检索结果-南通市图书馆

Extraction Hydrolysates from Larimichthys Polyactis Swim Bladder Using Enzymatic Hydrolysis

在线全文

学校读者我要写书评

暂无评论

Journal of Renewable Materials 2021年第6期9卷 1099-1109页

作者： Ling Pan Yubo tao peng Li College of Material Science and Engineering Northeast Forestry UniversityHarbin150040China State Key Laboratory of Biobased Material and Green Papermaking Qilu University of TechnologyShandong Academy of SciencesJinan250353China

As a kind of biopolymer,hydrolysates of fish swim bladder,safer than those of land mammals,are widely used in food,cosmetics as well as pharmaceutical and biomedical fields for their biocompatibility,biodegradability,and weak *** enhance hydrolysate production,in this paper,the papain and alcalase hydrolysis processes of larimichthys polyactis swim bladder were optimized with orthogonal *** 89.5%hydrolysate yield,the optimal processing conditions for alcalase were solid-liquid ratio of 1:30,enzyme concentration of 0.7%,and extraction time of 6 *** for papain,under the optimal processing conditions:solid-liquid ratio of 1:20,enzyme concentration of 0.5%,and extraction time of 8 h,the hydrolysate yield was 65.1%.To obtain higher hydrolysate yields,the ultrasonic pretreatments were implemented before the optimal enzyme hydrolysis *** ultrasonic waves of 100 W for 50 min,the hydrolysate yields were increased 2.1%(alcalase)and 4.5%(papain),*** Fourier Transform Infrared(FTIR)spectroscopic analysis revealed that the hydrolysates extracted by papain exist in triple-helical *** Ultra-Violet(UV)absorption spectra indicated that the aromatic amino acids in the hydrolysates had strong absorptions in the wavelength range of 240 nm–300 *** results of this research demonstrate that the alcalase hydrolysates have better solubility in water and the solution is more stable under ambient ***,the hydrolysates extracted by papain have a gel property and are insoluble in weak acid at room temperature,which is more suitable for applications in feedstock of biomedical.

关键词： Swim bladder enzymatic hydrolysis ultrasonic-assisted extraction hydrolysates analysis

Toward pluripotency by reprogramming:mechanisms and application

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2013年第11期4卷 820-832页

作者： tao Wang Stephen TWarren peng Jin Department of Human Genetics Emory UniversityAtlantaGA 30322USA Genetics and Molecular Biology Graduate Program Emory UniversityAtlantaGA 30322USA

The somatic epigenome can be reprogrammed to a pluri-potent state by a combination of transcription *** cell fate involves transcription factors coopera-tion,epigenetic reconfi guration,such as DNA methylation and histone modification,posttranscriptional regulation by microRNAs,and so ***,such reprogram-ming is *** suggests that during the early stage of reprogramming,the process is stochastic,but by the late stage,it is *** addition to con-ventional reprogramming methods,dozens of small mol-ecules have been identifi ed that can functionally replace reprogramming factors and signifi cantly improve induced pluripotent stem cell(iPSC)***,iPS cells have been created recently using chemical com-pounds *** are thought to display subtle genetic and epigenetic variability;this variability is not random,but occurs at hotspots across the *** we dis-cuss the progress and current perspectives in the fi *** into the reprogramming process today will pave the way for great advances in regenerative medicine in the future.

关键词： epigenetic reprogramming induced pluripo-tent stem cells embryonic stem cells disease modeling

Production of N-acetylglucosamine with Vibrio alginolyticus FA2,an emerging platform for economical unsterile open fermentation

在线全文

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2023年第3期8卷 546-554页

作者： Yuan peng Ping Xu Fei tao State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic and Developmental Sciencesand School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China

Members of the Vibrionaceae family are predominantly fast-growing and halophilic microorganisms that have captured the attention of researchers owing to their potential applications in rapid *** them,Vibrio alginolyticus FA2 is a particularly noteworthy halophilic bacterium that exhibits superior growth *** has the potential to serve as a biotechnological platform for sustainable and eco-friendly open fermentation with *** evaluate this hypothesis,we integrated the N-acetylglucosamine(GlcNAc)pathway into *** *** nag genes were knocked out to obstruct the utilization of GlcNAc,and then 16 exogenous gna1s co-expressing with EcglmS were introduced to strengthen the flux of GlcNAc pathway,*** further enhance GlcNAc production,we fine-tuned promoter strength of the two genes and inactivated two genes alsS and alsD to prevent the production of ***,unsterile open fermentation was carried out using simulated seawater and a chemically defined medium,resulting in the production of 9.2 g/L GlcNAc in 14 *** is the first report for de-novo synthesizing GlcNAc with a Vibrio strain,facilitated by an unsterile open fermentation process employing seawater as a substitute for fresh *** development establishes a basis for production of diverse valuable chemicals using Vibrio strains and provides insights into biomanufacture.

