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MiR-1306-5p对鸡前脂肪细胞增殖与分化的影响

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农业生物技术学报 2025年第1期33卷 157-165页

作者：孙楠桢张蕾陈若楠张若彤孙杰石河子大学动物科技学院

miR-1306-5p对肿瘤细胞的增殖具有促进作用，对脂肪细胞增殖与分化的影响尚不明确。课题组前期采集玫瑰冠鸡(Gallus gallus)和科宝鸡胚胎期第8天胸肌组织进行高通量测序，本研究选择测序数据中筛选出的差异表达的miR-1306-5p，旨在探究m... 详细信息

miR-1306-5p对肿瘤细胞的增殖具有促进作用，对脂肪细胞增殖与分化的影响尚不明确。课题组前期采集玫瑰冠鸡(Gallus gallus)和科宝鸡胚胎期第8天胸肌组织进行高通量测序，本研究选择测序数据中筛选出的差异表达的miR-1306-5p，旨在探究miR-1306-5p在鸡前脂肪细胞增殖与分化过程中的功能。利用Targetscan和miRanda软件预测miR-1306-5p的靶基因，以脂肪代谢相关基因—骨形态发生蛋白2(bone morphogenetic protein 2, BMP2)为靶基因构建野生型和突变型载体，与miR-1306-5p模拟物共转染鸡成纤维细胞，利用荧光素酶报告基因检测系统验证二者的靶标关系。以miR-1306-5p模拟物和抑制物分别转染鸡前脂肪细胞，利用CCK-8(Cell Counting Kit-8)试剂盒检测细胞增殖能力，通过油红O染色检测油酸诱导的分化情况。结果显示，BMP2野生型与miR-1306-5p模拟物共转染组的荧光素酶活性较阴性对照极显著下降(P<0.01)，而突变型没有显著变化；miR-1306-5p模拟物转染鸡前脂肪细胞48、72和96h，细胞增殖能力极显著低于阴性对照(P<0.01)；在鸡前脂肪细胞的油酸诱导分化过程中，脂肪酸结合蛋白4(fatty acid binding protein 4, FABP4)基因表达量极显著升高(P<0.01)，而内源性miR-1306-5p和BMP2在诱导分化48和72 h的基因表达量极显著降低(P<0.01)；在鸡前脂肪细胞中转染miR-1306-5p模拟物后脂滴较对照小，转染miR-1306-5p抑制物后脂滴增多。因此，miR-1306-5p可能通过靶向BMP2而抑制鸡前脂肪细胞的分化。本研究为深入探讨miR-1306-5p在鸡前脂肪细胞分化中的作用机制提供基础资料。

关键词：鸡前脂肪细胞 miR-1306-5p 骨形态发生蛋白2(BMP2) 细胞增殖细胞分化

Rapamycin liposome gutta inhibiting fungal keratitis of rats

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International Journal of Ophthalmology(English edition) 2019年第4期12卷 536-541页

作者： zhen-Hua Zhang Feng Teng Qing-xiu sun Shu-zhen Wang Chao Liu Gui-Qiu Zhao Department of Ophthalmology Qingdao Central Hospital the Second Clinical Hospital of Qingdao University Department of Ophthalmology the Affiliated Hospital of Qingdao University

AIM: To study the therapeutic effect of rapamycin liposome eyedrops on fungal keratitis(FK) and its effect on the expression of monocyte chemotactic protein-1(MCP-1).METHODS: This study adopted the thin film dispersion method to prepare rapamycin liposomes eyedrops, as well as used the orthogonal design to analyze and study main influencing factors that affected the quality of liposomes. Totally 96 healthy Wistar rats were randomly divided into four groups: normal control group(A), FK blank control group(B), FK blank liposomes control group(C), and 30 FK rapamycin liposome treatment group(D). Groups B, C, and D were first prepared as FK animal models. The corneal response was recorded in details on day 1, 3, 5, 7, and 14 after modeling. Six rats were obtained and immunohistochemistry and semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) were used to detect the expression of MCP-1 protein and mRNA, ***: The severity of corneal lesions in the rapamycin treatment group was reduced, and the clinical score of the slit lamp examination was lower than that of Groups B and C(P<0.01). The expression of MCP-1 in rapamycin treatment group was significantly inhibited, comparing to that of groups B and C(P<0.01).CONCLUSION: Liposome is a good drug carrier for rapamycin. Rapamycin has a good therapeutic effect on FK. It can reduce FK fungal burden and significantly inhibit the expression of MCP-1 protein and mRNA.

