检索结果-南通市图书馆

Determination of dietary copper requirement by the monoamine oxidase activity in kidney of broilers from 1 to 21 days of age

引用

Animal Nutrition 2022年第1期8卷 227-234页

作者： Yun Hu Zhiyong Chen Lin Lu Liyang Zhang Tao Liu Xugang Luo xiudong liao Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou225000China Mineral Nutrition Research Division State Key Laboratory of Animal NutritionInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China Biochemistry Department Case Western Reserve UniversityCleveland44106USA

The current dietary copper(Cu)requirement(8 mg/kg)of broilers is mainly based on growth,hemoglobin concentration,or hematocrit,which might not be the most sensitive indices to evaluate dietary Cu requirements of *** present study was carried out to estimate dietary Cu requirements of broilers fed a conventional corn-soybean meal diet from 1 to 21 d of age using biochemical or molecular biomarkers.A total of 3841-d-old Arbor Acres male broilers were randomly allocated to 1 of 6 treatments with 8 replicates and fed a Cu-unsupplemented corn-soybean meal basal diet containing 5.17 mg Cu/kg by analysis and the basal diet supplemented with 3,6,9,12 or 15 mg Cu/kg as CuSO4,5H2O for 21 *** analysis was performed to estimate the optimal dietary Cu level using the broken-line *** supplemental Cu level affected(P0.05)on the growth performance,Cu contents in heart,kidney,pancreas and spleen,Cu-and zinc-containing superoxide dismutase(CuZnSOD)activity and ceruloplasmin content in serum,CuZnSOD and cytochrome c oxidase(COX)activities and ceruloplasmin,CuZnSOD,MAO A,MAO B,COX 4I1 and COX 1 mRNA and protein expressions in the above tissues of *** dietary supplemental Cu levels increased,Cu contents in serum and liver increased linearly(P<0.05),but kidney MAO activity decreased linearly and quadratically(P<0.05).The estimated dietary Cu requirement based on the fitted broken-line model(P=0.035)of kidney MAO activity was 11.30 mg/*** conclusion,kidney MAO activity is a new and sensitive criterion to evaluate the dietary Cu requirement of broilers,and the dietary Cu requirement was 11.30 mg/kg for broilers fed the conventional corn-soybean meal diet from 1 to 21 d of age,which is higher than the current National Research Council(NRC)Cu requirement(8 mg/kg)of broilers.

关键词： Copper Monoamine oxidase Requirement Broilers

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Metabolic utilization of intravenously injected iron from different iron sources in target tissues of broiler chickens

引用

Animal Nutrition 2022年第2期9卷 74-83页

作者： Lin Lu Xueyu Dong Xuelian Ma Liyang Zhang Sufen Li Xugang Luo xiudong liao Mineral Nutrition Research Division State Key Laboratory of Animal NutritionInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou225000China Department of Animal Science Hebei Normal University of Science and TechnologyQinhuangdao066004China

No information is available regarding the utilization of iron (Fe) from different Fe sources at a targettissue level. To detect differences in Fe metabolic utilization among Fe sources, the effect of intravenouslyinjected Fe on growth performance, hematological indices, tissue Fe concentrations and Fe-containingenzyme activities and gene expressions of Fe-containing enzymes or protein in broilers was *** d 22 post-hatching, a total of 432 male chickens were randomly allotted to 1 of 9 treatments ina completely randomized design. Chickens were injected with either a 0.9% (wt/vol) NaCl solution(control) or a 0.9% NaCl solution supplemented with Fe sulphate or 1 of 3 organic Fe sources. The 3organic Fe sources were Fe chelates with weak (Fe-MetW), moderate (Fe-ProtM) or extremely strong (Fe-ProtES) chelation strength. The 2 Fe dosages were calculated according to the Fe absorbabilities of 10%and 20% every 2 d for a duration of 20 d. Iron injection did not affect (P > 0.05) ADFI, ADG or FCR duringeither 1 to 10 d or 11 to 20 d after injections. Hematocrit and Fe concentrations in the liver and kidney ond 10 after Fe injections, and Fe concentrations in the liver or pancreas and ferritin heavy-chain (FTH1)protein expression level in the liver or spleen on d 20 after Fe injections increased (P≤0.05) as injectedFe dosages increased. When the injected Fe level was high at 20% Fe absorbability, the chickens injectedwith Fe-ProtES had lower (P < 0.001) liver or kidney Fe concentrations and spleen FTH1 protein levelsthan those injected with Fe-MetW or Fe-ProtM on d 20 after injections. And they had lower (P < 0.05)liver cytochrome C oxidase mRNA levels on d 20 after injections than those injected with Fe-MetW or Fesulphate. The results from this study indicate that intravenously injected Fe from Fe-ProtES was the least utilizable and functioned in the sensitive target tissue less effectively than Fe from Fe sulfate, Fe-MetW or Fe-ProtM.

