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液体活检技术在结直肠癌诊疗领域的应用

中华结直肠疾病电子杂志 2024年第2期13卷 135-140页

作者：赵迪杨鑫鑫孟宏学哈尔滨医科大学肿瘤医院病理科精准医学中心150081

结直肠癌是一种常见的消化道系统肿瘤,我国结直肠癌发病率在所有恶性肿瘤中居第二位,死亡率居第四位。目前结直肠癌的诊断主要依赖组织活检,但组织活检存在侵入性、技术要求相对高及可重复性差等弊端。液体活检技术是一种新型检测技术,... 详细信息

结直肠癌是一种常见的消化道系统肿瘤,我国结直肠癌发病率在所有恶性肿瘤中居第二位,死亡率居第四位。目前结直肠癌的诊断主要依赖组织活检,但组织活检存在侵入性、技术要求相对高及可重复性差等弊端。液体活检技术是一种新型检测技术,可通过各种体液,主要是外周血对各类癌症进行诊断,因其敏感度高、依从性强、可重复性好及非侵入性而备受瞩目,有很强的临床应用价值。目前,液体活检技术主要应用于癌症早期筛查、伴随诊断、病程监测及预后评估领域,是十分有前景的结直肠癌辅助检查手段。近期,液体活检技术即微小残留病变(MRD)的检测在结直肠癌诊疗中取得了突破性的进展。本文旨在汇总目前液体活检技术在结直肠癌的早期筛查、伴随诊断、病程监测及预后评估四大方面的作用。

关键词：结直肠肿瘤液体活检精准诊疗

基于《雷公炮炙论》的白矾蜂巢制炮制工艺研究

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中草药 2024年第12期55卷 4008-4019页

作者：杨辛欣黄梓骏洪禹昕康爽洪玉书王楚盈于澎长春中医药大学吉林长春130117

目的传承古法,建立白矾蜂巢制的炮制工艺。方法以炮制品外观性状、硫酸铝钾的含量、蜂巢制白矾粉末色度、溃疡损伤程度为指标,采用单因素结合星点设计-响应面法对白矾蜂巢制炮制工艺进行考察。最后采用扫描电镜法和红外光谱法对白矾、... 详细信息

目的传承古法,建立白矾蜂巢制的炮制工艺。方法以炮制品外观性状、硫酸铝钾的含量、蜂巢制白矾粉末色度、溃疡损伤程度为指标,采用单因素结合星点设计-响应面法对白矾蜂巢制炮制工艺进行考察。最后采用扫描电镜法和红外光谱法对白矾、蜂巢制白矾及煅制品枯矾进行鉴别与质量控制。结果确定了白矾蜂巢制的最佳炮制工艺为取白矾样品,置于煅药炉中煅烧,加热温度为(587±5)℃,加热时间为60 min,取出,趁热加入0.6倍量蜂巢,保持温度继续加热180 min,取出晾凉后,研成粉末状,用麻纸包裹,埋于深12~15 cm的土壤中,土壤温度为20~25℃,土壤水分为20%~40%,坑埋时长为12 h,即得。白矾炮制前后微观形态变化显著,炮制品粒子粒度变小,表面结构更粗糙,疏松多孔结构明显,蜂巢制白矾与煅制品枯矾相比表面的碎屑呈圆柱状或椭圆状,加入蜂巢使晶体结构出现融合现象,晶体边缘更加圆钝。白矾的红外光谱特征峰为618、918、1113、1670、3441 cm^(−1),蜂巢制白矾的特征峰为472、617、688、1121、1242、1640、3446 cm^(−1),枯矾的特征峰为477、618、688、1112、1645、3458 cm^(−1),3者峰形和峰强度存在明显差异,且相似度分析和聚类分析结果均显示3者具有显著性差异。结论白矾蜂巢制工艺遵古继承了《雷公炮炙论》中的传统炮制方法,保留了传统煅制的特点,量化了关键操作技术点温度以及前后时间等重要参数,经验证工艺条件稳定、合理、可行性强,为大工业的生产提供了理论基础。扫描电镜和红外光谱分析法可以从微观形态层面和成分结构变化2个方面对白矾生品、蜂巢制白矾和枯矾进行鉴别与质量控制。

关键词：雷公炮炙论白矾蜂巢制炮制工艺星点设计-响应面法扫描电镜红外光谱分析法

Monoamine alterations and rotational asymmetry in a rat model of Parkinson's disease following lateral ventricle transplantation of human amniotic epithelial cells

