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Synthetic and Systems Biotechnology 2022年第2期7卷 689-704页

作者： Mostafa Basiony Liming Ouyang Danni Wang Jiaming Yu Liming Zhou Mohan Zhu Xuyuan Wang Jie Feng Jing Dai Yijie Shen Chengguo Zhang Qiang Hua xiuliang yang Lixin Zhang State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai200237China Shandong Jincheng Bio-Pharmaceutical Co. Ltd.No.117 Qixing River RoadZibo255130ShandongChina

The global market demand for natural astaxanthin is rapidly increasing owing to its safety,the potential health benefits,and the diverse applications in food and pharmaceutical industries.The major native producers of natural astaxanthin on industrial scale are the alga Haematococcus pluvialis and the yeast Xanthopyllomyces dendrorhous.However,the natural production via these native producers is facing challenges of limited yield and high cost of cultivation and extraction.Alternatively,astaxanthin production via metabolically engineered non-native microbial cell factories such as Escherichia coli,Saccharomyces cerevisiae and Yarrowia lipolytica is another promising strategy to overcome these limitations.In this review we summarize the recent scientific and biotechnological progresses on astaxanthin biosynthetic pathways,transcriptional regulations,the interrelation with lipid metabolism,engineering strategies as well as fermentation process control in major native and non-native astaxanthin producers.These progresses illuminate the prospects of producing astaxanthin by microbial cell factories on industrial scale.

关键词： Astaxanthin Carotenoids Metabolic engineering Microbial cell factories Lipid Fermentation Antioxidants

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有限递减变换半群的商半群的一个注记

杭州师范大学学报（自然科学版） 2024年第1期23卷 106-112页

作者：钱承镠杨秀良杭州师范大学数学学院浙江杭州311121

令Sing n为X n={1,2,…,n}上的奇异变换半群,令S-n={α∈Sing n|xα≤x,x∈X n},这里1<2<…

关键词：递减全变换半群同余正则半群富足半群

Phosphate limitation increases coenzyme Q10 production in industrial Rhodobacter sphaeroides HY01

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Synthetic and Systems Biotechnology 2019年第4期4卷 212-219页

作者： Lu Zhang Leshi Liu Ke-Feng Wang Lan Xu Liming Zhou Weishan Wang Chuan Li Zheng Xu Tong Shi Haihong Chen Yuanhang Li Hui Xu xiuliang yang Zhichun Zhu Biqin Chen Dan Li Guanghuang Zhan Si-Liang Zhang Li-Xin Zhang Gao-Yi Tan State Key Laboratory of Bioreactor Engineering(SKLBE) And School of BiotechnologyEast China University of Science and Technology(ECUST)No.130 Meilong RoadShanghai200237China State Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences(CAS)No.1 Beichen West RoadBeijing100101China Institute of Applied Ecology Chinese Academy of SciencesShenyang110016China Shandong Jincheng Bio-Pharmaceutical Co. LtdNo.117 Qixing River RoadZibo255130China Inner Mongolia Kingdomway Pharmaceutical Co. LtdTuoketuo Power Industrial ParkHohhot010206China Department of Biology Beijing Institute of EducationBeijing 100044China

Coenzyme Q10(CoQ10)is an important component of the respiratory chain in humans and some bacteria.As a high-value-added nutraceutical antioxidant,CoQ10 has excellent capacity to prevent cardiovascular disease.The content of CoQ10 in the industrial Rhodobacter sphaeroides HY01 is hundreds of folds higher than normal physiological levels.In this study,we found that overexpression or optimization of the synthetic pathway failed CoQ10 overproduction in the HY01 strain.Moreover,under phosphate-limited conditions(decreased phosphate or in the absence of inorganic phosphate addition),CoQ10 production increased significantly by 12%to220 mg/L,biomass decreased by 12%,and the CoQ10 productivity of unit cells increased by 27%.In subsequent fed-batch fermentation,CoQ10 production reached 272 mg/L in the shake-flask fermentation and 1.95 g/L in a 100-L bioreactor under phosphate limitation.Furthermore,to understand the mechanism associated with CoQ10 overproduction under phosphate-limited conditions,the comparatve transcriptome analysis was performed.These results indicated that phosphate limitation combined with glucose fed-batch fermentation represented an effective strategy for CoQ10 production in the HY01.Phosphate limitation induced a pleiotropic effect on cell metabolism,and that improved CoQ10 biosynthesis efficiency was possibly related to the disturbance of energy metabolism and redox potential.

