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Antibodies elicited by Newcastle disease virus-vectored H7N9 avian influenza vaccine are functional in activating the complement system

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Journal of Integrative Agriculture 2024年第6期23卷 2052-2064页

作者： Zenglei Hu Ya Huang Jiao Hu Xiaoquan Wang Shunlin Hu xiufan liu Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China Yangzhou UniversityYangzhou 225009China Animal Infectious Disease Laboratory School of Veterinary MedicineYangzhou UniversityYangzhou 225009China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhou 225009China

H7N9 subtype avian influenza virus poses a great challenge for poultry *** disease virus(NDV)-vectored H7N9 avian influenza vaccines(NDV_(vec)H7N9)are effective in disease control because they are protective and allow mass *** note,these vaccines elicit undetectable H7N9-specific hemagglutination-inhibition(HI)but high IgG antibodies in ***,the molecular basis and protective mechanism underlying this particular antibody immunity remain ***,immunization with an NDV_(vec)H7N9 induced low anti-H7N9 HI and virus neutralization titers but high levels of hemagglutinin(HA)-binding IgG antibodies in *** residues(S150,G151 and S152)in HA of H7N9 virus were identified as the dominant epitopes recognized by the NDV_(vec)H7N9 immune *** transferred NDV_(vec)H7N9 immune serum conferred complete protection against H7N9 virus infection in *** NDV_(vec)H7N9 immune serum can mediate a potent lysis of HA-expressing and H7N9 virus-infected cells and significantly suppress H7N9 virus *** activities of the serum were significantly impaired after heat-inactivation or treatment with complement inhibitor,suggesting the engagement of the complement ***,mutations in the 150-SGS-152 sites in HA resulted in significant reductions in cell lysis and virus neutralization mediated by the NDV_(vec)H7N9 immune serum,indicating the requirement of antibody-antigen binding for complement ***,antibodies induced by the NDV_(vec)H7N9 can activate antibody-dependent complement-mediated lysis of H7N9 virus-infected cells and complement-mediated neutralization of H7N9 *** findings unveiled a novel role of the complement in protection conferred by the NDV_(vec)H7N9,highlighting a potential benefit of engaging the complement system in H7N9 vaccine design.

关键词： H7N9 subtype avian influenza virus NDV vector vaccine antibody immunity complement protection

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Symmetry dependent activation and reactivity of peroxysulfates on FeS_(2)(001)surface

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Science Bulletin 2024年第2期69卷 154-158页

作者： Cancan Ling Meiqi Li Hao Li xiufan liu Guangming Zhan Hongwei Sun Chuan Liang Yaling Li Bing Zhou Jincai Zhao Lizhi Zhang School of Environmental Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China Key Laboratory of Pesticide&Chemical Biology of Ministry of Education Institute of Environmental&Applied ChemistryCentral China Normal UniversityWuhan 430079China

Heterogeneous peroxysulfates-based advanced oxidation processes(AOPs)have garnered significant attention for purifying organic wastewater since they offer many advantages like low cost,safe storage,facile activation and reactive species participation[1].Nonradical and radical pathways have been proposed to be involved in peroxysulfates activation.

关键词： activation radical reactivity

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Phosphorylation of PB2 at serine 181 restricts viral replication and virulence of the highly pathogenic H5N1 avian influenza virus in mice

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Virologica Sinica 2024年第1期39卷 97-112页

作者： Jiao Hu Zixiong Zeng Xia Chen Manyu Zhang Zenglei Hu Min Gu Xiaoquan Wang Ruyi Gao Shunlin Hu Yu Chen Xiaowen liu Daxin Peng xiufan liu Animal Infectious Disease Laboratory School of Veterinary MedicineYangzhou UniversityYangzhou225009China Joint International Research Laboratory of Agriculture and Agri-Product Safety The Ministry of Education of ChinaYangzhou University225009China Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis Yangzhou UniversityYangzhou225009China Key Laboratory of Prevention and Control of Biological Hazard Factors(Animal Origin)for Agri-food Safety and Quality Ministry of Agriculture of China(26116120)Yangzhou UniversityYangzhou225009China

