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Journal of Reproduction and Contraception 2010年第2期21卷 67-77页

作者： Dong-hui HUANG Hu ZHAO Yu MING Cheng-liang xiong Family Planning Research Institute Tongji Medical College Huazhong University of Science & Technology Wuhan 430030 China Department of Human Anatomy Tongji Medical College Huazhong University of Science & Technology Wuhan 430030 China

Objective To study the functions of urokinase receptor （uPAR） during spermatogenesis by adding targeted siRNA cocktails to the co-culture of Sertoli cell with spermatogenic cells. Methods The seminiferous tubule samples from SD rats aged 20-23 d were digested with collagenase for 1.-20 rain, then were cut into smaU fragments. Tubular fragments were digested with collagenase again for 5 -1. min, then gently resuspended in F1./ DMEM supplemented with vitamins and hormones. Cell samples were seeded in plate and cultured at 32 ℃ for 2 weeks. After 48 h, siRNA cocktails at a concentration of 1. nmol/L or 30 nmol/L were introduced into this cocultured Sertoli/spermatogenic cells. After then for 48 h, expression of uPAR mRNA was analyzed by real-time RT-PCR. Results Some spermatogenic cells took place morphologic changes and generated an obvious flagellum. The expression levels of uPAR mRNA silencing with siRNA cocktails at a concentration of 1. nmol/L or 30 nmol/L were significantly lower than those in nontransfected group （P〈0.05）; the inhibition rate was 63.5% or 76.7%, respectively. Conclusion The meiosis and spermiogenesis could take place in this culture system, and the siRNA cocktails can effectively inhibit the gene expression of uPAR in co-cultured Sertoli/spermatogenic cells.

关键词： small interfering RNA RNA interference urokinase receptor (uPAR) spermatogenic cell cell co-cultures

Integrated Survivability Strategies of IP/GMPLS/Optical Multi-layer Network

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Semiconductor Photonics and Technology 2003年第3期9卷 141-147页

作者： ZHAO Ji jun 1.2 , JI Yue feng 1. XU Da xiong 1.(1. Opt.Commun.Center,Beijing University of Posts and telecommunications,Beijing 1.0876,CHN 2. Hebei Institute of Architectural Science and Technology, Handan 056038, CHN) Opt. Coinmun. Center Beijing University of Posts and telecommunications 北京 100876

In last decade,due to that the popularity of the internet, data-central traffic kept growing,some emerging networking requirements have been posed on the todays telecommunication networks,especially in the area of network ***,as a key networking problem,network reliability will be more and more *** integration of different technologies such as ATM,SDH,and WDM in multilayer transport networks raises many questions regarding the coordination of the individual network *** problem is referred as multilayer network *** integrated multilayer network survivability is investingated as well as the representation of an interworking strategy between different single layer survivability schemes in IP via generalized multi-protocol label switching over optical network.

关键词： optical network multilayer survivability GMPLS

晚更新世末至公元前7世纪的黄河流向和黄河三角洲

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海洋学报 2004年第1期26卷 48-61页

作者：薛春汀周永青朱雄华青岛海洋地质研究所山东青岛266071

大约距今9600～8500a黄河在苏北北部入黄海.黄河口与其南侧的长江口相距1.0多千米.两条大河巨大数量的入海泥砂形成了黄河-长江复合三角洲.根据南黄海西部全新世海相沉积物厚度变化、苏北响水县陈家巷QC4孔沉积序列、阜宁以南、泰州以... 详细信息

大约距今9600～8500a黄河在苏北北部入黄海.黄河口与其南侧的长江口相距1.0多千米.两条大河巨大数量的入海泥砂形成了黄河-长江复合三角洲.根据南黄海西部全新世海相沉积物厚度变化、苏北响水县陈家巷QC4孔沉积序列、阜宁以南、泰州以北里下河洼地早全新世沉积序列及苏北沿岸砂脊的物质组成判断,距今8500a左右黄河向北流注入渤海,距今8500～7000a海面上升过程中没有形成黄河水下三角洲,距今7000a黄河三角洲又开始形成.

关键词：黄河流向黄河三角洲晚更新世全新世渤海黄海

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池1.井多鱼头并插深度腐蚀油管打捞技术

天然气工业 2005年第8期25卷 70-72页

作者：何春容范兴亮王权唐洪俊范军熊继有四川石油管理局川东钻探公司四川石油管理局川东开发公司重庆科技学院西南石油学院

池1.井井下油管采气1.年后,井下油管严重腐蚀断落,全井形成长片、碎块、断根,其中部分井段呈现双鱼头、3鱼头叠插等情况,该井通过下各种打捞筒、母锥外捞,下各式捞矛外捞、下金刚石铣筒套铣相结合的方式,分段打捞处理。其间针对落鱼特点... 详细信息

