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Peri-centrosomal localization of small interfering RNAs in C. elegans

Science China Life Sciences 2025年

作者： Qile Jin Xuezhu Feng Minjie Hong Ke Wang Xiangyang Chen Jiewei Cheng Yan Kuang Xiaoyue Si Mingjing Xu xinya huang Shouhong Guang Chengming Zhu Department of Obstetrics and Gynecology The First Affiliated Hospital of USTC The USTC RNA Institute Hefei National Research Center for Physical Sciences at the Microscale School of Life Sciences Division of Life Sciences and Medicine Biomedical Sciences and Health Laboratory of Anhui Province University of Science and Technology of China School of Basic Medicine Anhui Medical University

The centrosome is the microtubule-organizing center and a crucial part of cell division. Centrosomal RNAs(cnRNAs) have been reported to enable precise spatiotemporal control of gene expression during cell division in many species. Whether and how cnRNAs exist in C. elegans are unclear. Here, using the nuclear RNAi Argonaute protein NRDE-3 as a reporter, we observed potential peri-centrosome localized small interfering(si)RNAs in C. elegans. NRDE-3 was previously shown to associate with pre-mRNAs and pre-rRNAs via a process involving the presence of complementary siRNAs. We generated a GFP-NRDE-3 knock-in transgene through CRISPR/Cas9 technology and observed that NRDE-3 formed peri-centrosomal foci neighboring the tubulin protein TBB-2, other centriole proteins and pericentriolar material(PCM)components in C. elegans embryos. The peri-centrosomal accumulation of NRDE-3 depends on RNA-dependent RNA polymerase(RdRP)-synthesized 22G siRNAs and the PAZ domain of NRDE-3, which is essential for siRNA binding. Mutation of eri-1, ergo-1, or drh-3 significantly increased the percentage of pericentrosome-enriched NRDE-3. At the metaphase of the cell cycle, NRDE-3 was enriched in both the peri-centrosomal region and the spindle. Moreover, the integrity of centriole proteins and pericentriolar material(PCM) components is also required for the peri-centrosomal accumulation of NRDE-3. Therefore, we concluded that siRNAs could accumulate in the pericentrosomal region in C. elegans and suggested that the peri-centrosomal region may also be a platform for RNAi-mediated gene regulation.

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油炸条件对猪里脊品质和杂环胺形成的影响

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食品与发酵工业 2025年第3期51卷 171-181页

作者：黄欣娅戴宏杰郭婷周鸿媛张宇昊马良西南大学食品科学学院重庆400715 川渝共建特色食品重庆市重点实验室重庆400715 发光分析与分子传感教育部重点实验室重庆400715 国家市场监管重点实验室(调味品监管技术) 重庆401121 农业农村部柑橘类果品质量安全重点实验室重庆400715

猪肉含有丰富的膳食蛋白质、维生素及矿物质等营养素,食用历史悠久,在中国的饮食文化中占据举足轻重的地位。高蛋白食品在高温热加工过程中易产生杂环胺类风险因子,国内外对于禽肉及牛肉中杂环胺的产生已有丰富研究,但是关于猪里脊在高... 详细信息

猪肉含有丰富的膳食蛋白质、维生素及矿物质等营养素,食用历史悠久,在中国的饮食文化中占据举足轻重的地位。高蛋白食品在高温热加工过程中易产生杂环胺类风险因子,国内外对于禽肉及牛肉中杂环胺的产生已有丰富研究,但是关于猪里脊在高温油炸加工条件下杂环胺的变化规律研究较为缺乏。该文研究了不同油炸温度(150、160、170、180和190℃)和油炸时间(1、2、3、4和5 min)对油炸猪里脊的pH、烹饪损失、水分、颜色、质构、水分分布、油炸温度、感官评价以及杂环胺(heterocyclic aromatic amines,HAA)含量的影响。结果表明,随着油炸温度和油炸时间的增加,猪里脊中的水分含量、自由水含量、b^(*)和L^(*)显著降低,表面中心温度、烹饪损失、a^(*)、硬度、内聚性、油脂含量和HAA含量增加。在170℃下油炸3 min的熟度、质构品质相对较好,且HAA含量相对较低。在相对较高油炸温度(180~190℃)和较长油炸时间(4~5 min)下,猪里脊中的HAA含量相对较高。相关性分析表明,杂环胺生成与油炸温度、时间和猪里脊品质显著相关。综合分析不同油炸参数对猪里脊品质和HAA的含量的影响,在160~170℃下油炸2~3 min是制备油炸猪里脊的最适条件。该文为消费者和餐饮机构提供关于肉类油炸时间和温度的建议,以确保油炸猪里脊产品的高质量和安全性。