关键词： R sphaeroides CoQ10 Phosphate limitation Overproduction Scale-up Transcriptome

TaRLK-6A promotes Fusarium crown rot resistance in wheat

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Journal of Integrative Plant Biology 2024年第1期66卷 12-16页

作者： Haijun Qi xiuliang Zhu Wenbiao Shen Xia yang Chaozhong Zhang Genying Li Feng Chen Xuening Wei Zengyan Zhang Institute of Special Animal and Plant Sciences Chinese Academy of Agricultural SciencesChangchun 130000China The National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China College of Life Science Nanjing Agricultural UniversityNanjing 210095China Agronomy College/National Key Laboratory of Wheat and Maize Crop Science/CIMMYT‐China(Henan)Joint Center of Wheat and Maize Henan Agricultural UniversityZhengzhou 450002China Department of Plant Sciences University of CaliforniaDavisCA 95616USA Crop Research Institute Shandong Academy of Agricultural SciencesJinan 250100China

Fusariumcrown rot(FCR),mainly caused by the soil‐borne fungus Fusarium pseudograminearum,is a devastatingdisease of wheat(Triticum aestivum(Ta)).Fusariumcrown rotcauses substantial yield losses and generates mycotoxins inwheat grains that can cause serious health problems in hu-mans and livestock(Powell et al.,2017).Identifying genes thatprovide resistance toF.pseudograminearumand character-izing the molecular mechanisms underlying such resistance arekey to breeding wheat varieties that are resistant to this dev-astating fungus.

关键词： aestivum breeding soil

Remote sensing of quality traits in cereal and arable production systems:A review

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The Crop Journal 2024年第1期12卷 45-57页

作者： Zhenhai Li Chengzhi Fan Yu Zhao xiuliang Jin Raffaele Casa Wenjiang Huang Xiaoyu Song Gerald Blasch Guijun yang James Taylor Zhenhong Li College of Geodesy and Geomatics Shandong University of Science and TechnologyQingdao 266590ShandongChina Key Laboratory of Quantitative Remote Sensing in Ministry of Agriculture and Rural Affairs Information Technology Research CenterBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China Institute of Crop Sciences Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and EcologyMinistry of Agriculture and Rural AffairsBeijing 100081China DAFNE Universitàdella TusciaVia San Camillo de Lellis01100 ViterboItaly Key Laboratory of Digital Earth Science Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100094China International Maize and Wheat Improvement Center(CIMMYT) PO Box 5689Addis AbabaEthiopia ITAP Univ.MontpellierINRAEInstitut AgroMontpellier 34000France College of Geological Engineering and Geomatics Chang’an UniversityXi’an 710054ShaanxiChina

Cereal is an essential source of calories and protein for the global population.Accurately predicting cereal quality before harvest is highly desirable in order to optimise management for farmers,grading harvest and categorised storage for enterprises,future trading prices,and policy planning.The use of remote sensing data with extensive spatial coverage demonstrates some potential in predicting crop quality traits.Many studies have also proposed models and methods for predicting such traits based on multiplatform remote sensing data.In this paper,the key quality traits that are of interest to producers and consumers are introduced.The literature related to grain quality prediction was analyzed in detail,and a review was conducted on remote sensing platforms,commonly used methods,potential gaps,and future trends in crop quality prediction.This review recommends new research directions that go beyond the traditional methods and discusses grain quality retrieval and the associated challenges from the perspective of remote sensing data.

关键词： Remote sensing Quality traits Grain protein Cereal

Research on the Generation Mechanism and Suppression Method of Aerodynamic Noise in Expansion Cavity Based on Hybrid Method

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Computer Modeling in Engineering & Sciences 2024年第6期139卷 2747-2772页

作者： Haitao Liu Jiaming Wang xiuliang Zhang Yanji Jiang Qian Xiao School of Mechanotronics and Vehicle Engineering East China Jiaotong UniversityNanchang330013China Suzhou Automotive Research Institue Tsinghua UniversitySuzhou215131China

