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Soft Substrate Induces Endothelial Cell Inflammation and Disrupts Endothelium Integrity

Journal of Biosciences and Medicines 2021年第2期9卷 92-102页

作者： Yiling Tan xiuli mao Huanli Wang School of Biomedical Engineering Shanghai Jiao Tong University Shanghai China

Atherosclerosis (AS) is the main cause of death and disability all over the world. A lot of efforts have been devoted to treat AS, among which tissue engineering blood vessel materials, including artificial blood vessels, stents and vascular patches, have brought hope to ameliorate the symptoms in AS patients. However, there remains a large percentage of implantation failure due to the incompatibility of the material with the body. AS is a multi-factor related disease, and chronic inflammation is a major event that involves with its pathogenesis and development. Since previous studies suggested that the stiffness of the blood vessel might affect the inflammatory conditions, in this paper, we investigate the mechanism of how substrate stiffness could affect the inflammation response of the endothelial cells (ECs). Polyacrylamide (PA) based hydrogels at different concentrations were used as the culture substrate for ECs. The mRNA expression level of VCAM-1 and ICAM-1 was determined by qRT-PCR. EC chemotactic effect was evaluated by the number of THP-1 adhered to EC monolayer. The protein levels of IκBα and NF-κB were determined by western blotting analysis. The expression and localization of the major adherens junctions (AJs) proteins, VE-cadherin and β-catenin, were evaluated by western blotting and immunofluorescence staining. Our results showed that ECs cultured on soft substrate (1 kPa) demonstrated more chemotactic effect and the amount of the monocytes adhered to them was higher than that on harder substrate (20 kPa, p < 0.05). Moreover, NF-κB signaling pathway in ECs on 1 kPa substrate was more activated compared to those on 20 kPa substrate, with the IκBα protein expression level in the cytoplasm decreasing and NF-κB translocating more into the nuclear. In addition, the AJs of the endothelial monolayer changed with the substrate stiffness. Compared with ECs on normal substrate (20 kPa), the protein expression level of β-catenin decreased (p < 0.05), and immunofluorescence staining of VE-cadherin and β-caten

关键词： Substrate Stiffness Endothelial Cells Inflammation Adherens Junction

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防护工程复合埋管式热泵系统性能分析

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陆军工程大学学报 2024年第2期3卷 71-79页

作者：宋信明王婧茅靳丰任福胜葛秀丽王新广 66469部队北京100042 陆军工程大学国防工程学院江苏南京210007

防护工程内信息化设备散热量大,余热会导致工程内部温度升高、设备失效甚至瘫痪。结合垂直埋管运行高效和水平埋管成本低廉的优势,采用复合埋管式地源热泵系统处理工程内余热以及口部潮湿问题,计算工程内通信机房和办公大厅的全年逐时负... 详细信息

防护工程内信息化设备散热量大,余热会导致工程内部温度升高、设备失效甚至瘫痪。结合垂直埋管运行高效和水平埋管成本低廉的优势,采用复合埋管式地源热泵系统处理工程内余热以及口部潮湿问题,计算工程内通信机房和办公大厅的全年逐时负荷;利用TRNSYS仿真软件构建复合埋管式与垂直埋管式热泵系统模型,比较复合埋管式热泵系统与传统的垂直埋管式热泵系统的热性能、经济效益及防潮效果。结果表明,在工程口部设定水平地埋管可有效降低口部空气相对湿度,起到防潮防湿的作用;在复合埋管式热泵系统总性能系数(coefficient of performance,COP)更高的前提下,复合埋管式热泵系统的总成本较垂直埋管式热泵系统降低了21.8%。

关键词：防护工程复合埋管式热泵系统动态建模数值模拟

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Substrate Stiffness Affected the Inflammatory Response of SMCs

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Journal of Biosciences and Medicines 2021年第3期9卷 44-54页

作者： xiuli mao Lixuan mao Hong Zhang Yiling Tan Huali Wang School of Biomedical Engineering Shanghai Jiao Tong University Shanghai China School of Engineering Computing and Mathematics Plymouth University Plymouth UK

