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Co-transplantation of Schwann cells and bone marrow stromal cells versus single cell transplantation on repairing hemisected spinal cord injury of rats

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Neural Regeneration Research 2010年第11期5卷 805-813页

作者： Jifei Zhang Geng Wu Fusheng Zhao xiudong jin Department of Histology and Embryology Mudanjiang Medical College Mudanjiang 157011 Heilongjiang Province China Clinical Skills Training Center Mudanjiang Medical College Mudanjiang 157011 Heilongjiang Province China Deoartment of Phvsiology. Mudanjiang Medical Collecle. Mudaniiancl 157011 Heilongjiancl Province China

BACKGROUND： Bone marrow stromal cells （BMSCs） or Schwann cells （SCs） transplantation alone can treat spinal cord injury. However, the transplantation either cell-type alone has disadvantages. The co-transplantation of both cells may benefit structural reconstruction and functional recovery of spinal nerves. OBJECTIVE： To verify spinal cord repair and related mechanisms after co-transplantation of BMSCs and SCs in a rat model of hemisected spinal cord injury. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Department of Histology and Embryology, Mudanjiang Medical College from January 2008 to May 2009. MATERIALS： Rabbit anti-S-100, glial fibrillary acidic protein, neuron specific enolase and neurofilament-200 monoclonal antibodies were purchased from Sigma, USA. METHODS： A total of 100 Wistar rats were used in a model of hemisected spinal cord injury. The rats were randomly assigned to vehicle control, SCs transplantation, BMSCs transplantation, and co-transplantation groups; 25 rats per group. At 1 week after modeling, SCs or BMSCs cultured in vitro were labeled and injected separately into the hemisected spinal segment of SCs and BMSCs transplantation groups through three injection points [5 μL （1 x 107 cells/mL）] cell suspension in each point）. In addition, a 15 μL 1 × 10^7 cells/mL SCs suspension and a 15 μL 1 × 10^7 cells/mL BMSC suspension were injected into co-transplantation group by the above method. MAIN OUTCOME MEASURES： The Basso-Beattie-Bresnahan （BBB） locomotor rating scale and somatosensory evoked potential （SEP） tests were used to assess the functional recovery of rat hind limbs following operation. Structural repair of injured nerve tissue was observed by light microscopy, electron microscopy, immunohistochemistry, and magnetic resonance imaging （MRI）. In vivo differentiation, survival and migration of BMSCs were evaluated by immunofluorescence. RESULTS： BBB scores were significantly greater in all three tra

关键词： bone marrow stromal cells Schwann cells co-transplantation spinal cord injury neural regeneration

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Influence of hypoxia-inducible factor 1-alpha on neuronal apoptosis in a rat model of hypoxia-or hypoxia-ischemia-induced brain injury

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Neural Regeneration Research 2009年第12期4卷 1019-1023页

作者： Lihua Li Yi Qu Li Zhang Xihong Li jinhui Li Meng Mao xiudong jin Dezhi Mu Department of Pediatrics West China Second Hospital Sichuan University Chengdu 610041 Sichuan Province China Departrnent of Pediatrics Bayi Children's Hospital Military General Hospital of Beijing PLA Beijing 100700 China Mudanjiang Medical College Mudanjiang 157011 Heilongjiang Province China

