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河北师范大学学报（自然科学版） 2017年第6期41卷 521-527页

作者：李琴殷妍车飞王菲菲张艳平马妍赵秀阁中国环境科学研究院环境基准与风险评估国家重点实验室北京100012 中国环境科学研究院环境标准研究所北京100012 首都儿科研究所儿童早期综合发展研究室北京100020 中国疾病预防控制中心营养与健康所北京100050

介绍了环境中砷的测定方法,包括目前比较成熟的用于砷测定的光度分析法和原子光谱法中的各种技术,以及处于研究阶段的生物学方法、电化学方法及用于砷的形态分析的联用技术,并结合目前砷环境监测分析方法标准的现状,展望了环境中砷的测... 详细信息

介绍了环境中砷的测定方法,包括目前比较成熟的用于砷测定的光度分析法和原子光谱法中的各种技术,以及处于研究阶段的生物学方法、电化学方法及用于砷的形态分析的联用技术,并结合目前砷环境监测分析方法标准的现状,展望了环境中砷的测定方法的发展方向.

关键词：砷环境标准分析方法

Long noncoding RNAs:new insights in modulating mammalian spermatogenesis

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Journal of Animal Science and Biotechnology 2020年第2期11卷 291-302页

作者： Bahlibi Weldegebriall Sahlu Shanjiang zhao xiuge Wang Saqib Umer Huiying Zou Jinming Huang Huabin Zhu Embryo Biotechnology and Reproduction Laboratory Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193People’s Republic of China Tigray Agricultural Research Institute Mekelle Agricultural Research CenterMekelleEthiopia Dairy Cattle Research Center Shandong Academy of Agricultural SciencesJinan 250131People’s Republic of China

Spermatogenesis is a complex differentiating developmental process in which undifferentiated spermatogonial germ cells differentiate into spermatocytes,spermatids,and finally,to mature *** multistage developmental process of spermatogenesis involves the expression of many male germ cell-specific long noncoding RNAs(lncRNAs)and highly regulated and specific gene *** are a recently discovered large class of noncoding cellular transcripts that are still relatively *** a few of them have postmeiotic;however,lncRNAs are involved in many cellular biological *** expression of lncRNAs is biologically relevant in the highly dynamic and complex program of spermatogenesis and has become a research focus in recent genome *** review considers the important roles and novel regulatory functions whereby lncRNAs modulate mammalian spermatogenesis.

关键词： Germ cell Long noncoding RNA Mammalian spermatogenesis Regulatory pathways Spermatocytes

CCDC178-mediated cytoskeleton assembly is required for spermiogenesis in mice

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Genes & Diseases 2024年第6期11卷 56-59页

作者： Fucheng Dong xiuge Wangg Tao Huangg Yingying Yin Sai Xiao Yanjie Ma Huafang Wei Bingbing Wu Ruidan Zhang Liying Wang Xuejiang Guo Fei Gao Chao Liu Hongbin Liu Jianguo zhao Wei Li State Key Laboratory of Stem Cell and Reproductive Biology Instituteof ZoologyStem Cell and Regenerative Medicine Innovation InstituteChinese Academy of SciencesBeijing 100101China Guangzhou Women and Children's Medical Center Guangzhou Medical UniversityGuangzhouGuangdong 510623China Shandong Technology Innovation Center for Reproductive Health JinanShandong 250012China Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural SciencesJinanShandong 250199China Key Laboratory of Reproductive Endocrinologyof Ministry of Education Shandong UniversityJinanShandong 250012China Shandong Provincial Clinical Medicine Research Center for ReproductiveHealth JinanShandong 250012China State Key Laboratory of Reproductive Medicine Nanjing Medical University NanjingJiangsu 211166China University of Chinese Academy of Sciences Beijing 100049China

Infertility affects around 8%-12%of couples globally,and in about 50%of these cases,male factors are either the primary cause or contribute significantly to *** defects during spermiogenesis may result in male subfertility or complete infertility in mammals.1 Previously,we found that CFAP53 is localized in the manchette and sperm tail,and it plays an essential role in sperm flagellum biogenesis.