The pathogenesis of atherosclerosis is accompanied by chronic inflammation with changes in the stiffness of the coronary artery wall. Being the main component of the vascular media, the smooth muscle cells (SMCs) are crucial to maintain blood vessel function. SMCs are mechano-sensitive, which can rapidly adapt to the fluctuations in the microenvironment of the blood vessel, including the subtle changes of the vascular stiffness. However, how substrate stiffness influences the phenotype and inflammatory response of SMCs is not well understood. In this study, we investigated the effects of substrate stiffness on SMCs phenotype, inflammatory gene expression and the nuclear factorkappa B (NFκB) signaling pathway of vascular SMCs. From 1 kPa to 100 kPa, the SMCs cytoskeleton became more and more organized with the increase of the substrate stiffness, representing by the uniformed distribution of the stress fibers. SMCs cultured on both soft (1 kPa) and hard (100 kPa) substrate increased the expression of macrophage marker CD68 molecule (CD68) and Galectin 3 (LGALS3) and the inflammatory gene Interleukin-6 (IL-6) and Interleukin-1β (IL-1β) than those on 40 kPa substrate. Moreover, the protein expression level of phosphorylated nuclear factor kappa B inhibitor (p-IκB) was higher on either soft (1 kPa) or hard (100 kPa) substrate. In consistent, the dephosphorylated IκB showed a higher expression level on the substrate stiffness of 40 kPa. These results suggested that substrate stiffness played an important role in SMCs cell morphology, phenotype and inflammatory response by affecting NFκB signaling pathway.

关键词： Vascular Smooth Muscle Cells Substrate Stiffness Inflammatory Response NFκB

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基于药品动态监测的合理用药项目化管理实践

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中华医院管理杂志 2024年第3期40卷 224-229页

作者：杨秀丽石佳娜郑小春严洁萍胡颖叶晓兰毛小红张夙罗丹祁金文黄萍浙江省人民医院(杭州医学院附属人民医院)临床药学中心、药学部杭州310014

药品动态监测是临床合理用药管理的重要抓手。2020年3月,某三级医院建立了药品使用动态监测系统,将药品动态监测结果与药品合理性评价相结合,由医院药事管理与药物治疗委员会下设的工作小组对不合理用药问题突出的异动药品采取合理用药... 详细信息

药品动态监测是临床合理用药管理的重要抓手。2020年3月,某三级医院建立了药品使用动态监测系统,将药品动态监测结果与药品合理性评价相结合,由医院药事管理与药物治疗委员会下设的工作小组对不合理用药问题突出的异动药品采取合理用药项目化管理,逐项推进落实,形成合理用药闭环管理。2020年3月至2022年12月,某医院先后开展了辅助用药、围手术期预防用药和抗菌药物3类药物的合理用药项目化管理实践。实践后,2022年辅助用药收入占比为1.17%,较2019年下降26.88%;骨科质子泵抑制剂预防应激性溃疡的合理率从2019年的53.53%升至2022年的75.12%,围手术期雾化吸入制剂预防用合理率从55.80%升至89.80%;抗菌药物使用强度从2019年的51.30降至2022年的39.28。基于医院药品使用动态监测的合理用药项目化管理能促进临床合理用药,提高工作效率,形成全院"提质、控费、增效"的合理用药局面,可为其他医院加强精细化合理用药管理提供参考。

关键词：药品动态监测项目化管理闭环管理合理用药药学服务

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二氧化硅填料对环氧Vitrimer动态性能的影响

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西南科技大学学报 2024年第1期39卷 25-30页

作者：李燕刘亿司鸿玮宋丽贤周琳赵秀丽陈茂西南科技大学环境友好能源国家重点实验室四川绵阳621010 中国工程物理研究院化工材料研究所四川绵阳621900

将SiO_(2)颗粒引入动态环氧类玻璃高分子(Vitrimer)网络中,制备了具有可控动态性能的环氧Vitrimer。结果表明:填料含量的增加对环氧Vitrimer的基础性能负面影响小,但环氧Vitrimer的特征弛豫时间(τ)、活化能(Ea)、Arrhenius指前因子(τ0... 详细信息

将SiO_(2)颗粒引入动态环氧类玻璃高分子(Vitrimer)网络中,制备了具有可控动态性能的环氧Vitrimer。结果表明:填料含量的增加对环氧Vitrimer的基础性能负面影响小,但环氧Vitrimer的特征弛豫时间(τ)、活化能(Ea)、Arrhenius指前因子(τ0)和拓扑冻结转变温度(Tv)等典型动力学参数都可以很容易地通过填料的含量来调整。SiO_(2)的引入使环氧Vitrimer性能可控,能够按需实现材料的再加工成型。

关键词：环氧类玻璃高分子动态性能调控酯交换反应 SiO_(2)颗粒

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Pharmacokinetic investigation on nteraction between hydrophilic lithospermic acid B and lipophilic tanshinone IIA in rats:an experimental study

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Journal of Traditional Chinese Medicine 2015年第2期35卷 206-210页

作者： Wang xiuli Gao Wei Sun mao School of Chinese Pharmacy Beijing University of Chinese Medicine School of Traditional Chinese Medicine Capital University of Medical Sciences

