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Heterotrimeric G proteinγsubunit DEP1 synergistically regulates grain quality and yield by modulating the TTP(TON1-TRM-PP2A)complex in rice

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Journal of Genetics and Genomics 2023年第7期50卷 528-531页

作者： Yunzhe Wu Ying Zhao Jianping Yu Chenchen Wu Qi Wang Xueying Liu xiuhua gao Kun Wu Xiangdong Fu Qian Liu State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Shandong University of Traditional Chinese Medicine JinanShandong 250355China College of Plant Science and Technology Key Laboratory of New Technology in Agricultural ApplicationBeijing University of AgricultureBeijing 102206China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesCollege of Life SciencesUniversity of Chinese Academy of SciencesBeijing 100101China

Rice is the staple food for nearly half of the world’s population.The negative correlation between rice yield and quality confers breeders a major challenge in how to improve these two traits synergistically.The grain shape,including grain length,grain width,and the ratio of grain length to width,plays an important role in determining rice yield potential and end-use quality.Thus far,some genes determining grain shape and appearance quality,such as GRAIN SIZE 3(GS3)(Fan et al.,2006),GRAIN WIDTH AND WEIGHT 5(GW5)(Liu et al.,2017),GRAIN WIDTH 8(GW8)(Wang et al.,2012),GRAIN SIZE 5(GS5)(Li et al.,2011),GRAIN LENGTH 7/GRAIN WIDTH 7(GL7/GW7)(Wang et al.,2015a,2015b),GRAIN LENGTH AND WEIGHT(GLW7)(Si et al.,2016),GRAIN LENGTH 4(GL4)(Wu et al.,2017),LONG GRAIN AND HIGH YIELD 3(LGY3)(Liu et al.,2018),GRAIN SIZE 9(GS9)(Zhao et al.,2018),GRAIN LENGTH 10(GL10)(Zhan et al.,2022),and BASIC LEUCINE ZIPPER 60(Osb-ZIP60)(Yang et al.,2022),have been cloned and characterized.However,only a few genes have been demonstrated to simultaneously improve grain yield and quality,and the underlying genetic interaction and network also remain largely unknown.

关键词： grain synergistic quality

Relationship between microstructure and hydrogen induced cracking behavior in a low alloy pipeline steel

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Journal of Materials Science & Technology 2017年第12期33卷 1504-1512页

作者： Jing Li xiuhua gao Linxiu Du Zhenguang Liu State Key Laboratory of Rolling and Automation Northeastern University5henyang 110819China

Hydrogen induced cracking（HIC） behaviors of a high strength pipeline steel with three different microstructures, granular bainite ＆ lath bainite（GB ＋ LB）, granular bainite ＆ acicular ferrite（GB ＋ AF）, and quasi-polygonal ferrite（QF）, were studied by using corrosion experiment based on standard NACE TM0284. The HIC experiment was conducted in hydrogen sulfide（H_2S）-saturated solution. The experimental results show that the steel with GB ＋ AF and QF microstructure present excellent corrosion resistance to HIC, whereas the phases of bainite lath and martensite/austenite in LB ＋ GB microstructure are responsible for poor corrosion resistance. Compared with ferrite phase, the bainite microstructure exhibits higher strength and crack susceptibility of HIC. The AF ＋ GB microstructure is believed to have the best combination of mechanical properties and resistance to HIC among the designed steels.

关键词： Pipeline steel Microstructure Hydrogen induced cracking （HIC） Corrosion resistance

Identification of the HAK gene family reveals their critical response to potassium regulation during adventitious root formation in apple rootstock

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Horticultural Plant Journal 2023年第1期9卷 45-59页

作者： Muhammad Mobeen Tahir Lu Tong Lingling Xie Tong Wu Muhammad Imran Ghani Xiaoyun Zhang Shaohuan Li xiuhua gao Leeza Tariq Dong Zhang Yun Shao Yangling Sub-Center of National Center for Apple Improvement College of HorticultureNorthwest Agriculture&Forestry UniversityYanglingShaan 712100China The Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group College of AgricultureShihezi UniversityShiheziXinjiang 832003China Centre of Excellence in Molecular Biology University of the PunjabLahore 53700Pakistan

Adventitious root formation is a bottleneck during vegetative proliferation.Potassium(K^(+))is an essential macronutrient for plants.K^(+)accumulation from the soil and its distribution to the different plant organs is mediated by K^(+)transporters named K^(+)transporter(KT),K^(+)uptake(KUP),or high-affinity K^(+)(HAK).This study aimed to identify members of the HAK gene family in apples and to characterize the effects of K^(+)supply on adventitious root formation and on the expression of HAK genes and the genes that putatively control auxin transport,signaling,and cell fate during adventitious root formation.In this study,34 HAK genes(MdHAKs)were identified in the apple(Malus×domestica‘Golden Delicious’)genome.A phylogenetic analysis divided MdHAKs into four clusters(Ⅰ,Ⅱ,Ⅲ,andⅣ),comprising 16,1,4,and 13 genes,respectively.The syntenic relationships revealed that 62.5%of the total MdHAK genes arise from genomic duplication events.Chromosome location,domain structure,motif analysis,and physico-chemical characteristics were subsequently investigated.Furthermore,the application of K^(+)indicated the emergence of adventitious roots at 8 d and produced more adventitious roots at 16 d than the K^(+)-free control(CK)treatment.In addition,various MdHAKs showed root-specific expression in B9 apple rootstock stem cuttings and enhanced expression during the initiation and emergence stages of adventitious root formation in response to K^(+)treatment.Additionally,K^(+)treatment enhanced the expression levels of MdPIN1,MdPIN2,and MdAUX1.Further data indicated that a higher expression of MdWOX11,MdLBD16,and MdLBD29 and of cell cycle-related genes contributed to the auxin-stimulated adventitious root formation in response to K^(+).