Influenza A virus(IAV)continues to pose a pandemic threat to public health,resulting a high mortality rate annually and during pandemic *** modification of viral protein plays a substantial role in regulating IAV ***,based on immunoprecipitation(IP)-based mass spectrometry(MS)and purified virus-coupled MS,a total of 89 phosphorylation sites distributed among 10 encoded viral proteins of IAV were identified,including 60 novel phosphorylation ***,for the first time,we provide evidence that PB2 can also be acetylated at site ***,the PB2 S181 phosphorylation site was consistently identified in both IP-based MS and purified virus-based *** S181 and K187 are exposed on the surface of the PB2 protein and are highly conserved in various IAV strains,suggesting their fundamental importance in the IAV life *** analysis results demonstrated that S181E/A and K187Q/R mimic mutations do not significantly alter the PB2 protein *** continuous phosphorylation mimicked by the PB2 S181E mutation substantially decreases viral fitness in mice,PB2 K187Q mimetic acetylation slightly enhances viral virulence in ***,PB2 S181E substantially impairs viral polymerase activity and viral replication,remarkably dampens protein stability and nuclear accumulation of PB2,and significantly weakens IAV-induced inflammatory ***,our study further enriches the database of phosphorylation and acetylation sites of influenza viral proteins,laying a foundation for subsequent mechanistic ***,the unraveled antiviral effect of PB2 S181E mimetic phosphorylation may provide a new target for the subsequent study of antiviral drugs.

关键词： H5N1 influenza virus PB2 Phosphorylation Acetylation Viral fitness Mice

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Enhanced pathogenicity and transmissibility of H9N2 avian influenza virus in mammals by hemagglutinin mutations combined with PB2-627K

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Virologica Sinica 2023年第1期38卷 47-55页

作者： Kaituo liu Yaqian Guo Huafen Zheng Zhuxing Ji Miao Cai Ruyi Gao Pinghu Zhang Xiaowen liu Xiulong Xu Xiaoquan Wang xiufan liu Joint International Research Laboratory of Agriculture and Agri-Product Safety The Ministry of Education of ChinaYangzhou UniversityYangzhou225009China Animal Infectious Disease Laboratory College of Veterinary MedicineYangzhou UniversityYangzhou225009China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis Yangzhou UniversityYangzhou225009China Jiangsu Key Laboratory of Zoonosis Yangzhou225009China Institute of Translational Medicine Key Laboratory of Geriatric Disease Prevention and Control of Jiangsu ProvinceMedical CollegeYangzhou UniversityYangzhou225009China

H9N2 avian influenza viruses(AIVs)circulate globally in poultry and have become the dominant AIV subtype in China in recent ***,we demonstrated that the H9N2 virus(A/chicken/Eastern China/SDKD1/2015)naturally harbors a mammalian-adaptive molecular factor(627K)in the PB2 protein and is weakly pathogenic in ***,we focused on new markers for virulence in mammals.A mouse-adapted H9N2 virus was serially passaged in mice by infecting their *** expected,infected mice showed clinical symptoms and died at passage six.A comparison between the wild-type and mouse-adapted virus sequences identified amino acid substitutions in the hemagglutinin(HA)protein.H9N2 viruses with the T187P t M227L double mutation exhibited an increased affinity to human-type(SAα2,6Gal)receptors and significantly enhanced viral attachment to mouse lung tissues,which contributed to enhancing viral replication and virulence in ***,HA with the T187P t M227L mutation enabled H9N2 viral transmission in guinea pigs via direct *** pathogenicity in mice is a polygenic *** results demonstrated that these HA mutations might be combined with PB2-627K to significantly increase H9N2 virulence in mice,and this enhanced virulence was achieved in other H9N2 AIVs by generating the same combination of *** summary,our study identified novel key elements in the HA protein that are required for H9N2 pathogenicity in mice and provided valuable insights into pandemic preparedness against emerging H_(9)N_(2)strains.

关键词： H9N2 Hemagglutinin(HA) PB2-627K Mammalian adaptation Pathogenicity Transmissibility

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2019—2021年华东地区6株禽源H3亚型流感病毒的遗传进化分析

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中国动物传染病学报 2024年第2期32卷 208-217页

作者：赵婉宸李扬才天宇葛志闯高如一王晓泉顾敏刘秀梵扬州大学兽医学院农业农村部禽用生物制剂创制重点实验室扬州225009 江苏省人兽共患病学重点实验室扬州225009 江苏高校动物重要疫病与人兽共患病防控协同创新中心扬州225009

H3亚型流感病毒在自然界广泛存在,可感染人、猪、马、犬、禽等众多宿主并能发生跨种间传播,危害不容忽视。本研究于2019—2021年在华东地区活禽市场采集表观健康的家禽喉头与泄殖腔棉拭,通过血清学试验鉴定、筛选出6株代表性水禽源H3亚... 详细信息

