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Expression of hepatitis B virus 1.3-fold genome plasmid in an SV40 T-antigen-immortalized mouse hepatic cell line

World Journal of Gastroenterology 2013年第44期19卷 8020-8027页

作者： xiu-guang song Peng-Fei Bian Shu-Li Yu xiu-Hua Zhao Wei Xu Xue-Hui Bu Xia Li Li-Xian Ma Department of Infectious Diseases Qilu Hospital Shandong University Jinan Infectious Disease Hospital Shandong University Laboratory for Tumor Immunity and Traditional Chinese Drug Immunity Institute of Basic Medicine Shandong Academy of Medical Science School of Medicine Shandong University

AIM:To investigate the expression of the hepatitis B virus(HBV)1.3-fold genome plasmid(pHBV1.3)in an immortalized mouse hepatic cell line induced by SV40T-antigen(SV40T)***:Mouse hepatic cells were isolated from mouse liver tissue fragments from 3-5 d old Kunming mice by the direct collagenase digestion method and cultured in *** pRSV-T plasmid was transfected into mouse hepatic cells to establish an SV40LT-immortalized mouse hepatic cell *** SV40LT-immortalized mouse hepatic cells were identified and transfected with the pHBV1.3 *** levels of hepatitis B surface antigen(HBsAg)and hepatitis B e antigen(HBeAg)in the supernatant were determined by an electrochemiluminescence immunoassay at 24,48,72 and 96 h after *** expressions of HBsAg and hepatitis B c antigen(HBcAg)in the cells were investigated by indirect immunofluorescence *** presence of HBV DNA replication intermediates in the transfected cells and viral particles in the supernatant of the transfected cell cultures was monitored using the Southern hybridization assay and transmission electronic microscopy,***:The pRSV-T plasmid was used to immortalize mouse hepatocytes and an SV40LT-immortalized mouse hepatic cell line was successfully ***40LT-immortalized mouse hepatic cells have the same morphology and growth characteristics as primary mouse hepatic cells can be subcultured and produce albumin and cytokeratin-18 in *** mouse hepatic cells did not show the characteristics of tumor cells,as alpha-fetoprotein levels were comparable(0.58±0.37 vs 0.61±0.31,P=0.37).SV40LTimmortalized mouse hepatic cells were then transfected with the pHBV1.3 plasmid,and it was found that the HBV genome replicated in SV40LT-immortalized mouse hepatic *** levels of HBsAg and HBeAg continuously increased in the supernatant after the transfection of pHBV1.3,and began to decrease 72 h after *** expressions of HBsAg and

关键词： SV40 T-antigen Mouse hepatic cell Hepatitis B virus 1.3-fold genome plasmids Immortalized Liposomes Transfection

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Separation of the ionospheric and thermospheric contributions in the data from the Triple Ionosophere Photo Meter onboard the Feng-Yun 3E satellite

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Science China Earth Sciences 2025年第1期68卷 172-184页

作者： Fang JIANG Tian MAO Jin-song WANG xiu-Qing HU Xiao-Xin ZHANG Liping FU Nan JIA Tianfang WANG National Space Science Center Chinese Academy of Sciences Beijing Key Laboratory of Space Environment Exploration Key Laboratory of Environmental Space Situation Awareness Technology National Satellite Meteorological Center China Meteorological Administration

The Triple Ionosphere Photo Meter （TRIPM） is a far ultraviolet nadir-view photometer that is onboard the secondgeneration,polar-orbiting Chinese meteorological satellite Feng-Yun 3E （FY-3E）,which was launched on July 5,*** has three channels,and each is used to measure different types of emissions:（1） 135.6 nm airglow produced by the recombination of the O+ions and electrons in the absence of sunlight （nighttime channel）;（2） 135.6 nm airglow from the OI when sunlight is present （daytime/dusk-dawn 135.6 nm channel）;and （3） Airglow in the N2LBH of the TRIPM （daytime/dusk-dawn N2LBH channel）.FY-3E is a satellite operating in the early morning ***,the data are mostly distributed on the dawndusk side or at times when the solar zenith angle is relatively *** this study,combined with simulation calculations,the data are *** further explore the application value of the data products,a model is used to separate the ionospheric radiative recombination contribution from the thermosphere photoelectron impact excitation contribution in the data from the daytime/dusk-dawn 135.6 nm *** approach not only improves the accuracy of O/N2derived from the 135.6 nm channel and N2LBH channel but also provides the ionospheric 135.6 nm radiance at the same time as the *** results show that the 135.6 nm radiance originating from the combination of O+and electrons extracted from the measurement data exhibits similar equatorial anomaly peak structure characteristics and seasonal variations as the corresponding GPS total electron content （TEC） *** the other hand,the separated 135.6 nm radiance generated from the thermosphere photoelectron collision excitation does not contain radiative recombination contributions;thus,the ionosphere influence is greatly avoided when the O/N2is obtained.

