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Plant Communications 2024年第2期5卷 135-150页

作者： Xing Chang Xin He Jianying Li Zhenping Liu Ruizhen Pi Xuanxuan Luo Ruipeng Wang xiubao hu Sifan Lu Xianlong Zhang Maojun Wang National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhanChina

Centromere positioning and organization are crucial for genome evolution;however,research on centro-mere biology is largely influenced by the quality of available genome ***,we combined Oxford Nanopore and Pacific Biosciences technologies to de novo assemble two high-quality reference genomes for Gossypium hirsutum(TM-1)and Gossypium barbadense(3-79).Compared with previously published reference genomes,our assemblies show substantial improvements,with the contig N50 improved by 4.6-fold and 5.6-fold,respectively,and thus represent the most complete cotton genomes to *** high-quality reference genomes enable us to characterize 14 and 5 complete centromeric regions for *** and ***,*** data revealed that the centromeres of allotetraploid cotton are occupied by members of the centromeric repeat for maize(CRM)and Tekay long terminal repeat families,and the CRM family reshapes the centromere structure of the At subgenome after *** two intertwined families have driven the convergent evolution of centromeres between the two subgenomes,ensuring centromere function and genome *** addition,the reposi-tioning and high sequence divergence of centromeres between *** and *** have contributed to speciation and centromere *** study sheds light on centromere evolution in a sig-nificant crop and provides an alternative approach for exploring the evolution of polyploid plants.

关键词： genome assembly centromere architecture convergent evolution polyploidization

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长江江苏泰州段刀鲚耳石微化学指纹特征

生态学杂志 2024年第4期43卷 967-974页

作者：胡玉海姜涛刘洪波陈修报杨健南京农业大学无锡渔业学院江苏无锡214081 中国水产科学研究院淡水渔业研究中心渔业微化学实验室江苏无锡214081

为了掌握长江江苏泰州段刀鲚的生活史履历模式及了解此江段刀鲚资源现状,利用电子探针微区分析技术研究了禁捕后长江泰州段刀鲚耳石的锶(Sr)和钙(Ca)微化学特征。结果表明:所有个体的耳石均存在Sr/Ca比值小于3的低值区(对应淡水生境)和... 详细信息

为了掌握长江江苏泰州段刀鲚的生活史履历模式及了解此江段刀鲚资源现状,利用电子探针微区分析技术研究了禁捕后长江泰州段刀鲚耳石的锶(Sr)和钙(Ca)微化学特征。结果表明:所有个体的耳石均存在Sr/Ca比值小于3的低值区(对应淡水生境)和大于3的高值区(对应半咸水或海水生境)的波动变化,为典型的溯河洄游生境履历;根据淡水系数将泰州段洄游型刀鲚分为短期淡水依存型和长期淡水依存型;两者淡水系数的显著性差异说明这两种类型上溯刀鲚群体起源于长江不同区域的产卵场;结合性腺发育状态调查发现,泰州江段对于洄游型刀鲚来说主要起到洄游通道的生态功能作用。研究结果为了解长江十年禁渔对经济鱼类资源和生境恢复效果提供了最新的数据依据。

关键词：刀鲚耳石微化学长江江苏泰州段生境履历资源

EZH2 identifies the precursors of human natural killer cells with trained immunity

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Cancer Biology & Medicine 2021年第4期18卷 1021-1039页

作者： Chen Zhang Jie Yin Jian Zheng Jun Xiao Jiajian hu Yudong Su Kaichen Zhou Yingchi Zhang Xuzhen Zhang Hong Zhang Qian Sun Yang Wang Wenwen Yu Feng Wei Qiang Zhao Long Li xiubao Ren Department of Immunology and Biotherapy Key Laboratory of Cancer Immunology and BiotherapyTianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin’s Clinical Research Center for CancerTianjin 300060China Department of Immunology Key Laboratory of Immune Microenvironment and Disease of the Ministry of EducationTianjin Medical UniversityTianjin 300070China Department of Pediatric Cancer Tianjin Medical University Cancer Institute and HospitalTianjin 300060China State Key Laboratory of Experimental Hematology and Division of Pediatric Blood Diseases Center Institute of Hematology and Blood Diseases HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin 300000China Center for Stem Cell Medicine Chinese Academy of Medical SciencesBeijing 100730China Research Center of Basic Medicine Science Tianjin Medical UniversityTianjin 300070China