H3亚型流感病毒在自然界广泛存在,可感染人、猪、马、犬、禽等众多宿主并能发生跨种间传播,危害不容忽视。本研究于2019—2021年在华东地区活禽市场采集表观健康的家禽喉头与泄殖腔棉拭,通过血清学试验鉴定、筛选出6株代表性水禽源H3亚型流感病毒,并经RT-PCR试验确定5株为H3N2亚型、1株为H3N1亚型。进一步对6株分离株进行全基因组测序、同源性比对和遗传进化分析,结果显示:HA蛋白裂解位点处均为PEKQTR/GLF,不存在连续碱性氨基酸,符合低致病性禽流感病毒的分子特征;受体结合位点处均为禽源特征性的226Q和228S,提示其跨种传播至哺乳动物的潜能较低;内部基因片段来源多样化,与H3N8、H4N6、H5N8、H6N1、H7N7、H0N3等众多亚型禽流感病毒的亲缘关系密切;除JS1018/19和JS1094/19的NS基因属于北美谱系以外,其余均属于欧亚谱系的禽源分支,与犬、马、猪、人等哺乳动物源毒株的亲缘关系较远,提示虽然可能存在不同进化谱系间的基因重组但并未发生从禽到哺乳动物宿主的基因交换。该研究结果丰富了H3亚型禽流感病毒的流行病学数据,为进一步了解我国低致病性禽流感病毒的分布特征提供了参考依据。

关键词：禽流感病毒 H3N1 H3N2 基因组分析遗传进化

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西延高铁黄土路基高边坡生态防护技术

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铁路技术创新 2024年第1期 18-24页

作者：庞晓林史丽涛武玉坤牛铭杰刘秀帆西成铁路客运专线陕西有限责任公司陕西西安710000 中铁二十局集团第二工程有限公司陕西西安710000 中国科学院西北生态环境资源研究院沙漠与沙漠化重点实验室甘肃兰州730000

黄土高原特殊的地质条件与土力学特征导致黄土地区滑坡问题严重。黄土地区交通线路,尤其是高铁路基边坡生态防护(修复)已成为水土保持与工程地质等领域的重要研究问题。以西延高铁黄土路基为例,从黄土地区滑坡的致灾机理、黄土路基边坡... 详细信息

黄土高原特殊的地质条件与土力学特征导致黄土地区滑坡问题严重。黄土地区交通线路,尤其是高铁路基边坡生态防护(修复)已成为水土保持与工程地质等领域的重要研究问题。以西延高铁黄土路基为例,从黄土地区滑坡的致灾机理、黄土路基边坡的防护措施及黄土地区生态修复技术3个方面,梳理黄土路基边坡生态防护(修复)研究取得进展、适用范围以及未来发展趋势;从工程措施、生物措施和排水措施3个方面,论述西延高铁采用的具体边坡防护技术,并对黄土路基高边坡生态防护技术集成研究提出展望,为黄土地区生态修复及黄土路基边坡绿色防护研究提供参考。

关键词：西延高铁黄土路基高边坡致灾机理防护措施生态修复

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Emergence of a novel reassortant avian influenza virus(H10N3) in Eastern China with high pathogenicity and respiratory droplet transmissibility to mammals

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Science China(Life Sciences) 2022年第5期65卷 1024-1035页

作者： Kaituo liu Pingyun Ding Yuru Pei Ruyi Gao Wenwen Han Huafen Zheng Zhuxing Ji Miao Cai Jinyuan Gu Xiuli Li Min Gu Jiao Hu Xiaowen liu Shunlin Hu Pinghu Zhang Xiaobo Wang Xiaoquan Wang xiufan liu College of Veterinary Medicine Yangzhou UniversityYangzhou 225009China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis Yangzhou UniversityYangzhou 225009China Jiangsu Key Laboratory of Zoonosis Yangzhou 225009China Institute of Translational Medicine Key Laboratory of Geriatric Disease Prevention and Control of Jiangsu ProvinceMedical CollegeYangzhou UniversityYangzhou 225009China

Decades have passed since the first discovery of H10-subtype avian influenza virus(AIV) in chickens in 1949,and it has been detected in many species including mammals such as minks,pigs,seals and *** of human infections with H10N8viruses identified in China in 2013 have raised widespread *** novel reassortant H10N3 viruses were isolated from chickens in December 2019 in eastern China during routine surveillance for *** internal genes of these viruses were derived from genotype S(G57) H9N2 and were consistent with H5N6,H7N9 and H10N8,which cause fatal infections in *** viral pathogenicity and transmissibility were further studied in different animal *** two H10N3 isolates had low pathogenicity in chickens and were transmitted between chickens via direct *** viruses were highly pathogenic in mice and could be transmitted between guinea pigs via direct contact and respiratory *** importantly,these viruses can bind to both human-type SAα-2,6-Gal receptors and avian-type SAα-2,3-Gal *** shedding in chickens and good adaptability to mammals of these H10N3 isolates would make it easier to transmit to humans and pose a threat to public health.

