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Profiling of N6-methyladenosine methylation in porcine longissimus dorsi muscle and unravelling the hub gene ADIPOQ promotes adipogenesis in an m^(6)A-YTHDF1–dependent manner

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Journal of Animal Science and Biotechnology 2023年第3期14卷 950-965页

作者： Huanfa Gong Tao Gong Youhua Liu Yizhen wang xinxia wang Key Laboratory of Molecular Animal Nutrition Ministry of EducationCollege of Animal SciencesZhejiang UniversityHangzhou 310058People’s Republic of China Key Laboratory of Animal Nutrition and Feed Science in Eastern China Ministry of AgricultureCollege of Animal SciencesZhejiang UniversityHangzhou 310058People’s Republic of China

Background Intramuscular fat(IMF)content is a critical indicator of pork quality,and abnormal IMF is also relevant to human disease as well as aging.Although N6-methyladenosine(m^(6)A)RNA modification was recently found to regulate adipogenesis in porcine intramuscular fat,however,the underlying molecular mechanisms was still unclear.Results In this work,we collected 20 longissimus dorsi muscle samples with high(average 3.95%)or low IMF content(average 1.22%)from a unique heterogenous swine population for m^(6)A sequencing(m^(6)A-seq).We discovered 70genes show both differential RNA expression and m^(6)A modification from high and low IMF group,including ADIPOQ and SFRP1,two hub genes inferred through gene co-expression analysis.Particularly,we observed ADIPOQ,which contains three m^(6)A modification sites within 3’untranslated and protein coding region,could promote porcine intramuscular preadipocyte differentiation in an m^(6)A-dependent manner.Furthermore,we found the YT521-B homology domain family protein 1(YTHDF1)could target and promote ADIPOQ mRNA translation.Conclusions Our study provided a comprehensive profiling of m^(6)A methylation in porcine longissimus dorsi muscle and characterized the involvement of m^(6)A epigenetic modification in the regulation of ADIPOQ mRNA on IMF deposition through an m^(6)A-YTHDF1-dependent manner.

关键词： ADIPOQ Intramuscular fat N6-methyladenosine Pig YTHDF1

Simulation and fabrication of in vitro microfluidic microelectrode array chip for patterned culture and electrophysiological detection of neurons

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Nanotechnology and Precision Engineering 2024年第2期7卷 1-10页

作者： Yan Yang Shihong Xu Yu Deng Yaoyao Liu Kui Zhang Shiya Lv Longze Sha Qi Xu xinxia Cai Jinping Luo State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China

To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural cells.The chip was designed based on the specific structure of neurons and the requirements for detection and modulation.Finite-element analysis of the chip’s flow field was conducted using the COMSOL Multiphysics software,and the simulation results show that the liquid within the chip can flow smoothly,ensuring stable flow fields that facilitate the uniform growth of neurons within the microfluidic channels.By employing MEMS technology in combination with nanomaterial modification techniques,the microfluidic microelectrode array chip was fabricated successfully.Primary hippocampal neurons were cultured on the chip,forming a well-defined neural network.Spontaneous electrical activity of the detected neurons was recorded,exhibiting a 23.7%increase in amplitude compared to neuronal discharges detected on an open-field microelectrode array.This study provides a platform for the precise detection and modulation of patterned neuronal growth in vitro,potentially serving as a novel tool in neuroscience research.

关键词： Microﬂuidic Microelectrode array In vitro Neural chip

HL-2M装置内部输运垒湍流输运的GTC模拟研究

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核技术 2024年第5期47卷 14-20页

作者：肖政耀李新霞王森南华大学核科学技术学院衡阳421001 南华大学核物理研究所衡阳421001

HL-2M托卡马克是我国新一代磁约束聚变等离子体装置,目前已经实现了等离子体电流1 MA的高参数运行模式。本文采用回旋动理学GTC(Gyrokinetic Toroidal Code)程序,研究了具有双输运垒结构的先进运行模式下该装置内部输运垒(Internal Tran... 详细信息

