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MdDREB2A in apple is involved in the regulation of multiple abiotic stress responses

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Horticultural Plant Journal 2021年第3期7卷 197-208页

作者： xinyu Lian xinyu zhao Qiang zhao Guiluan Wang Yuanyuan Li Yujin Hao State Key Laboratory of Crop Biology Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai’anShandong 271018China College of Horticulture Qingdao Agricultural UniversityQingdaoShandong 266109China

Abiotic stress has a serious effect on plant *** transcription factor DREB2A is a member of the AP2/ERF family,which is widely involved in abiotic stress ***,the function of apple MdDREB2A has not been systematically *** this study,MdDREB2A was isolated from the cultivar‘Royal Gala’.The open reading frame of MdDREB2A was 1197 bp in length and it encoded a protein of 398 amino acidswithmolecularweight of 43.8 *** a transcription factor,MdDREB2Awas located in the ***-PCR analysis showed that MdDREB2A was involved in responses to drought,salt,and ABA *** these stress treatments,the relative electrical conductivity,superoxide anion and malondialdehyde(MDA)in transgenic materials significantly decreased,and the content of proline increased in MdDREB2A transgenic plants,compared to the controls,indicating that MdDREB2A transgenic apple calli and transgenic Arabidopsis exhibited improved resistance to abiotic *** study introduces a candidate gene for the genetic improvement of crop resistance and reveals important function of MdDREB2A in the regulation of abiotic stress response.

关键词： Apple MdDREB2A Function Abiotic stress

Effects of Freestream Turbulence,Reynolds Number and Mach Number on the Boundary Layer in a Low Pressure Turbine

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Journal of Thermal Science 2023年第4期32卷 1393-1406页

作者： DUAN Wenhua QIAO Weiyang CHEN Weijie zhao xinyu Turbomachinery Aerodynamics and Acoustics Lab(TAAL) School of Power and EnergyNorthwestern Polytechnical UniversityXi’an710129China

In order to investigate the aerodynamics of a high speed low pressure turbine works in high Mach number and low Reynold number environment,the effect of freestream turbulence(FST)on the boundary layer development on the high speed low pressure turbine under different Reynolds numbers(Re)is numerically *** eddy simulation is adopted here with a subgrid scale model of Wall Adapting Local Eddy viscosity(WALE).Cases with Re ranging from 100000 to 400000 under an exit Mach number(Ma)of 0.87 have been considered at low and high FST levels.A low Ma case(0.17)under very low Re has also been studied under both low and high *** is found that higher Re or FST level leads to earlier *** has a greater effect than FST on the development of boundary *** effect of FST on the boundary layer depends on the *** boundary layer development shows totally different behaviors under different Ma.A separation bubble could be formed under low Ma while no attachment could be detected under high *** FST has a stronger effect on the separated boundary layer under low Ma,which could eliminate the separation in the present *** all the cases under low FST,the Kelvin-Helmholtz instability is the dominate mechanism in the transition *** the low Ma case with high FST,the streamwise streaks play a dominant role in the transition *** the high Ma cases with high FST,both the streamwise streaks and Kelvin-Helmholtz instability work in the transition *** streamwise streaks play a more important role when the Re increased.

关键词： freestream turbulence Reynolds number Mach number low pressure turbine boundary layer

Epigenetic regulation of neuronal dendrite and dendritic spine development

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Frontiers in Biology 2010年第4期5卷 304-323页

作者： Richard D.SMRT xinyu zhao Department of Neuroscience University of New Mexico School of MedicineAlbuquerqueNew Mexico 87131USA