池1.井井下油管采气1.年后,井下油管严重腐蚀断落,全井形成长片、碎块、断根,其中部分井段呈现双鱼头、3鱼头叠插等情况,该井通过下各种打捞筒、母锥外捞,下各式捞矛外捞、下金刚石铣筒套铣相结合的方式,分段打捞处理。其间针对落鱼特点,不断修改工具内径或挡圈,加工了特拉式捞筒、滑块开窗捞筒、自制钢丝捞筒等多种适用性工具,最后成功打捞完多鱼头井段。文章总结了打捞过程及技术要点,可供现场生产作业借鉴。

关键词：四川盆地东油管腐蚀打捞技术

增强相对高速电弧喷涂Fe-Al涂层性能的影响

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上海交通大学学报 2005年第1期39卷 36-40页

作者：徐维普徐滨士张伟吴毅雄上海交通大学材料科学与工程学院上海200030 装甲兵工程学院装备再制造技术国防科技重点实验室北京100072

采用添加增强相的Fe-Al粉芯丝材和高速电弧喷涂技术(HVAS)原位合成了复合涂层.对添加了不同增强相的涂层组织和性能进行了研究,分析了陶瓷增强相的选择对涂层物理和化学性能的影响.结果表明,对高速电弧喷涂Fe-Al/WC涂层和Fe-Al/Cr3C2涂... 详细信息

采用添加增强相的Fe-Al粉芯丝材和高速电弧喷涂技术(HVAS)原位合成了复合涂层.对添加了不同增强相的涂层组织和性能进行了研究,分析了陶瓷增强相的选择对涂层物理和化学性能的影响.结果表明,对高速电弧喷涂Fe-Al/WC涂层和Fe-Al/Cr3C2涂层的性能比较显示,在丝材中添加Cr3C2增强相对比添加WC增强相,可以显著提高铁铝涂层的耐冲蚀以及耐腐蚀性能,明显提高涂层的常温耐磨损性能,并有效提高涂层的结合强度.

关键词：增强相 Fe-Al涂层高速电弧喷涂

Computational manufacturing

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Progress in Natural Science:Materials International 2002年第9期 3-10页

作者： DING Han and xiong Youlun(1. School of Mechanical Engineering, Shanghai Jiaotong University, Shanghai 200030, China 2. School of Mechanical Engineering & Science, Huazhong University of Sci. & Tech., Wuhan 430074, China) School of Mechanical Engineering Shanghai Jiaotong University Shanghai 200030 China School of Mechanical Engineering & Science Huazhong University of Sci. & Tech. Wuhan 430074 China

This paper presents a general framework for computational manufacturing. The methodology of computational manufacturing aims at integrating computational geometry, machining principle, sensor information fusion, optimization, computational intelligence and virtual prototyping to solve problems of the modeling, reasoning, control, planning and scheduling of manufacturing processes and systems. There are three typical problems in computational manufacturing, i.e., scheduling (time-domain), geometric reasoning (space-domain) and decision- making (interaction between time-domain and space-domain). Some theoretical fundamentals of computational manufacturing are also discussed.

关键词： computational manufacturing geometric reasoning computational Intelligence Internet-based control and maintenance virtual prototyping.

Effect of Nano Red Elemental Selenium on GPx Activity of Broiler Chick Kidney Cells in vitro

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Wuhan University Journal of Natural Sciences 2003年第4A期8卷 1161-1166页

作者： Xu Bao-hua 1.2 , Xu Zi-rong 1. , Xia Mei-sheng 1. Hu Cai-hong 1. Deng Yue-song 1. xiong Li 1.1. College of Animal Science, Zhejiang University, Hangzhou 31.029, Zhejiang, China 2. College of Animal Science and Technology, Shandong Agricultural University, Taian 271.1., Shandong, China College of Animal Science Zhejiang University Hangzhou 310029Zhejiang China College of Animal Science and Technology Shandong Agricultural University Taian 271018 Shandong China College of Animal Science Zhejiang University Hangzhou 310029Zhejiang China

A new selenium source, Nano red elemental selenium (Nano-Se) was used to study the effect on the GPx activity of broiler chick kidney cells (BCKC) in vitro, Sodium selenite (Na_ 2 SeO_ 3 ) and seleno-1.methionine (Se-Met) were used as the controls. The results showed that the effects of three kinds of Se forms on the GPx activity of BCKC were accordant(p>0.05) compared with each other at 0.01.0.05 and 0.1. μmol/L Se concentrations treatments. In the range of 0.00-0.1. μmol/L Se concentrations, the GPx activity increased with elevation of Se concentrations in medium. For the three kinds of Se forms, the GPx activity reached the climax at 0.1. μmol/L Se concentration. At 0.20 and 0.30 μmol/L Se concentrations, the influnces of three kinds of Se forms were not accordant with one another. For Nano-Se, the GPx activity at 0.20 and 0.30 μmol/L Se concentrations remained the same as that at 0.1. μmol/L Se concentration treatment. For Se-Met, the GPx activity at 0.20 μmol/L Se concentration treatment remained the same with 0.1. μmol/L treatment; the GPx activity at 0.30 μmol/L Se concentration treatment was declined significantly(p1. or 0.20 μmol/L treatment. For Na_ 2 SeO_ 3 , the GPx activity falled gradually with Se concentration increasing from 0.1. μmol/L to 0.30 μmol/L, and at 0.30 μmol/L Se concentration treatment, the GPx activity was less than the original of BCKC. The results implicated, on the GPx activity of BCKC in vitro, the ranking of width range of the most suitable Se concentration for nutrition curve of the three Se formes is Nano-Se>Se-Met>Na_ 2 SeO_ 3 .