关键词： Far ultraviolet 135.6 nm emission intensity Photoelectron impact excitation Radiative recombination excitation

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东方传统体育健身使命新说

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体育学刊 2004年第4期11卷 59-61页

作者：张永军杨秀清宋光春李鸣晓刘瑛山东理工大学体育系山东济南255049

结合新时代提出的多维健康观和生命观,对以"形神兼练"、"性命双修"为核心,以呼吸、意念及肢体运动的完美配合为基本特征的东方传统体育进行分析,统计中国历代帝王和道教相关人物的寿命,说明以强调人类肉体与精神统一、"天人合一"为指导... 详细信息

结合新时代提出的多维健康观和生命观,对以"形神兼练"、"性命双修"为核心,以呼吸、意念及肢体运动的完美配合为基本特征的东方传统体育进行分析,统计中国历代帝王和道教相关人物的寿命,说明以强调人类肉体与精神统一、"天人合一"为指导思想的传统体育锻炼方法在人体健康方面有着显著效果,面对日益高涨的通过体育促进健康、关爱生命的大众体育健身热潮,东方传统体育必将以独特的方式对人类的健康长寿发挥更大的作用。

关键词：东方传统体育健康长寿保健养生锻炼方法

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Clinical observation of white eye distribution in patients with hyperlipidemia: an artificial intelligence digital visual examination technique

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Journal of Traditional Chinese Medicine 2025年

作者： song Tianli LI Haixia LIU Li'an Department of Cardiology， the guang'anmen Hospital， China Academy of Chinese Medical Sciences National College， Beijing University of Chinese Medicine

Objective: To explore the clinical observation of white eye distribution characteristics of hyperlipidemic patients based on artificial intelligence digital eye diagnosis technology. Methods: One hundred and fifty subjects were examined in the outpatient and inpatient departments of guang'anmen Hospital of the China Academy of Traditional Chinese Medicine from 01 February 2022 to 01 February 2023， including 80 cases in the hyperlipidemic patient (HLP) group and 70 cases in the normal lipid level patient (NC) group. The two groups were collected and extracted by the artificial intelligence visual diagnostic instrument and analyzed by the MyEyeD-10 white eye shadowless imaging health intelligence analysis system. Finally， SPSS 26.0 (Version X; IBM Corp.， Armonk， NY， USA) was used for statistical processing. Results: Significant differences were noted in the scores of "spot" and "foggy" features between the two groups. Between groups， the "spot" feature score of the white eye morphology in the HLP group (11.07 ± 3.22) was higher than that in the NC group (7.50 ± 4.11) (P < 0.01). Moreover， the "foggy" feature score of the eye morphology in the HLP group (8.37 ± 2.25) was higher than that in the NC group (P < 0.01)， higher than that of the NC group (5.72 ± 1.21) (P < 0.05). There were significant differences in the "A" (stomach)， "B"， "O" (spleen)， and "M" (liver) eye-contact region scores between the two groups， and the "B"， "O" (spleen) and "M" (liver) eye-contact region scores were significantly different. The scores of the white eye channel region in the HLP group were significantly higher than those in the control group， with the "A"， "B"， and "O" regions (P < 0.01)， "M" region (P < 0.01)， "A"， "B"， "O"， and "M" region (P < 0.01). "M" zone (P < 0.05). The scores of "dull red" and "yellow" features were significantly different， and the scores of "dull red" and "yellow" colors of the white eye choroid in the HLP group were significantly higher than those in the HLP g

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数字生态赋能农业新质生产力培育的制度组态——基于必要条件分析法与动态定性比较分析法