HL-2M托卡马克是我国新一代磁约束聚变等离子体装置,目前已经实现了等离子体电流1 MA的高参数运行模式。本文采用回旋动理学GTC(Gyrokinetic Toroidal Code)程序,研究了具有双输运垒结构的先进运行模式下该装置内部输运垒(Internal Transport Barrier,ITB)的湍流输运。研究结果表明:ITB湍流输运呈现两次连续饱和过程,计算获得的平均离子热扩散系数约为首次饱和水平的两倍。对湍流发展的极向谱和线性色散关系的研究表明,第一次饱和湍流输运由波长kθρi~2.15的短波模式主导,而第二次饱和以kθρi~0.49的长波模式主导;同时,体系湍流的能量由短波模式反向级联到长波模式,湍流饱和阶段径向热扩散系数在输运垒位置处呈现“M”形分布。考虑等离子体带状流结构,数值模拟结果表明:该运行模式下带状流对湍流输运饱和水平没有明显的影响。此外,输运垒等离子体温度和密度梯度最大处对应的湍流饱和期的径向离子热扩散系数最低,极大地增强了对等离子体的约束。

关键词：托卡马克 HL-2M装置 GTC湍流模拟微观不稳定性

Betaine affects muscle lipid metabolism via regulating the fatty acid uptake and oxidation in finishing pig

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Journal of Animal Science and Biotechnology 2018年第1期9卷 187-195页

作者： Sisi Li Haichao wang xinxia wang Yizhen wang Jie Feng Key Laboratory of Animal Nutrition & Feed Zhejiang Province College of Animal Science Zhejiang University

Background： Betaine affects fat metabolism in animals, but the specific mechanism is still not clear. The purpose of this study was to investigate possible mechanisms of betaine in altering lipid metabolism in muscle tissue in finishing pigs.Methods： A total of 120 crossbred gilts（Landrace × Yorkshire × Duroc） with an average initial body weight of 70.1 kg were randomly allotted to three dietary treatments. The treatments included a corn–soybean meal basal diet supplemented with 0, 1250 or 2500 mg/kg betaine. The feeding experiment lasted 42 d.Results： Betaine addition to the diet significantly increased the concentration of free fatty acids（FFA） in muscle（P 〈 0.05）. Furthermore, the levels of serum cholesterol and high-density lipoprotein cholesterol were decreased（P 〈 0.05） and total cholesterol content was increased in muscle（P 〈 0.05） of betaine fed pigs. Experiments on genes involved in fatty acid transport showed that betaine increased expression of lipoprotein lipase（LPL）, fatty acid translocase/cluster of differentiation（FAT/CD36）, fatty acid binding protein（FABP3） and fatty acid transport protein（FATP1）（P 〈 0.05）. The abundance of fatty acid transport protein and fatty acid binding protein were also increased by betaine（P 〈 0.05）. As for the key factors involved in fatty acid oxidation, although betaine supplementation didn＇t affect the level of carnitine and malonyl-CoA, betaine increased mR NA and protein abundance of carnitine palmitransferase-1（CPT1）and phosphorylated-AMPK（P 〈 0.05）.Conclusions： The results suggested that betaine may promoted muscle fatty acid uptake via up-regulating the genes related to fatty acid transporter including FAT/CD36, FATP1 and FABP3. On the other hand, betaine activated AMPK and up-regulated genes related to fatty acid oxidation including PPARα and CPT1. The underlying mechanism regulating fatty acid metabolism in pigs supplemented with betaine is associated with the up-regulation of genes involved in fatty acid transport and fatty acid oxidation.

关键词： Betaine Fatty acid intake Fatty acid oxidation Muscle Pig

Bacillus amyloliquefaciens 40 regulates piglet performance,antioxidant capacity,immune status and gut microbiota

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Animal Nutrition 2023年第1期12卷 116-127页

作者： Zipeng Jiang Weifa Su Wentao Li Chaoyue Wen Shuai Du Huan He Yu Zhang Tao Gong xinxia wang Yizhen wang Mingliang Jin Zeqing Lu National Engineering Research Centre for Green Feed and Healthy Farming Zhejiang UniversityHangzhouChina Key Laboratory of Molecular Nutrition Ministry of EducationZhejiang UniversityHangzhouChina Key Laboratory of Animal Nutrition and Feed Ministry of Agriculture and Rural AffairsZhejiang UniversityHangzhouChina Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province Zhejiang UniversityHangzhouChina College of Animal Science Institute of Feed ScienceZhejiang UniversityHangzhouChina