关键词： fungal keratitis rapamycin liposome monocyte chemotactic protein-1 rats

Ionic liquid-based salting-out extraction of bio-chemicals

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Chinese Journal of Chemical Engineering 2021年第2期34卷 185-193页

作者： Jianying Dai Yaqin sun Zhilong xiu School of Bioengineering Dalian University of TechnologyDalian 116024China

Ionic liquids(ILs)are known as green solvents,and have been widely used in the dissolution and transformation of biopolymers,the extraction of bioactive compounds and metal ions,and the capture of SO2 or ***,less attention was given to the separation of bio-based chemicals,such as diols and organic ***-based chemicals can be efficiently separated by organic solvent-based salting-out extraction(SOE)from fermentation broths,while organic solvents are normally unfriendly to environment and process safety in commercialized production due to their toxicity or/and *** recent years,the IL-based SOE system has been explored in the separation of bio-based chemicals as an alternative of organic solvent-based SOE *** this review,the progress of IL-based SOE of biobased chemicals has been summarized,including the effect of ILs structure on the formation of aqueous two phases,and the influences of ILs structure and concentration,temperature and pH on the partition behaviors of target products and ILs as well as removal of *** of bio-based chemicals could be distributed into the IL-rich phase with high recovery,while the partition behaviors of bio-based chemicals are sometimes different from that in organic solvent-based SOE *** the results of ILbased SOE are promising,further studies are still required in the increased selectivity of target products over by-products,recovery and recycling of ILs,and the separation between ILs and bio-based ***,three kinds of integrated bioprocesses would be developed on basis of utilization of ILs as extractant for SOE,catalyst for condensation reaction and solvent for pretreatment of lignocellulose.

关键词： Downstream processing Bioseparation Ionic liquids Salting-out extraction Partition behavior Bio-based chemicals

Surface embedded binary Co-Cu hydroxy phosphate over surface of oxy/hydroxides for efficient nitrate reduction to ammonia

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Nano Research 2025年第1期18卷 195-207页

作者： Chun Zhu Yifei Yang Yike Jing zhen Zeng Xuesong Yi Wei sun Fei Yang Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province School of Environmental Science and EngineeringHainan UniversityHaikou 570228China

Electrochemical nitrate reduction reaction(NO3RR)to ammonia is a promising approach to address excess Ncontamination in aqueous environment but suffers from a limited ammonia yield and selectivity due to the low concentration and sluggish *** work reports a three-dimensional porous electrode with binary Co_(2)Cu_(1)hydroxy phosphate embedded into the surface of Ni_(3)Co_(1)oxy/hydroxides(CoCu-PH@NiCo/Ni,“PH”means phosphate-hydroxide),which delivers a yield of 4.13 mg·h^(-1)·cm^(-2)with a high Faraday efficiency of 92.6%under a low nitrate concentration of 40 mM and provide an impressive *** embedded structure of the as-prepared catalyst exhibits unique metal coordination ligands and valence states different from the unitary *** electrochemical in situ infrared spectra and theoretical calculations revealed that the Co sites with the ability to dissociate water molecular to form adsorbed OH*can effectively provide the required protons for the deoxygenation and hydrogenation steps during NO3-conversion,thereby,effectively reducing the energy barrier of the key transition states in the NO3RR process to improve the intrinsic *** demonstrate that the rational design of binary components with the appropriate ability to activate water molecules as well as the threedimensional porous skeletal structure are expected to promote effectively the low-concentration nitrates conversion to ammonia.

