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Lipid concentration and composition in xylem sap of woody angiosperms from a tropical savanna and a seasonal rainforest

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Plant Diversity 2024年第1期46卷 126-133页

作者： Ling-Bo Huang xinyi Guan Amy Ny Aina Aritsara Jun-Jie Zhu Steven Jansen Kun-Fang cao State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangxi Key Laboratory of Forest Ecology and ConservationGuangxi UniversityDaxuedong Road 100NanningGuangxi 530004China Institute of Botany Ulm UniversityAlbert-Einstein-Allee 11Ulm89081Germany

Lipids may play an important role in preventing gas embolisms by coating nanobubbles in xylem sap.Few studies on xylem sap lipids have been reported for temperate plants,and it remain unclear whether sap lipids have adaptational significance in tropical plants.In this study,we quantify the lipid composition of xylem sap for angiosperm species from a tropical savanna(seven species)and a seasonal rainforest(five species)using mass spectrometry.We found that all twelve species studied contained lipids in their xylem sap,including galactolipids,phospholipids and triacylglycerol,with a total lipid concentration ranging from 0.09 to 0.26 nmol/L.There was no difference in lipid concentration or composition between plants from the two sites,and the lipid concentration was negatively related to species’open vessel volume.Furthermore,savanna species showed little variation in lipid composition between the dry and the rainy season.These results support the hypothesis that xylem sap lipids are derived from the cytoplasm of individual conduit cells,remain trapped inside individual conduits,and undergo few changes in composition over consecutive seasons.A xylem sap lipidomic data set,which includes 12 tropical tree species from this study and 11 temperate tree species from literature,revealed no phylogenetic signals in lipid composition for these species.This study fills a knowledge gap in the lipid content of xylem sap in tropical trees and provides additional support for their common distribution in xylem sap of woody angiosperms.It appears that xylem sap lipids have no adaptive significance.

关键词： Xylem sap Lipids Tropical savanna Tropical rainforest Vessels

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The RNA m^(6)A demethylase ALKBH5 drives emergency granulopoiesis and neutrophil mobilization by upregulating G-CSFR expression

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Cellular & Molecular Immunology 2024年第1期21卷 6-18页

作者： Yang Liu Renjie Song Zhike Lu Lu Zhao xinyi Zhan Yini Li Xuetao cao Department of Immunology Center for ImmunotherapyInstitute of Basic Medical SciencesPeking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Suzhou Institute of Systems Medicine Chinese Academy of Medical SciencesSuzhouChina Frontier Research Center for Cell Response Institute of ImmunologyCollege of Life SciencesNankai UniversityTianjinChina School of Life Sciences Westlake UniversityHangzhouChina

Emergency granulopoiesis and neutrophil mobilization that can be triggered by granulocyte colony-stimulating factor(G-CSF)through its receptor G-CSFR are essential for antibacterial innate defense.However,the epigenetic modifiers crucial for intrinsically regulating G-CSFR expression and the antibacterial response of neutrophils remain largely unclear.N6-methyladenosine(m^(6)A)RNA modification and the related demethylase alkB homolog 5(ALKBH5)are key epigenetic regulators of immunity and inflammation,but their roles in neutrophil production and mobilization are still unknown.We used cecal ligation and puncture(CLP)-induced polymicrobial sepsis to model systemic bacterial infection,and we report that ALKBH5 is required for emergency granulopoiesis and neutrophil mobilization.ALKBH5 depletion significantly impaired the production of immature neutrophils in the bone marrow of septic mice.In addition,Alkbh5-deficient septic mice exhibited higher retention of mature neutrophils in the bone marrow and defective neutrophil release into the circulation,which led to fewer neutrophils at the infection site than in their wild-type littermates.During bacterial infection,ALKBH5 imprinted production-and mobilization-promoting transcriptome signatures in both mouse and human neutrophils.Mechanistically,ALKBH5 erased m^(6)A methylation on the CSF3R mRNA to increase the mRNA stability and protein expression of G-CSFR,consequently upregulating cell surface G-CSFR expression and downstream STAT3 signaling in neutrophils.The RIP-qPCR results confirmed the direct binding of ALKBH5 to the CSF3R mRNA,and the binding strength declined upon bacterial infection,accounting for the decrease in G-CSFR expression on bacteria-infected neutrophils.Considering these results collectively,we define a new role of ALKBH5 in intrinsically driving neutrophil production and mobilization through m^(6)A demethylation-dependent posttranscriptional regulation,indicating that m^(6)A RNA modification in neutrophils is a potential target for treating bacteria