关键词： Vibrio Open fermentation Metabolic engineering N-acetylglucosamine

PREDICTION OF HYDRODYNAMIC PERFORMANCE OF THE FLAP RUDDER

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Hydrodynamics 2000年第3期12卷 87-92页

作者： Wang, Lei Yang, Jianmin peng, tao Shanghai Jiaotong Univ Shanghai China

In this paper a new method for predicting the hydrodynamic performance of the flap rudder behind a propeller was presented. The hydrodynamics of the rudder was calculated by the panel method and the performance of the propeller was predicted by the simplified propeller theory. The interaction between the rudder and propeller was determined by iterative procedure. The pressure distribution on rudder surface and the hydrodynamic performance of the flap rudder were discussed in the paper.

关键词： Flow of water Hydrodynamics Iterative methods Numerical analysis Ship propellers

Feature extraction method of hyperspectral scattering images for prediction of total viable count in pork meat

在线全文

学校读者我要写书评

暂无评论

International Journal of Agricultural and Biological Engineering 2015年第4期8卷 95-105页

作者： tao Feifei peng Yankun Li Yongyu College of Engineering China Agricultural UniversityBeijing 100083China

This study aimed to investigate the capabilities of hyperspectral scattering imaging in tandem with Gaussian function,Exponential function and Lorentzian function for rapid and nondestructive determination of total viable count(TVC)in pork *** batches of fresh pork meat was purchased from a local market and stored at 10°C for 1-9 *** 60 samples were used,and several samples were taken out randomly for hyperspectral scattering imaging and conventional microbiological tests on each day of the *** functions of Gaussian,Exponential and Lorentzian were employed to model the hyperspectral scattering profiles of pork meat,and good fitting results were obtained by all three functions between 455 nm and 1000 *** Lorentzian function performed best for fitting the hyperspectral scattering profiles of pork meat compared with other *** principal component regression(PCR)and partial least squares regression(PLSR)methods were performed to establish the prediction *** all the developed models,the models developed using parameters CE(scattering width parameter of Exponential function)and CL(scattering width parameter of Lorentzian function)by PLSR method gave superior results for predicting pork meat TVC,with RV and RMSEV of 0.92,0.59 log CFU/g,and 0.91,0.61 log CFU/g,*** addition,based on the improved hyperspectral scattering system,parameter c which represented the scattering widths in all three functions gave more accurate prediction results,regardless of the modeling methods(PCR or PLSR).The obtained results demonstrated that hyperspectral scattering imaging combined with the presented data analysis algorithm can be a powerful tool for evaluating the microbial safety of meat in the future.

关键词： hyperspectral scattering imaging pork meat total viable count Lorentzian function Gaussian function Exponential function

An intra-articular injectable phospholipids-based gel for the treatment of rheumatoid arthritis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Pharmaceutical Sciences 2023年第1期18卷 120-131页

作者： Yuping Yang Shiqin Luo xiong peng Ting Zhao Qin He Mengying Wu Wei Zhang tao Gong Zhirong Zhang Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial TechnologyWest China School of PharmacySichuan UniversityChengdu 610064China Department of Pharmacy West China Hospital Sichuan University Jintang HospitalChengdu 610000China

Rheumatoid arthritis(RA)is a chronic inflammatory and destructive arthropathy with a high deformity *** numerous studies and clinical trials,no curative treatment is available for large weight-bearing ***-articular(IA)injections could deliver high concentrations of drug to the afflicted joint and improve the drug efficacy while reducing systemic ***,free drugs are rapidly cleared from synovial fluid and do not significantly halt the progression of joint ***,a phospholipids-based controlledrelease gel was prepared for sustained IA delivery of celastrol(CEL)and the therapeutic efficiencywas evaluated in a rheumatoid arthritis *** CEL-loaded gel(CEL-gel)contained up to 70%phospholipids yetwas easy to *** injecting into the joint cavity,CEL-gel achieved sol to gel phase transition without special stimuli and gelling *** vitro release and in vivo pharmacokinetic studies evidenced the stable and sustained release action of CEL-gel.A single IA injection of CEL-gel could maintain therapeutic efficiency for about 25 d and showed much better anti-arthritic efficacy compared to repeated injections of free drug solution(CEL-sol).Furthermore,the IA injection of CEL-gel greatly reduced the systemic toxicity of *** good biocompatibility and biodegradability,CEL-gel might be a promising IA drug delivery system.