关键词： nitrate reduction hydroxy phosphate surface embedded water dissociation active hydroxy adsorbed

Identification of microRNAs and messenger RNAs involved in human umbilical cord mesenchymal stem cell treatment of ischemic cerebral infarction using integrated bioinformatics analysis

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Neural Regeneration Research 2019年第9期14卷 1610-1616页

作者： Yin-Meng Qu Xin sun xiu-Li Yan Hang Jin zhen-Ni Guo Yi Yang Stroke Center Neuroscience CenterDepartment of Neurologythe First Hospital of Jilin UniversityChangchunJilin ProvinceChina Clinical Trial and Research Center for Stroke Department of Neurologythe First Hospital of Jilin UniversityChangchunJilin ProvinceChina

In recent years,a large number of differentially expressed genes have been identified in human umbilical cord mesenchymal stem cell(hUMSC)transplants for the treatment of ischemic cerebral *** genes are involved in various biochemical processes,but the role of microRNAs(miRNAs)in this process is still *** the Gene Expression Omnibus(GEO)database,we downloaded two microarray datasets for GSE78731(messenger RNA(mRNA)profile)and GSE97532(miRNA profile).The differentially expressed genes screened were compared between the hUMSC group and the middle cerebral artery occlusion *** ontology enrichment and pathway enrichment analyses were subsequently conducted using the online Database for Annotation,Visualization,and Integrated *** genes were applied to perform weighted gene co-suppression analyses,to establish a weighted co-expression network ***,the protein-protein interaction network for differentially expressed genes from turquoise modules was built using Cytoscape(version 3.40)and the most highly correlated subnetwork was extracted from the protein-protein interaction network using the MCODE *** predicted target genes for differentially expressed miRNAs were also identified using the online database starBase v3.0.A total of 3698 differentially expressed genes were *** ontology analysis demonstrated that differentially expressed genes that are related to hUMSC treatment of ischemic cerebral infarction are involved in endocytosis and inflammatory *** identified 12 differentially expressed miRNAs in middle cerebral artery occlusion rats after hUMSC treatment,and these differentially expressed miRNAs were mainly involved in signaling in inflammatory pathways,such as in the regulation of neutrophil *** conclusion,we have identified a number of differentially expressed genes and differentially expressed mRNAs,miRNA-mRNAs,and signaling pathways involved in the hUMSC treatment of

关键词： nerve regeneration ischemic cerebral infarction human umbilical cord mesenchymal stem cell treatment bioinformatics analysis differentially expressed genes differentially expressed mRNAs inflammatory response stem cell therapy weighted gene co-suppression analysis WGCNA protein-protein interaction network PPI hUMSC neural regeneration

Corotational nonlinear analyses of laminated shell structures using a 4-node quadrilateral flat shell element with drilling stiffness

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Acta Mechanica Sinica 2014年第3期30卷 418-429页

作者： zhen Wang Qin sun School of Aeronautics Northwestern Polytechnical University

A new 4-node quadrilateral flat shell element is developed for geometrically nonlinear analyses of thin and moderately thick laminated shell structures. The fiat shell element is constructed by combining a quadrilateral area co- ordinate method （QAC） based membrane element AGQ6- II, and a Timoshenko beam function （TBF） method based shear deformable plate bending element ARS-Q12. In order to model folded plates and connect with beam elements, the drilling stiffness is added to the element stiffness matrix based on the mixed variational principle. The transverse shear rigidity matrix, based on the first-order shear deformation theory （FSDT）, for the laminated composite plate is evaluated using the transverse equilibrium conditions, while the shear correction factors are not needed. The conventional TBF methods are also modified to efficiently calculate the element stiffness for laminate. The new shell element is extended to large deflection and post-buckling analyses of isotropic and laminated composite shells based on the element independent corotational formulation. Numerical re- sults show that the present shell element has an excellent numerical performance for the test examples, and is applicable to stiffened plates.