Probiotics can improve animal growth performance and intestinal health.Bacillus species,Lactobacillus species,Bifidobacterium species,yeast etc.are the common types of probiotics.However,understanding the effects of probiotics on the immune status and gut microbiota of weaning piglets and how the probiotics exert their impact are still limited.This study aimed to investigate the effects of Bacillus amyloliquefaciens 40(BA40)on the performance,immune status and gut microbiota of piglets.A total of 12 litters of newborn piglets were randomly divided into 3 groups.Piglets in control group were orally dosed with phosphate buffered saline;BA40 group and probiotics group were orally gavaged with resuspension BA40 and a probiotics product,respectively.The results showed that BA40 treatment significantly decreased(P<0.05)the diarrhea incidence(from d 5 to 40),diamine oxidase,D-lactate,interleukin(IL)-1βand interferon-γconcentrations compared with control group and probiotics group.Meanwhile BA40 dramatically increased the total antioxidant capacity,IL-10 and secretory immunoglobulin-A concentrations in contrast to control group.For the microbial composition,BA40 modulated the microbiota by improving the abundance of Bacteroides,Phascolarctobacterium(producing short-chain fatty acids)and Desulfovibrio and reducing the proliferation of pathogens(Streptococcus,Tyzzerella,Vellionella and paraeggerthella).Meanwhile,a metabolic function prediction explained that carbohydrate metabolism and amino acid metabolism enriched in BA40 group in contrast to control group and probiotics group.For correlation analysis,the results demonstrated that BA40-enriched Phascolarctobacterium and Desulfovibrio provide insights into strategies for elevating the health status and performance of weaned piglets.Altogether,BA40 exerted stronger ability in decreasing diarrhea incidence and improved antioxidant activity,gut barrier function and immune status of piglets than the other treatments.Our study provided the experimental and theoretical basis fo

关键词： Bacillus amyloliquefaciens Piglet Immune status Gut microbiota Metabolic function

Selective electrochemical production of hydrogen peroxide at zigzag edges of exfoliated molybdenum telluride nanoflakes

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National Science Review 2020年第8期7卷 1360-1366页

作者： Xuan Zhao Yu wang Yunli Da xinxia wang Tingting wang Mingquan Xu Xiaoyun He Wu Zhou Yafei Li Jonathan N.Coleman Yanguang Li Institute of Functional Nano and Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices Soochow University College of Chemistry and Materials Science Nanjing Normal University College of Material Science and Opto-Electronic Technology University of Chinese Academy of Sciences School of Physical Sciences and CAS Key Laboratory of Vacuum Sciences University of Chinese Academy of Sciences School of Physics CRANN and AMBER Centers Trinity College Dublin

The two-electron reduction of molecular oxygen represents an effective strategy to enable the green, mild and on-demand synthesis of hydrogen peroxide. Its practical viability, however, hinges on the development of advanced electrocatalysts, preferably composed of non-precious elements, to selectively expedite this reaction, particularly in acidic medium. Our study here introduces 2H-MoTe;for the first time as the efficient non-precious-metal-based electrocatalyst for the electrochemical production of hydrogen peroxide in acids. We show that exfoliated 2H-MoTe;nanoflakes have high activity(onset overpotential ～140 mV and large mass activity of 27 A g;at 0.4 V versus reversible hydrogen electrode), great selectivity（H;O;percentage up to 93%） and decent stability in 0.5 M H;SO;. Theoretical simulations evidence that the high activity and selectivity of 2H-MoTe;arise from the proper binding energies of HOO;and O;at its zigzag edges that jointly favor the two-electron reduction instead of the four-electron reduction of molecular oxygen.

关键词： hydrogen peroxide production non-precious-metal-based electrocatalyst molybdenum telluride liquid phase exfoliation zigzag edges

Iron polyphthalocyanine sheathed multiwalled carbon nanotubes： A high-performance electrocatalyst for oxygen reduction reaction

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Nano Research 2016年第5期9卷 1497-1506页

作者： xinxia wang Bin wang Jun Zhong Feipeng Zhao Na Han Wenjing Huang Min Zeng Jian Fan Yanguang Li Institute of Functional Nano & Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials and DevicesSoochow University Suzhou 215123 China