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中国流通经济 2025年第1期39卷 115-126页

作者：罗光强宋新宇湖南农业大学经济学院湖南长沙410128

在百年未有之大变局下,人类正在迎来以数字技术为代表的新一轮科技革命和产业变革,形成以数字基础、数字政府、数字社会、数字经济、数字能力为内容的数字生态。数字生态不断催生新技术、新产业和新动能,促进新质生产力发展,推动新质生... 详细信息

在百年未有之大变局下,人类正在迎来以数字技术为代表的新一轮科技革命和产业变革,形成以数字基础、数字政府、数字社会、数字经济、数字能力为内容的数字生态。数字生态不断催生新技术、新产业和新动能,促进新质生产力发展,推动新质生产关系形成。当前,我国农业正面临着传统与现代并存二元结构转型升级关键期,必须充分发挥数字技术破解农业迂回生产分工难和产业组织集聚难的作用,以数字生态及与之相适配的制度组态推动农业新质生产力衍生与发展。基于此,以全国统一大市场建设为契机,运用生产力与生产关系的辩证关系原理,构建制度组态、数字生态与农业新质生产力培育的理论机制,综合运用必要条件分析法与动态定性比较分析法,利用我国30个省份(不包括西藏和港澳台地区)2019—2022年面板数据进行案例分析。定性比较分析法的单个必要性分析表明,数字生态要素的有效协同是培育高农业新质生产力的必要条件;必要条件分析表明,低数字能力与低数字经济制约农业新质生产力的培育;定性比较分析的条件组态充分性分析表明,培育农业新质生产力必须依靠数字生态要素及其匹配制度要素的有效组合,实施政府主导型、政府-市场双轮驱动型和政府-市场-社会三轮驱动型的组态路径。进一步加快农业新质生产力发展,应充分发挥全国统一大市场建设的数字化赋能作用,因地制宜选择农业新质生产力培育的组态路径,着力推动工业颠覆性技术在农业领域的广泛应用,大力加强农业数字化与数字化农业创新能力建设,全面深化以新质生产关系为导向的农业体制改革。

关键词：全国统一大市场农业新质生产力制度组态数字生态

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In situ observation and modeling of austenite grain growth in a Nb-Ti-bearing high carbon steel

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Journal of Iron and Steel Research International 2019年第3期26卷 292-300页

作者： guang Ji xiu-hua Gao Zhen-guang Liu Ke Zhang State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819LiaoningChina Jiangsu Shagang Group Huaigang Special Steel Co. Ltd.Huaian 223002JiangsuChina School of Materials Science and Engineering Jiangsu University of Science and TechnologyZhenjiang 212003JiangsuChina Institute of Research of Iron and Steel ShasteelZhangjiagang 215625JiangsuChina

In situ measurements of austenite grain growth were made at various temperatures in the range of 1273-1473 K with subsequent isothermal holding time of 3600 s for the Nb-Ti-bearing and Nb-Ti-free high carb on steel by using a confocal laser scanning *** solid solute behavior of Nb-Ti carbides during austenitizing process was *** experimental results indicate that the austenite grains of both steels grow up gradually with increasing the heating temperature and holding time;the size and growth rate of austenite grain of Nb-Ti-bearing high carbon steel are much lower than those of Nb-Ti-free high carbon steel.A large amount of(Nb,Ti)(C,N)nanoparticles are observed in Nb-Tibearing steel,which retain the strong pinning effect on austenite grain *** kinetics model of austenite grain growth of Nb-Ti-bearing steel during isothermal heat treatment is obtained and the predicted values calculated by using the model meet the experimental values very well.

关键词： Con focal laser scanning microscope Nb-Ti microalloying High carb on steel Grain growth Nanoparticle Grain growth model

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A review of the strengthening–toughening behavior and mechanisms of advanced structural materials by multifield coupling treatment

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International Journal of Minerals,Metallurgy and Materials 2022年第2期29卷 185-199页

作者： xiu song Lei Wang Yang Liu The Key Laboratory for Anisotropy and Texture of Materials(Ministry of Education) School of Materials Science and EngineeringNortheastern UniversityShenyang 110819China

The application of an external field is a promising method to control the microstructure of materials, leading to their improved performance. In the present paper, the strengthening and toughening behavior of some typical high-performance structural materials subjected to multifield coupling treatment, including electrostatic field, electro-pulse current, thermal field, and stress field, are reviewed in detail. In addition to the general observation that the plasticity of materials could be increased by multi-external fields, strength enhancement can be achieved by controlling atomic diffusion or phase transformations. The paper is not limited to the strengthening and toughening mechanisms of the multifield coupling effects on different types of structural materials but is intended to provide a generic method to improve both the strength and ductility of the materials. Finally, the prospects of the applications of multi-external fields have also been proposed based on current works.

