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LncRNA CACNA1G-AS1 up-regulates FTH1 to inhibit ferroptosis and promote malignant phenotypes in ovarian cancer cells

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Oncology Research 2023年第2期31卷 169-179页

作者： YANPING JIN JIANPING QIU xiufang lu YAN MA GUOWEI LI Department of Obstetrics and Gynecology The Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou Municipal Hospital NorthSuzhouChina Department of Neurosurgery The Second Affiliated Hospital of Soochow UniversitySuzhouChina

Previous study revealed that ferritin heavy chain-1(FTH1)could regulate ferritinophagy and affect intracellular Fe^(+)content in various tumors,while its N6-methyladenosine(m6A)RNA methylation was closely related the prognosis of ovarian cancer ***,little is known about the role of FTH1 m6A methylation in ovarian cancer(OC)and its possible action *** this study we constructed FTH1 m6A methylation regulatory pathway(LncRNA CACNA1G-AS1/IGF2BP1)according to related bioinformatics analysis and research,through clinical sample detections we found that these pathway regulatory factors were significantly up-regulated in ovarian cancer tissues,and their expression levels were closely related to the malignant phenotype of ovarian *** vitro cell experiments showed that LncRNA CACNA1G-AS1 could up-regulate FTH1 expression through IGF2BP1 axis,thus inhibited ferroptosis by regulating ferritinophagy,and finally promoted proliferation and migration in ovarian cancer ***-bearing mice studies showed that the knock-down of LncRNA CACNA1G-AS1 could inhibited the tumorigenesis of ovarian cancer cells in vivo *** results demonstrated that LncRNA CACNA1G-AS1 could promote the malignant phenotypes of ovarian cancer cells through FTH1-IGF2BP1 regulated ferroptosis.

关键词： Ovarian cancer m6A methylation Ferroptosis Mitophagy Malignant phenotype

尿路感染患者耐碳青霉烯类肠杆菌目细菌耐药基因特征分析

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中华全科医学 2024年第10期22卷 1684-1686,1773页

作者：路秀芳康亚丽李霞胡金鼎田金静聊城市第二人民医院检验科山东聊城252600

目的探讨尿路感染患者耐碳青霉烯类肠杆菌目细菌(CRE)的耐药性及耐药基因特征,为本地区CRE防治提供依据。方法收集2017年1月—2022年12月聊城市第二人民医院尿路感染患者细菌培养分离的81株CRE的临床资料,分析其耐药性及耐药基因特征。... 详细信息

目的探讨尿路感染患者耐碳青霉烯类肠杆菌目细菌(CRE)的耐药性及耐药基因特征,为本地区CRE防治提供依据。方法收集2017年1月—2022年12月聊城市第二人民医院尿路感染患者细菌培养分离的81株CRE的临床资料,分析其耐药性及耐药基因特征。结果81株CRE中,大肠埃希菌占比55.56%(45/81),肺炎克雷伯菌占比28.40%(23/81)。CRE对多黏菌素、替加环素呈现较低耐药率[4.94%(4/81)、0];大肠埃希菌对头孢他啶/阿维巴坦耐药率高达84.44%(38/45),而肺炎克雷伯菌对其呈现较低耐药率[8.70%(2/23)]。81株CRE中分型为blaKPC 22株,均为blaKPC-2;blaNDM 59株,其中57株blaNDM-5,2株blaNDM-1。大肠埃希菌的耐药基因主要为blaNDM-5型[97.78%(44/45)],肺炎克雷伯菌的耐药基因主要为blaKPC-2型[95.65%(22/23)]。23株碳青霉烯耐药肺炎克雷伯菌(CRKP)ST型以ST11为主[86.96%(20/23)],45株碳青霉烯耐药大肠埃希菌(CREC)ST型以ST410为主[51.11%(23/45)]。结论尿路感染患者CRE对多种抗菌药物耐药率较高,大肠埃希菌的耐药基因主要为blaNDM-5型,blaKPC-2型为肺炎克雷伯菌的主要耐药基因,ST分型主要为ST11型。

关键词：尿路感染肠杆菌目碳青霉烯类耐药基因药敏分析

1T′-MoTe_(2)-Based On-Chip Electrocatalytic Microdevice:A Platform to Unravel Oxidation-Dependent Electrocatalysis

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CCS Chemistry 2019年第5期1卷 396-406页

作者： Hui You Zhiwen Zhuo xiufang lu Youwen Liu Yabin Guo Wenbin Wang Huan Yang Xiaojun Wu Huiqiao Li Tianyou Zhai State Key Laboratory of Material Processing and Die&Mould Technology and School of Materials Science and EngineeringWuhan National High Magnetic Field CenterHuazhong University of Science and TechnologyWuhanHubei 430074China CAS Key Laboratory of Materials for Energy Conversion and Department of Material Science and Engineering Hefei National Laboratory of Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefeiAnhui 230026China