Objective:Trained immunity of natural killer(NK)cells has shown great potential in the treatment of cancers by eliciting enhanced effector responses to restimulation by cytokines or cancer cells for long time periods after ***,the human NK cells responsible for the generation and maintenance of trained immunity are largely *** hypothesized that heterogeneous human NK cells would respond differentially to stimulation with a combination of IL-12,IL-15,and IL-18,and that an NK cell subset might exist that is mainly responsible for the induction of trained *** the basis of our hypothesis,we aimed to identify the subset from which cytokine-trained human NK cells originate and to explore possible regulatory targets for drug ***:Flow cytometry assays were performed to analyze the functions of cytokine-trained NK cells and examine cell division and protein expression in NK cell ***-cell RNA sequencing(scRNA-seq)plus TotalSeq™technology was used to track the heterogeneity of NK cells during the induction of trained ***:Traditional developmental markers for peripheral NK cells were unable to identify the precursors of human NK cells with trained ***,we used scRNA-seq plus TotalSeq™technology to track the heterogeneity of NK cells during the induction of trained immunity and identified a unique cluster of CD57−NKG2A+EZH2+IFNG+MKI67+IL12R+IL15R+IL18R+NK *** and pseudotime trajectory analyses suggested that this cluster of NK cells contained the precursor of trained NK *** then used flow cytometry to further investigate the role of EZH2 in trained NK precursors and found that CD57−NKG2A+EZH2+NK cells had faster cell cycles and an enhanced trained phenotype,and EZH2 inhibition significantly impaired the induction of trained immunity in NK *** results suggested that EZH2 is a unique epigenetic marker of precursors of human NK cells with trained ***:Ou

关键词： Natural killer cells trained immunity precursor EZH2 cell cycle

基于耳石微化学分析的丹东鸭绿江刀鲚不同生态表型生境履历的研究

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海洋渔业 2023年第3期45卷 278-290页

作者：胡玉海姜涛刘洪波陈修报杨健南京农业大学无锡渔业学院江苏无锡214081 中国水产科学研究院淡水渔业研究中心渔业微化学实验室江苏无锡214081

为了解东北地区刀鲚(Coilia nasus)的生活史履历特征,2020年7月对丹东鸭绿江水域刀鲚进行调查,获得传统长颌鲚10尾、短颌鲚3尾,并首次采集到“杂颌鲚”6尾。通过电子探针微区分析技术对不同刀鲚进行了研究。耳石Sr和Ca定量线分析及Sr面... 详细信息

为了解东北地区刀鲚(Coilia nasus)的生活史履历特征,2020年7月对丹东鸭绿江水域刀鲚进行调查,获得传统长颌鲚10尾、短颌鲚3尾,并首次采集到“杂颌鲚”6尾。通过电子探针微区分析技术对不同刀鲚进行了研究。耳石Sr和Ca定量线分析及Sr面分布图谱分析结果显示,长颌鲚既有具江海洄游生境履历的生态表型,亦有仅具淡水定居生境履历的生态表型;而短颌鲚及“杂颌鲚”个体则全为河口孵化起源、具江海洄游生境履历的生态表型。结果表明,丹东鸭绿江刀鲚类型的组成非常复杂,生境履历极富多样性,显示其可能是一个独特资源地理种群。

关键词：鸭绿江刀鲚上颌骨生活史生境履历

Multiplexed imaging of tumor immune microenvironmental markers in locally advanced or metastatic non-small-cell lung cancer characterizes the features of response to PD-1 blockade plus chemotherapy

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Cancer Communications 2022年第12期42卷 1331-1346页

作者： Fengying Wu Tao Jiang Gongyan Chen Yunchao huang Jianying Zhou Lizhu Lin Jifeng Feng Zhehai Wang Yongqian Shu Jianhua Shi Yi hu Qiming Wang Ying Cheng Jianhua Chen Xiaoyan Lin Yongsheng Wang Jianan huang Jiuwei Cui Lejie Cao Yunpeng Liu Yiping Zhang Yueyin Pan Jun Zhao LiPing Wang Jianhua Chang Qun Chen xiubao Ren Wei Zhang Yun Fan Zhiyong He Jian Fang Kangsheng Gu Xiaorong Dong Tao Zhang Wei Shi Jianjun Zou Xuejuan Bai Shengxiang Ren Caicun Zhou Department of Thoracic Medical Oncology Shanghai Pulmonary HospitalTongji University School of MedicineShanghai 200433P.R.China Department of Respiratory Medicine Harbin Medical University Cancer HospitalHarbinHeilongjiang 150000P.R.China Department of Thoracic Surgery Oncology Yunnan Cancer Hospital&The Third Affiliated Hospital of Kunming Medical University&Yunnan Cancer CenterKunmingYunnan 650118P.R.China Department of Respiratory Medicine The First Affiliated Hospital of Zhejiang UniversityHangzhouZhejiang 310009P.R.China Department of Oncology Center The First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhouGuangdong 510405P.R.China Department of Thoracic Medical Oncology Jiangsu Cancer Hospital&Jiangsu Institute of Cancer Research&The Affiliated Cancer Hospital of Nanjing Medical UniversityNanjingJiangsu 210009P.R.China Department of Respiratory Shandong Cancer Hospital&InstituteJinanShandong 250117P.R.China Department of Oncology Jiangsu Province HospitalNanjingJiangsu 210000P.R.China Department of Medical Oncology Linyi Cancer HospitalLinyiShandong 276000P.R.China Department of Oncology Chinese PLA General HospitalBeijing 100853P.R.China Department of Respiratory Medicine Henan Cancer HospitalZhengzhouHenan 450000P.R.China Department of Thoracic Oncology Jilin Cancer HospitalChangchunJilin 130012P.R.China Department of Medical Oncology-Chest Hunan Cancer HospitalChangshaHunan 410006P.R.China Department of Oncology Fujian Medical University Union HospitalFuzhouFujian 350001P.R.China Department of Thoracic Medical Oncology West China HospitalSichuan UniversityChengduSichuan 610000P.R.China Department of Respiration First Affiliated Hospital of Suzhou UniversitySuzhouJiangsu 215002P.R.China Department of Medical Oncology The First Bethune Hospital of Jilin UniversityChangchunJilin 130021P.R.China Pulmonary and Critical Care Medicine The First Affiliated Hospital University of Science Technology of ChinaHefeiAnhui 230036P.R.China De