BACKGROUND： In addition to neuroprotective genes, the targeted genes of hypoxia-inducible factor 1α （HIF-1α） include pro-apoptotic genes. However, the influence of HIF-1α on neuronal apoptosis in hypoxia-ischemia remains poorly understood. OBJECTIVE： To investigate the relationship between HIF-1α expression and neuronal apoptosis in hypoxia or hypoxia-ischemia brain injury and to determine the role of HIF-1α in regulating neuronal apoptosis. DESIGN, TIME AND SETTING： A randomized, controlled animal experiment was performed at the Laboratory of Children Neurology of Sichuan University between May 2006 and May 2007. MATERIALS： In situ cell death detected kit was provided by Roche, USA; rabbit anti-mouse HIF-1α polyclonal antibody was purchased from Santa Cruz Biotechnologies, USA; rabbit anti-mouse cleaved caspase-3 polyclonal antibody was purchased from Chemicon, USA. METHODS： A total of 36 Sprague Dawley rats aged 10 days were randomly assigned to 3 groups： sham-surgery, hypoxia, and hypoxia-ischemia, with 12 rats per group. The rats were treated at 3 time points： 4, 8, and 24 hours, with 4 rats per time point. In the hypoxia-ischemia group, the right common carotid artery was exposed and permanently ligated through a midline cervical incision. A 2.5-hour exposure to hypoxia （8% O2/92% N2） was used to induce hypoxia-ischemia injury. In the hypoxia group, rats were exposed to hypoxia without ligation of the common carotid artery. In the sham-surgery group, the common carotid artery was exposed without ligation or hypoxia. MAIN OUTCOME MEASURES： Histopathological changes, HIF-1α and activated caspase-3 protein expression, integrated optical density of positive cells, and apoptosis-positive cells. RESULTS： Hematoxylin and eosin staining showed that neuronal degeneration and edema was most prominent at 24 hours after hypoxia-ischemia. HIF-1α protein expression was significantly upregulated at 4 hours, peaked at 8 hours, and decreased at 24 hours after hypoxia or hypoxia

关键词： HIF-1α apoptosis hypoxia ischemia brain damage

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Influence of Panax quinquefolium saponins on increased intracellular Ca^(2+) in PC12 cells

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Neural Regeneration Research 2009年第3期4卷 225-229页

作者： Lixin Guan xiudong jin Yanhui Chu Yufei Zhang Yan Wu Xin Yi Fengguo Zhai Men clquan Li Department of Pharmacology Mudanjiang Medical University Mudanjiang 157011 Heilongjiang Province China Department of Physiology -Mudanjiang Medical University Mudanjiang - 157011 Heilongjian'g Province China Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy Mudanjiang Medical University Mudanjiang 157011 Heilongjiang ProvinceChina

BACKGROUND： Previous studies have demonstrated that intracellular Ca^2＋（[Ca^2＋]） overload, excitotoxicity, free radical injury, and nitric oxide toxicity are involved in mechanisms of neuronal death in the ischemic brain. OBJECTIVE： To investigate the influence of Panax quinquefo/ium saponins （PQS） on multiple factors-induced Ca^2＋ overload in the rat pheochromocytoma （PC12） cell line. DESIGN, TIME AND SETTING： Intergroup comparison, in vitro study. The experiment was performed at the Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University between November 2007 and April 2008. MATERIALS- In vitro cultured PC12 cells in the logarithmic phase were assigned into blank control, model, and drug treatment groups （10 μmol/L nimodipine; 40 μg/L, 100 μg/L, and 250 μg/L PQS）. Nimodipine was purchased from Jiangsu Yangtze River Pharmacy Group Co., China; PQS （purity 〉 95%, HLPC grade） was provided by School of Basic Medical Sciences, Jilin University. Caffeine, Na2S2O4, L-glutamic acid （Glu）, Fura-2/AM, and calcium ionophore A23187 were purchased from Sigma, USA. METHODS： PC12 cells in the model and drug treatment groups were separately incubated in glucose-free Hank＇s buffered saline solution ＋ Na2S2O4 （2 mmol/L） for 6 hours, Glu （200 μmot/L） plus A23187 （0.05 μmol/L） for 6 hours, KCI （50 mmol/L） for 1 hour, and caffeine （5 mmol/L） for 3 hours to establish models of intracellular Ca^2＋ overload induced by oxygen and glucose deprivation, Glu, A23187, high K＋, or caffeine. In addition, control cells were incubated in high-glucose DMEM culture medium. MAIN OUTCOME MEASURES： [Ca^2＋]i changes in PC12 cells exposed to oxygen-glucose deprivation, Glu, A23187, high K^＋, or caffeine were detected using spectrofluorometer. RESULTS： PQS blocked the [Ca^2＋]i increase induced by oxygen-glucose deprivation, Glu, A23187, high K＋, or caffeine. In particular, high-dose PQS was most effective （P 〈 0.01）. PQS significantly inhibited Glu- or caffeine-induced [Ca^2