关键词： Emergency granulopoiesis Neutrophil mobilization ALKBH5 m^(6)A RNA modification G-CSF receptor

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Spatiotemporal variation of land surface temperature and its driving factors in Xinjiang,China

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Journal of Arid Land 2024年第3期16卷 373-395页

作者： ZHANG Mingyu cao Yu ZHANG Zhengyong ZHANG Xueying LIU Lin CHEN Hongjin GAO Yu YU Fengchen LIU xinyi College of Sciences Shihezi UniversityShihezi 832000China Key Laboratory of Oasis Town and Mountain-Basin System Ecology Xinjiang Production and Construction CorpsShihezi 832000China Yantai Institute of Coastal Zone Research Chinese Academy of SciencesYantai 264003China

Land surface temperature(LST) directly affects the energy balance of terrestrial surface systems and impacts regional resources, ecosystem evolution, and ecosystem structures. Xinjiang Uygur Autonomous Region is located at the arid Northwest China and is extremely sensitive to climate change. There is an urgent need to understand the distribution patterns of LST in this area and quantitatively measure the nature and intensity of the impacts of the major driving factors from a spatial perspective, as well as elucidate the formation mechanisms. In this study, we used the MOD11C3 LST product developed on the basis of Moderate Resolution Imaging Spectroradiometer(MODIS) to conduct regression analysis and determine the spatiotemporal variation and differentiation pattern of LST in Xinjiang from 2000 to 2020. We analyzed the driving mechanisms of spatial heterogeneity of LST in Xinjiang and the six geomorphic zones(the Altay Mountains, Junggar Basin, Tianshan Mountains, Tarim Basin, Turpan-Hami(Tuha) Basin, and Pakakuna Mountain Group) using geographical detector(Geodetector) and geographically weighted regression(GWR) models. The warming rate of LST in Xinjiang during the study period was 0.24℃/10a, and the spatial distribution pattern of LST had obvious topographic imprints, with 87.20% of the warming zone located in the Gobi desert and areas with frequent human activities, and the cooling zone mainly located in the mountainous areas. The seasonal LST in Xinjiang was at a cooling rate of 0.09℃/10a in autumn, and showed a warming trend in other seasons. Digital elevation model(DEM), latitude, wind speed, precipitation, normalized difference vegetation index(NDVI), and sunshine duration in the single-factor and interactive detections were the key factors driving the LST changes. The direction and intensity of each major driving factor on the spatial variations of LST in the study area were heterogeneous. The negative feedback effect of DEM on the spatial differentiation of LST was the strongest. Lower latitudes, lower

关键词： land surface temperature MOD11C3 climate change geographical detector(Geodetector) geographically weighted regression(GWR) source-sink effect Xinjiang

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Nascent Proteome and Glycoproteome Reveal the Inhibition Role of ALG1 in Hepatocellular Carcinoma Cell Migration

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Phenomics 2022年第4期2卷 230-241页

作者： xinyi cao Yuyin Shao Peiyi Meng Zhao cao Guoquan Yan Jun Yao Xinwen Zhou Chao Liu Lei Zhang Hong Shu Haojie Lu Institutes of Biomedical Sciences and Shanghai Cancer Center Fudan UniversityShanghai 200032China Department of Chemistry and NHC Key Laboratory of Glycoconjugates Research Fudan UniversityShanghai 200433China Department of Clinical Laboratory First Affiliated Hospital of Guangxi Medical UniversityNanning 530021China Beijing Advanced Innovation Center for Precision Medicine Beihang UniversityBeijing 100083China Department of Clinical Laboratory Guangxi Medical University Cancer HospitalNanning 530021China