关键词： Apple rootstock Adventitious root Formation Potassium(K+) HAK transporter

Influence of Mass Ratio of Resin and Stabilizer on Mechanical Properties of Mo Fiber-reinforced Granite Polymer Composite

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Journal of Wuhan University of Technology(Materials Science) 2024年第4期39卷 912-920页

作者：张超任秀华 BA Dongzhe ZHANG Jianhua LI Jianyong GUO Mengnan gao Yinghao WANG Guixin LI Jiayang School of Mechanical and Electronic Engineering Shandong Jianzhu UniversityJinan 250101China Key Laboratory of High Efficiency and Clean Mechanical Manufacture Ministry of Education of ChinaSchool of Mechanical EngineeringShandong UniversityJinan 250061China

Because inferior mechanical strength of granite polymer composite(GPC)has become the main drawback limiting its application and popularization,Mo fibers were added into(GPC)to improve its mechanical strength.Mechanical properties of matrix materials with different mass ratio of resin and stabilizer(MRRS)were investigated systematically.The influences of MRRS on interface bonding strength of Mo fiber-matrix,wettability and mechanical strength of GPC were discussed,respectively,and the theoretical calculation result of MRRS k was obtained,with the optimal value of k=4.When k=4,tensile strength,tensile strain and fracture stress of the cured resin achieve the maximum values.But for k=7,the corresponding values reach the minimum.With the increase of MRRS k,surface free energy of the cured resin first increases and then decreases,while contact angles between Mo sample and matrix have displayed the opposite trend.Wettability of resin to Mo fiber is the best at k=4.Pulling load of Mo fiber and interface bonding strength appear the maximum at k=4,followed by k=5,k=3 the third,and k=7 the minimum.When k=4,mechanical properties of Mo fiber-reinforced GPC are optimal,which is consistent with the result of theoretical calculation.This study is of great significance to get better component formulas of Mo fiber reinforced GPC and to improve its application in machine tools.

关键词： polymer composite fiber mechanical strength interface bonding

青贮玉米对湖羊生长性能、瘤胃发酵性能及血清生化指标的影响

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中国饲料 2024年第2期 35-38页

作者：高星梅杜秀花甘肃省康乐县畜牧兽医中心甘肃康乐731500

本研究选择36只体况良好、体重相近的3月龄湖羊为研究对象,随机分为两组,每组3个重复,每个重复6只羊,分别饲喂青贮玉米(试验组)和玉米秸秆(对照组)作为单一粗饲料配制2种日粮,从生长状况、瘤胃发酵情况及血清生化指标等几个方面进行为期... 详细信息

本研究选择36只体况良好、体重相近的3月龄湖羊为研究对象,随机分为两组,每组3个重复,每个重复6只羊,分别饲喂青贮玉米(试验组)和玉米秸秆(对照组)作为单一粗饲料配制2种日粮,从生长状况、瘤胃发酵情况及血清生化指标等几个方面进行为期60d的研究,探讨饲喂青贮玉米对湖羊的影响。结果显示,试验组湖羊的干物质采食量(DMI)和平均日增重(ADG)均显著高于对照组(P 0.05);试验组瘤胃胃液的pH值显著高于对照组8.03%(P 0.05);试验组血清中总蛋白、白蛋白、球蛋白、尿素氮等血清生化指标均无显著差异(P> 0.05)。结果表明,青贮玉米能提高湖羊生长性能,并具有改善湖羊瘤胃发酵的潜力,具有推广应用价值。

关键词：青贮玉米湖羊生长性能瘤胃发酵血清生化指标

Microstructural Evolution and Toughness in the HAZ of Submerged Arc Welded Low Welding Crack Susceptibility Steel

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Acta Metallurgica Sinica(English Letters) 2013年第1期26卷 49-55页

作者： Chunlin QIU Liangyun LAN Dewen ZHAO xiuhua gao Linxiu DU State Key Laboratory of Rolling and Automation Northeastern University

Microstructural characteristics of different sub-regions of heat affected zone （HAZ） of low welding crack susceptibility steel weldment were investigated by using optical microscopy and scanning electron microscopy equipped with electron backscattered diffraction system. And the focus was put on the correlation between microstructural characteristics and HAZ toughness of the weldment. The results reveal that the toughness of fusion line zone （FLZ） specimens is much lower than that of fine grained HAZ （FGHAZ） specimens. The coarse inclusions in the weld metal and the large martensite-austenite constituents in the coarse grained HAZ （CGHAZ） have an obvious negative effect on the crack initiation energy of FLZ. Meanwhile, the coarse granular bainite with large effective grain decreases the crack propagation energy seriously. By contrast, fine crystallographic grains in the FGHAZ play a key role in increasing toughness, especially in improving crack propagation energy.