关键词： Panax quinquefolium saponins intracellular Ca^2+ PC12 cells oxygen-glucose deprivation Fura-2/AM

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SBPS对CTX增效减毒作用及其免疫学机制的研究

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中国普通外科杂志 2010年第3期19卷 279-281页

作者：宋高臣王桂云董琦金秀东牡丹江医学院生物化学教研室黑龙江牡丹江157011

目的探讨半枝莲多糖(SBPS)对化疗药物环磷酰胺(CTX)的增效减毒作用及其机制。方法采用移植性H22肝癌小鼠,随机分为阴性对照组,CTX处理组,CTX+SBPS联合用药组。连续给药10 d后,测定其抑瘤率、白细胞数、吞噬指数和IL-2活性。结果SBPS能提... 详细信息

目的探讨半枝莲多糖(SBPS)对化疗药物环磷酰胺(CTX)的增效减毒作用及其机制。方法采用移植性H22肝癌小鼠,随机分为阴性对照组,CTX处理组,CTX+SBPS联合用药组。连续给药10 d后,测定其抑瘤率、白细胞数、吞噬指数和IL-2活性。结果SBPS能提高CTX的抑瘤率,减轻CTX的毒副作用,提高单核吞噬细胞功能和IL-2及TNF-α的活性。结论SBPS对化疗药物CTX具有明显的增效减毒作用,其机制可能是通过增强机体的免疫功能而实现的。

关键词：肝肿瘤/药物疗法 SBPS 环磷酰胺增效减毒免疫功能 IL-2 TNF-α

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Highly efficient separation of Sc^(3+) and Y^(3+) in acid solution by a graphene oxide membrane with interlayer sieving

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Journal of Rare Earths 2024年第11期42卷 2166-2171,I0006页

作者： Huixiong Jiang jin-Hang Liu xiudong Chen Xiaohua Cao Xiushen Ye Guosheng Shi School of Chemistry and Chemical Engineering Jiangxi Province Engineering Research Center of Ecological Chemical IndustryJiujiang UniversityJiujiang332005China Shanghai Applied Radiation Institute State Key Lab.Advanced Special SteelShanghai UniversityShanghai200444China Key Laboratory of Comprehensive and Highly Efficient Utilization of Salt Lake Resources Qinghai Institute of Salt LakesChinese Academy of SciencesXining810008China

Sc and Y are key rare earth elements and are widely used in lamp phosphors,lasers and high-performance ***,highly efficient extraction and separation of Sc^(3+) and Y^(3+) is laborious,harmful,slow,and costly,strongly necessitating more efficient extraction and separation ***,we produced hydrated Sc^(3+)-and hydrated Y^(3+)-controlled graphene oxide(GO) membranes and find that both hydrated cations were completely self-rejected by the *** combining this selfrejection effect of the larger hydrated Y^(3+)-controlled GO membrane and the rapid passage of the membrane through the smaller hydrated Sc^(3+),we proposed a strategy to separate Sc^(3+) and Y^(3+) by using a hydrated Y^(3+)-controlled GO *** experimental results show that the permeation rate of Sc^(3+) exceeds that of Y^(3+) when the separation factor reaches 4.02,which can be attributed to the interlayer sieving effects of the GO *** finding illustrates the use of a forward osmosis process with a GO membrane for the efficient separation of Sc^(3+) and Y^(3+) by interlayer sieving,which provides a new effective and eco-friendly method for the separation of rare earth elements.