The expansion chamber serves as the primary silencing structure within the exhaust pipeline.However,it can also act as a sound-emitting structure when subjected to airflow.This article presents a hybrid method for numerically simulating and analyzing the unsteady flow and aerodynamic noise in an expansion chamber under the influence of airflow.A fluid simulation model is established,utilizing the Large Eddy Simulation(LES)method to calculate the unsteady flow within the expansion chamber.The simulation results effectively capture the development and changes of the unsteady flow and vorticity inside the cavity,exhibiting a high level of consistency with experimental observations.To calculate the aerodynamic noise sources within the cavity,the flow field results are integrated using the method of integral interpolation and inserted into the acoustic grid.The acoustic analogy method is then employed to determine the aerodynamic noise sources.An acoustic simulation model is established,and the flow noise source is imported into the sound field grid to calculate the sound pressure at the far-field response point.The calculated sound pressure levels and resonance frequencies show good agreement with the experimental results.To address the issue of airflow regeneration noise within the cavity,perforated tubes are selected as a means of noise suppression.An experimental platformfor airflow regeneration noise is constructed,and experimental samples are processed to analyze and verify the noise suppression effect of perforated tube expansion cavities under different airflow velocities.The research findings indicate that the perforated tube expansion cavity can effectively suppress low-frequency aerodynamic noise within the cavity by impeding the formation of strong shear layers.Moreover,the semi-perforated tube expansion cavity demonstrates the most effective suppression of aerodynamic noise.

关键词： Expansion cavity aerodynamic noise numerical simulation hybrid method perforated tube

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保序递减变换半群的秩2自同态

杭州师范大学学报（自然科学版） 2023年第1期22卷 82-87,105页

作者：阳晶杨秀良杭州师范大学数学学院浙江杭州311121

令Tn为有限集Xn={1,2,…,n}上的全变换半群.本文刻画了子半群Cn={α∈Tn■x,y∈Xn,x≤y■xα≤yα且xα≤x}上秩2的所有自同态,并得到Cn的秩2的所有自同态的个数.

关键词：保序递减变换半群自同态秩2

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保序递减全变换半群

杭州师范大学学报（自然科学版） 2022年第2期21卷 164-168页

作者：阳晶杨秀良杭州师范大学数学学院浙江杭州311121

令T_(n)为有限集X_(n)={1,2,…,n}上的全变换半群.研究子半群C_(n)={α∈T_(n)|■x,y∈X_(n),x≤y?xα≤yα且xα≤x}.特别得到C_(n)的每一个Gree_(n)关系都是恒等关系,且每一个正则元都是幂等元;进一步C_(n)的每一个L^(*)类和每一个R^... 详细信息

令T_(n)为有限集X_(n)={1,2,…,n}上的全变换半群.研究子半群C_(n)={α∈T_(n)|■x,y∈X_(n),x≤y?xα≤yα且xα≤x}.特别得到C_(n)的每一个Gree_(n)关系都是恒等关系,且每一个正则元都是幂等元;进一步C_(n)的每一个L^(*)类和每一个R^(*)类都仅含唯一个幂等元,但不是L^(*)-幂单的和R^(*)-幂单的.

关键词：保序递减全变换半群 Green关系 Green*关系

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递减全变换半群的幂等元生成集深度

杭州师范大学学报（自然科学版） 2023年第3期22卷 305-311页

作者：钱承镠杨秀良杭州师范大学数学学院浙江杭州311121

令Sing n为X n={1,2,…,n}上全变换半群的奇异部分.X n上递减全变换半群为S-n={α∈Sing n|xα≤x,x∈X n},S-n由幂等元生成,且被J*n-1里的n(n-1)/2个幂等元生成.文章进一步研究了S-n的幂等元深度问题,证明了E(J*n-1)是S-n的所有生成元... 详细信息

令Sing n为X n={1,2,…,n}上全变换半群的奇异部分.X n上递减全变换半群为S-n={α∈Sing n|xα≤x,x∈X n},S-n由幂等元生成,且被J*n-1里的n(n-1)/2个幂等元生成.文章进一步研究了S-n的幂等元深度问题,证明了E(J*n-1)是S-n的所有生成元集的交,给出了α∈S-n的E(J*n-1)-深度和S-n的全局E(J*n-1)-深度,以及α∈S-n的E(S-n)-深度和S-n的全局E(S-n)-深度.

关键词：全变换半群递减全变换半群幂等元深度

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完全正则半群的Cayley表示

数学学报（中文版） 1995年第5期38卷 653-657页

作者：杨秀良贵州黔东南民族师专

本文利用点集映射和等价关系得出了完全正则半群的Ｃａｙｌｅｙ表示，从而解决了１９８７年Ｍ．Ｐｅｔｒｉｃｈ提出的一个公开问题．作为特例，还得到完全单半群的ｃａｙｌｅｙ表示．

关键词： Cayley表示完全正则半群半群