检索结果-南通市图书馆

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

引用

Neural Regeneration Research 2025年第3期20卷 900-912页

作者： Baoqi Hu Rui wang Hanyue Zhang xiou wang Sijia Zhou Bo Ma Yan Luan Xin wang Xinlin Chen Zhichao Zhang Qianyan Kang Department of Ophthalmology The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi ProvinceChina Department of Ophthalmology The First Affiliated Hospital of Northwest UniversityXi’anShaanxi ProvinceChina Institute of Neurobiology Xi’an Jiaotong University Health Science CenterXi’anShaanxi ProvinceChina

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell ***,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development ***,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo *** this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in ***,we used SOX2 and PAX6 immunostaining to identify retinal progenitor *** then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,***-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between ***,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,*** timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant *** study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina *** findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

关键词： bipolar cells differentiation in vivo microglia Müller glia organotypic retinal explant culture postnatal retina development proliferation retinal progenitor cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

AR mutations in 28 patients with androgen insensitivity syndrome(Prader grade 0–3)

引用

Science China(Life Sciences) 2017年第7期60卷 700-706页

作者： Yi wang Chunxiu Gong xiou wang Miao Qin Beo'ing Key Laboratory for Genetics of Birth Defects Center of Endocrinology Genetics and Metabolism Beo'ing Children's Hospital The Capital Medical University National center for children's health Beijing 100045 China

We investigated the androgen receptor(AR) gene mutation profiles of Chinese patients exhibiting severe androgen insensitivity syndrome(AIS) phenotypes. The present study enrolled 28 patients with genetically diagnosed AIS, who presented with severe phenotypes(Prader grade 0–3). Patients and some family members were screened via amplification and sequencing of their AR exons 1–8, including the corresponding intronic flanking regions. Luteinizing(LH), follicle-stimulating(FSH), and testosterone(T) hormone levels were found to be slightly, but not significantly, higher in patients with complete androgen insensitivity syndrome(CAIS) than in patients with partial androgen insensitivity syndrome(PAIS)(P>0.05). We identified 24 different AR mutations, including 12 that were novel. Ten patients(cases 2, 3, 10, 28, 11, 12, 19, 20, 24, and 25) were found to carry five recurrent mutations(p.Y572 S, p.P914 S, p.S176 R, p.Y782 N, and p.R841H); of these, p.Y572 S, p.S176 R, and p.Y782 N were novel. Among the mutations identified in patients with CAIS, six(66.7%) were characterized as single-nucleotide missense mutations, and six(66.7%) were found to be located in the AR ligand-binding domain(LBD). Among the mutations identified in patients with PAIS, 15(93.8%) were found to be missense, and 11(68.8%) were found to be located in the LBD. Patients 10 and 28 were determined to harbor the same missense mutation(p.P914S), but were diagnosed with CAIS and PAIS, *** hormone levels were slightly, but not significantly, elevated in patients with CAIS compared to those with PAIS. Missense mutations spanning AR exons 1–8 were the predominant form of identified mutations, and these were mostly located in the AR LBD. Approximately 50% of the identified mutations were novel, and have enriched the AR gene-mutation database. Patients harboring identical mutations were in some instances found to exhibit divergent phenotypes.

关键词：雄激素受体突变谱综合征 AR 敏感 CAIS 基因诊断促黄体生成素

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

医院声环境和静音设计方法研究

引用

噪声与振动控制 2010年第3期30卷 113-117页

作者：李英王希鸥北京建筑工程学院建筑与城市规划学院北京100044

医院建筑特别是病房对安静要求比较高。嘈杂的声环境直接或间接的影响病人的身心健康,影响医生的工作质量。然而,目前许多医院存在比较严重的噪声污染,医院声环境的设计和研究并未引起足够的重视。以北京某三级甲等医院为例,通过实测医... 详细信息

医院建筑特别是病房对安静要求比较高。嘈杂的声环境直接或间接的影响病人的身心健康,影响医生的工作质量。然而,目前许多医院存在比较严重的噪声污染,医院声环境的设计和研究并未引起足够的重视。以北京某三级甲等医院为例,通过实测医院内的声环境,研究分析了医院噪声污染情况和声环境设计中的问题,并探讨了医院静音设计的方法。