Dendrites and the dendritic spines of neurons play key roles in the connectivity of the brain and have been recognized as the locus of long-term synaptic plasticity,which is correlated with learning and *** development of dendrites and spines in the mammalian central nervous system is a complex process that requires specific molecular events over a period of *** has been shown that specific molecules are needed not only at the spine’s point of contact,but also at a distance,providing signals that initiate a cascade of events leading to synapse *** specific molecules that act to signal neuronal differentiation,dendritic morphology,and synaptogenesis are tightly regulated by genetic and epigenetic *** has been shown that the dendritic spine structure and distribution are altered in many diseases,including many forms of mental retardation(MR),and can also be potentiated by neuronal activities and an enriched *** dendritic spine pathologies are found in many types of MR,it has been proposed that an inability to form normal spines leads to the cognitive and motor deficits that are characteristic of *** mechanisms,including DNA methylation,chromatin remodeling,and the noncoding RNA-mediated process,have profound regulatory roles in mammalian gene *** study of epigenetics focuses on cellular effects that result in a heritable pattern of gene expression without changes to genomic *** extensive efforts to understand the molecular regulation of dendrite and spine development,epigenetic mechanisms have only recently been *** this review,we will focus on epigenetic mechanisms that regulate the development and maturation of dendrites and *** will discuss how epigenetic alterations could result in spine abnormalities that lead to MR,such as is seen in fragile X and Rett *** will also discuss both general methodology and recent technological advances in the study of neuronal den

关键词： epigenetics neurodevelopment dendritic spine synapse microRNA methyl-CpG binding protein 2(MeCP2) mental retardation

Revolutionizing Tight Reservoir Production: A Novel Dual-Medium Unsteady Seepage Model for Optimizing Volumetrically Fractured Horizontal Wells

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Energy Engineering 2023年第12期120卷 2933-2949页

作者： xinyu zhao Mofeng Li Kai Yan Li Yin School of Geosciences Yangtze UniversityWuhan430100China Downhole Service Company Petro China Qinghai Oilfield CompanyHaixi817000China Drilling and Production Technology Research Institute Petro China Qinghai Oilfield CompanyHaixi817000China

This study presents an avant-garde approach for predicting and optimizing production in tight reservoirs,employing a dual-medium unsteady seepage model specifically fashioned for volumetrically fractured horizontal *** models often fail to fully capture the complex dynamics associated with these unconventional *** a significant departure from these models,our approach incorporates an initiation pressure gradient and a discrete fracture seepage network,providing a more realistic representation of the seepage *** model also integrates an enhanced fluid-solid interaction,which allows for a more comprehensive understanding of the fluid-structure interactions in the *** is achieved through the incorporation of improved permeability and stress coupling,leading to more precise predictions of reservoir *** numerical solutions derived from the model are obtained through the sophisticated finite element method,ensuring high accuracy and computational *** ensure the model’s reliability and accuracy,the outcomes were tested against a real-world case,with results demonstrating strong alignment.A key revelation from the study is the significant difference between uncoupled and fully coupled volumetrically fractured horizontal wells,challenging conventional wisdom in the ***,the study delves into the effects of stress,fracture length,and fracture number on reservoir production,contributing valuable insights for the design and optimization of tight *** findings from this study have the potential to revolutionize the field of tight reservoir prediction and management,offering significant advancements in petroleum *** proposed approach brings forth a more nuanced understanding of tight reservoir systems and opens up new avenues for optimizing reservoir management and production.

关键词： Tight reservoirs production prediction model stress effects fractured horizontal well

Room-temperature synthesis of full-component APbX_(3)perovskite nanocrystal inks for optoelectronic applications

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Journal of Energy Chemistry 2024年第5期92卷 87-94页

作者： xinyu zhao Du Li Xuliang Zhang Hehe Huang Chenyu zhao Wanli Ma Jianyu Yuan Institute of Functional Nano&Soft Materials(FUNSOM) Soochow UniversitySuzhou 215123JiangsuChina Jiangsu Key Laboratory for Carbon-Based Functional Materials&Devices Soochow UniversitySuzhou 215123JiangsuChina Jiangsu Key Laboratory of Advanced Negative Carbon Technologies Soochow UniversitySuzhou 215123JiangsuChin

