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Laparoscopic, Endoscopic and Robotic Surgery 2024年第1期7卷 6-10页

作者： Raojun Luo Yiming Li xiumin Han Yunzheng Wang Zhengfu He Peijian yan Ziyi Zhu Department of Thoracic Surgery Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Department of Operating Room Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China

Robotic surgery systems,as emerging minimally invasive approaches,have been increasingly applied for the treatment of esophageal cancer because they provide a high-definition three-dimensional surgical view and mechanical rotating arms that surpass the limitations of human hands,greatly enhancing the accuracy and flexibility of surgical methods.Robot-assisted McKeown esophagectomy(RAME),a common type of robotic esophagectomy,has been gradually implemented with the aim of reducing postoperative complications,improving postoperative recovery and achieving better long-term survival.Multiple centers worldwide have reported and summarized their experiences with the RAME,and some have also discussed and analyzed its perioperative effects and survival prognosis compared with those of video-assisted minimally invasive esophagectomy.Compared to traditional surgery,the RAME has significant advantages in terms of lymph node dissection although there seems to be no difference in overall survival or disease-free survival.With the continuous advancement of technology and the development of robotic technology,further development and innovation are expected in the RAME field.This review elaborates on the prospects of the application and advancement of the RAME to provide a useful reference for clinical practice.

关键词： Esophageal cancer Minimally invasive esophagectomy Robot-assisted McKeown esophagectomy Lymph node dissection

Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat

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Journal of Molecular Cell Biology 2019年第5期11卷 383-394页

作者： Yirong Zhang Yawen Chen Jianqun Zheng Juan Wang Shichao Duan Wei Zhang xiumin yan Xueliang Zhu State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology University of Chinese Academy of Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China

Motile cilia and flagella are microtubule-based organelles important for cell locomotion and extracellular liquid flow through beating. Although axonenal dyneins that drive ciliary beat have been extensively studied in unicellular Chlamydomonas, to what extent such knowledge can be applied to vertebrate is poorly known. In Chlamydomonas, Dynein-f controls flagellar waveforms but is dispensable for beating. The flagellar assembly of its heavy chains (HCs) requires its intermediate chain (IC) IC140 but not IC138. Here we show that, unlike its Chlamydomonas counte『part, vertebrate Dynein-f is essential for ciliary beat. We confirmed that Wdr78 is the vertebrate orthologue of IC3 Wdr78 associated with Dynein-f subunits such as Dnah2 (a HC) and Wdr63 (IC140 orthologue). It was expressed as a motile cilium-specific protein in mammalian cells. Depletion of Wdr78 or Dnah2 by RNAi paralyzed mouse ependymal cilia. Zebrafish Wdr78 morphants displayed ciliopathy-related phenotypes, such as curved bodies, hydrocephalus, abnormal otolith, randomized left-right asymmetry, and pronephric cysts, accompanied with paralyzed pronephric cilia. Furthermore, all the HCs and ICs of Dynein-f failed to localize in the Wdr78-depleted mouse ependymal cilia. Therefore, both the functions and subunit dependency of Dynein-fare altered in evolution, probably to comply with ciliary roles in higher organisms.

关键词： axonemal dynein Dynein-f IC138 motile cilium primary ciliary dyskinesia Wdr78 Dnah2

Microtubule-bundling protein Spef1 enables mammalian ciliary central apparatus formation

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Journal of Molecular Cell Biology 2019年第1期11卷 67-77页

作者： Jianqun Zheng Hao Liu Lei Zhu Yawen Chen Huijie Zhao Wei Zhang Fan Li Lele Xie xiumin yan Xueliang Zhu State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Shanghai 200031China

Cilia are cellular protrusions containing nine microtubule(MT)doublets and function to propel cell movement or extracellular liquid flow through beating or sense environmental stimuli through signal transductions.Cilia require the central pair(CP)apparatus,consisting of two CP MTs covered with projections of CP proteins,for planar strokes.How the CP MTs of such‘9+2’cilia are constructed,however,remains unknown.Here we identify Spef1,an evolutionarily conserved microtubule-bundling protein,as a core CP MT regulator in mammalian cilia.Spef1 was selectively expressed in mammalian cells with 9+2 cilia and specifically localized along the CP.Its depletion in multiciliated mouse ependymal cells by RNAi completely abolished the CP MTs and markedly attenuated ciliary localizations of CP proteins such as Hydin and Spag6,resulting in rotational beat of the ependymal cilia.Spef1,which binds to MTs through its N-terminal calponin.homologous domain,formed homodimers through its C-terminal coiled coil region to bundle and stabilize MTs.Disruption of either the MT-binding or the dimerization activity abolished the ability of exogenous Spef1 to restore the structure and functions of the CP apparatus.We propose that Spefl bundles and stabilizes central MTs to enable the assembly and functions of the CP apparatus.

