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Aldehyde oxidase mediated enantioselective metabolic health risk of dinotefuran

Science China Chemistry 2024年第6期67卷 2079-2091页

作者： Liwei xu xinxin xu Hua Kuang Chuanlai xu Xiaoling Wu State Key Laboratory of Food Science and Resources Jiangnan UniversityWuxi214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and Technology Jiangnan UniversityWuxi214122China Collaborative Innovation center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityWuxi214122China

Chiral pollutants often pose significant differential environmental health *** this study,the biotransformation of chiral dinotefuran(DIN)and its enantioselective metabolic toxicity mechanisms have been systemically ***,reversedphase chromatography-high resolution mass spectrometry was developed to quantify the content of DIN R/S chiral enantiomer with pg level sensitivity,revealing a lower elimination rate constant(K_(e))of S-DIN(0.730 h^(-1))than R-DIN(0.746 h^(-1)).Secondly,the interaction mechanism between DIN metabolism and important endogenous bioactive molecules,such as aldehyde oxidase(AOX)and neurotransmitters,was *** DIN nitro-group was converted into a guanidine group by the reducing site of nearby flavin adenine dinucleotide(FAD)in AOX with the preferred higher affinity of ***,the endogenous tryptophan(Trp)aldehyde metabolic intermediate,5-hydroxyindoleacetaldehyde(5-HIAL),provides a persistent electron donor for DIN reduction via the oxidation-catalyzed site in AOX,resulting in remarkable up-regulation of monoamine neurotransmitters such as serotonin and ***,the higher level of neurotransmitters further mediated dysregulation of oxylipin homeostasis via the serotonergic pathway,where S-DIN exhibited more pronounced liver lipid damage and environmental health risk with the accumulated lipid biomarkers,oxidized triglyceride(OxTG)and oxidized sphingomyelin(OxSM).This study elucidates the AOX-mediated enantioselectivity metabolic pathway of DIN,providing a new analytical method for chiral pollutants and paves the way for their health risk assessments.

关键词： neonicotinoid dinotefuran enantiomer aldehyde oxidase metabolomics health risk

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Hybrid battery integrated by Zn-air and Zn-Co3O4 batteries at cell level

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Journal of Energy Chemistry 2020年第10期29卷 375-383页

作者： Ning Liu Honglu Hu xinxin xu Qiang Wang Department of Chemistry College of ScienceNortheastern UniversityShenyang 110819LiaoningChina Key Laboratory of Electromagnetic Processing of Materials MOENortheastern UniversityShenyang 110819LiaoningChina

The construction of Zn based hybrid battery through the combination of Zn-air and Zn-Co3O4 batteries at cell level is a feasible strategy to integrate high voltage,specific capacity and energy density in one power supply *** Zn based hybrid battery,an efficient cathode material with high specific capacitance and excellent ORR,OER activities is a vital component,which determines its performance in great *** this work,with Co based coordination polymer as precursor,oxygen vacancy-rich Co3 O4 based cathode material is *** this material Co3O4 particles with the size about 20 to 35 nm reside evenly in mesoporous carbon matrix doped by nitrogen *** OER,the overpotential of this cathode material is merely 330 m *** ORR proceeds with a typical four electron process with half wave achieving 0.76 *** charge/discharge at 1 A·g^-1,specific capacitance of this cathode material is 254.4 mAh·g^-*** current density increases to 20 A·g^-1,the specific capacitance still arrives at 122.5 mAh·g^-1 with nearly 50%*** on attractive performance of this cathode material,Zn based hybrid battery is *** discharge at 1 m A·cm-2,it presences two voltage platforms at 1.71 and 1.14 *** this situation,specific capacitance reaches 790 m Ah·g^-1 with energy density 928 Wh·kg^-*** battery shows promising stability after 300-cycle continuous test.