关键词： Steel Heat affected zone （HAZ） Submerged arc welding Effective grain size Toughness Microstructure

Effects of dietary copper on elemental balance,plasma minerals and serum biochemical parameters of growing-furring male mink(Mustela vison)

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Animal Nutrition 2015年第1期1卷 19-23页

作者： Xuezhuang Wu Hu Cui xiuhua gao Fuhe Yang Institute of Feed Research Chinese Academy of Agricultural Sciences State Key Laboratory for Molecular Biology of Special Economic Animals Institute of Economic Animal and Plant ScienceChinese Academy of Agricultural Sciences

The objectives of this study were to study the effects of different levels of dietary copper on copper and zinc balance, plasma minerals and serum biochemical parameters of mink in the growing-furring periods. One hundred and five standard dark male mink were randomly assigned to seven groups with the following dietary treatments: basal diet with no supplemental Cu(Control); basal diet supplemented with either 6,12, 24, 48, 96, or 192 mg/kg Cu from copper sulfate,respectively. The average daily gain(ADG) linearly(P = 0.0026, P = 0.0006) responded to increasing levels of Cu; maximal growth was seen in the Cu24 group. Feed efficiency tended to improve with the increase of dietary copper level(linear P = 0.0010, quad, P = 0.0011). Fecal copper, urinary copper, retention copper responded in a linear(P < 0.05) fashion with increasing level of Cu. The effect of level of Cu was linear(P < 0.001) for plasma Cu concentration. The serum glutamic-oxalacetic transaminase(GOT) and glutamic-pyruvic transaminase(GPT) activities were increased linearly(P < 0.05) with dose of Cu, but serum total protein(TP) and albumin(ALB) concentrations decreased linearly(P < 0.05) as dietary copper levels increased. Effect of level of Cu was linear(P < 0.001) for serum ceruloplasmin(CER) concentration or Cu-Zn superoxide dismutase(Cu-Zn SOD) activity. Supplemental dose of Cu linearly decreased serum triglyceride(TG)(P = 0.011) and total cholesterol(TC)(P = 0.007). Our results indicated that the activity of Cu-dependent enzymes was enhanced by increasing dietary Cu concentration and that supplementation of Cu in the diet of mink could alter the plasma lipid profile and copper concentration.

关键词： Mink Copper Plasma minerals Serum biochemical parameters

Primary Recrystallization of Grain Oriented Silicon Steel Strip Rolled by CSR and Annealed in Magnetic Field

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Journal of Materials Science & Technology 2005年第4期21卷 455-458页

作者： En LIU Kemin QI xiuhua gao Chunlin QIU Hezhou YE State Key Laboratory of Rolling and Automation Northeastern University Shenyang 110004 China

The magnetic properties and textures of grain oriented silicon steel with different thickness rolled by cross shear rolling （CSR） of different mismatched speed ratio （MSR） and annealed in magnetic field under hydrogen were presented.Effects of the factors such as thickness and mismatched speed ratio on the magnetic properties and recrystallization texture were analyzed and the recrystallization principles in magnetic field annealing were discussed. The study would provide a new route for mass production of high quality ultra-thin grain oriented silicon steel strip.

关键词： Primary recrystallization Grain oriented silicon steel Cross shear rolling Magnetic field annealing

Effect of boron addition on the microstructures and mechanical properties of thermomechanically processed and tempered low carbon bainitic steels

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Acta Metallurgica Sinica(English Letters) 2011年第6期24卷 473-486页

作者： Liangyun LAN Chunlin QIU Ping ZHOU Dewen ZHAO Canming LI xiuhua gao Linxiu DU The State Key Laboratory of Rolling and Automation Northeastern University Shenyang 110819 China Technical Research and Development Center Laiwu Steel Group Laiwu 271104 China

Thermomechanical process and tempering heat treatment were employed to produce the experimental steel plates. The effect of boron addition on the microstructure and mechanical properties of low carbon bainitic steels was studied in this paper. Microstructure observation and crystallographic features were conducted by using optical microscopy, SEM, TEM and electron back scattering diffraction （EBSD） analysis. The results showed that under the same rolling processes and heat treatment conditions, a substantial increase in strength is obtained by addition of boron into steel, but accompanied by an obvious drop in toughness. New martensite phase forms along the grain boundaries on tempering at 650℃ mainly due to boron segregation, which can further deteriorate impact toughness of the boron bearing steel. The EBSD analysis showed that high angle grain boundary, is not responsible for the deteriorated toughness of the boron bearing steel because it has relatively higher percentage of high angle grain boundary than the boron free steel. The low toughness of the boron bearing steel is mainly attributed to the coarse boride precipitated particles according to the results of fractograph observation.

关键词： Low carbon bainitic steel Boron Tempering Microstructure Brittle fracture