关键词： central pair apparatus cilium microtubule mouse ependymal cell mouse tracheal epithelial cell Spef1

基于1 610例直肠癌术后非肿瘤复发性吻合口狭窄的危险因素分析及其列线图预测模型的构建

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中华胃肠外科杂志 2024年第6期27卷 600-607页

作者：邱克茂蒋伟郑吉祥冯明远刘秀敏陆丁山严俊南方医科大学附属南方医院普通外科广州

目的评估影响直肠癌术后发生非肿瘤复发性吻合口狭窄的危险因素并构建其列线图风险预测模型。方法采用回顾性观察性研究的方法, 收集南方医科大学南方医院2003年1月至2018年9月期间行手术治疗且一期肠道吻合的直肠癌患者资料。排除标准:... 详细信息

目的评估影响直肠癌术后发生非肿瘤复发性吻合口狭窄的危险因素并构建其列线图风险预测模型。方法采用回顾性观察性研究的方法, 收集南方医科大学南方医院2003年1月至2018年9月期间行手术治疗且一期肠道吻合的直肠癌患者资料。排除标准:(1)术后病理提示吻合口切缘有肿瘤残留者;(2)术后肠镜病理提示吻合口狭窄处为肿瘤复发、或术后影像学评估及肿瘤标志物监测等提示肿瘤复发者;(3)随访时间<3个月;(4)同时多原发癌患者。采用χ2检验或Fisher精确检验进行单因素分析, 评价研究对象一般资料以及肿瘤相关因素和手术方式等变量对直肠癌术后发生非肿瘤复发性吻合口狭窄的影响。经单因素分析筛选出P<0.05的变量, 进一步采用向前逐步回归法对P<0.05的变量采用二分类自变量logistic回归进行多因素分析, 筛选出直肠癌术后非肿瘤复发性吻合口狭窄发生的独立危险因素。最后在R软件中进一步构建直肠癌术后非肿瘤复发性吻合口狭窄的列线图预测模型, 使用内部验证和计算模型的受试者工作特征曲线(ROC)的曲线下面积(AUC)来评价该模型预测的可靠性和准确度。结果共纳入1 610例患者, 其中男性1 008例, 女性602例;中位年龄为59(50, 67)岁;中位体质指数22.4(20.2, 24.5)kg/m2;其中发生非肿瘤复发性吻合口狭窄121例(7.5%)。行新辅助化疗、新辅助放化疗和直接手术患者术后的非肿瘤复发性吻合口狭窄发生率分别为11.2%(10/89)、26.4%(47/178)和4.8%(64/1 343)。新辅助治疗(新辅助化疗:OR=2.455, 95%CI:1.148～5.253, P=0.021;新辅助放化疗:OR=3.882, 95%CI:2.425～6.216, P<0.001)、术后吻合口漏(OR=7.960, 95%CI:4.550～13.926, P<0.001)、手术方式为开腹手术(OR=3.412, 95%CI:1.772～6.571, P<0.001)以及原发肿瘤位置(肿瘤下缘距离肛缘5～10 cm:OR=2.381, 95%CI:1.227～4.619, P<0.001;肿瘤下缘距离肛缘<5 cm:OR=5.985, 95%CI:3.039～11.787, P<0.001)均是直肠癌术后非肿瘤复发性吻合口狭窄发生的独立危险因素。基于筛选出的4个独立因素, 建立直肠癌术后吻合口狭窄发生的相关风险因素列线图模型。计算出模型ROC的AUC为0.815(0.773～0.857, P<0.001), 内部验证法显示, 该预测模型的C-index值为0.815, 且该模型校正曲线与理想曲线拟合度良好。结论直肠癌术后非肿瘤复发性吻合口狭窄与新辅助治疗、吻合口漏、手术方式及原发肿瘤位置密切相关, 基于上述4因素的列线图模型具有良好的预测区分度和校正度, 有利于筛查直肠癌术后吻合口非肿瘤复发性狭窄人群和制定针对性的防治措施。