关键词： infertility globally cases

Analysis of Vagina Microecology and Investigation of Common Infection in Border Area of Chongzuo City

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Advances in Reproductive Sciences 2022年第1期10卷 1-11页

作者： Huan zhao Yan Chen Liuxian Huang Tianyou Huang Miaohong Li Wanwei Yang Qing Peng Lili Yu Haisha Wei Jianlan Gan xiuge Li The Clinical Laboratory of Chongzuo Maternal and Child Health Care Hospital Chongzuo China

Objective: To investigate the microecology and common infection of the female vagina in border area of Chongzuo City. Methods: Female patients in the outpatient department and inpatient department of gynecology of our hospital from April 1, 2018 to August 23, 2021 were selected as the research objects. The reproductive tract secretions of all patients were examined by microecological examination, morphological examination, chemical analysis and microscopic examination, and the results of various indicators were statistically analyzed to draw relevant conclusions. Results: Among the 1498 women with vaginal micro- ecological imbalances, cleanliness was determined to be 534 cases of degree II, 868 cases of degree III, and 96 cases of degree IV;Nugent scores were 9 - 10 in 9 cases, 8 in 122 cases, 7 in 30 cases, 6 in 18 cases, 5 in 8 cases, 4 in 717 cases, 3 in 248 cases, 2 in 209 cases, 1 in 118 cases, and 0 in 19 cases. AV scores were 8 in 1 case, 7 in 1 case, 6 in 2 cases, 5 in 9 cases, 4 in 84 cases, 3 in 207 cases, 2 in 850 cases, 1 in 284 cases, and 0 in 60 cases. Among the pathogen infections, 99 cases were mycoplasma, 361 cases were candida, 199 cases were bacterial and 8 cases were trichomonas;In chemical analysis, there were 138 cases with pH 5.1, 326 cases with pH 4.8, 775 cases with pH 4.6, 217 cases with pH 4.4, and 42 cases with pH 4.1;meanwhile, 1390 cases were positive for hydrogen peroxide, 505 cases were positive for glucosidase, 487 cases were positive for acetylglucosaminidase, 184 cases were positive for sialidase, 290 cases were positive for proline amino-peptidase, 501 cases were positive for coagulase, and 1064 cases were positive for leukocyte esterase. Conclusion: Female reproductive tract microecology is more complex, and the pathogens of infection are also diverse. A systematic examination can clarify the occurrence and development of the disease, and then provide reliable laboratory data for clinical treatment and guide clinical medication.

关键词： Border Area Chongzuo City Female Reproductive Tract Microecology Infection

Investigation on the Distribution of Common Thalassemias in Various Cities and Counties under the Jurisdiction of Chongzuo City, Guangxi

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Advances in Bioscience and Biotechnology 2021年第11期12卷 361-370页

作者： Huan zhao Yan Chen Jing Lan Liuxian Huang Tianyou Huang Miaohong Li Xingchuang Chen Wanwei Yang Fu Huang Tongfeng Huang xiuge Li Inspection Department of Chongzuo Maternal and Child Health Care Hospital Chongzuo China

Objective: To understand the distribution of thalassemia in all districts under the jurisdiction of Chongzuo City. Methods: Collect blood routine indicators from May 2014 to 31 December 2020 in the districts of this city to screen out suspected Mediterranean patients for genetic diagnosis;GGAP-PCR and PCR-reverse dot hybridization were used to analyze the thalassemia gene in the specimens of suspected patients;compare the prevalence of thalassemia in the counties and cities within the jurisdiction of this city, and provide corresponding medical advice to the health authorities. Results: 21,535 venous blood specimens from patients with suspected thalassemia were collected in the city. There were 14,215 positive cases of thalassemia, accounting for 66.01% of the total number of patients, among which 9455 cases (43.91%) were pure α gene positive. 3464 patients (16.09%) were positive for simple β gene. 1296 patients (6.02%) were positive for αβ double gene. The proportions of thalassemia gene testing for α-thalassaemia gene, β-thalassaemia gene, and α-β double gene in various counties and districts were different. According to the multiple rate or the chi-square test of the constituent ratio, the comparison of the distribution of the thalassaemia gene test results in each area, χ2 = 472.6917, P = 0.0000, the difference is statistically significant. Conclusion: Severe thalassemia is a tragedy for a family. It not only needs to spend a lot of money to prolong life, but it also cannot change the situation of losing life and financial emptiness in the end. It is suggested that timely screening, timely diagnosis and medical consultation should be carried out in married and unborn families and early pregnancy, so as to reduce the birth of children with severe thalassemia and avoid the occurrence

关键词： Thalassemia α-Thalassaemia Gene β-Thalassaemia Gene Distribution Status Gene Analysis