OBJECTIVE:To elucidate the interaction between hydrophilic lithospermic acid B and lipophilic tanshinone Ⅱ A in rats.METHODS:A reliable high-performance liquid chromatography method was adopted for simultaneous determination of lithospermic acid B and tanshinone Ⅱ A in rat plasma,through which the pharmacokinetic interaction between lithospermic acid B and tanshinone Ⅱ A by intravenous injection was investigated.RESULTS:The simultaneous intravenous injection of tanshinone Ⅱ A and lithospermic acid B significantly altered the pharmacokinetic parameters of both compounds when compared with the individual intravenous administration of each compound.The area under the concentration-time curve of tanshinone Ⅱ A and lithospermic acid B increased by 18.35 and 59.31%,respectively.The mean retention time of tanshinone Ⅱ A and lithospermic acid B increased,respectively,from 9.3 to 32.8 h and20.2 to 49.1 h.The concomitant use of tanshinoneⅡ A magnified the volume of distribution at steady state(V_(ss)) and time for the drug in the plasma to reduce the highest concentration by half(t_(1/2)) of lithospermic acid B,while at the same time the V_(ss) and t_(1/2)of tanshinone Ⅱ A changed significantly in the presence of lithospermic acid B.CONCLUSION:Lithospermic acid B and tanshinone D A interact with each other following simultaneous intravenous injection in rats and this observation may expand the clinical use of Danshen(Radix Salviae Miltiorrhizae).

关键词：动力学相互作用丹参酮ⅡA 丹酚酸B 亲脂性亲水性药代动力学大鼠平均滞留时间

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基于临床和CT影像学特征的胰腺囊性病变进展预测模型的建立及验证

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中华胰腺病杂志 2024年第1期24卷 23-28页

作者：邓文祎谢飞扬毛丽李秀丽孙照勇徐凯朱亮金征宇李骁薛华丹中国医学科学院北京协和医学院、北京协和医院放射科北京100730 深睿医疗人工智能研究院北京100080 南京大学医学院附属金陵医院(东部战区总医院)放射诊断科南京210002

目的构建基于临床及CT影像学特征的胰腺囊性病变(PCLs)进展预测机器学习模型,并在内、外部测试集上评估其预测效能。方法回顾性收集2014年7月至2022年12月间北京协和医院行腹部薄层增强CT扫描的177例患者、200个PCLs病灶的基线临床和影... 详细信息

目的构建基于临床及CT影像学特征的胰腺囊性病变(PCLs)进展预测机器学习模型,并在内、外部测试集上评估其预测效能。方法回顾性收集2014年7月至2022年12月间北京协和医院行腹部薄层增强CT扫描的177例患者、200个PCLs病灶的基线临床和影像学资料,根据随访3年间PCLs病灶是否出现欧洲PCLs研究小组指南规定的手术征象,将其分为进展组和无进展组。以3∶1的比例将200个PCLs病灶随机分为训练集(150个)和内部测试集(50个);以2011年10月至2020年5月间南京大学医学院附属金陵医院行腹部薄层增强CT扫描的14例患者、15个PCLs病灶作为外部测试集。记录患者的临床及CT影像学特征,采用多种特征选择方法、多种机器学习模型进行组合,并基于10折交叉验证法筛选最优机器学习模型。绘制各模型的受试者工作特征曲线(ROC),计算曲线下面积(AUC),取AUC值最高的模型作为最优模型。在测试集上计算AUC值、灵敏度、特异度和准确度,评估模型的预测效能。使用置换重要性评估最优模型特征的重要程度。建立最优模型的校准曲线,采用Hosmer-Lemeshow检验评估模型的临床适用性。结果训练集和内部测试集的进展组与无进展组在胰腺炎史、病灶大小、主胰管管径、主胰管扩张、囊壁增厚、分隔存在、分隔增厚间的差异及内部测试集的两组在性别、病灶钙化和胰腺萎缩间的差异均有统计学意义(P值均<0.05)。外部测试集的进展组与无进展组在病灶大小和胰管扩张间的差异均有统计学意义(P值均<0.05)。基于F检验所选出的胰腺炎史、病灶大小、囊壁增厚、主胰管扩张、主胰管管径5个特征建立的支持向量机模型在交叉验证过程中取得了最高AUC值(0.899)。该模型在内部测试集中对PCLs进展预测的AUC值为0.909,灵敏度为82.4%,特异度为72.7%,准确度为76.0%,在外部测试集中分别为0.944、100%、77.8%和86.7%。校准曲线显示,模型的预测概率与实际PCLs进展概率基本相当。Hosmer-Lemeshow拟合优度检验显示,模型对测试集PCLs进展真实情况的拟合较好。结论基于临床及CT影像学特征构建的支持向量机模型能够帮助医师预测3年内随访PCLs的进展情况,从而帮助临床高效管理患者,合理分配医疗资源。