关键词：直肠肿瘤吻合口狭窄, 非肿瘤复发性因素分析预测模型列线图模型

O-GIcNAc transferase regulates centriole behavior and intraflagellar transport to promote ciliogenesis

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Protein & Cell 2020年第11期11卷 852-857页

作者： Fan Yu Te Li yanchao Sui Qingxia Chen Song yang Jia yang Renjie Hong Dengwen Li xiumin yan Wei Zhao Xueliang Zhu Jun Zhou Department of Genetics and Cell Biology College of Lifle SciencesState Key Laboratory of Medicinal Chemical BiologyTanjin Key Laboratory of Protein ScienceKey Laboratory of Bioactive Materials of the Ministry of EducationNankai UniversilyTianjin 300071China College of Pharmacy State Key Laboratory of Medicinal Chemical BiologyNankai UniyersityTianjin 300071China State Key Laboratory of Cell Biology Shanghai Insttute of Biochemistry and Cell BiologyCenter for Excel lence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China Institute of Biomedical Sciences Shandong Provincial Key Lab-oratory of Animal Resistance BiologyCollaboralive Innovation Center of Cell Biology in Universities of ShandongCollge of Life Sciences.Shandong Nomal UniversityJinan 250014China

Dear Editor,O-GIcNAcylation is a nutrient sensor that is particularly sensitive to environmental glucose(Hardiville and Hart,2014).Glucose can be converted to UDP-GIcNAc through the hexosamine biosynthetic pathway,providing a substrate for O-GIcNAcylation.Two enzymes participate in this reversible modification,O-GIcNAc transferase(OGT),which adds a single GIcNAc residue to the serine/threonine sites of proteins,and O-GIcNAcase(OGA),which removes the residue(yang and Qian,2017).

关键词： UDP residue remove

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中药治疗过敏性鼻炎的机制分析

中华中医药杂志 2024年第1期39卷 309-313页

作者：闫盼盼李彦冉刘洋李秀敏苗明三河南中医药大学郑州450046

过敏性鼻炎属于中医“鼻鼽”范畴,主要为免疫球蛋白E介导引起的Ⅰ型变态炎症反应、嗜酸性粒细胞浸润的慢性炎症、辅助性T细胞(Th)1/Th2和Th17/调节性T细胞(Treg)等免疫细胞失衡、细胞间黏附分子水平升高等,发病机制较为复杂。中药防治... 详细信息

过敏性鼻炎属于中医“鼻鼽”范畴,主要为免疫球蛋白E介导引起的Ⅰ型变态炎症反应、嗜酸性粒细胞浸润的慢性炎症、辅助性T细胞(Th)1/Th2和Th17/调节性T细胞(Treg)等免疫细胞失衡、细胞间黏附分子水平升高等,发病机制较为复杂。中药防治过敏性鼻炎已成为目前常用的方法,主要通过减少鼻黏膜炎性浸润、调节免疫与神经系统、降低过敏反应等改善过敏性鼻炎症状,已在动物实验和临床应用中得到证实。近年来,一些学者对中药治疗过敏性鼻炎的作用机制作了分析和探讨,笔者对此作一综述。

关键词：中药治疗中西医过敏性鼻炎药理作用作用机制

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Changing Mortality and Patterns of Death Causes in HIV Infected Patients—China,2013-2022

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China CDC weekly 2023年第48期5卷 1073-1078页

作者： yan Zhao Lai Wei Zhihui Dou Decai Zhao xiumin Gan Yasong Wu Mengjie Han National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina

What is already known about this topic?The advent of antiretroviral therapy(ART)has markedly decreased mortality rates among patients infected with human immunodeficiency virus(HIV).Globally,there has been a 43%reduction in acquired immunodeficiency syndrome(AIDS)-related deaths from 2010 to 2022.Additionally,prior research indicates that the initiation of ART at an early stage within China has substantially lowered mortality rates.What is added by this report?Over the previous decade,following the implementation of China’s universal ART access strategy,the patterns of mortality causes among HIVinfected individuals across the country have undergone significant alterations.In 2022,the all-cause mortality rate among this population was reported at 2.7%,with the non-AIDS-related mortality rate at 1.8%.However,it is important to consider that the accuracy of death reporting could contribute to potential misclassification of the underlying causes of death.What are the implications for public health practice?Efforts to enhance health outcomes should persist in emphasizing the advancement of ART strategies,with a particular focus on mitigating non-AIDS-related mortality in the future.