Ultrathin transition metal dichalcogenides(TMDs)are of particular interest as low-cost alternatives to highly active electrocatalysts because of their high surface activation ***,their striking structural characterist... 详细信息

关键词： 1T′-MoTe_(2) on-chip electrocatalytic micro-device electron density modulator catalytic model hydrogen evolution reaction

Long-term(2013-2022)mapping of winter wheat in the North China Plain using Landsat data:classification with optimal zoning strategy

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Big Earth Data 2024年第3期8卷 494-521页

作者： Yifei Liu Xuehong Chen Jin Chen Yunze Zang Jingyi Wang Miao lu Liang Sun Qi Dong Bingwen Qiu xiufang Zhu State Key Laboratory of Remote Sensing Science Institute of Remote Sensing Science and EngineeringFaculty of Geographical ScienceBeijing Normal UniversityBeijingChina Beijing Engineering Research Center for Global Land Remote Sensing Products Institute of Remote Sensing Science and EngineeringFaculty of Geographical ScienceBeijing Normal UniversityBeijingChina State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural Sciences(CAAS)BeijingChina State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijingChina Key Laboratory of Spatial Data Mining&Information Sharing of Ministry of Education Academy of Digital China(Fujian)Fuzhou UniversityFuzhouChina

security and formulating agricultural *** data are the only available source for long-term winter wheat mapping in the North China Plain due to the fragmented landscape in this *** various methods,such as index-based methods,curve similarity-based methods and machine learning-based methods,have been developed for winter wheat mapping based on remote sensing,the former two often require satellite data with high temporal resolution,which are unsuitable for Landsat data with sparse *** learning is an effective method for crop classification using Landsat ***,applying machine learn-ing for winter wheat mapping in the North China Plain encounters two main issues:1)the lack of adequate and accurate samples for classifier training;and 2)the difficulty of training a single classifier to accomplish the large-scale crop mapping due to the high spatial heterogeneity in this *** address these two issues,we first designed a sample selection rule to build a large sample set based on several existing crop maps derived from recent Sentinel data,with specific consideration of the confusion error between winter wheat and winter rapeseed in the available crop ***,we developed an optimal zoning method based on the quadtree region splitting algorithm with classification feature consistency criterion,which divided the study area into six subzones with uni-form classification *** each subzone,a specific random forest classifier was trained and used to generate annual winter wheat maps from 2013 to 2022 using Landsat 8 OLI *** sample validation confirmed the high accuracy of the produced maps,with an average overall accuracy of 91.1%and an average kappa coefficient of 0.810 across different *** derived winter wheat area also has a good correlation(R2=0.949)with census area at the provincial *** results underscore the reliability of the produced annual winter wheat *** experiments demonstrate that

关键词： Winter wheat mapping Landsat machine learning North China Plain optimal zoning

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胶质淋巴系统在帕金森病中的研究进展

中华神经科杂志 2022年第12期55卷 1434-1441页

作者：王韬卢秀芳王荔山西医科大学第二医院神经内科山西医科大学第二临床医学院太原030001 山西医科大学第二医院神经内科太原030001

帕金森病是脑内异常蛋白沉积为主要病理改变的中枢神经系统退行性疾病,目前认为与异常沉积蛋白清除障碍有关,至今相关机制仍不明确。胶质淋巴系统是脑内代谢废物及异常蛋白沉积清除系统,近年来研究结果表明,帕金森病中水通道蛋白4、血... 详细信息

帕金森病是脑内异常蛋白沉积为主要病理改变的中枢神经系统退行性疾病,目前认为与异常沉积蛋白清除障碍有关,至今相关机制仍不明确。胶质淋巴系统是脑内代谢废物及异常蛋白沉积清除系统,近年来研究结果表明,帕金森病中水通道蛋白4、血管周围间隙及硬脑膜淋巴管改变与脑脊液中相关病理蛋白的水平密切相关,最终导致帕金森病的发生与发展。文中对近年来帕金森病中胶质淋巴系统结构及生物标志物研究进行综述。

关键词：帕金森病水通道蛋白质4 α突触核蛋白淀粉样蛋白 tau蛋白质类胶质淋巴系统血管周围间隙

A Simplified PCR-SSP Method for HLA-A2 Subtype in a Population of Wuhan,China

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Cellular & Molecular Immunology 2006年第6期3卷 453-458页

作者： Bing Liang Lijun Zhu Zhihui Liang xiufang Weng Xiaoling lu Cai＇e Zhang Hui Li Xiongwen Wu Department of Immunology Tongjl Medical College Huazhong University of Science ＆ Technology Wuhan China