关键词： Zn based battery Hybrid battery Oxygen vacancy Electrocatalysis Co3O4

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TiO_2-loaded activated carbon fiber:Hydrothermal synthesis,adsorption properties and photo catalytic activity under visible light irradiation

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Particuology 2014年第3期12卷 38-43页

作者： Hao Meng Wei Hou xinxin xu Junli xu Xia Zhang Department of Chemistry College of ScienceNortheastern University

TiO2-loaded activated carbon fibers （ACF） were prepared by a hydrothermal method. The samples were characterized by scanning electron microscopy （SEM）, X-ray diffraction （XRD）, Fourier transform infrared （FTIR） spectrometry and UV-vis diffuse reflectance spectra （DRS）. SEM images showed that the TiO2 nanoparticles were deposited on the surface of ACF, and the particle size and loading amount of TiO2 were varied by changing the initial concentration of tetrabutyl titanate （TBOT）. The results of an ash experiment showed that the loading amounts of TiO2 were 18.4%, 43.3%, 52.5%, 75.1%, and 91.1% for initial concentrations of TBOT of 0.07,014, 0.21,0.28, and 0.35 tool/L, respectively, Physical interactions played an important role in the formation of TiO2/ACF composite fibers that absorb UV and visible light. Compared with those of ACF, improved adsorption and photocatalytic activity toward Rhodamine B （RhB） were observed for TiO2/ACF composite fiber. The Rhodamine B could be removed efficiently by TiO2/ACF composite fibers, and the TiO2 loading amount had a significant effect on the photocatalytic activity of TiO2/ACF composite fibers.

关键词： Activated carbon fiber (ACF) TiO2 nanoparticles Adsorption property Photocatalytic activity Hydrothermal synthesis

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Amplification of an MFS Transporter Encoding Gene penT Significantly Stimulates Penicillin Production and Enhances the Sensitivity of Penicillium chrysogenum to Phenylacetic Acid

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Journal of Genetics and Genomics 2012年第11期39卷 593-602页

作者： Jing Yang xinxin xu Gang Liu State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Gra-duate University of Chinese Academy of Sciences Beijing 1O0039China

Penicillin is historically important as the first discovered drug against bacterial infections in human. Although the penicillin biosyn- thetic pathway and regulatory mechanism have been well studied in Penicillium chrysogenum, the compartmentation and molecular transport of penicillin or its precursors are still poorly understood. In search of the genomic database, more than 830 open reading frames （ORFs） were found to encode transmembrane proteins of P. chrysogenum. In order to investigate their roles on penicillin production, one of them （penT） was selected and cloned. The deduced protein ofpenTbelongs to the major facilitator superfamily （MFS） and contains 12 transmembrane spanning domains （TMS）. During fermentation, the transcription of penT was greatly induced by penicillin precursors phenylacetic acid （PAA） and phenoxyacetic acid （POA）. Knock-down of penT resulted in significant decrease of penicillin production, while over-expression of penT under the promoter of trpC enhanced the penicillin production. Introduction of an additional penT in the wild-type strain of P. chrysogenurn doubled the penicillin production and enhanced the sensitivity of P. chrysogenum to the penicillin precursors PAA or POA. These results indicate that penT stimulates penicillin production probably through enhancing the translocation of penicillin precursors across fungal cellular membrane. Penicillin is historically important as the first discovered drug against bacterial infections in human. Although the penicillin biosyn- thetic pathway and regulatory mechanism have been well studied in Penicillium chrysogenum, the compartmentation and molecular transport of penicillin or its precursors are still poorly understood. In search of the genomic database, more than 830 open reading frames （ORFs） were found to encode transmembrane proteins of P. chrysogenum. In order to investigate their roles on penicillin production, one of them （penT） was selected and cloned. The deduced protein ofpen

关键词： Penicillium chrysogenum pen Penicillin production Phenylacetic acid MFS transporter

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Polyoxometalate derived p-n heterojunction for optimized reaction interface and improved HER

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Chinese Chemical Letters 2023年第3期34卷 494-497页

作者： Xiaoli Cui Yunmeng Sun xinxin xu Department of Chemistry College of ScienceNortheastern UniversityShenyang 110819China Institute for Frontier Technologies of Low-Carbon Steelmaking Northeastern UniversityShenyang 110819China

MoS_(2) is a typical electrocatalyst for hydrogen evolution reaction(HER),but the HER activity is spoilt by intensive adsorption towards H^(*),which requires further *** n-type MoS_(2),the construction of p-n heterojunction with p-type MoO_(3) can reverse this situation,because inner electronic field in p-n heterojunction can facilitate H^(*) *** on this hypothesis,p-n heterojunction is built between MoS_(2) and MoO_(3) with polyoxometalate compound as *** obtained MoO_(3)/MoS_(2) exhibits excellent HER activity,which only requires 68 mV to obtain 10 mA/cm^(2).With MoO_(3)/MoS_(2) as cathode material and Zn slice as anode,Zn-H^(+)battery is *** open circuit voltage achieves 1.11 V with short circuit current 151.4 mA/cm^(2).The peak power density of this Zn-H^(+) battery reaches 47.6 mW/cm^(2).When discharge at 10 mA/cm^(2),the specific capacity and energy density reach 728 mAh/g and 759 Wh/*** this process,H_(2) production rate of Zn-H^(+) battery achieves 364μmol/h with Faradic efficiency 97.8%.It realizes H_(2) production and electricity generation simultaneously.