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Neural Regeneration Research 2009年第12期4卷 1007-1012页

作者： Shouru Xue xinxin yang Wanli Dong Guozhen Hui Lihe GUO Department of Neurology First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Department of Neurosurgery First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai China Shanghai Celstar Institute of Biotechnology Shanghai 201203 China

BACKGROUND： Human amniotic epithelial cells （HAECs） can differentiate into neurons, astrocytes and oligodendrocytes. They biologically secrete many active neurotrophins and have the capacity to metabolize dopamine enzymes. These features underlie a theoretical basis for the treatment of Parkinson＇s disease （PD）. OBJECTIVE： To investigate the survival and differentiation of transplanted HAECs in the lateral ventricle of PD model rats, and to explore its effect on circling behavior, as well as levels of dopamine （DA）, the metabolite homovanillic acid, dihydroxyphenyl acetic acid, 5-hydroxyindoleacetic acid, and 5-hydroxytryptamine in the striatum. DESIGN, TIME AND SETTING： A randomized, controlled, animal study was performed at the Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, and Shanghai Celstar Institute of Biotechnology from May 2007 to December 2008. MATERIALS： HAECs were derived from the placental chorion following caesarean delivery at the Shanghai International Matemal and Child Health Hospital. 6-hydroxydopamine （6-OHDA）, and mouse anti-human Vimentin monoclonal antibody were purchased from Sigma, USA; mouse anti-human nestin and tyrosine hydroxylase （TH） monoclonal antibodies were purchased from Chemicon, USA. METHODS： A total of 114 healthy, adult, Sprague Dawley rats were randomly assigned to two groups： PD model [n = 90, stereotactic microinjection of 2 μL 6-OHDA （3.5 μg/uL） into the striatum] and control （n = 24, no treatment）. The 51 successful PD model rats were randomly divided into 3 subgroups （n = 17）： HAEC, PBS, and model. The HAEC and PBS groups were respectively injected with 10 μL PBS solution containing 1 × 10^5/mL HAECs or 10 pL PBS into the lateral ventricle. The model group was not treated. MAIN OUTCOME MEASURES： TH protein expression in the striatum was evaluated by immunohistochemistry 5 weeks after HAEC transplantation. At 10 weeks, HAEC survival in the lateral ventric

关键词： human amniotic epithelial cells Parkinson's disease model lateral ventricle cell transplantation dopamine

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袋装螺蛳粉自动化包装生产线设计

机械工程师 2024年第3期 59-64页

作者：杨新新林贤坤冷臻广西科技大学机械与汽车工程学院广西柳州545000

随着袋装螺蛳粉销量与日俱增,已有的自动化程度较低的袋装螺蛳粉自动化生产线难以满足越来越多的订单需求,为了实现更高产量、更高效率,设计了一条袋装螺蛳粉自动化包装生产线,该生产线采用模块化设计,设计了各模块的机械机构,使用PLC... 详细信息

随着袋装螺蛳粉销量与日俱增,已有的自动化程度较低的袋装螺蛳粉自动化生产线难以满足越来越多的订单需求,为了实现更高产量、更高效率,设计了一条袋装螺蛳粉自动化包装生产线,该生产线采用模块化设计,设计了各模块的机械机构,使用PLC设计生产线的控制系统。该自动化包装生产线可实现袋装螺蛳粉的自动化上料、分拣、包装等环节。

关键词：自动化产线 PLC 螺蛳粉包装

Phase-tunable equiatomic and non-equiatomic Ti-Zr-Nb-Tahigh-entropy alloys with ultrahigh strength for metallic biomaterials

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Journal of Materials Science & Technology 2022年第22期117卷 196-206页

作者： Tao Xiang Peng Du Zeyun Cai Kun Li Weizong Bao xinxin yang Guoqiang Xie School of Materials Science and Engineering Harbin Institute of Technology(Shenzhen)Shenzhen 518055China State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001China