关键词： multifield coupling structural materials strengthening toughening microstructure

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水泥搅拌桩桩土应力比研究

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山东大学学报（工学版） 2003年第4期33卷 467-469页

作者：宋修广宋文义苏公灿山东大学土建与水利学院山东济南250061 中铁十四局集团第一工程公司山东日照276826 济南市园林开发建设集团

桩土应力比是反映搅拌桩复合地基工作状态的一个重要参数 ,也是计算复合地基承载力和沉降的重要指标 .本文通过实测水泥搅拌桩复合地基的桩土应力比 ,分析了影响桩土应力比的主要因素 .在充分考虑桩土相互作用的基础上 ,提出了桩土应力... 详细信息

桩土应力比是反映搅拌桩复合地基工作状态的一个重要参数 ,也是计算复合地基承载力和沉降的重要指标 .本文通过实测水泥搅拌桩复合地基的桩土应力比 ,分析了影响桩土应力比的主要因素 .在充分考虑桩土相互作用的基础上 ,提出了桩土应力比改进计算公式 .公式计算简单 ,具有工程实用价值 .

关键词：水泥搅拌桩桩土应力比复合地基桩土应力比影响因素

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Current research on electrospinning of silk fibroin and its blends with natural and synthetic biodegradable polymers

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Frontiers of Materials Science 2013年第2期7卷 129-142页

作者： Jian-guang ZHANG xiu-Mei MO Biomaterials and Tissue Engineering Lab College of Chemistry Chemical Engineering & Biotechnology Donghua University Shanghai 201620 China

Silk fibroin （SF） is a kind of natural polymers with a great potential in biomedical application. Due to its good biocompatibility, biodegradability and minimal inflammatory reaction, SF is an excellent candidate for generating tissue engineering scaffolds. Electrospinning is a simple and effective method to fabricate nanofibers, which has several amazing characteristics such as very large surface area to volume ratio, flexibility in surface functionalities, and superior mechanical performance. The electro- spun nanofibers from SF and its blends have been used for varied tissue engineering. This paper will give a brief review about the structure, properties and applications of SF and blend nanofibers via electrospinning.

关键词： electrospinning nanofiber silk fibroin (SF) biodegradable polymer

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Reversing effect of Tanshinone on malignant phenotypes of human hepatocarcinoma cell line

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World Journal of Gastroenterology 1998年第4期4卷 45-47页

作者： YUAN Shu-Lan HUANG Ren-Min WANG xiu-Jie song Yi HUANG guang-Qi Institute of Cancer Research West China University of Medical Sciences Chengdu 610041Sichuan Province China

AIM To study the reversing effect of Chinese drug tanshinone on malignant phenotype of cancer *** Human hepatocarcinoma cell line (SMMC-7721) was treated in vitro with 0.5mg/L tanshinone for 4 days, and variation in cell differentiation was *** The morphology of cancer cells was tended toward well differentiation and cell growth was markedly inhibited. BrdU uptake assay and immunohistochemical stain of PCNA showed that the BrdU labeling rate and PCNA positive rate were lower than the controls, but no difference was found statistically as compared with all transretinoic acid. Flow cytometric assay demonstrated that S phase cells decreased and G0/G1 phase cells increased. Expression of c-myc oncogene protein decreased but the c-fos oncogene protein markedly *** Tanshinone could reverse the inducing differentiation in human hepatocarcinoma cells (SMMC-7721). It may become a new prospective inducer of cell differentiation to treat cancers.

关键词： tanshinone liver neoplasms carcinoma,hepatocellular tumor cell,cultured cell line immunohistochemistry proliferation cell nuclear antigen flow cytometry

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