关键词：油炸猪里脊杂环胺低场核磁共振质构红外热成像

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Synthesis of Acrylate Microemulsion Modified by Alkoxy Silane

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Journal of Wuhan University of Technology(Materials Science) 2008年第2期23卷 212-217页

作者： SUN Zhijuan ZHANG xinya huang Hong FU Heqing CHEN Huanqin Research Institute of Chemical Engineering South China University of Technology Guangzhou 510641 China

An acrylate emulsion was modified by adding vinyltriisopropoxy silane （trade name C-1706）. By adding the multiple emulsifier which consists of an anionic emulsifier, sodium dodecyl benzene sulfonate （SDBS） and nonionic emulsifier, octyl phenolic divinyl oxide （OP-10）, the acrylosilane microemulsion was synthesized by seeded emulsion polymerization. The influential factors including the kind and the adding amount of emulsifiers and the monomer variety of alkoxy silane and the added methods which influence on the properties of the microemulsion were investigated. It is found that SDBS and OP-10 as multiple emulsifiers with mass ratio of 1：1 and the adding amount of 2.5%-3.5% can act on co-effect for emulsion polymerization. The C-1706 possesses bulky isopropoxy substituent that can reduce hydrolysis reactivity during the polymerization process, so as to not only make the process smoothly but also advance the store stability of the emulsion. Moreover, the latter-addition mode of C-1706 can restrain its hydrolysis activity and polycondensation reaction during the polymerization process of the emulsion. The structure, the film cross section, the particle size and its distribution of the microemulsion were analyzed by the Fourier Transform Infrared Ray Spectrum （FTIR）, Scanning Electron Microscopy （SEM） and a particle size analyzer, respectively. The results show that the particle diameter of the modified microemulsion can be controlled between 50 and 70 nm and its film hardness is 7.3. Only adding 1.5% of C-1706 into the system of emulsion polymerization can apparently improve the weathering resistance of the microemulsion, which undergo degradation with chromatism（△E） is 1.6 after 3 600 hours of QUV-aging.

关键词： alkoxy silane acrylosilane microemulsion modification properties

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Iron-bound organic carbon dynamics in peatland profiles:The preservation equivalence of deep and surface soil

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Fundamental Research 2023年第6期3卷 852-860页

作者： xinya huang Xinwei Liu Liangshuai Chen Yanfen Wang Huai Chen Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization&Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China Zoige Peatland and Global Change Research Station Chinese Academy of SciencesHongyuan 624400China CAS Center for Excellence in Tibetan Plateau Earth Sciences Chinese Academy of Sciences(CAS)Beijing 100101China. University of Chinese Academy of Sciences Beijing 100049China

More than half of the carbon pools in peatlands are stored in the soil layers below 30 cm,yet little is known about the carbon stabilizing factors at these *** iron oxide minerals are considered to be important for stabilizing organic carbon(OC),their role in the preservation of OC in peatlands,especially in the deep layers,is poorly ***,we collected 1 m soil profiles from six peatlands in Central and West China to quantitatively study the vertical distribution characteristics of iron-bound OC(Fe-bound OC),and the influencing physicochemical properties of the *** results showed that the content of reactive iron(FeR)was enriched in the top layer and decreased gradually with *** Fe-bound OC was positively correlated with FeR,its concentration did not decrease with depth in the peat *** were no obvious trends in the distributions of FeR and Fe-bound OC with water level fluctuations in the peat *** addition,the proportion of Fe-bound OC to soil organic carbon in the deep peat(31 to 100 cm)was equivalent to that in the surface peat(0 to 30 cm),indicating that iron oxide mineral provides comparable protection of OC in both *** to upper estimates of global peatland carbon storage(612 Pg),it could be predicted that 23.81±11.75 Pg of OC is protected by association with *** results indicated that iron oxide minerals are the effective"rusty sink"of OC sequestration in peatland,and a key factor for its long-term *** results from this study make a valuable contribution to the carbon dynamics knowledgebase for peatlands,and provide a basis for improved predictive simulations.