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紫外光固化环氧丙烯酸酯涂料的制备研究

化工新型材料 2010年第7期38卷 123-125页

作者：陈奎曹秀鸽王玲赵宇洁兰州城市学院培黎工程技术学院兰州730070 浙江大学材料科学与化学工程学院杭州310027

为解决环氧丙烯酸酯黏度较大这一问题,以低黏度的γ-缩水甘油醚氧丙基三甲氧基硅烷(A187)代替环氧树脂,讨论了环氧丙烯酸酯合成中A187与丙烯酸的配比、催化剂、反应温度和阻聚剂对反应的影响,研究了光引发剂含量对UV涂膜性能的影响。确... 详细信息

为解决环氧丙烯酸酯黏度较大这一问题,以低黏度的γ-缩水甘油醚氧丙基三甲氧基硅烷(A187)代替环氧树脂,讨论了环氧丙烯酸酯合成中A187与丙烯酸的配比、催化剂、反应温度和阻聚剂对反应的影响,研究了光引发剂含量对UV涂膜性能的影响。确定了该紫外光固化涂料的工艺。

关键词：环氧丙烯酸酯紫外光固化涂料

基于AMPK/mTOR/ULK1通路探讨解毒通络保肾方改善糖尿病肾脏疾病的作用机制

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北京中医药大学学报 2022年第12期45卷 1213-1222页

作者：王秀阁闫冠池金迪赵芸芸于淼长春中医药大学附属医院内分泌代谢病科长春130021 长春中医药大学

目的通过动物实验及细胞实验观察解毒通络保肾方对糖尿病肾脏疾病(DKD)大鼠肾脏病理形态及大鼠肾小球系膜细胞(HBZY-1)增殖的影响,探讨其改善DKD的作用机制。方法(1)从85只健康雄性SD大鼠中任取10只纳入正常组,其余大鼠采用高脂饲料喂... 详细信息

目的通过动物实验及细胞实验观察解毒通络保肾方对糖尿病肾脏疾病(DKD)大鼠肾脏病理形态及大鼠肾小球系膜细胞(HBZY-1)增殖的影响,探讨其改善DKD的作用机制。方法(1)从85只健康雄性SD大鼠中任取10只纳入正常组,其余大鼠采用高脂饲料喂养与链脲佐菌素注射诱导为DKD模型。造模成功的大鼠按体质量随机分为模型组,厄贝沙坦组(16 mg/kg),解毒通络保肾方低、中、高剂量组(0.5、1.0、2.0 g/kg)。正常组及模型组给予等量生理盐水灌胃。每日1次,连续灌胃8周。采用光学显微镜和透射电镜观察肾脏组织病理形态,蛋白质印迹法检测肾脏组织自噬关键分子酵母Atg6同系物(Beclin-1)、自噬蛋白微管相关蛋白1轻链3(LC3Ⅱ/LC3Ⅰ)、泛素结合蛋白P62(P62)、自噬相关蛋白5(ATG5)的表达。(2)培养HBZY-1细胞,分为空白对照组(5.5 mmol/L葡萄糖),模型组(30 mmol/L葡萄糖),解毒通络保肾方低、中、高剂量组(50、100、200 mg/L),厄贝沙坦组(5μmol/L)。采用CCK8法检测细胞增殖率,划痕实验检测细胞迁移能力,蛋白质印迹法检测LC3Ⅱ/LC3Ⅰ、P62、ATG5与腺苷酸活化蛋白激酶(AMPK)、哺乳动物雷帕霉素靶蛋白(mTOR)、unc-51样激酶1(ULK1)及磷酸化蛋白(p-ULK1、p-AMPK及p-mTOR)的表达。结果(1)解毒通络保肾方可改善DKD大鼠肾脏基底膜、系膜、肾小管上皮细胞病理形态与超微结构,增加自噬小体、自噬溶酶体数量。与正常组比较,模型组Beclin-1、LC3Ⅱ/LC3Ⅰ、ATG5蛋白表达降低,P62蛋白表达增加(P<0.01);解毒通络保肾方干预后,肾组织中Beclin-1、LC3Ⅱ/LC3Ⅰ、ATG5蛋白表达增加,P62蛋白表达降低(P<0.05,P<0.01)。(2)解毒通络保肾方可抑制高糖诱导的HBZY-1细胞增殖,增加LC3Ⅱ/LC3Ⅰ、ATG5、p-ULK1、p-AMPK蛋白表达,降低P62、p-mTOR蛋白表达(P<0.05,P<0.01)。结论解毒通络保肾方可改善DKD大鼠肾脏组织病理与超微结构,抑制高糖诱导的HBZY-1细胞增殖,其机制可能是通过调控AMPK/mTOR/ULK1通路,改善细胞自噬实现的。