Lead halide perovskite nanocrystals(PNCs)have received great research interests due to their excellent optoelectronic ***,high temperature,inert gas protection and insulating long-chain ligands are used during the conventional hot-injection synthesis of PNCs,which limits their practical *** this work,we first develop a simple and scalable polar-solvent-free method for the preparation of full-component APbX_(3)(A=Cs,methylammonium(MA),formamidinium(FA),X=Cl,Br,I)PNCs under ambient *** an exothermic reaction between butylamine(BA)and propionic acid(PA)short ligands,the PbX_(2) precursors could be well dissolved without use of any polar ***,the relatively lower growth rate of PNCs in our room-temperature reaction enables us to modulate the synthetic procedure to enhance the scalability(40-fold)and achieve large-scale *** resultant short ligands passivated PNC inks are compatible with varying solution depositing technique like spray coating for large-area ***,we showcase that adopting the as-prepared MAPbI_(3) PNC inks,a self-powered photodetector is fabricated and shows a high *** results demonstrate that our ambient-condition synthetic approach can accelerate the preparation of tunable and ready-to-use PNCs towards commercial optoelectronic applications.

关键词： Room-temperaturesynthesis Perovskite nanocrystals Short-chainligands Large-scale Photodetector

Assembly of Nanowires into Macroscopic One‑Dimensional Fibers in Liquid State

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Advanced Fiber Materials 2023年第3期5卷 928-954页

作者： Wentao Cao xinyu zhao Bingqiang Lu Daxiang Cui Feng Chen Center for Orthopaedic Science and Translational Medicine Department of OrthopaedicsShanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai 200072People’s Republic of China National Engineering Research Center for Nanotechnology Shanghai 200241People’s Republic of China Institute of Micro‑Nano Science and Technology School of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China

Natural structural materials,such as spider silk,wood,and bone,are universally acknowledged as the gold standard for the ideal combinations of strength and *** exceptional integrated performance of these biological materials can be ascribed to their multiscale hierarchical architectures and *** the hierarchical assembly feature of natural materials,artificial fibers,which are generated through the one-dimensional(1D)assembly of nanowires,have been widely reported with remarkable flexibility and ***,the distinguishing feature of nanowires’1D assembly can bridge the unique properties of nanowires with their potential functional *** tutorial review summarizes the recent developments in the assembly of nanowires into macroscopic 1D fibers in the liquid *** begin by introducing the general strategies and mechanisms for assembling nanowires in one direction and then,illustrate their potential applications in energy storage,sensors,biomedical engineering,***,a brief summary and some personal perspectives on the future research directions of nanowires’1D assembly are also proposed.

关键词： One-dimensional assembly Nanowires Fibers Liquid state

Investigation of the decline of Ulva prolifera in the Subei Shoal and Qingdao based on physiological changes

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Journal of Oceanology and Limnology 2021年第3期39卷 918-927页

作者： Ya HAO Chen GUAN xinyu zhao Tongfei QU Xuexi TANG Ying WANG College of Marine Life Sciences Ocean University of ChinaQingdao 266100China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China

It has been a scientific consensus that Ulva prolifera green tide that break out annually between May and August in the recent decade off eastern coast of Yellow *** investigate the ecological mechanism of the decline of *** green tide,we compared the physiological characteristics of *** samples collected in three stages of the green tide in Subei Shoal north Jiangsu,the initiation point,on May 10(early,the initiation),July 10(middle,the outbreak),and in Taipingjiao,Qingdao on August 10(late,the decline).Compared to the early samples,the middle,and late samples showed a lower chlorophyll content and photosynthetic e fficiency at a higher lipid peroxidation *** middle and later samples had fewer chloroplasts in the cells of *** than those in the early *** addition,the cells of *** sampled in the late stage showed ruptured and deformed cell *** in physiological function were reflected in the growth *** the middle and late *** samples presented negative growth *** analysis results show that the rising temperature was mainly responsible for the local decline of *** green *** high light-intensity that could induce the photoinhibition was also an important factor for the ***,*** population that remained in the Subei Shoal and those that northward drifted to Qingdao experienced the same decline processes:rising temperature and continuous high light conditions in the late phase of green tide reduced the photosynthetic capacity and destroyed the antioxidant system of the thallus,which lead to decrease of *** only difference is that the temperature in the Subei Shoal rose earlier than that in Qingdao;thus,*** remaining in the Subei Shoal went disappeared first.