Background:Although programmed cell death 1(PD-1)blockade plus chemotherapy can significantly prolong the progression-free survival(PFS)and overall survival(OS)in first-line settings in patients with driver-negative advanced non-small-cell lung cancer(NSCLC),the predictive biomarkers remain ***,we investigated the predictive value of tumor immune microenvironmental marker expression to characterize the response features to PD-1 blockade plus ***:Tumor tissue samples at baseline were prospectively collected from 144 locally advanced or metastatic NSCLC patients without driver gene alterations who received camrelizumab plus chemotherapy or chemotherapy *** immune microenvironmental markers,including PD-1 ligand(PDL1),CD8,CD68,CD4 and forkhead box P3,were assessed using multiplex immunofluorescence(mIF)***-Meier curveswere used to determine treatment outcome differences according to their expression *** profiles were compared between tumors with distinct expression levels of these markers and their ***:Responders had significantly higher CD8/PD-L1(P=0.015)or CD68/PD-L1 co-expression levels(P=0.021)than non-responders in the camrelizumab plus chemotherapy group,while no difference was observed in the chemotherapy *** with high CD8/PD-L1 or CD68/PD-L1 co-expression level was associated with significantly longer PFS(P=0.002,P=0.024;respectively)and OS(P=0.006,P=0.026;respectively)than those with low co-expression in camrelizumab plus chemotherapy *** comparing survival in the camrelizumab plus chemotherapy with chemotherapy by CD8/PD-L1 co-expression stratification,significantly better PFS(P=0.003)and OS(P=0.032)were observed in high co-expression *** predictive value of CD8/PD-L1 and CD68/PD-L1 co-expression remained statistically significant for PFS and OS when adjusting clinicopathological *** the prevalence of TP53 or KRAS mutations was similar bet

关键词： Non-small-cell lung cancer PD-1 CD8 CD68 tumor immune microenvironment

经直肠超声造影､血清前列腺特异性抗原检测在前列腺癌穿刺活检诊断中的应用

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中国性科学 2022年第2期31卷 9-12页

作者：贺修宝李灿胡小梅郴州市第一人民医院超声科湖南郴州423000

目的研究经直肠超声造影(CE-TRUS)､血清前列腺特异性抗原(PSA)在前列腺穿刺活检诊断前列腺癌(PCa)中的应用价值。方法选取2018年1月至2020年9月郴州市第一人民医院诊治的85例PCa患者作为研究组,另选取同期85例前列腺良性增生患者作为对... 详细信息

目的研究经直肠超声造影(CE-TRUS)､血清前列腺特异性抗原(PSA)在前列腺穿刺活检诊断前列腺癌(PCa)中的应用价值。方法选取2018年1月至2020年9月郴州市第一人民医院诊治的85例PCa患者作为研究组,另选取同期85例前列腺良性增生患者作为对照组。比较两组前列腺穿刺活检､CE-TRUS阳性率､血清PSA水平,分析前列腺穿刺活检､CE-TRUS､血清PSA单独及联合诊断PCa的价值,对比研究组不同Gleason分级患者CE-TRUS参数[达峰时间(TIP)､峰值强度(PI)､曲线下面积(AUC)]､血清PSA水平,分析CE-TRUS参数､血清PSA水平与Gleason分级的相关性及对Gleason分级的评估价值。结果研究组前列腺穿刺活检､CE-TRUS阳性率､血清PSA水平高于对照组(P<0.05);前列腺穿刺活检､CE-TRUS､血清PSA联合诊断PCa的AUC最大,为0.942;CE-TRUS的PI､AUC及血清PSA与Gleason分级呈正相关(P<0.05);CE-TRUS的PI､AUC及血清PSA联合评估PCa Gleason分级≥4+3的AUC优于上述指标单一评估。结论CE-TRUS联合血清PSA有助于提高前列腺穿刺活检诊断PCa的价值,可为临床评估PCa恶性程度､拟定个性化治疗方案提供循证支持。