关键词： Rare earths Graphene oxide membrane Interlayer spacing Membrane separation Forward osmosis

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复方五味子素B对人结肠癌细胞多药耐药性逆转作用及其机制研究

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中国药师 2010年第7期13卷 915-917页

作者：金秀东徐秋玲念红张绪东刘桂莲孙卫牡丹江医学院生理教研室黑龙江牡丹江157011

目的:研究复方五味子素B(γSC)对耐奥沙利铂人结肠癌细胞(THC-8307/OXA)多药耐药性的逆转作用及作用机制。方法:采用噻唑蓝(MTT)比色法检测奥沙利铂(OXA)和γSC的细胞毒性;碱性磷酸酶免疫组织化学法和Western blot检测γSC对THC-8307/OX... 详细信息

目的:研究复方五味子素B(γSC)对耐奥沙利铂人结肠癌细胞(THC-8307/OXA)多药耐药性的逆转作用及作用机制。方法:采用噻唑蓝(MTT)比色法检测奥沙利铂(OXA)和γSC的细胞毒性;碱性磷酸酶免疫组织化学法和Western blot检测γSC对THC-8307/OXA细胞GST-п蛋白水平的影响。结果:γSC(6.0,12.5,25μg·ml^(-1))对人结肠癌细胞(THC-8307)和THC-8307/OXA无显著毒性作用(P>0.05),OXA对THC-8307的IC_(50)为0.06μg·ml^(-1),而对THC-8307/OXA的IC_(50)为2.32μg·ml^(-1),THC-8307/OXA较THC-8307耐药性提高39倍,γSC(6.0,12.5,25μg·ml^(-1))能使OXA对THC-8307/OXA细胞的IC_(50)从2.32μg·ml^(-1)依次下降至0.370,0.128,0.057μg·ml^(-1),逆转倍数分别为6.2,18.1,40.7倍。碱性磷酸酶免疫组织化学法和Western blot检测γSC(12.5μg·ml^(-1))处理48 h后,THC-8307/OXA细胞GST-п蛋白表达明显降低(P<0.01)。结论:γSC具有逆转耐奥沙利铂人结肠癌细胞的MDR作用,其作用机制与下调GST-п表达有关。

关键词：复方五味子素B 奥沙利铂耐奥沙利铂人结肠癌细胞多药耐药谷胱甘肽S-转移酶

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生理教学中应用情境教学模式的研究

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中国现代教育装备 2010年第23期 76-78页

作者：郑学芝金秀东张绪东刘桂莲孙卫念红李丽徐秋玲牡丹江医学院黑龙江牡丹江157011

为提高生理学授课效率和教学质量,激发同学的学习兴趣,探讨了在生理学教学中创设并应用情境教学模式的科学性、原则、方法及应用效果。

关键词：生理学情境教学医学

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术后滴眼液应用方法研究

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中国继续医学教育 2019年第11期11卷 132-135页

作者：刘爱洁林琳李丽丽李艳洁杨金云金秀东郭永丽内蒙古赤峰市医院眼科内蒙古赤峰024000

使用滴眼液是眼科最基础的操作之一,在临床和家庭中均广泛应用。临床实践中发现,应用传统滴眼液的方法,患眼不容易有效地吸收,易造成药液浪费和污染。为了在患者术后眼的各种被动条件限制下,能够安全、正确地使用滴眼液,并且取得最佳疗... 详细信息

使用滴眼液是眼科最基础的操作之一,在临床和家庭中均广泛应用。临床实践中发现,应用传统滴眼液的方法,患眼不容易有效地吸收,易造成药液浪费和污染。为了在患者术后眼的各种被动条件限制下,能够安全、正确地使用滴眼液,并且取得最佳疗效。临床协助患者提供最佳位置,避开术中切口或探头入口用药,不继续损伤眼睑和结膜。最新推出滴眼液滴用首优点位、次优点位的内容及应用,使结膜囊内的用药盲区减小或消失。对于使用滴眼液的数量、是否按压泪囊也根据需要给予改变。发现为患者的术后眼使用滴眼液,因疾病、术式、切口或探头入口而有异同,只有相应地改变方法,才能够得到满意的疗效。