关键词： Ulva prolifera green tide decline process photoinhibition lipid peroxidation

Gaussian process regression for prediction and confidence analysis of fruit traits by near-infrared spectroscopy

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Food Quality and Safety 2023年第1期7卷 132-137页

作者： Xiaojing Chen Jianxia Xue Xiao Chen xinyu zhao Shujat Ali Guangzao Huang College of Electrical and Electronic Engineering Wenzhou UniversityWenzhouChina Wenzhou Institute of Industry&Science WenzhouChina

Detection of fruit traits by using near-infrared(NIR)spectroscopy may encounter out-of-distribution samples that exceed the generalization ability of a constructed calibration ***,confidence analysis for a given prediction is required,but this cannot be done using common calibration models of NIR *** address this issue,this paper studied the Gaussian process regression(GPR)for fruit traits detection using NIR *** mean and variance of the GPR were used as the predicted value and confidence,*** show this,a real NIR data set related to dry matter content measurements in mango was *** to partial least squares regression(PLSR),GPR showed approximately 14%lower root mean squared error(RMSE)for the in-distribution test *** with no confidence analysis,using the variance of GPR to remove abnormal samples made GPR and PLSR showed approximately 58%and 10%lower RMSE on the mixed distribution test set,respectively(when the type 1 error rate was set to 0.1).Compared with traditional one-class classification methods,the variance of the GPR can be used to effectively eliminate poorly predicted samples.

关键词： Near-infrared spectroscopy fruit traits calibration model confidence analysis Gaussian process regression

Photosynthetic response of floating Ulva prolifera to diurnal changes of in-situ environments on the sea surface

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Journal of Oceanology and Limnology 2019年第2期37卷 589-599页

作者： zhao xinyu TANG Xuexi HU Shunxin ZHONG Yi QU Tongfei WANG Ying College of Marine Life Sciences Ocean University of China Qingdao 266003 China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China

During yellow sea green tide outbreak, the thalli of Ulva prolifera drifted more than 350 km on the sea surface from off shore of Jiangsu Province to Qingdao city, where they were exposed to complex changes of local environments. The purpose of this study is to investigate the response of the thalli to diurnal changes of environments on the sea surface, and a sea surface environment simulator (SSES) was designed to simulate the natural environment of floating U . prolifera mat. A control experiment is designed in the laboratory, which was processed under suitable conditions (20°C for temperature, 72 μmol photons/(m 2 ·s) for light intensity and 30 for salinity), and an in-situ research was conducted to study the photosynthetic responses of floating U . prolifera to diurnal changes of environments. The results show that the photosynthetic efficiency of the thalli decreased gradually with time, and decreased rapidly after 14:00 local time (LT). After exposed to the environment on the sea surface for 6 h, the photosynthetic activity of the thalli decreased significantly. Furthermore, physiological-level and molecular-level experiments revealed that non-photochemical quenching (NPQ), cyclic electron flow (CEF) and energy redistribution between PSI and PSII all played an important role in the strong photosynthetic plasticity of U . prolifera . NPQ is the most important photoprotective responses to environmental changes before 12:00 LT. The results also confirmed that the CEF and energy redistribution between PSI and PSII are the main synergistic eff ects for the thalli to adapt to the environmental changes when the process NPQ cannot work. And the result can further reveal the reason why U . prolifera can adapt to the living condition of long distance drift on the sea surface. The findings of this research could provide a theoretical basis for explaining outbreaks of the green tide and instructing the management of the problem.

关键词： photosynthetic system floating Ulva prolifera diurnal change photosynthetic plasticity