HLA-A2 is the most frequent HLA-A allele in all ethnic populations, and an important restriction element for peptide presentation to T cells in infectious disease and cancer. However, the HLA-A2 supertype consisting of up to 75 subtypes, mutation studies and analyses using cytotoxic T lymphocytes suggest the functional relevance of subtype-specific differences in HLA-A2 molecules for peptide binding and T-cell recognition. Therefore, it is necessary for T-cell response study to discriminate the HLA-A2 subtypes and to understand the profile of HLA-A2 allellc distribution in a given population. In this study, we developed a simple, robust approach based on the nested polymerase chain reaction using sequence-specific primers （PCR-SSP） to discriminate 17 HLA-A2 subtypes which cover the most HLA-A2 alleles （〉 99% allele frequency） reported in Chinese, using 15 combinations of 19 allelic specific primers. In the first round of PCR, 3 combinations of 5 primers were used to determine whether the tested sample was HLA-A2 positive, meanwhile the subtypes of HLA-A＊0209 and HLA-A＊0215N were determined for the variant position of these two subtypes is in exon 4 instead of exon 2, 3. Samples of HLA-A2 positive were subtyped in the second round of PCR, using PCR products of the first round as templates. This strategy was applied to test the samples of 78 random HLA-A2 positive individuals for their HLA-A2 subtypes. Those samples were screened for HLA-A2 positive by the first round PCR-SSP from 154 healthy blood donors in Wuhan, China. The subtyping results were verified by using flow cytometric analysis （FCM） with HLA-A2 specific monoclonal antibody BB7.2 and DNA sequencing. The typing results of the samples show 50.7% random individuals in the population carry HLA-A2, HLA-A＊0201 ranks the first （allele frequency = 15.5%）, followed by A＊0207 （5.8%）, A＊0206 （4.7%）, A＊0203 （2.6%）, A＊0210 （0.7%）, and these 5 alleles account for 99.0% HLA-A2 subtypes of allele frequency. Our study ind

关键词： HLA-A2 PCR-SSP subtype

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基于利率情景生成的寿险公司经济资本量化

保险研究 2017年第6期 15-26页

作者：李秀芳卢山南开大学金融学院天津300350

本文构建了符合中国风险导向偿付能力体系(简称"C-ROSS")监管原则的寿险公司经济资本模型,通过MATLAB编程实现利率情景生成和寿险公司自下而上的经济资本量化,并对保险风险经济资本、利率风险经济资本和整体风险经济资本展开分析,探讨... 详细信息

本文构建了符合中国风险导向偿付能力体系(简称"C-ROSS")监管原则的寿险公司经济资本模型,通过MATLAB编程实现利率情景生成和寿险公司自下而上的经济资本量化,并对保险风险经济资本、利率风险经济资本和整体风险经济资本展开分析,探讨了风险聚合过程中的经济资本分散化效应以及风险内聚合与风险外聚合对经济资本的影响。研究结果显示:保险风险和利率风险聚合的风险分散化效应较为稳定,但对于长寿和死亡这两个互斥风险,通过相关系数矩阵进行聚合的实质是假设了死亡率改善和恶化的概率,并且该概率会随着年龄的改变而变化;风险内聚合的经济资本显著大于风险外聚合的经济资本,风险外聚合所带来的风险分散化效应对于女性保单最为明显;从降低公司整体经济资本占用出发,寿险公司应该更多地向男性客户销售终身寿险。

关键词：经济资本利率情景生成寿险公司

Study on correlation between C-reactive protein and gestational diabetes mellitus

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Journal of Nanjing Medical University 2007年第6期21卷 382-385页

作者： Xiaohong Li xiufang lu Department of Obstetrics and Gynecology North District of the Affiliated Suzhou Hospital of Nanjing Medical University Suzhou 215008 Jiangsu Province China

Objective：To investigate correlation between C-reactive protein（CRP） and gestational diabetes mellitus（GDM）, Methods：Twentyfive GDM women were served as study group, and thirty normal pregnant women were selected as control group. The serum FPG, 2hPG, HbAlc and CRP levels and the leukocyte count were detected in the two groups, in order to observe the relationship between gestational diabetes mellitus and inflammatory markers. Results：The age and gestational week did not show difference in the two groups（P〉 0.05）. But there was a significant difference in body mass index（BMI） between the GDM group and the controtgroup（P 〈 0.05）. The serum FPG, 2hPG, HbAlc and CRP levels and the leukocyte count in the GDM group were higher than those in the control group, and the difference was significant（P 〈 0.05）, There was positive correlation between serum C-reactive protein value and FPG, 2hPG, HbAlc serum levels or the leukocyte count in GDM group. But in the control group there was no correlation between them. Conclusion：The results suggest that there is correlation between C-reactive protein and gestational diabetes mellitus, and inflammation may play an important role in the development of gestational diabetes mellitus.

关键词： C-reactive protein diabetes gestational inflammation

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探讨小学语文教学中德育渗透的实践

新课程学习(下) 2014年第8期 180-181页

作者：吕秀芳大通县第四完全中学

小学阶段,由于小学生的知识、年龄以及经验等的欠缺,德育教育的开展需要结合实际,运用适宜的教育方式。主要探讨了阅读与写作途径中的德育渗透。

关键词：语文德育渗透实践