关键词： Celastrol Rheumatoid arthritis Phospholipids-based controlled release gel Intra-articular drug delivery

Screening of methylation genes in age-related cataract

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2018年第7期11卷 1102-1107页

作者： Li Wang peng Li xiong Guo Department of Medicine Technology Optometry Xi’an Medical University Public Health School Health Science Center Xi’an Jiaotong University Department of Ophthalmology the 451st Hospital of the PLA

AIM： To analyze and screen the methylation status of whole-genome in age-related cataract samples. METHODS： Anterior lens capsule samples were collected from age-related cortical cataract patients over 50 years of age with LOCS III score of nuclear color ≥4 along with control subjects. DNAs were extracted and subjected to methylation microarray for the identification of methylated genes employing the high-throughput sequencing approach. RESULTS： Compared with the control group, 843 sites were found methylated, including 802 hypermethylation sites with 542 corresponding genes, 41 demethylation sites with 29 corresponding sites. COL4 A1, GJA3, SIPA1 L3 were confirmed by mass spectrometry, the results were consistent with high-throughput sequencing. CONCLUSION： DNA methylation microarrays is an efficient way for screening the aberrantly methylated genes. In this study, we are able to screen a few age-related cataract genes such as COL4 A1, GJA3, and SIPA1 L3 for their aberrant methylation patterns in cataract patients however further work is warranted to understand the significance of these findings.

关键词： age-related cataract methylation genes methylation microarrays

Xuefu Zhuyu decoction improves neurological dysfunction by increasing synapsin expression after traumatic brain injury

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2018年第8期13卷 1417-1424页

作者： Lin Zhu tao Tang Rong Fan Jie-Kun Luo Han-Jin Cui Chun-Hu Zhang Wei-Jun peng peng Sun Xin-Gui xiong Yang Wang Laboratory of Ethnopharmacology Institute of Integrated Traditional Chinese and Western Medicine Xiangya Hospital Central South University Changsha Hunan Province China Department of-Integrated Traditional Chinese and Western Medicine Second Xiangya Hospital Central South University Changsha Hunan Province China College of Pharmacy Shandong University of Traditional Chinese Medicine Jinan Shandong Province China

Xuefu Zhuyu decoction has been used for treating traumatic brain injury and improving post-traumatic dysfunction, but its mechanism of action needs further investigation. This study established rat models of traumatic brain injury by controlled cortical impact. Rat models were intragastrically administered 9 and 18 g/kg Xuefu Zhuyu decoction once a day for 14 or 21 days. Changes in neurological function were assessed by modified neurological severity scores and the Morris water maze. Immunohistochemistry, western blot assay, and re- verse-transcription polymerase chain reaction were used to analyze synapsin protein and mRNA expression at the injury site of rats. Our results showed that Xuefu Zhuyu decoction visibly improved neurological function of rats with traumatic brain injury. These changes were accompanied by increased expression of synaptophysin, synapsin I, and postsynaptic density protein-95 protein and mRNA in a dose-de- pendent manner. These findings indicate that Xuefu Zhuyu decoction increases synapsin expression and improves neurological deficits alder traumatic brain injury.

关键词： nerve regeneration traumatic brain injury Xuefu Zhuyu decoction neurological dysfunction synapsin synaptophysin synapsin I posts vnaptic density protein-95 neuroprotection neural regeneration

Human cancergermlineantigen-specific cytotoxicTcell-what canwelearnfrompatient

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2020年第7期17卷 684-692页

作者： Megat Abd Hamid Yanchun peng tao Dong Nufield Department of Medicine Chinese Academy of Medical Science Oxford Institute(COI)University of OxfordOxfordUK MRC Human Immunology Unit MRC Weatherall Institute of Molecular MedicineUniversity of OxfordOxfordUK

In this review,we will highlight the importance of cancer germline antigen-specific cytotoxic CD8^(+) T lymphocytes(CTL)and the factors affecting antitumor CTL *** light of cancer immunotherapy,we will emphasis the need to further understand the features,characteristics,and actions of modulatory receptors of human cancer germline-specific CTLs,in order to determine the optimal conditions for antitumor CTL responses.

关键词： cytotoxic T cell immune check point receptor immune therapy T cell receptor cancer antigen