关键词： Polyoxometalate p-n Heterojunction Electrocatalysis HER Zn-H~+battery

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Multiple detection of 15 triazine herbicides by gold nanoparticle based-paper sensor

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Nano Research 2022年第6期15卷 5483-5491页

作者： Lingling Guo xinxin xu Jing Zhao Shudong Hu Liguang xu Hua Kuang Chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China Department of Radiology Affiliated HospitalJiangnan UniversityWuxi 214122China

Triazine herbicides have been widely used in agriculture,but their residues can harm the environment and human *** help monitor these,we have developed an effective immunochromatographic strip test that can simultaneously detect 15 different triazines in grain samples(including ametryn,cyprazine,atraton,prometon,prometryn,atrazine,propazine,terbuthylazine,simetryn,trietazine,secbumeton,simazine,desmetryn,terbumeton and simetone).Based on our optimization procedure,the visual limit of detection(vLOD)for these triazines was found to be 2–10 ng/mL in assay buffer,and 0.02–0.1 mg/kg in grain *** different grain matrices including corn,brown rice,wheat,and sorghum were studied and the test results showed no significant differences between the 15 triazines analyzed using this *** test is simple,convenient,rapid,and low-cost,and could be an effective tool for primary screening of triazine residues in grain samples.

关键词： triazine herbicides immunochromatographic strip gold nanoparticles grain

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Gold-nanoparticle-based multiplex immuno-strip biosensor for simultaneous determination of 83 antibiotics

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Nano Research 2023年第1期16卷 1259-1268页

作者： Xianlu Lei xinxin xu Liqiang Liu Liguang xu Li Wang Hua Kuang Chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Antibiotic residues,generated by the irrational use of drugs and environmental pollution,have always been a great challenge to aquaculture ***,a quick,convenient,and performance-excellent way to detect antibiotic residues in aquaculture fish is urgently *** this study,a multiplex immunochromatographic strip biosensor based on gold nanoparticles was developed for the simultaneous detection of five classes of antibiotic residues(24β-lactam antibiotics,26 sulfonamides,five tetracyclines,24 quinolones,and four amphenicols)in aquaculture fish within 10 *** detection ranges of five representative antibiotics,penicillin G,sulfamethazine,tetracycline,enrofloxacin,and chloramphenicol,were 2.33–38.4,0.688–17.1,1.4–48.1,1.45–32.9,and 0.537–9.06μg/kg,*** accuracy and stability of these measurements were demonstrated by analyzing spiked fish samples,with recovery rates of 87.5%–115.2%and a coefficient of variation<9.5%.The cross-reaction rates,based on the five representative antibiotics,were 3.77%–202%forβ-lactams,3.95%–137%for sulfonamides,9.19%–100%for tetracyclines,4.9%–145%for quinolones,and 3.2%–100%for *** excellent testing performance of the biosensor strip to most of antibiotic residues in aquaculture fish ensures they meet the maximum residue limits required by countries or ***,this multiplex immunochromatographic strip biosensor is potentially applicable to the rapidly on-site determination of antibiotic residues in aquaculture fish.

关键词： antibiotic aquaculture biosensor immunochromatographic strip

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Quantitative assessment of the breast cancer marker HER2 using a gold nanoparticle-based lateral flow immunoassay

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Nano Research 2024年第6期17卷 5452-5460页