关键词： Nano-Se GPx activity broiler chick kidney cells (BCKC) cell culture

A Dual Yamabe Flow and Related Integral Flows

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Chinese Annals of Mathematics,Series B 2024年第3期45卷 319-348页

作者： Jingang xiong School of Mathematical Sciences Laboratory of Mathematics and Complex SystemsMinistry of EducationBeijing Normal UniversityBeijing 100875China

The author studies a family of nonlinear integral flows that involve Riesz potentials on Riemannian manifolds. In the Hardy-Littlewood-Sobolev (HLS for short)subcritical regime, he presents a precise blow-up profile exhibited by the flows. In the HLS critical regime, by introducing a dual Q curvature he demonstrates the concentrationcompactness phenomenon. If, in addition, the integral kernel matches with the Green’s function of a conformally invariant elliptic operator, this critical flow can be considered as a dual Yamabe flow. Convergence is then established on the unit spheres, which is also valid on certain locally conformally flat manifolds.

关键词： Hardy-Littlewood-Sobolev functional Dual Q curvature Integral flow

Convex-up Style of Deformation within Grabens in Regions of Incomplete Crossing Conjugate Normal Faults: A Numerical Simulation Investigation and Case Study

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Journal of Earth Science 2024年第3期35卷 839-849页

作者： Zheng Li Kai Wang xiong xiong Bing Zhou School of Geophysics and Geomatics China University of GeosciencesWuhan 430074China State Key Laboratory of Geodesy and Earth’s Dynamics Innovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan 430077China Department of Earth Sicences Khalifa UniversityAbu DhabiUnited Arab Emirates

Crossing conjugate normal faults(CCNFs)are extensively developed in many hydrocarbon-producing basins,generally existing in the form of incomplete ***,the effect of the non-conjugate zone of the CCNFs on the conjugate relay zone post late tectonic action has not been previously *** use 3D elastic-plastic modeling to investigate the influence of incomplete(i.e.,partially intersecting)CCNFs on the pattern of deformation of strata in the intersection region.A series of model simulations were performed to examine the effects of horizontal tectonic extension,fault size,and fault depth on the deformation of conjugate relay zones of incomplete *** analyses yielded the following results.(1.The model of incomplete conjugation predicts a convex-up style of deformation in the conjugate graben region superimposed on overall subsidence under applied horizontal tectonic extension.(2)The degree of convex-up deformation of the conjugate graben depends on the influence of the non-conjugate zone on the conjugate relay zone,which varies with the amount of horizontal tectonic extension,fault size,and fault burial depth.(3)Our results indicate that incomplete CCNFs can form convex-up deformation,similar to that in the Nanpu Sag area and provide a sound understanding of hydrocarbon migration and accumulation.

关键词： incomplete conjugate normal fault conjugate graben convex-up deformation hydrocarbons Nanpu Sag tectonics.

Light People:Professor Che Ting Chan,curiosity drives to create the impossibilities

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Light(Science & Applications) 2024年第7期13卷 1229-1233页

作者： Tingting Sun Ying xiong Light Publishing Group Changchun Institute of OpticsFine Mechanics and PhysicsChinese Academy of SciencesNo.3888 Dong Nanhu RoadChangchun 130033China College of Intelligence Science and Technology National University of Defense TechnologyNo.109 Deya RoadChangsha 410073China

Editorial“When something is said to be impossible,there are two points for researchers to initially clarify:whether it really is forbidden by the laws of nature;or whether it is simply that no material that currently exists in nature can do that.”Metamaterials are such magical beings,which have physical properties like invisibility,negative refraction,super-resolution,and perfect absorption that are absent from natural *** has been rated by Science as one of the top ten scientific and technological breakthroughs affecting human beings in the 21.t *** this issue of Light People,we spoke with a“magic”creator,Professor Che Ting Chan,the Associate VicePresident(Research&Development)of the Hong Kong University of Science and Technology(HKUST),Member of the Hong Kong Academy of Sciences and Fellow of the American Physical *** has researched a number of theoretical problems in material physics,investigated the theory behind what they seek to achieve,and modulated light(electromagnetism)and acoustic waves through *** the following,let’s take a closer look at Professor Che Ting Chan’s research life,and appreciate his style and the background of his accomplishment.

关键词： breakthrough closer visibility