关键词： H10N3 H9N2 receptor binding pathogenicity respiratory droplet transmissibility

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Endemicity of H9N2 and H5N1 avian influenza viruses in poultry in China poses a serious threat to poultry industry and public health

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Frontiers of Agricultural Science and Engineering 2016年第1期3卷 11-24页

作者： Jiao HU xiufan liu Animal Infectious Disease Laboratory School of Veterinary MedicineJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis Yangzhou University

The H9N2 and H5N1 avian in fluenza viruses(AIVs) have been circulating in poultry in China and become endemic since 1998 and 2004, ***, they are prevalent in poultry throughout China. This endemicity makes them actively involved in the emergence of the novel lineages of other subtypes of in fluenza viruses, such as the well-known viruses of the highly pathogenic avian in fluenza(HPAI) H5N2 and the2013 novel H7N7, H7N9 and H10N8 subtypes, thereby threatening both the poultry industry and public ***, we will review brie fly the prevalence and evolution,pathogenicity, transmission, and disease control of these two subtypes and also discuss the possibility of emergence of potentially virulent and highly transmissible AIVs to humans.

关键词： avian influenza virus H9N2 H5N1 novel viruses public health

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MicroRNA transcriptome analysis of chicken embryo fibroblast cells infected with Newcastle disease virus variants

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Animal Diseases 2023年第3期3卷 166-177页

作者： Weiwei liul Zejun Xu Siyuan Wang Cuiping Song Xusheng Qiu Lei Tan Yingjie Sun Ying Liaol Gujun Wang xiufan liu Chan Ding Shanghai Veterinary Research Institute Chinese Academy of Agricultural SciencesShanghaiChina School of Food and Bioengineering Wuhu Institute of TechnologyWuhuChina School of Veterinary Medicine South China Agricultural UniversityGuangzhouChina School of Veterinary Medicine Anhui Agricultural UniversityHefeiChina School of Veterinary Medicine Yangzhou UniversityYangzhouChina Jjangsu Coinnovation Center for Prevention and Control flmportant Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhouChina

Variations in the pathogenicity of Newcastle disease virus(NDV),the agent causing Newcastle disease,are associated with variants of different virulence.A few studies have characterized the expression of microRNAs(miRNAs)in NDV-infected avian ***,the expression of miRNAs in chicken embryo fibroblasts(CEFs)infected with Herts/33 and LaSota NDV strains(highly virulent and nonvirulent,respectively)was determined using RNA *** involved in NDV infection included 562 previously documented and 184 novel *** target genes involved transcription factors,cell apoptosis,ubiquitin-mediated proteolysis,and protein processing in the endoplasmic *** target genes associated with autophagy were verified by *** studies have documented the miRNA profles of CEFs infected with NDVs *** study adds to our knowledge of the cellular miRNAs involved in NDV infection and the complex molecular mechanisms mediating virus-host *** results of this study will aid the development of strategies against the chicken virus.

关键词： CEF Chicken microRNA Newcastle disease virus RNA-seq

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Modification of the full-length cDNA clone of Newcastle disease virus isolated from an outbreak in the goose

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Frontiers in Biology 2006年第4期1卷 389-393页

作者： liu Yuliang HU Shunli ZHANG Yanmei WU Yantao liu xiufan Röemer-Oberdoerfer Angela Veits Jutta Lange Martina Key Laboratory of Animal Infectious Diseases Ministry of AgricultureYangzhou UniversityYangzhou 225009China Institute of Molecular Biology Friedrich-Loeffler-InstitutesFederal Research Centre for Virus Diseases of AnimalsD-17498 Insel RiemsGermany

A 6.5-kb specific fragment containing the T7 promoter and the transcription vector was excised from the full-length cDNA clone of the Newcastle disease virus(NDV)strain ZJI of goose origin,and thereafter it was self-ligated to form a high quality plasmid for ***-directed mutagenesis was used for inserting three additional G nucleotides(nts)into the region between the T7 promoter and the leader sequence of the NDV ***-PCR was employed to amplify the F/HN gene fragments,and then they were ligated by the shared restriction enzyme ***,the corresponding fragment in the mutant full-length cDNA was substituted with the new *** sequencing results showed that the three additional G nts were successfully inserted and the mutant nts in the full-length cDNA were *** study lays a good foundation for research on the reverse genetics of NDV strain ZJI.

关键词： Newcastle disease virus goose site-directed mutagenesis genomic cDNA clone modification

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