关键词：解毒通络保肾方糖尿病肾脏疾病腺苷酸活化蛋白激酶/哺乳动物雷帕霉素靶蛋白/unc-51样激酶1通路肾系膜细胞自噬大鼠

4个品种核桃砧木幼苗干旱生理响应及抗旱性评价

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经济林研究 2020年第1期38卷 11-19页

作者：刘丙花赵登超梁静舒秀阁贾明王小芳山东省林业科学研究院山东济南250014

【目的】对4个品种核桃砧木幼苗进行抗旱性评价,旨在筛选适合干旱、半干旱核桃产区的优良抗旱核桃砧木。【方法】以4个常用核桃砧木品种为试材,研究干旱对其盆栽幼苗生长速率、水分利用效率、光合参数和叶绿素荧光参数的影响,采用模糊... 详细信息

【目的】对4个品种核桃砧木幼苗进行抗旱性评价,旨在筛选适合干旱、半干旱核桃产区的优良抗旱核桃砧木。【方法】以4个常用核桃砧木品种为试材,研究干旱对其盆栽幼苗生长速率、水分利用效率、光合参数和叶绿素荧光参数的影响,采用模糊数学隶属函数法对其抗旱性进行综合评价。【结果】干旱条件下,核桃砧木幼苗间存在显著的品种间差异,其中,‘鸡爪绵’的相对生长速率(R)、长期水分利用效率(R_(LWUE))、净光合速率(P_n)和光系统Ⅱ的最大光化学效率(F_v/F_m)最高,分别为0.92 mg/d、70.24 g/L、15.22 μmol/(m^2·s)和0.79。干旱显著降低了核桃砧木幼苗的R、P_n和F_v/F_m,其中‘鸡爪绵’的降低幅度最小,分别为30.30%、22.78%和7.06%。干旱显著提高了核桃砧木幼苗的R_(LWUE)和瞬时水分利用效率(R_(IWUE))。‘核桃楸’的R_(LWUE)增幅最大,为16.20%,其次是‘鸡爪绵’,为15.70%。‘鸡爪绵’的R_(IWUE)增幅最大,为16.61%。‘鸡爪绵’‘香玲’‘核桃楸’和‘黑核桃’的隶属函数平均值分别为0.99、0.68、0.48和0.07,表明核桃砧木幼苗抗旱性存在较大的品种间差异。【结论】‘鸡爪绵’和‘香玲’抗旱性强,且种子易获得,适合在干旱半干旱地区广泛应用,‘黑核桃’和‘核桃楸’抗旱性弱,且种子较难获得,不适宜在干旱地区应用。

关键词：核桃砧木干旱胁迫抗旱性隶属函数评价法

不同树形对早实核桃‘鲁光’坚果产量和品质的影响

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果树学报 2021年第1期38卷 73-81页

作者：刘丙花唐贵敏梁静舒秀阁李萍萍赵登超侯立群山东省林业科学研究院济南250014 山东英才学院济南251400 山东汇友核桃研究院山东章丘250200

【目的】探索适宜‘鲁光’核桃优质高产的树形。【方法】以‘鲁光’核桃树为研究对象,分析主干分层形、开心形和自然圆头形3种树形的结构参数、坚果产量和品质,并进行主成分分析。【结果】主干分层形的树高、基径、骨干枝数、单株平均... 详细信息

【目的】探索适宜‘鲁光’核桃优质高产的树形。【方法】以‘鲁光’核桃树为研究对象,分析主干分层形、开心形和自然圆头形3种树形的结构参数、坚果产量和品质,并进行主成分分析。【结果】主干分层形的树高、基径、骨干枝数、单株平均坚果产量、树冠投影面积平均产量、单果质量、仁质量、种仁粗脂肪和抗坏血酸含量最高;开心形的坚果壳厚最小,出仁率和种仁蛋白质含量最高;自然圆头形的总糖和氨基酸含量最高。坚果外观形状无显著的树形间差异。主成分分析表明主干分层形有利于产量的形成,开心形有利于品质的形成,3种树形的综合排名顺序为主干分层形>开心形>自然圆头形。【结论】济南市章丘区‘鲁光’核桃的适宜树形为主干分层形。

关键词：核桃树形产量品质主成分分析