作者： Liya Ye xinxin xu Aihua Qu Liqiang Liu Chuanlai xu Hua Kuang International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Human epidermal growth factor receptor 2(HER2)is an important biomarker for detection and treatment of breast *** this study,we developed monoclonal antibodies against the extracellular domain(ECD)of HER2 and established a rapid and accurate lateral flow immunoassay(LFIA)for use in community medical *** gene sequence of human HER2-ECD was obtained from the National Center for Biotechnology Information(NCBI)to construct the expression ***2-ECD protein expressed in HEK293F cells was used to immunize BALB/c *** monoclonal antibodies were produced in mouse ascites and isolated by hybridoma cell *** were analyzed for purity by SDS-PAGE(sodium dodecyl sulphate-polyacrylamide gel-electrophoresis)and affinity was assessed by enzyme-linked immunosorbent assay(ELISA)while subtypes were detected using the commercial *** HER2-ECD test strip was prepared based on the sandwich method and evaluated using a portable detection *** affinity of the paired antibodies,4D8 and 8D9,both reached 1×108 L/*** antibodies specifically recognized the HER2-ECD protein in *** limit of detection(LOD)of the gold nanoparticle(AuNP)-based LFIA was 1.7 ng/mL with a detection range of 1.7-400 ng/mL,and the performance of the HER2-ECD strip correlated well with that of a Siemens chemiluminescent immunoassay(CLIA)*** conclusion,the paired antibodies were successfully prepared with high affinity and *** AuNP-based LFIA of HER2-ECD provides a fast and accurate method to detect the concentration of HER2-ECD in serum samples for clinical use in community medical institutions,and could contribute to determining the progress of the disease or the effectiveness of treatment.

关键词： human epidermal growth factor receptor 2 breast cancer monoclonal antibody quantitative lateral flow immunoassay

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Gold-based lateral-flow strip for the detection of penconazole in watermelon and cucumber samples

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Food Quality and Safety 2022年第2期6卷 257-264页

作者： Yang Liu xinxin xu Liqiang Liu Liguang xu Hua Kuang Chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxiChina International Joint Research Laboratory for Biointerface and Biodetection School of Food Science and TechnologyJiangnan UniversityWuxiChina

As a typical triazole fungicide,penconazole(PEN)is widely used in agriculture but has been proven to be *** this study,we designed a new hapten to prepare a highly sensitive and specific anti-PEN monoclonal antibody(mAb)and established a gold nanoparticle-based lateral-flow immunoassay(LFIA)for the detection of PEN residues in watermelon and *** 50%inhibitory concentration(IC_(50))of the mAb was 0.42 ng/mL and the LFIA strip had a visual limit of detection(vLOD)of 2.5 ng/g and a cut-off value of 10 ng/g in watermelon and *** calculated limit of detection(LOD)of the LFIA strip was 0.36 ng/g for watermelon and 0.29 ng/g for *** LFIA strip also gave a re-covery rate of 92.5%-109.0%for watermelon samples and 92.5%-106.7%for cucumber *** results using the LFIA strip are highly consistent with those seen using liquid chromatography-tandem mass *** our developed LFIA strip represents a potentially reliable tool for the rapid on-site screening for PEN in watermelons and cucumbers.

关键词： Penconazole monoclonal antibody lateral flow immunoassay watermelon cucumber

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A gold-based multiplex immunochromatographic sensor for detecting paclitaxel

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Nano Research 2024年第7期17卷 6420-6429页

作者： Xiaoqian Jiang Aihua Qu xinxin xu Hua Kuang Liqiang Liu Chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Paclitaxel(PTX),methotrexate(MTX),and 5-fluorouracil(5-FU)are commonly-used small molecule anti-tumor drugs for breast ***,drug resistance occurs with long-term treatment or excessive use,and the high concentrations of PTX,MTX,and 5-FU in patients may lead to side effects with dose-limiting toxicities,such as myelosuppression,hepatotoxicity,and peripheral ***,concentration monitoring of PTX,MTX,and 5-FU in clinical treatment is very *** prepared highly sensitive and specific monoclonal antibodies using several haptens,and established a multiple paper-based sensor utilizing optical signals of gold nanoparticles,which simultaneously detected PTX,MTX,and 5-FU in human plasma or urine with 10 min.A portable scanner for quantitative detection in plasma and urine samples was used,and the calculated limit of detection(cLOD)values were 0.002 and 0.142μg/mL for PTX,0.187 and 0.976 ng/mL for MTX,and 0.057 and 0.128μg/mL for 5-FU,*** the recovery test,the recovery rates and the coefficient of variation were 85.84%–108.81%and 1.23%–8.80%,respectively,indicating the reliability and accuracy of the multiple gold immunochromatographic strip(GIS).In addition,for the determination of collected clinical plasma samples,the correlation between the test data of the multiple GIS and liquid chromatography–tandem mass spectrometry(LC–MS/MS)was ***,the developed multiple GIS could be applied to the rapid detection of PTX,MTX,and 5-FU in different clinical samples.

关键词： paclitaxel methotrexate 5-fluorouracil immunochromatographic assay plasma urine

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