关键词： markedly alterations Pattern

A Secure Supply of Antiretroviral Medicines for People Living with HIV During the COVID-19 Pandemic—China’s Experience

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China CDC weekly 2022年第38期4卷 849-852页

作者： yan Zhao Mengjie Han Yuting Lian Qiang Wang xiumin Gan Lan Yu Chuntao Ma National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina

The occurrence of acute infectious diseases or severe natural disasters has a negative impact on the medical system(1).A survey of 105 countries by the World Health Organization(WHO)from March to June 2020 found that health services were disrupted in 90%of countries due to the coronavirus disease 2019(COVID-19)pandemic,with low-and middle-income countries being the most affected.Human immunodeficiency virus(HIV)treatment continuity and healthcare service delivery were also affected by the COVID-19 pandemic(2–3).

关键词： acute diseases income

Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approach

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Food Science and Human Wellness 2023年第4期12卷 1224-1231页

作者： Mohan Li Qilong Li Haikun Yu xiumin Zhang Dehao Li Wanying Song yan Zheng Xiqing Yue College of Food Science Shenyang Agricultural UniversityShenyang 110866China College of Animal Science and Veterinary Medicine Shenyang Agricultural UniversityShenyang 110866China Beijing Academy of Food Sciences Beijing 100068China The Fourth Affiliated Hospital of China Medical University Shenyang 110866China

This study aimed to analyze and compare the differentially expressed whey proteins(DEWPs)of donkey and bovine colostrum using high-performance liquid chromatography with tandem mass spectrometry-based proteomics.A total of 620 and 696 whey proteins were characterized in the donkey and bovine colostrum,respectively,including 383 common whey proteins.Among these common proteins,80 were identified as DEWPs,including 21 upregulated and 59 downregulated DEWPs in donkey colostrum compared to bovine colostrum.Gene Ontology analysis revealed that these DEWPs were mainly related to cellular components,such as extracellular exosome,plasma membrane,and mitochondrion;biological processes,such as oxidation-reduction process,cell-cell adhesion,and small guanosine triphosphate(GTP)ase-mediated signal transduction;and molecular functions,such as GTP binding,GTPase activity,and soluble N-ethylmaleimide-sensitive factor(NSF)attachment protein receptor activity.Metabolic pathway analysis suggested that the majority of the DEWPs were associated with soluble NSF factor attachment protein receptor interactions in vesicular transport,fatty acid biosynthesis,and estrogen signaling pathways.Our results provide a vital insight into the differences between donkey and bovine colostrum,along with important information on the significant components as nutritional and functional factors to be included in infant formula based on multiple milk sources.

关键词： Bovine colostrum Donkey colostrum Proteomics Whey protein Gene Ontology Kyoto Encyclopedia of Genes and Genomes

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基于ICEEMDAN与样本熵的脑血氧信号去噪方法

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电子科技 2024年第6期37卷 44-50页

作者：曹焱赵斌邢志明金子豪董祥美高秀敏上海理工大学光电信息与计算机工程学院上海200093

人体生理活动和随机噪声都会对脑血氧检测数据精度产生影响,为提高测量精度,需解决信号采集时遇到的噪声干扰。文中提出一种利用改进的具备自适应噪声的完全集成经验模态分解(Improved Complete Empirical Mode Decomposition with Adap... 详细信息

人体生理活动和随机噪声都会对脑血氧检测数据精度产生影响,为提高测量精度,需解决信号采集时遇到的噪声干扰。文中提出一种利用改进的具备自适应噪声的完全集成经验模态分解(Improved Complete Empirical Mode Decomposition with Adaptive Noise,ICEEMDAN)与样本熵(Sample Entropy,SampEn)相结合的脑血氧信号去噪方法。利用ICEEMDAN对脑血氧信号进行模态分解,从而获得不同时间复杂度的固有模态函数(Intrinsic Mode Function,IMF)分量。通过样本熵值判断各IMF分量的时间复杂度,依据IMF分量的样本熵值选择合适的分量重构信号,从而去除原始信号的噪声。实验结果表明,所提方法可以有效去除原始脑血氧信号中的噪声,实现采集数据的精度提升,进而提高脑血氧检测精度。

关键词：脑血氧精度 ICEEMDAN 样本熵固有模态函数重构信号血氧信号噪声去除

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