关键词： Intravenous injection Iron-containing protein Gene expression Iron source Broiler

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Determination of optimal dietary selenium levels by full expression of selenoproteins in various tissues of broilers from 22 to 42 d of age

引用

Animal Nutrition 2022年第1期8卷 18-25页

作者： Chuanlong Wang Lisai Wang Liyang Zhang Lin Lu Tao Liu Sufen Li Xugang Luo xiudong liao Mineral Nutrition Research Division State Key Laboratory of Animal NutritionInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou225000China Department of Animal Science Hebei Normal University of Science and TechnologyQinhuangdao066004China Biochemistry Department Case Western Reserve UniversityCleveland44106USA

The current NRC dietary selenium(Se)requirement(0.15 mg/kg)of broilers from 22 to 42 d of age is primarily based on a previous study reported in 1986,which might not be applicable to modern classes of rapidly growing *** present experiment was conducted to determine the optimal dietary Se level for meeting metabolic and functional Se requirements of broilers fed a corn-soybean meal diet from 22 to 42 d of age.A total of 336 Arbor Acres male broilers at 22 d old were randomly assigned to 1 of 6 treatments with 7 replicates and fed a basal corn-soybean meal diet(control,containing 0.014 mg Se/kg)and the basal diet supplemented with 0.10,0.20,0.30,0.40,or 0.50 mg Se/kg from Na_(2)SeO_(3) for 21 *** results showed that the Se concentrations in plasma,liver,kidney,pancreas,breast and thigh muscles,the activity of glutathione peroxidase(GPX)in plasma,liver and kidney,the mRNA expression levels of Gpx4,selenoprotein(Seleno)h and Selenou in liver,Selenop and Selenoh in kidney,and the protein expression levels of GPX4 in the liver and kidney of broilers were affected(P<0.05)by supplemental Se level,and increased quadratically(P<0.05)with the increase of supplemental Se *** estimates of optimal dietary Se levels were 0.10 to 0.49 mg/kg based on the fitted broken-line or asymptotic models(P<0.0001)of the above Se concentration indices,and 0.08 to 0.37 mg/kg based on the fitted brokenline,quadratic or asymptotic models(P<0.007)of the above selenoprotein expression *** results indicate that the optimal dietary Se levels would be 0.49 mg/kg to support the maximum Se concentrations and 0.37 mg/kg to support the full expression of selenoproteins in plasma and various tissues of broilers fed a corn-soybean meal diet from 22 to 42 d of age.

关键词： Selenium Selenoprotein Gene expression Requirement Broiler

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Regulation of bone phosphorus retention and bone development possibly by related hormones and local bone-derived regulators in broiler chicks

引用

Journal of Animal Science and Biotechnology 2022年第1期13卷 224-239页

作者： Sumei Cao Tingting Li Yuxin Shao Liyang Zhang Lin Lu Rijun Zhang Shuisheng Hou Xugang Luo xiudong liao Mineral Nutrition Research Division State Key Laboratory of Animal NutritionInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193People’s Republic of China Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225000People’s Republic of China Laboratory of Feed Biotechnology State Key Laboratory of Animal NutritionCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193People’s Republic of China