关键词：前列腺特异性抗原前列腺穿刺活检前列腺癌 Gleason分级

基于耳石微化学分析的鱼类种群生态学研究进展

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渔业科学进展 2022年第1期43卷 1-14页

作者：轩中亚姜涛刘洪波陈修报胡玉海杨健南京农业大学无锡渔业学院江苏无锡214081 中国水产科学研究院淡水渔业研究中心中国水产科学研究院内陆渔业生态环境与资源重点开放实验室江苏无锡214081

研究鱼类的种群生态学,破解其数量特征、生境利用、种群结构和动态变化等种群属性是保护和合理开发天然渔业资源的基础。耳石微化学分析是破解鱼类的种群生态学难题的新颖而有力的手段,不仅可以反演鱼类生活史过程中所经历的环境条件,... 详细信息

研究鱼类的种群生态学,破解其数量特征、生境利用、种群结构和动态变化等种群属性是保护和合理开发天然渔业资源的基础。耳石微化学分析是破解鱼类的种群生态学难题的新颖而有力的手段,不仅可以反演鱼类生活史过程中所经历的环境条件,还可溯源资源群起源的产卵场等关键生境,在鱼类资源种群空间结构形成机制研究及动态评估中具有优势。本文在分析耳石元素组成及其沉积特征的基础上,重点介绍了耳石微化学分析在鱼类种群生态学(如反演鱼类生活史、评估种群结构、把握关联性和破解混合群体的构成与来源等)中的应用进展,也客观评述了耳石微化学分析在鱼类种群生态学研究中的局限性与解决途径,并对今后相关技术的发展趋势及需要关注的科学问题进行了展望。

关键词：耳石微化学分析鱼类种群生态学生活史生境关联性

三亚蜈支洲岛海洋牧场近岛区底表大型底栖动物群落结构及评价

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热带海洋学报 2022年第3期41卷 135-146页

作者：马文刚夏景全魏一凡尹洪洋覃乐政刘相波胡雪晴许强李秀保王爱民海南大学海洋学院南海海洋资源利用国家重点实验室海南海口570228

三亚蜈支洲岛海洋牧场拥有丰富的岛礁生物资源。为掌握海洋牧场近岛区的底表大型底栖动物群落组成和分布特征及其影响因子、评估海洋牧场底栖生态系统健康状况,本研究于2020—2021年进行了底表大型无脊椎动物群落季节变动的调查。结果表... 详细信息

三亚蜈支洲岛海洋牧场拥有丰富的岛礁生物资源。为掌握海洋牧场近岛区的底表大型底栖动物群落组成和分布特征及其影响因子、评估海洋牧场底栖生态系统健康状况,本研究于2020—2021年进行了底表大型无脊椎动物群落季节变动的调查。结果表明:近岛珊瑚礁区共鉴定出棘皮动物、软体动物、节肢动物3大门类90种,其中秋季种类数最多为55种,夏季种类数最少为16种;底表大型底栖动物的年平均丰度为0.87±0.26ind.·m^(-2),年平均生物量为76.99±34.32g·m^(-2)。群落聚类分析(cluster)与多维排序尺度分析(multidimensional Scaling, MDS)表明,该区域群落结构季节性差异不显著,各站位间群落结构受沉积物性质以及人类活动的频繁程度的影响,形成北部与南部区域2个聚类组。全年的物种丰富度指数d为2.7±1.16,多样性指数H′为3.14±0.88,均匀度指数J为0.76±0.11。基于多样性指数H’及多变量海洋生物指数(Multivariate-AZTI′s marine biotic index, M-AMBI)评价指标,海洋牧场近岛区环境除夏季为轻度污染外,其他季节均为无污染状态。采用动物丰度与生物量比较曲线(abundance-biomass curves, ABC曲线)法评价底栖动物群落稳定性状况得出,除冬季以外,其他季节底表大型底栖动物群落受到一定程度干扰,尤其是夏季群落结构稳定性较低。建议应持续关注底表大型底栖动物群落变动,调整、优化涉海休闲旅游活动,以保证蜈支洲岛海洋牧场生态系统的长期健康与稳定。

关键词：海洋牧场珊瑚礁底表大型底栖动物群落结构环境因子

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