关键词：声学医院声环境静音设计噪声

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

对团体操审美价值的探讨

引用

浙江体育科学 2000年第5期22卷 15-16页

作者：王秀梅辽宁师范大学体育学院!辽宁大连116029

从审美角度来分析团体操 ,重点阐述团体操中审美教育的价值 ,舞蹈美、体育美的价值和培养以及提高人们对团体操的审美能力 ,激发人们对美好生活的热爱

关键词：团体操审美价值

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Seasonal Predictability of the ensemble seasonal prediction by tier-1 and tier-1.5 models of ECMWF-UH

Seasonal Predictability of the ensemble seasonal prediction ...

引用

中国气象学会2006年年会“气候变化及其机理和模拟”分...

作者： Li Tao, Bing wang*, xiou-Hua Fu* (Nanjing University of Information Science and Technology, Nanjing, 210044)*IPRC, University of Hawaii at Manoa, Hawaii, USA

By using a hybrid model tier-1 that is coupled in the Indo-Pacific tropical ocean, we perform a set of 10 ensemble runs with different initial condition for two-season period that starts from November

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

极性晶体膜中束缚极化子的有效质量

引用

原子与分子物理学报 2007年第6期24卷 1189-1193页

作者：王秀清肖景林内蒙古民族大学物理与机电学院通辽028043

采用线性组合算符法和变分法,讨论了极性晶体膜中电子与SO声子耦合强,与LO声子耦合弱的电子-声子相互作用系统的有效质量.得出了束缚极化子的有效质量随膜厚的变化规律.对KCl材料进行数值计算的结果表明,不同支声子与电子相互作用对有... 详细信息

采用线性组合算符法和变分法,讨论了极性晶体膜中电子与SO声子耦合强,与LO声子耦合弱的电子-声子相互作用系统的有效质量.得出了束缚极化子的有效质量随膜厚的变化规律.对KCl材料进行数值计算的结果表明,不同支声子与电子相互作用对有效质量的贡献并不相同,而且由于束缚势的存在,使有效质量增大.

关键词：极性晶体膜束缚极化子线性组合算符有效质量膜厚

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于涉入理论的自驾车旅游购物行为实证研究——以少林寺景区为例

引用

经济地理 2009年第10期29卷 1748-1752页

作者：钟志平王秀娟湖南商学院旅游管理学院中国湖南长沙410205 湖南商学院旅游规划与区域发展研究所中国湖南长沙410205 湖南师范大学旅游学院中国湖南长沙410081

以少林寺景区自驾车游客为研究对象,用抽样调查的方法取得基础数据,将涉入理论引入到自驾车游客购物行为的研究中,分析不同涉入程度的自驾车游客的购物行为的差异,为相关生产、销售、服务、管理等部门提供参考。

关键词：涉入自驾车游客购物行为少林寺景区

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

非球面光学设计技术综述

引用

激光杂志 2006年第3期27卷 1-2页

作者：勾志勇王江王楚王磊吴志海伍丽莎王秀芳西南技术物理研究所成都610041 四川大学原子分子物理研究所成都610064

非球面光学在工业、国防和商业等领域的应用中具有十分重要的意义。当用非球面设计光学系统时,非球面方程的选用十分重要,决定着系统最佳优化结果。文章介绍了非球面光学技术的发展过程,分析了常用非球面的应用方程,井对非球面应用方程... 详细信息

非球面光学在工业、国防和商业等领域的应用中具有十分重要的意义。当用非球面设计光学系统时,非球面方程的选用十分重要,决定着系统最佳优化结果。文章介绍了非球面光学技术的发展过程,分析了常用非球面的应用方程,井对非球面应用方程的特点及非球面系数的关系进行了概述,较详细地分析了各类非球面应用方程的适用范围,最后概述了非球面光学在军事、工业等领域的应用以及发展趋势。

关键词：非球面光学非球面透镜非球面系数光学设计

来源：

维普期刊数据库

同方期刊数据库评论