Background:Phosphorus is essential for bone mineralization in broilers,however,the underlying mechanisms remain *** aimed to investigate whether bone phosphorus retention and bone development might be regulated by related hormones and local bone-derived regulators in ***:Broilers were fed diets containing different levels of non-phytate phosphorus(NPP)0.15%,0.25%,0.35%,0.45% and 0.55% or 0.15%,0.22%,0.29%,0.36% and 0.43% from 1 to 21 or 22 to 42 days of *** and tibia samples were collected for determinations of bone phosphorus retention and bone development parameters,related hormones and local bone-derived regulators of broiler chickens on d 14,28 and 42,***:Tibia ash phosphorus,total phosphorus accumulation in tibia ash(TP_(TA)),bone mineral concentration(BMC),bone mineral density(BMD),bone breaking strength(BBS),and ash on d 14,28 or 42,serum 1,25-dihydroxyvitamin D_(3)(1,25(OH)_(2)D_(3))on d 28 and 42,mRNA expressions of tibia fibroblast growth factor 23(FGF23)and dentin matrix protein 1(DMP1)on d 14 and 28 increased linearly or quadratically(P<0.05),while serum parathyroid hormone(PTH)on d 28,tibia alkaline phosphatase(ALP)on d 14,28 and 42,bone gal protein(BGP)on d 14,and mRNA expression of tibia phosphate-regulating gene with homologies to endopeptidases on the X chromosome(PHEX)on d 14 and 28 decreased linearly or quadratically(P<0.04)as dietary NPP level ***,BMC,BMD,and ash on d 28 and 42,BBS on d 28,and ash phosphorus on d 42 were positively correlated(r=0.389 to 0.486,P<0.03)with serum 1,25(OH)_(2)D_(3).All of the above parameters were positively correlated(r=0.380 to 0.689,P<0.05)with tibia DMP1 mRNA expression on d 14,28 and 42,but negatively correlated(r=−0.609 to−0.538,P<0.02)with serum PTH on d 28,tibia ALP on d 14,28 and 42,and BGP on d ***_(TA),BMC and ash on d 14 and BMD on d 28 were negatively correlated(r=−0.397 to−0.362,P<0.03)with tibia PHEX mRNA expression,and BMD on d 28 was positively

关键词： Bone Broiler chicken Hormone Local bone-derived regulator Phosphorus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Zinc alleviates the heat stress of primary cultured hepatocytes of broiler embryos via enhancing the antioxidant ability and attenuating the heat shock responses

引用

Animal Nutrition 2021年第3期7卷 621-630页

作者： Tingting Li Wengang He xiudong liao Xi Lin Liyang Zhang Lin Lu Yanli Guo Zongping Liu Xugang Luo Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009China Mineral Nutrition Research Division Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China College of Animal Science and Technology Gansu Agricultural UniversityLanzhou 730070China Department of Animal Science North Carolina State UniversityRaleighNC 27695USA College of Veterinary Medicine Yangzhou UniversityYangzhou 225009China

Zinc(Zn)has been shown to attenuate the adverse effects of heat stress on broilers,but the mechanisms involving this process remain *** aimed to investigate possible protective mechanisms of Zn on primary cultured hepatocytes of broiler embryos subjected to heat *** experiments were *** Exp.1,hepatocytes were treated with 0,50,100,200,or 400μmol/L added Zn as inorganic Zn sulfate(iZn)for 12,24 or 48 *** Exp.2,cells were exposed to 40℃(a normal temperature[NT])and 44℃(a high temperature[HT])for 1,2,4,6,or 8 *** Exp.3,cells were preincubated with 0 or 50μmol/L Zn as iZn or organic Zn lysine chelate(oZn)for 8 h under NT,and then incubated with the same Zn treatments under NT or HT for 4 or 6 *** biomarkers of antioxidative status and heat stress in cells were *** results in Exp.1 indicated that 50μmol/L Zn and 12 h incubation were the optimal conditions for increasing antioxidant ability of *** Exp.2,the 4 or 6 h incubation under HT was effective in inducing heat shock responses of *** Exp.3,HT elevated(P<0.01)malondialdehyde content and expressions of heat shock protein 70(HSP70)mRNA and protein,as well as HSP90 ***,Zn supplementation increased(P<0.05)copper zinc superoxide dismutase(CuZnSOD)activity and metallothionein mRNA expression,and effectively decreased(P<0.05)the ex-pressions of HSP70 mRNA and protein,as well as HSP90 ***,oZn was more effective(P<0.05)than iZn in enhancing CuZnSOD activity of hepatocytes under *** was concluded that Zn(especially oZn)could alleviate heat stress of broiler hepatocytes via enhancing their antioxidant ability and attenuating heat shock responses.