Asparagine-linked glycosylation protein 1 homolog(ALG1)participates in the initial stage of protein N-glycosylation and N-glycosylation has been implicated in the process of hepatocellular carcinoma(HCC)progression.However,whether ALG1 plays a role in human HCC remains unknown.In this study,the expression profile of ALG1 in tumorous and corresponding adjacent non-tumor tissues was analyzed.The relationship of ALG1 expression with clinical features and prognosis of HCC patients was also evaluated using immuno-histochemical method.Here we found ALG1 decreased in HCC tissues compared with adjacent normal liver tissues,which predicted an unfavorable prognosis.Combined with RNA interference,nascent proteome and glycoproteome were determined systematically in Huh7 cell line.Bioinformatics analysis indicated that the differentially expressed proteins participating in the response of ALG1 knockdown were most significantly associated with cell-cell adhesion.Functional studies confirmed that knockdown of ALG1 reduced cell adhesion capacity,and promoted cell migration.Furthermore,down-regulation of H8N2(on N-glycosite N651)and H5N4S2F1(on N-glycosite N692)from N-cadherin was identified as a feature of ALG1 knockdown.Our findings revealed that ALG1 controlled the expression of glycosylated N-cadherin and played a role in HCC migration,with implications for prognosis.

关键词： Nascent proteome ALG1 Glycosylation N-cadherin Hepatocellular carcinoma

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Water and fluorinated alcohol mediated/promoted tandem insertion/aerobic oxidation/bisindolylation under metal-free conditions:Easy access to bis(indolyl)methanes

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Chinese Chemical Letters 2021年第5期32卷 1696-1700页

作者： Yong Wang xinyi cao Jingfei Ji Xiuling Cui Chao Pi Leyao Zhao Yangjie Wu Henan Key Laboratory of Chemical Biology and Organic Chemistry Key Laboratory of Applied Chemistry of Henan UniversitiesGreen Catalysis Centerand College of ChemistryZhengzhou UniversityZhengzhou 450052China International College Zhengzhou UniversityZhengzhou 450052China

A green tandem reaction,including insertion/aerobic oxidation/bisindolylation,starting from indoles and diazo compounds has been developed.The combination of water and fluorinated alcohol plays dual roles as solvent and promoter in this chemical transformation.Molecular oxygen in the air acts as an oxidant.3,3’-Bis(indolyl)methanes with quaternary carbon were produced under metal-free conditions.No any catalyst and additive were required.N2 and water were released as sole by-products.Absence of water and fluorinated alcohol resulted in Wolff rearrangement product.

关键词： Bis(indolyl)mcthanes Diazo Fluorinated alcohol Indole Water

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从苏格拉底到ChatGPT:教学对话空间的纵深拓展——访剑桥大学鲁珀特·韦格里夫教授

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全球教育展望 2024年第4期53卷 53-63页

作者：于青青曹鑫怡史圣朋北京大学教师教学发展中心北京100871 剑桥大学教育系剑桥CB28PQ 剑桥大学未来教育研究中心剑桥CB12EW

对话教育理论认为教学活动的核心是师生之间的交互过程,即对话的活动。与传统灌输式教学法相异,它强调在对话中异质观点与知识的相互冲撞与融合,从而产生新知识,并重塑对话主体的认知结构与思维模式。鲁珀特·韦格里夫教授是对话教育领... 详细信息

对话教育理论认为教学活动的核心是师生之间的交互过程,即对话的活动。与传统灌输式教学法相异,它强调在对话中异质观点与知识的相互冲撞与融合,从而产生新知识,并重塑对话主体的认知结构与思维模式。鲁珀特·韦格里夫教授是对话教育领域的著名学者,提出了“对话鸿沟”的概念,并主张“跨越对话鸿沟的教学”。他认为对话教育要通过开辟和拓展对话空间来实现,并进一步论证在数字时代背景下,互联网、元宇宙、人工智能等技术的发展能够为对话空间的拓展提供新的维度。特别是在ChatGPT等人工智能技术被广泛应用后,我们不得不重新思考苏格拉底的对话教学理念,以及“对话”在促进学生思维发展、实现知识共建、生成群体智慧等方面的潜力。