关键词： Geometrically nonlinear. Quadrilateral area co-ordinate method Timoshenko beam function Laminatedcomposite Drilling degree of freedom ~ Corotational for-mulation

Advances in Research on Anticancer Properties of Salidroside

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Chinese Journal of Integrative Medicine 2021年第2期27卷 153-160页

作者： sun An-qi JU xiu-lian Key Laboratory for Green Chemical Process of Ministry of Education School of Chemical Engineering&PharmacyWuhan Institute of TechnologyWuhan(430073)China The College of Post and Telecommunication Wuhan Institute of TechnologyWuhan(430073)China

Salidroside is a phenolic secondary metabolite present in plants of the genus Rhodiola,and studies investigating its extensive pharmacological activities and mechanisms have recently attracted increasing *** review summarizes the progress of recent research on the antiproliferative activities of salidroside and its effects on breast,ovarian,cervical,colorectal,lung,liver,gastric,bladder,renal,and skin cancer as well as gliomas and ***,it provides a reference for the further development and utilization of salidroside.

关键词： salidroside anticancer mechanism antiproliferative Chinese medicine review

Ability of Helicobacter pylori to internalize into Candida

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World Journal of Gastroenterology 2024年第16期30卷 2281-2284页

作者： zheng-Hong Chen Jian-Chao sun Ting-xiu Yang Gu-zhen Cui Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou Guizhou Medical UniversityGuiyang 561113Guizhou ProvinceChina Key Laboratory of Endemic and Ethnic Diseases of Ministry of Education Guizhou Medical UniversityGuiyang 561113Guizhou ProvinceChina Joint Laboratory of Helicobacter Pylori and Intestinal Microecology Affiliated Hospital of Guizhou Medical UniversityGuiyang 550009Guizhou ProvinceChina Department of Quality Control Guizhou Provincial Center for Clinical LaboratoryGuiyang 550002Guizhou ProvinceChina Department of Hospital Infection and Management Guizhou Provincial People’s HospitalGuiyang 550002Guizhou ProvinceChina

The following are our views regarding the“letter to the editor”(Helicobacter is preserved in yeast vacuoles!Does Koch’s postulates confirm it?)by Alipour and Gaeini,and the response“letter to the editor”(Candida accommodates nonculturable Helicobacter pylori in its vacuole-Koch’s postulates aren’t applicable)by Siavoshi and *** and Gaeini rejected the methods,results,discussion,and conclusions summarized in a review article by Siavoshi and *** present article reviews and discusses evidence on the evolutionary adaptation of Helicobacter pylori(***)to thrive in Candida cell vacuoles and concludes that Candida could act as a Trojan horse,transporting potentially infectious *** into the stomach of humans.

关键词： Helicobacter pylori Candida yeast Intracellular presence Helicobacter pylori-specific gene Helicobacter pylori transmission

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食用牛油的制备及深加工技术综述

粮油食品科技 2017年第5期25卷 32-36页

作者：王家升张慧丁秀臻孙思远赵飞李向阳山东农业大学食品科学与工程学院山东泰安271018

牛油及牛油精深加工产品因其具有良好的稳定性、持气性及特有的纯正风味被广泛应用于食品加工业。作为主要的食用动物油脂,牛油越来越受到人们的关注。从牛油的理化特性、化学成分,现有牛油制备、精深加工技术及其应用进行综述,旨在为... 详细信息

牛油及牛油精深加工产品因其具有良好的稳定性、持气性及特有的纯正风味被广泛应用于食品加工业。作为主要的食用动物油脂,牛油越来越受到人们的关注。从牛油的理化特性、化学成分,现有牛油制备、精深加工技术及其应用进行综述,旨在为牛油产品的进一步开发与利用提供依据。

关键词：牛油理化特性精深加工应用综述