关键词： Broiler hepatocyte Zinc Heat stress Antioxidant ability Heat shock response

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dietary calcium requirements of broilers fed a conventional corn-soybean meal diet from 1 to 21 days of age

引用

Journal of Animal Science and Biotechnology 2022年第4期13卷 1163-1174页

作者： Shiping Bai Yunfeng Yang Xuelian Ma xiudong liao Runlian Wang Liyang Zhang Sufen Li Xugang Luo Lin Lu Mineral Nutrition Research Division State Key Laboratory of Animal NutritionInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225000China Institute of Animal Nutrition Sichuan Agricultural UniversityChengdu 611130SichuanChina Department of Animal Science Guangdong Ocean UniversityZhanjiang 524088China Department of Animal Science Hebei Normal University of Science and TechnologyQinhuangdao 066004China.

Background:The current calcium(Ca)recommendation for broilers is primarily based on studies conducted more than 30 years ago with birds of markedly different productive potentials from those which exist *** the response indicators in these studies are mainly growth performance and bone ash ***,the present study was carried out to investigate the effect of dietary Ca level on growth performance,serum parameters,bone characteristics and Ca metabolism-related gene expressions,so as to estimate dietary Ca requirements of broilers fed a conventional corn-soybean meal diet from 1 to 21 days of ***:A total of 4201-day-old Arbor Acres male broilers were randomly assigned to 1 of 7 treatments with 6 replicates(10 birds per cage)and fed the corn-soybean meal diets containing 0.60%,0.70%,0.80%,0.90%,1.00%,1.10%or 1.20%Ca for 21 *** diet contained the constant non-phytate phosphorus content of about 0.39%.Results:The average daily gain decreased linearly(P<0.001)as dietary Ca level *** serum and tibia alkaline phosphatase(ALP)activities,tibia bone mineral density(BMD),middle toe BMD,tibia ash percentage,tibia breaking strength,and tibia ALP protein expression level were affected(P<0.05)by dietary Ca level,and showed significant quadratic responses(P<0.02)to dietary Ca *** estimates of dietary Ca requirements were 0.80 to 1.00%based on the best fitted broken-line or quadratic models(P<0.03)of the above serum and bone parameters,***:The results from the present study indicate that the Ca requirements would be about 0.60%to obtain the best growth rate,and 1.00%to meet all of the Ca metabolisms and bone development of broilers fed a conventional corn-soybean meal diet from 1 to 21 days of age.

关键词： Bone characteristic Broiler Calcium Gene expression Requirement

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Determination of optimal dietary selenium levels by full expression of selenoproteins in various tissues of broilers from 1 to 21 d of age

引用

Animal Nutrition 2021年第4期7卷 1133-1144页

作者： xiudong liao Guoqing Liu Guangming Sun Xiaoming Sun Tao Liu Lin Lu Liyang Zhang Minhong Zhang Yanli Guo Xugang Luo Mineral Nutrition Research Division State Key Laboratory of Animal NutritionInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijingChina Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhouChina College of Animal Science and Technology Gansu Agricultural UniversityLanzhouChina Biochemistry Department Case Western Reserve UniversityClevelandUSA State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement LhasaChina Institute of Animal Science and Veterinary Tibet Academy of Agricultural and Animal Husbandry SciencesLhasaChina