关键词：对话教育对话空间 ChatGPT 数字化未来素养

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植物芥子油苷的生物学功能研究进展

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分子植物育种 2024年第7期22卷 2421-2427页

作者：于心怡曹育诚朱乾坤西南交通大学生命科学与工程学院成都610031

芥子油苷(glucosinolates, GSLs),是一类具有营养功能和生物活性的植物次生代谢物。目前对芥子油苷的综述主要集中在芥子油苷的生物合成与基因调控等方面,少有对芥子油苷生物学功能的全面介绍。本研究总结了芥子油苷的生物学功能,讨论... 详细信息

芥子油苷(glucosinolates, GSLs),是一类具有营养功能和生物活性的植物次生代谢物。目前对芥子油苷的综述主要集中在芥子油苷的生物合成与基因调控等方面,少有对芥子油苷生物学功能的全面介绍。本研究总结了芥子油苷的生物学功能,讨论了芥子油苷的生物合成、其在植物应对生物胁迫和非生物胁迫中发挥的作用以及在抗癌作用和天然农药开发中的应用。对芥子油苷与细胞信号传导之间的关系、芥子油苷应对非生物胁迫和植物的防御功能之间的关系等研究方向进行了讨论与展望。以期为芥子油苷及其衍生物的应用开发和相关作物的育种改良等提供理论参考。

关键词：芥子油苷生物胁迫非生物胁迫应用

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J亚群禽白血病病毒血液核酸筛查技术的建立

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畜牧兽医学报 2024年第3期55卷 1115-1126页

作者：董欣怡李锦群陈钦玺廖明曹伟胜华南农业大学兽医学院人兽共患病防控制剂国家地方联合工程实验室农业部兽用疫苗创制重点实验室广东省动物源性人兽共患病预防与控制重点实验室广州510642

为缩短J亚群禽白血病病毒(subgroup J avian leukosis virus, ALV-J)检测周期,进一步加快禽白血病净化进程,本研究结合SYBR GreenⅠqPCR和混样检测,建立了一种适用于低ALV-J流行率场景下的快速筛检ALV-J的血液核酸筛查技术(ALV-J blood ... 详细信息

为缩短J亚群禽白血病病毒(subgroup J avian leukosis virus, ALV-J)检测周期,进一步加快禽白血病净化进程,本研究结合SYBR GreenⅠqPCR和混样检测,建立了一种适用于低ALV-J流行率场景下的快速筛检ALV-J的血液核酸筛查技术(ALV-J blood quantitative polymerase chain reaction, ALV-J-B-qPCR)。根据GenBank中ALV-J pol及env基因序列,设计ALV-J的特异性引物,并优化反应条件,建立了ALV-J SYBR GreenⅠqPCR检测方法。以ALV-J病毒分离为阳性的鸡抗凝血为实验材料,分别制备抗凝血DNA、血细胞DNA、外周血淋巴细胞DNA、外周血淋巴细胞cDNA和血浆cDNA 5类血液检测模板进行ALV-J的PCR检测,筛选最佳检测模板;进一步比较蒸馏水破裂红细胞法提取混合血液DNA的混样方法,血液混样总体积为200μL的混样方法,血液混样总体积为10μL的混样方法共3种混样方法的检测准确性,筛选最佳混样方法。综合上述qPCR方法、检测模板与混样方法,成功建立了ALV-J-B-qPCR。运用ALV-J-B-qPCR分别进行预期流行率为1%~2%、4%~5%场景下的模拟筛查试验,并与病毒分离法比较。qPCR方法的特异性、灵敏性和重复性试验显示,该方法仅特异性扩增ALV-J,对标准质粒的最低检测限度为1×10^(2)copies·μL^(-1),批内与批间变异系数均<1%。对90份临床送检血液样品的检测结果显示,该qPCR方法对ALV-J的检出率(15.6%)高于p27抗原ELISA和普通PCR的检出率(12.2%)。检测模板筛选试验中,抗凝血DNA最符合检测准确度高、操作复杂度和成本低的要求,为最佳检测模板。混样方法摸索试验中,混样总体积为10μL(混样规模<12份)的混样方法检测准确性最高,为最佳混样方法。在样本数为400份,预期流行率为4%~5%场景的模拟筛查中,ALV-J-B-qPCR检出率为6.25%,比病毒分离法高2.00%;在样本数为400份,预期流行率为1%~2%场景的模拟筛查中,ALV-J-B-qPCR检出率为2.25%,比病毒分离法高0.75%。本研究建立的ALV-J-B-qPCR技术具有灵敏性高、检测快速和节约成本的优势,为种禽场加快ALV净化进程提供了新的思路和方法。