关键词： Iron-bound organic carbon Peatland Soil profile Reactive Fe Carbon preservation

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Influence of Carboxyl Groups on the Particle Size and Rheological Properties of Polyacrylate Latices

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Journal of Wuhan University of Technology(Materials Science) 2010年第3期25卷 492-498页

作者：张心亚 School of Chemistry and Chemical Engineering South China University of Technology

The PAL was synthesized with BA,MMA and some monomers containing carboxyl groups（for example,acrylic acid（AA） and methacrylic acid（MAA）） as co-monomers by semi-continuous seeded emulsion polymerization *** influences of alkalinization temperature,the feeding manner of AA or MAA on the particles size,rheological properties and carboxyl distribution of the latex were discussed,and the rheological mechanism was *** experimental results show that the PAL system has preferable viscosity and particle size when the alkalinization temperature is 50 ℃.Different distribution of carboxyl group in the particles and different resultant rheological properties are obtained by different feeding manner of AA or MAA into the *** TEM images show that the particle is a smooth globe with carboxyl group concentrating on the surface and stabilized with electric double layer and nonionic adsorbed *** concentration of carboxyl functional group on the surface of particles can be achieved by the specific polymerization *** rheologyical properties are determined by accretion of particle volume and variation of the two phase volume ratio resulted from the carboxyl group spreading layer.

关键词： polyacrylate latices acrylic acid methacrylic acid rheological properties

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Preliminary Research on Plant Community and Vegetation Restoration in Different Vegetation Succession Species on a Post-Seismic Landslide in Hongchun Gully, China

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Journal of Geoscience and Environment Protection 2025年第1期13卷 89-102页

作者： Yuanyuan Wang Mei Wang Nuosi Li xinya Zhou Youyou huang Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education) China West Normal University Nanchong China

After the Wenchuan earthquake on May 12, 2008 and the rainstorm and debris flow on August 14, 2010, the vegetation in Hongchun Gully, Yingxiu town is gradually recovering naturally, and the plant community is gradually undergoing community succession. Based on the field vegetation survey data of 46 quadrats in 5 field plots, this study comprehensively analyzed the plant community, species number changes and vegetation characteristics of Hongchun Gully in Yingxiu town at different stages of vegetation succession after the earthquake by using the unified method. The results show that: 1) There are 61 families, 96 genera and 110 species of plants on the Hongchun Gully landslide. Among them, Cunninghamia lanceolata is the most widely distributed, and together with Cryptomeria japonica, it constitutes the main dominant species in the tree layer;The dominant species in shrub layer are Rubussp., Hydrangea strigosa, Rubus tephrodes, etc. The dominant species in herb layer are Artemisia argyi, Stellaria media and Senecio scandens. 2) The vegetation restoration in Hongchun Gully is relatively good, with herbs as the main species in the early stage of succession, shrubs and herbs as the main species in the middle stage of succession and trees as the main species in the late stage of succession;And the longer the succession time, the better the vegetation restoration and the richer the species. 3) Vegetation succession is related to the succession time, and the succession is always in the direction of strong adaptability. The study provides important data reference for further discussing the natural restoration and succession process and mechanism of plant communities on the damaged landslide formed after the earthquake, and provides theoretical basis for vegetation restoration and ecological reconstruction in the hardest hit areas in southwest China after the earthquake.

关键词： Landslide Community Succession Vegetation Restoration Dominant Species Earthquake Yingxiu Town

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Poly（U） polymerase PUP-1 can act as a new suppressor of siRNA to control rRNA homeostasis in ***

Poly（U） polymerase PUP-1 can act as a new suppressor of si...