The current NRC dietary selenium(Se)requirement(0.15 mg/kg)of broilers is primarily based on growth performance data reported in *** study aimed to determine optimal dietary Se levels of broilers fed a practical corn-soybean meal diet for the full expression of selenoproteins in various tissues.A total of 384 one-d-old male broilers(n=8 replicates/diet)were fed a basal corn-soybean meal diet or the basal diet supplemented with 0.1,0.2,0.3,0.4 or 0.5 mg Se/kg in the form of Na_(2)SeO_(3) for 21 *** analysis was conducted to evaluate the optimal dietary Se levels using broken-line,quadratic or asymptotic *** activity of glutathione peroxidase(GPX)in the plasma,liver,kidney and pancreas,iodothyronine deiodinase(DIO)in the plasma,liver and pancreas,and thioredoxin reductase(Txnrd)in the liver and pancreas,the mRNA levels of Gpx1,Gpx4,Dio1,selenoprotein(Seleno)h,Selenop and Selenou in the liver,Gpx4,Dio1,Txnrd1,Txnrd2,Selenoh,Selenop and Selenou in the kidney,and Gpx1,Gpx4,Selenoh and Selenou in the pancreas,and the protein levels of GPX4 in the liver and kidney of broilers were influenced(P<0.05)by added Se levels,and increased quadratically(P<0.05)with the increase of added Se *** estimates of optimal dietary Se levels were 0.07 to 0.36 mg/kg based on the fitted broken-line,quadratic or asymptotic models(P<0.001)of the aforementioned selenoprotein expression in the plasma,liver and kidney,and 0.09 to 0.46 mg/kg based on the fitted broken-line models(P<0.001)of the aforementioned selenoprotein expression in the *** results indicate that the optimal dietary Se levels would be 0.36 mg/kg to support the full expression of selenoproteins in the plasma,liver and kidney,and 0.46 mg/kg to support the full expression of selenoproteins in the pancreas of broilers fed a practical corn-soybean meal diet from 1 to 21 d of age.

关键词： Selenium Selenoprotein Gene expression Requirement Broiler

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

我国畜禽饲料资源中矿物元素含量分布的调查

引用

中国农业科学 2019年第11期52卷 1970-1972页

作者：廖秀冬张丽阳吕林罗绪刚中国农业科学院北京畜牧兽医研究所矿物元素营养研究室

饲料是畜牧业赖以发展的物质基础,饲料成本约占整个畜禽养殖成本的60%—70%。三十多年来,我国饲料工业取得了巨大成就,走过了许多发达国家五十年甚至上百年才走完的历程,实现了世界第一大饲料生产国的飞跃,2018年我国饲料总产量已达2.2... 详细信息

饲料是畜牧业赖以发展的物质基础,饲料成本约占整个畜禽养殖成本的60%—70%。三十多年来,我国饲料工业取得了巨大成就,走过了许多发达国家五十年甚至上百年才走完的历程,实现了世界第一大饲料生产国的飞跃,2018年我国饲料总产量已达2.28亿吨[1]。我国饲料工业的快速发展,有力地推动了我国畜牧经济的发展,改善了人们的物质生活水平。近年来,我国畜禽养殖业规模化、集约化发展迅速,饲料企业的规模和产品结构也发生了较大变化。一些小型饲料企业逐渐被淘汰,规模生产企业占绝对主导地位,配合饲料的比例越来越高,

关键词：畜禽饲料矿物元素饲料资源含量分布

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

低磷浓度下原代培养肉鸡鸡胚十二指肠上皮细胞对磷吸收及相关转运载体表达的研究

引用

动物营养学报 2019年第9期31卷 4186-4193页

作者：吕娜吕林廖秀冬张丽阳罗绪刚中国农业科学院北京畜牧兽医研究所矿物元素营养研究室

本试验旨在研究低磷浓度下原代培养肉鸡鸡胚十二指肠上皮细胞对磷吸收及相关磷转运载体的表达。试验采用单因子完全随机设计,设0(对照组)、0.5、1.0、2.0和4.0 mmol/L 5个不同磷浓度组,每组6个重复,孵育时间为87 min。结果表明:不同磷... 详细信息