关键词： J亚群禽白血病病毒实时荧光定量PCR 混样检测筛查技术

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非一致性条件下松澧地区洪水遭遇规律分析

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水利水电科技进展 2024年第4期44卷 14-22页

作者：隆院男黄春福莫军成黄草宋昕熠闫世雄曹劲松长沙理工大学水利与环境工程学院湖南长沙410114 洞庭湖水环境治理与生态修复湖南省重点实验室湖南长沙410114 湖南省水利水电勘测设计规划研究总院有限公司湖南长沙410007

为研究澧水与松滋河洪水遭遇规律,根据澧水石门站和松滋河新江口站、沙道观站1959—2020年实测日均流量资料,采用非一致性GAMLSS模型优选了年最大洪峰和连续7 d年最大洪量的边缘分布,构建了基于Copula函数的二维联合分布模型,进而得出... 详细信息

为研究澧水与松滋河洪水遭遇规律,根据澧水石门站和松滋河新江口站、沙道观站1959—2020年实测日均流量资料,采用非一致性GAMLSS模型优选了年最大洪峰和连续7 d年最大洪量的边缘分布,构建了基于Copula函数的二维联合分布模型,进而得出不同量级组合下的洪水遭遇概率。结果表明:松澧地区的洪水序列发生了显著变异,基于时变对数正态、伽马、威布尔分布以及Copula联合分布能够较好地反映区域实际洪水特征;松澧地区典型年洪水遭遇以松滋河洪水过程遭遇澧水洪水为主;澧水与松滋河的年最大洪峰或连续7 d年最大洪量的同现概率随着洪水量级的增大而减小,且澧水与松滋河东支的洪水遭遇概率更高;当澧水发生千年一遇大洪水时,与松滋河中小洪水的遭遇概率更高。

关键词：澧水松滋河 Copula函数洪水遭遇联合分布

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中国高水平乒乓球男运动员“前四板”技战术制胜因素研究——基于多因素二元logistic回归

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安徽体育科技 2024年第3期45卷 42-49页

作者：周昕怡曹犇李蕴炜西安航空学院体育部陕西西安710077 陕西师范大学体育学院陕西西安710119

目的:探究我国优秀乒乓球男运动员前4板技战术制胜因素。方法:观察26场重大比赛,依据发抢段、接抢段采集数据。技战术指标为自变量,是否得分为因变量,建立多因素二元logistic回归模型。结果:发抢段中,发半出台效果较差(p<0.001),第3板... 详细信息

目的:探究我国优秀乒乓球男运动员前4板技战术制胜因素。方法:观察26场重大比赛,依据发抢段、接抢段采集数据。技战术指标为自变量,是否得分为因变量,建立多因素二元logistic回归模型。结果:发抢段中,发半出台效果较差(p<0.001),第3板使用冲或拍、变正手效果较好。接抢段中,接发球使用搓、变中间效果较差(p<0.01;p<0.05),第4板进攻类技术和变正手效果较好。结论:发球落点与其余3板的技术和是否变线是技战术制胜的关键因素。

关键词：高水平男运动员乒乓球前四板技战术二元logistic回归

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