The contradiction between the strength and ductility of metallic materials is a major scientific problemthat has been researched for a long time. Dual-phase equiatomic and non-equiatomic Ti-Zr-Nb-Ta highentropy alloys (HEAs) with super-high strength and excellent ductility have been successfully developedvia mechanical alloying (MA) combined with spark plasma sintering (SPS) technology. This is adjustedby altering the atomic ratios of the different phases. X-ray diffraction (XRD) and transmission electronmicroscopy (TEM) were performed to confirm the dual-phase microstructure. After the SPS process, theaverage grain size of the aforementioned equiatomic Ti_(25)Zr_(25)Nb_(25)Ta_(25) HEAs (134 ± 50 nm) evaluated byelectron back-scattering diffraction (EBSD) is smaller than that of the Ti-Zr-Nb-Ta HEAs (150 μm), whichwere fabricated using arc melting. According to the Hall-Petch formula, the grain boundary strengthening contribution in the Ti-Zr-Nb-Ta system is 33-fold higher than those fabricated using the arc-meltingprocess. When the alloy phase comprises the equivalent dual-phase, equiatomic Ti_(25)Zr_(25)Nb_(25)Ta_(25) HEAshave good comprehensive performance compared to non-equiatomic Ti-Zr-Nb-Ta HEAs prepared usingthe same process. The yield strength of equiatomic Ti_(25)Zr_(25)Nb_(25)Ta_(25) HEAs (2212 ± 38 MPa) is two-foldhigher than that of Ti-Zr-Nb-Ta HEAs (1100 ± 90 MPa) fabricated via arc melting. This can be attributed tothe ultra-fine grain size. Notably, the equiatomic Ti_(25)Zr_(25)Nb_(25)Ta_(25) HEAs possess approximately the samebiocompatibility as commercial pure Ti (CP-Ti), indicating that the equiatomic Ti_(25)Zr_(25)Nb_(25)Ta_(25) HEAs areprovided with a possibility as an advanced biomaterial for the applications of the medical field.

关键词： Equiatomic Nb_(25)Ta_(25)Ti_(25)Zr_(25)HEAs Ultra-fine grain Phase-tunable Mechanical properties Biocompatibility

Feasibility of probing solid state nuclear tracks by thermal analysis method

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Chinese Science Bulletin 2007年第8期52卷 1013-1018页

作者： yang TongSuo ZHOU Bing yang xinxin HE ShaoRong HENG ShuYun YUAN SunSheng Experiment Center of Xijing College Xi'an 710123 China Department of Computer Science and Engineering Northeastern University Qinhuangdao 066004 China Xi'an Modern Chemistry Research Institute Xi'an710065 China

The feasibility of probing solid state nuclear tracks by thermal analysis method is discussed both theoretically and *** is made between the thermal analysis method and the optical microscope method,and it is demonstr... 详细信息

关键词：固体核径迹探测技术可行性热分析法

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大直径双孔盾构隧道下穿既有道路沉降预测

东华大学学报（自然科学版） 2024年第3期50卷 137-144页

作者：张家贺刘书荣刘艳涛杨超杨欣欣董亚博罗明福王斌彬华谦谦中国电建铁路建设投资集团有限公司北京东华大学环境科学与工程学院上海

盾构隧道掘进会对地层产生一定的扰动,引起地表沉降、隆起变形。为预测双孔隧道下穿既有道路以及穿越粉细砂地层时引起的地表沉降,依托雄安至大兴国际机场快线盾构隧道工程,并结合工程监测数据,建立双孔Peck公式。研究表明:针对左右线... 详细信息

盾构隧道掘进会对地层产生一定的扰动,引起地表沉降、隆起变形。为预测双孔隧道下穿既有道路以及穿越粉细砂地层时引起的地表沉降,依托雄安至大兴国际机场快线盾构隧道工程,并结合工程监测数据,建立双孔Peck公式。研究表明:针对左右线先后下穿既有道路的工程,其断面地表沉降呈“W”形,两隧道轴向的沉降量最大;后线掘至相同断面时,会加大先行掘进的地表沉降量;基于Peck公式和实测数据,建立预测双孔下穿既有道路和穿越粉细砂地层时断面地表沉降的双孔Peck公式,为类似地层和工程的隧道工程提供理论依据。

关键词：大直径双孔盾构隧道下穿既有道路断面地表沉降双孔Peck公式

Facile fabrication of MoP nanodots embedded in porous carbon as excellent anode material for potassium-ion batteries

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Journal of Energy Chemistry 2021年第3期30卷 571-578页