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第五届中国秀丽线虫大会

作者： Yun Wang xinya huang Chenchun Weng Xiangyang Chen Chengming Zhu Shouhong Guang School of Life Sciences University of Science and Technology of China

＜正＞For a long time,small rRNA sequences have been widely treated as nonspecific degradation products and neglected as garbage *** recent years,our team has identified antisense ribosomal siRNAs（risiRNAs） that dow...

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广义延拓逼近法在GPS卫星精密星历内插中的精度分析

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甘肃科技纵横 2019年第7期48卷 59-62页

作者：黄馨娅西北师范大学数学与统计学院

为了得到更高精度的GPS卫星在任意时刻的位置信息,需要利用合适的数学模型去内插精密星历。首先,说明广义延拓逼近法、拉格朗日多项式插值法及切比雪夫拟合法的原理;其次,分别利用这三种方法处理GPS精密星历;最后,通过对比分析三种方法... 详细信息

为了得到更高精度的GPS卫星在任意时刻的位置信息,需要利用合适的数学模型去内插精密星历。首先,说明广义延拓逼近法、拉格朗日多项式插值法及切比雪夫拟合法的原理;其次,分别利用这三种方法处理GPS精密星历;最后,通过对比分析三种方法所得结果误差的最大值与平均值发现:使用广义延拓逼近法所得结果的精度略高于拉格朗日多项式插值法和切比雪夫多项式拟合法。

关键词：广义延拓逼近法 GPS卫星精密星历内插精度分析

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660MW旋流对冲燃煤锅炉燃烧过程的数值模拟及结渣分析

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动力工程学报 2014年第9期34卷 673-677页

作者：邓念念周臻肖祥黄歆雅长沙理工大学能源与动力工程学院长沙410004

在100%、75%和50%负荷下以及不同煤粉细度下对某660MW旋流对冲燃煤锅炉进行燃烧过程数值模拟计算,分析了影响该类型锅炉结渣的因素.结果表明:100%负荷下炉膛左右墙19~24m高度截面中间区域出现结渣的概率最大,75%负荷下炉膛左右墙21~23m... 详细信息

在100%、75%和50%负荷下以及不同煤粉细度下对某660MW旋流对冲燃煤锅炉进行燃烧过程数值模拟计算,分析了影响该类型锅炉结渣的因素.结果表明:100%负荷下炉膛左右墙19~24m高度截面中间区域出现结渣的概率最大,75%负荷下炉膛左右墙21~23m高度截面中间区域最有可能出现结渣情况,50%负荷下炉内出现结渣的可能性最小;当煤粉细度为10μm时,炉膛左右墙出现结渣的区域为23~28m高度截面的中间区域,且煤粉细度减小会加快煤粉着火速度,提高炉膛整体温度,增大炉内可能出现结渣情况的区域面积.

关键词：旋流对冲燃煤锅炉结渣炉膛温度煤粉细度数值模拟

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柑橘采后青霉菌的侵染及产毒机制的研究进展

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食品工业科技 2024年第6期45卷 389-395页

作者：黄欣娅郭婷戴宏杰张宇昊马良西南大学食品科学学院重庆400715 川渝共建特色食品重庆市重点实验室重庆400715 农业农村部柑橘类果品质量安全重点实验室重庆400715 发光分析和分子传感教育部重点实验室重庆400715

柑橘是中国的第一大水果作物,柑橘采后青霉病是影响柑橘品质安全的重大病害之一,还会造成果品霉菌毒素污染的风险。目前研究主要集中于***的侵染机制,而重点青霉菌对柑橘的感染和产毒机制有着密切的联系,产生的真菌毒素会对食品安全造... 详细信息

柑橘是中国的第一大水果作物,柑橘采后青霉病是影响柑橘品质安全的重大病害之一,还会造成果品霉菌毒素污染的风险。目前研究主要集中于***的侵染机制,而重点青霉菌对柑橘的感染和产毒机制有着密切的联系,产生的真菌毒素会对食品安全造成严重威胁,基于此,本文针对柑橘采后主要青霉菌,综述其侵染和致病机制、产毒机制,分析目前柑橘产业采后青霉病的研究趋势和方向,以期降低真菌毒素污染风险,推动柑橘产业“提质增效”,为柑橘品质提升和产业发展提供研究新思路。

关键词：青霉病柑橘 P.digitatum 真菌毒素

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