本试验旨在研究低磷浓度下原代培养肉鸡鸡胚十二指肠上皮细胞对磷吸收及相关磷转运载体的表达。试验采用单因子完全随机设计,设0(对照组)、0.5、1.0、2.0和4.0 mmol/L 5个不同磷浓度组,每组6个重复,孵育时间为87 min。结果表明:不同磷浓度对十二指肠上皮细胞中磷吸收率有显著影响(P0.05)。不同磷浓度对十二指肠上皮细胞中NaPi-Ⅱb、PiT-1和PiT-2蛋白表达无显著影响(P>0.05)。由此可见,随着孵育液磷浓度的增加,细胞磷吸收率增加;当孵育液中磷浓度为4.0 mmol/L时,原代培养肉鸡鸡胚十二指肠上皮细胞可通过下调NaPi-Ⅱb和PiT-1的mRNA表达来调节磷的吸收。

关键词：十二指肠上皮细胞鸡胚磷吸收

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

铁水平和孵育时间对原代培养肉鸡鸡胚肝细胞中铁含量及含铁酶活性、基因表达和蛋白表达的影响

引用

动物营养学报 2020年第8期32卷 3830-3842页

作者：马雪莲廖秀冬张丽阳吕林罗绪刚中国农业科学院北京畜牧兽医研究所矿物元素营养研究室北京100193 扬州大学动物科技学院家禽矿物元素营养研究室扬州225000

本试验旨在研究铁水平和孵育时间对原代培养肉鸡鸡胚肝细胞中铁含量及含铁酶活性、基因表达和蛋白表达的影响。采用5×3两因子完全随机设计,铁水平分别为0、0.25、0.50、0.75、1.00 mmol/L,孵育时间分别为24、48和72 h,共形成15个组,每... 详细信息

本试验旨在研究铁水平和孵育时间对原代培养肉鸡鸡胚肝细胞中铁含量及含铁酶活性、基因表达和蛋白表达的影响。采用5×3两因子完全随机设计,铁水平分别为0、0.25、0.50、0.75、1.00 mmol/L,孵育时间分别为24、48和72 h,共形成15个组,每组6个重复。结果表明:1)铁水平、孵育时间及两者的互作对肝细胞铁含量有显著影响(P<0.05)。24、48和72 h时,0.25、0.50、0.75、1.00 mmol/L铁水平组肝细胞中铁含量均显著高于0 mmol/L铁水平组(P<0.05)。2)铁水平和孵育时间对肝细胞中胞琥珀酸脱氢酶(SDH)和细胞色素c氧化酶(COX)活性有显著影响(P<0.05)。与0 mmol/L铁水平组相比,0.25、0.50、0.75和1.00 mmol/L铁水平组肝细胞中SDH活性显著降低(P<0.05);24 h肝细胞中SDH活性显著高于48 h(P<0.05),而72 h又显著高于24和48 h(P<0.05)。与0 mmol/L铁水平组相比,0.75和1.00 mmol/L铁水平组肝细胞中COX活性显著提高(P<0.05);24和48 h肝细胞中COX活性显著高于72 h(P<0.05)。3)铁水平、孵育时间及两者的互作对肝细胞中SDH、过氧化氢酶(CAT)、COX7A2L和铁蛋白重链1(FTH1)的mRNA表达水平有显著影响(P<0.05),铁水平及铁水平与孵育时间的互作对肝细胞中COX1的mRNA表达水平有显著影响(P<0.05)。4)铁水平和孵育时间对肝细胞中COX1的蛋白表达水平有显著影响(P<0.05),铁水平及铁水平与孵育时间的互作对肝细胞中FTH1的蛋白表达水平表达有显著影响(P<0.05)。24和72 h肝细胞中COX1的蛋白表达水平显著高于48 h(P<0.05),1.00 mmol/L铁水平组肝细胞中COX1的蛋白表达水平显著高于其他铁水平组(P<0.05)。由此可见,铁水平和孵育时间可影响原代培养肉鸡鸡胚肝细胞中铁含量,SDH、CAT和COX的活性、基因表达,以及FTH1的基因表达。综合考虑以上指标,适宜铁的水平应为0.50 mmol/L,孵育时间为24 h。

关键词：原代培养肝细胞肉鸡鸡胚铁含铁酶基因表达

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：