作者： Zhanheng Yan Zhongyuan Huang Haihui Zhou xinxin yang Songlin Li Wenlong Zhang Fei Wang Yafei Kuang State Key Laboratory for Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082HunanChina State Key Laboratory of Powder Metallurgy Central South UniversityChangsha 410083HunanChina Advanced Catalytic Engineering Research Center of the Ministry of Education China

Molybdenum phosphide(MoP),owing to its abundant reserve and high theoretical capacity,is regarded as a promising anode material for potassium-ion ***,it still suffers from the problems of acute volume expansion and weak diffusion *** study reports a simple method to synthesize a composite of molybdenum phosphide and porous carbon(MoP@PC)through simple mixing and annealing *** the MoP@PC,lots of MoP nanodots with an average diameter of about 4 nm uniformly embedded in the petal-like porous *** MoP@PC shows reversible capacities of 330 mAh g^(-1) at100 mA g^(-1) after 100 cycles,and ultra-long cycling stability with a capacity of 240 mAh g^(-1) after 1000 cycles at 1 A g^(-1) and 161 mAh g^(-1) after 1000 cycles at 5 A g^(-1).The structure of MoP@PC after charging-discharging cycles is also investigated by high resolution transmission electron microscope(HRTEM)and the result shows that MoP can still maintain the nanodot morphology without any agglomeration after 1000 cycles at 5 A g^(-1).The storage mechanism of potassium ions was studied as well,which reveals that MoP and potassium ion have a conversion reaction.

关键词： MoP Nanodot Anode Potassium-ion batteries

甲型流感病毒感染老年小鼠支气管肺泡灌洗液中细胞因子变化与精氨酸代谢的相关性研究

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中华实验和临床病毒学杂志 2024年第4期38卷 422-431页

作者：周娟娟刘诗洋李雪杨欣欣杨俊连罗声栋陈威巍许文王福生蚌埠医科大学研究生院蚌埠233030 中国人民解放军总医院第五医学中心感染病医学部研究所北京100039 滨州医学院药学院烟台264003

目的探究精氨酸代谢在甲型流感病毒(influenza A virus,FluA)感染引起的炎症反应中的作用。方法采用FluA滴鼻感染18月龄老年小鼠,于感染后第6天采集肺组织和支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)。采用Luminex多因子检... 详细信息

目的探究精氨酸代谢在甲型流感病毒(influenza A virus,FluA)感染引起的炎症反应中的作用。方法采用FluA滴鼻感染18月龄老年小鼠,于感染后第6天采集肺组织和支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)。采用Luminex多因子检测法分析BALF中细胞因子水平,采用靶向代谢组学的方法分析BALF中代谢物的变化情况,并对细胞因子与代谢物之间的相关性进行分析。以FluA感染复数(multiplicity of infection,MOI)为1的剂量感染巨噬细胞,并加入不同浓度的精氨酸培养24 h。分别采用实时荧光定量RT-PCR法(real time fluorescence quantitative RT-PCR,qRT-PCR)和流式微球技术(cytometric bead array,CBA)检测白介素(interleukin,IL)-1β、IL-6、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)和IL-10的mRNA和蛋白表达量。结果与对照组相比,FluA感染组老年小鼠BALF中肺表面活性蛋白D(surfactant protein D,SP-D)、TNF-α、单核细胞趋化蛋白1(monocyte chemotactic protein-1,MCP-1)、IL-1α、IL-6、IL-10、S100钙结合蛋白(recombinant S100 calcium binding protein,S100)A9、干扰素诱导蛋白10(interferon inducible protein 10,IP-10)、巨噬细胞炎症蛋白1α(macrophage inflammatory protein-1α,MIP-1α)、基质金属蛋白酶9(matrix metalloprotein-9,MMP9)、补体因子D的水平显著升高,精氨酸代谢通路中精氨酸、天冬氨酸、瓜氨酸、谷氨酸、鸟氨酸、脯氨酸、肌酸和肌氨酸水平上调,且大部分升高的细胞因子水平与精氨酸相关代谢物呈显著正相关。FluA感染后加入精氨酸可以使巨噬细胞中IL-1β、IL-6、TNF-α的表达水平显著降低,而IL-10的水平升高。结论精氨酸可以降低FluA感染诱导的巨噬细胞炎症反应,提示其可能成为FluA感染引起的肺部重症炎症的潜在干预靶点。

关键词：甲型流感病毒支气管肺泡灌洗液细胞因子精氨酸代谢