhan University of Technology

Normal spinel liMn 2O 4 was synthesized by sol-gel method using lithium nitrate,manganese nitrate,citric acid and ethylene glycol as raw materials. liMn 2O 4 was characterized by XRD,TG-DTA,IR,SEM and *** optimum conditions for the synthesis were *** acid and ethylene glycol were mixed with molar ratio of 0.25,and the mixture was esterified at 140℃ for 4 *** lithium nitrate and manganese nitrate were added with molar ratio of 0.6. In the system,the total molar of cations was equal to that of citric acid. At last, reflux the system at 105℃ for 2 hours. Dried gel was fired at 600℃ for 8 hours. Particle diameters of raw product were about 100 nm mainly. Further research shows that lithium ion of liMn 2O 4 is easy to be extracted,and normal spinel λ-MnO 2 can be obtained after lithium ion extraction.

关键词： sol gel method synthesis of liMn 2O 4 λ MnO 2

Multi-Omics Analysis Provides Insight into the Possible Molecular Mechanism of Hay Fever Based on Gut Microbiota

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Engineering 2022年第8期8卷 115-125页

作者： Pei Han li-sha li Zi-Xi Wang lin Xi Hang Yu lin Cong Zheng-Wei zhang Jie Fu Ran Peng li-bin Pan Shu-rong Ma Xue-Yan Wang Hong-Tian Wang Xiang-Dong Wang Yan Wang Jin-Lyu sun Jian-Dong Jiang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China Department of Allergy National Clinical Research Center for Dematologic and Immunologic DiseasesBeijing Key Laboratory of Precision Medicine for Diagnosis and Treatment on Allergic DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Department of Allergy&Department of Otorhinolaryngology Head and Neck Surgery&Key Laboratory of Otolaryngology Head and Neck Surgery Ministry of EducationBeijing Tongren HospitalCapital Medical UniversityBeijing 100005China Department of Allergy Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Beijing Key Laboratory of Nasal Diseases Beijing Institute of OtolaryngologyBeijing Tongren HospitalCapital Medical UniversityBeijing 100005China

Due to the worldwide epidemic of allergic disease and a cure nowhere in sight,there is a crucial need to explore its pathophysiological *** allergic disease has been associated with gut dysbiosis,we searched for a possible mechanism from the perspective of the molecular interface between host and microbiota with concurrent metabolomics and microbiome composition ***-Dawley rats were injected with Artemisia pollen extract to stimulate a hyper reaction to *** hyper reaction decreased the circulation of valine,isoleucine,aspartate,glutamate,glutamine,indole-propionate(IPA),and myo-inositol,and reduced short-chain fatty acids(SCFAs)in *** beneficial genera belonging to Ruminococcaceae,Lachnospiraceae,and Clostridiales declined in the model group,whereas Helicobacter and Akkermansia were only expressed in the model ***,the expression of intestinal claudin-3 and liver fatty acid binding protein was downregulated in the model group and associated with metabolic changes and *** results suggest that alterations in amino acids as well as their derivatives(especially valine,and IPA which is the reductive product of tryptophan),SCFAs,and the gut microbiome(specifically Akkermansia and Helicobacter)may disrupt the intestinal barrier function by inhibiting the expression of claudin proteins and affecting the mucus layer,which further results in hay fever.

关键词： Metabolome Gut microbiota Hay fever Allergic diseases Intestinal barrier dysfunction