关键词：胰腺囊性肿瘤机器学习疾病进展计算机体层成像

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An improved method for fluorescence analysis of dissolved organic matter in cave drip water

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Frontiers of Earth Science 2014年第4期8卷 595-598页

作者： xiuli LI Chaoyong HU Jin LIAO Liangliang BAO Qixi mao State key Laboratory of Biogeology and Environmental Geology China University of Geosciences Wuhan 430074 China

为在洞的溶解有机物的决心的一个改进同步 fluorimetric 方法滴水由增加维生素酸，被描述。方法在溶解有机物基于在维生素酸和撤退电子的成分之间的氧化还原作用反应。结果证明增加的维生素酸能熄灭次要的山峰，在 200300 nm，但是不在 ... 详细信息

为在洞的溶解有机物的决心的一个改进同步 fluorimetric 方法滴水由增加维生素酸，被描述。方法在溶解有机物基于在维生素酸和撤退电子的成分之间的氧化还原作用反应。结果证明增加的维生素酸能熄灭次要的山峰，在 200300 nm，但是不在 300500 nm 影响主要山峰的紧张。另外，补充说维生素酸能在一个宽 pH 范围(9-4 ) 上维持相对高、经常的荧光紧张。

关键词：溶解性有机物洞穴滴水荧光分析抗坏血酸氧化还原反应同步荧光法有机物质 pH范围

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Citric acid-assisted synthesis of nano-Ag/BiO Br with enhanced photocatalytic activity

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Science China Chemistry 2015年第3期58卷 457-466页

作者： xiuli Li Xiaoming mao Xiaochao Zhang Yunfang Wang Yawen Wang Hui Zhang Xiaogang Hao Caimei Fan College of Chemistry and Chemical Engineering Taiyuan University of Technology Particle Technology Research Centre Department of Chemical and Biochemical Engineering the University of Western Ontario

In this study, silver nano-particles have been anchored in the surface of Bi OBr photocatalysts by a citric acid-assisted photoreduction method. The citric acid was served as a chelating and reductive agent for the preparation of Ag-decorated Bi OBr photocatalysts(named as Ag/Bi OBr-2). The as-synthesized samples were characterized by X-ray diffraction(XRD), X-ray photoelectron spectroscopy(XPS), scanning electron microscopy(SEM), transmission electron microscopy(TEM), and UV-Vis diffuse reflection spectroscopy(DRS). The Ag/Bi OBr-2 photocatalyst exhibited excellent and stable photocatalytic activities on MO and phenol degradation under simulated sunlight irradiation. The enhanced photocatalytic activity could be ascribed to the smaller size, rough surface, and the surface plasma resonance(SPR) effect of Ag. Also, the Schottky junction, between the surface of the Bi OBr and silver nanoparticles, accelerated the efficient transfer and separation of photoinduced electron-hole pairs and promoted the photocatalytic performance. The active species tests indicated that the superoxide radical(·O-2) was responsible for the enhanced photocatalytic performance of Ag/Bi OBr-2. Finally, a possible photocatalytic mechanism was proposed.

关键词：光催化活性纳米Ag 辅助合成柠檬酸 BIO X-射线光电子能谱银纳米粒子表面等离子体共振

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Namib desert beetle inspired special patterned fabric with programmable and gradient wettability for efficient fog harvesting

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Journal of Materials Science & Technology 2021年第2期61卷 85-92页

作者： Zhihua Yu Huimei Zhang Jianying Huang Shuhui Li Songnan Zhang Yan Cheng Jiajun mao xiuli Dong Shouwei Gao Shanchi Wang Zhong Chen Yaoxing Jiang Yuekun Lai College of Chemical Engineering Fuzhou UniversityFuzhou 350116China National Engineering Laboratory for Modem Silk College of Textile and Clothing EngineeringSoochow UniversitySuzhou 215123China School of Materials Science and Engineering Nanyang Technological University50 Nanyang AvenueSingapore

Efficient collection of water from fog provides a potential solution to solve the global freshwater shortage problem, particularly in the desert or arid regions. In this work, a flexible and highly efficient fog collector was prepared by mimicking the back exoskeleton structure of the Namib desert beetle. The improved fog collector was constructed by a superhydrophobic-superhydrophilic patterned fabric via a simple weaving method, followed by in-situ deposition of copper particles. Compared with the conventional fog collector with a plane structure, the fabric has shown a higher water-harvesting rate at 1432.7 mg/h/cm2,owing to the biomimetic three-dimensional structure, its enhanced condensation performance enabled by the copper coating and the rational distribution of wetting units. The device construction makes use of the widely available textile materials through mature manufacturing technology, which makes it highly suitable for large-scale industrial production.

关键词： Superwettability Fabric Copper Namib desert beetle Fog harvesting

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