关键词：术后滴眼液避开切口用药盲区位置配合首优点位

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急性铅染毒老年大鼠脂质过氧化作用

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国际老年医学杂志 2018年第4期39卷 180-182,186页

作者：黄志宝石永萍张慧靳革崔新宇荣胜忠尹永奎谷红梅崔新刚陈莹莹金秀东李淼晶黑龙江省牡丹江医学院中国牡丹江157000 黑龙江省牡丹江市第二人民医院神经内科中国牡丹江157000 黑龙江省牡丹江市卫生监督所中国牡丹江157000

目的观察急性铅染毒老年大鼠脑组织的脂质过氧化作用。方法40只雄性Wistar老年大鼠随机分为4组，每组10只。低、中、高剂量组大鼠分别予以25、50、100mg／kg醋酸铅染毒，隔日染毒1次，连续3次，对照组予以同等体积的生理盐水。染毒后对... 详细信息

目的观察急性铅染毒老年大鼠脑组织的脂质过氧化作用。方法40只雄性Wistar老年大鼠随机分为4组，每组10只。低、中、高剂量组大鼠分别予以25、50、100mg／kg醋酸铅染毒，隔日染毒1次，连续3次，对照组予以同等体积的生理盐水。染毒后对大鼠行为、体重及血浆、脑匀浆丙二醛（MDA）和总超氧化物歧化酶（T—SOD）进行测定。结果与对照组相比，低剂量组、中剂量组、高剂量组体重明显下降（P〈0．05），血铅水平明显升高（P〈0．05），铅染毒老年大鼠易激惹、多动。与对照组比较，各铅染毒组血浆MDA和T—SOD及脑组织匀浆MDA有随着铅染毒剂量增大而增加的趋势，其中高剂量组和中剂量组血浆MDA、T—SOD和脑匀浆MDA明显增高（P〈0．05），低剂量组与对照组比较差异无统计学意义，各组脑匀浆T—SOD活性比较无统计学意义。结论急性铅染毒能够影响老年大鼠的行为，呈现激惹、多动等症状；急性铅染毒能提高老年大鼠脂质过氧化水平，并可代偿性提高机体T—SOD活性，确定铅染毒对老年大鼠神经系统具有毒性作用。

关键词：铅中毒脂质过氧化血铅丙二醛超氧化物歧化酶

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一种无扫描三维成像激光雷达的实验研究

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中国激光 2009年第6期36卷 1383-1387页

作者：靳辰飞赵远张勇何姜孙秀冬哈尔滨工业大学物理系黑龙江哈尔滨150001

研究了一种无扫描三维成像激光雷达。采用脉冲固体激光器作为发射光源,而带有像增强器的CCD(ICCD)作为成像探测器。激光器发射光脉冲的同时用随时间单调变化的高压调制ICCD中的像增强器,使其产生随时间单调变化的增益调制,这样不同距离... 详细信息

研究了一种无扫描三维成像激光雷达。采用脉冲固体激光器作为发射光源,而带有像增强器的CCD(ICCD)作为成像探测器。激光器发射光脉冲的同时用随时间单调变化的高压调制ICCD中的像增强器,使其产生随时间单调变化的增益调制,这样不同距离的目标回波被放大的程度不同。通过对被放大信号能量的比较分析,就可以解算各个脉冲的飞行时间,从而得到各个脉冲对应的距离。结果验证了这种成像系统可有效地消除回波强度不均匀性的影响。通过室内成像实验验证系统成像,在35～41 m的距离上成功地获取了目标的距离像,测距精度可达l m以下。

关键词：激光技术激光雷达增益调制距离像

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