The past decade has witnessed a rapid surge of interest in the research and development of non-precious metal-based electrocatalysts for the oxygen reduction reaction （ORR）. Until now, the best catalysts in acidic electrolytes have exclusively been Fe-N-C-type materials from high-temperature pyrolysis. Despite the ORR activities of metal phthalocyanine or porphyrin macrocydes having long been known, their durability remains poor. In this work, we use these macrocycles as a basis to develop a novel organic-carbon hybrid material from in-situ polymerization of iron phthalocyanine on conductive multiwalled carbon nanotube scaffolds using a low-temperature microwave heating method. At an optimal polymer- to-carbon ratio, the hybrid electrocatalyst exhibits excellent ORR activity with a positive half-wave potential （0.80 V）, large mass activity （up to 18.0 A/g at 0.80 V）, and a low peroxide yield （〈3%）. In addition, strong electronic coupling between the polymer and carbon nanotubes is believed to suppress demetallization of the macrocycles, significantly improving cycling stability in acids. Our study represents a rare example of non-precious metal-based electrocatalysts prepared without high-temperature pyrolysis, while having ORR activity in acidic media with potential for practical applications.

关键词： iron polyphthalocyanine,carbon nanotubes,organic-carbon hybrid,oxygen reduction reaction,electrocatalysis

基于物元可拓组合模型的高速公路道路安全风险评价

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重庆交通大学学报（自然科学版） 2024年第4期43卷 37-44页

作者：周洪文冯鑫淼王宏陈昕霞上海应用技术大学城市建设与安全工程学院上海201418 河南省公路工程局集团有限公司河南郑州450000

为准确判定运营期高速公路道路安全水平,找出影响道路安全的关键因素,笔者提出DEMATEL-ISM和物元可拓的组合评价模型。首先,基于道路因素构建初始指标体系,将德尔菲法和敏感性分析相结合来筛选指标,建立高速公路道路安全综合指标体系。... 详细信息

为准确判定运营期高速公路道路安全水平,找出影响道路安全的关键因素,笔者提出DEMATEL-ISM和物元可拓的组合评价模型。首先,基于道路因素构建初始指标体系,将德尔菲法和敏感性分析相结合来筛选指标,建立高速公路道路安全综合指标体系。采用DEMATEL-ISM法分析各指标的重要程度,构建多级层次结构模型,从而了解各指标间的内部影响程度,识别关键影响因素及计算出指标的初始权重;再与熵权法所求的客观权重进行组合优化赋权。最后通过物元可拓模型进行关联度计算,得出各指标及整体的安全风险等级。结果表明:平曲线半径、雨雪等恶劣天气、车速、交通饱和度及纵坡度是影响高速公路道路安全的关键因素;利用模型对河南省某高速的3个不同路段进行实例分析,得出路段1与路段3安全性较高,路段2安全性一般,其评价计算结果符合实际,说明所构建的数学模型准确可行。

关键词：道路工程道路安全物元可拓模型 DEMATEL-ISM 熵权法

饲粮中添加姜黄素和二十二碳六烯酸对育肥猪生长性能、胴体性状、肉品质及血清生化、抗氧化和免疫指标的影响

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动物营养学报 2024年第3期36卷 1537-1548页

作者：陈雨诗刘禹熙杨童寓丹王新霞浙江大学动物科学学院浙江大学动物分子营养教育部重点实验室农业农村部(华东)动物营养与饲料重点实验室浙江省饲料与动物营养重点实验室杭州310058

本试验旨在研究饲粮中添加姜黄素和二十二碳六烯酸(DHA)对育肥猪生长性能、胴体性状、肉品质及血清生化、抗氧化和免疫指标的影响。选取日龄相近、平均体重为(90.76±0.45)kg的“杜×长×大”三元杂交猪54头,随机分为2组,每组3个重复,... 详细信息

本试验旨在研究饲粮中添加姜黄素和二十二碳六烯酸(DHA)对育肥猪生长性能、胴体性状、肉品质及血清生化、抗氧化和免疫指标的影响。选取日龄相近、平均体重为(90.76±0.45)kg的“杜×长×大”三元杂交猪54头,随机分为2组,每组3个重复,每个重复9头猪。对照组饲喂基础饲粮,试验组在基础饲粮中添加0.03%的姜黄素和2.5%的DHA。预试期7 d,正试期40 d。结果表明:1)与对照组相比,试验组的终末体重和平均日增重显著增加(P<0.05),平均日采食量和料重比显著降低(P<0.05)。2)与对照组相比,试验组的背膘厚显著降低(P<0.05),眼肌面积显著增加(P<0.05);肌肉滴水损失显著降低(P<0.05),肌内脂肪含量显著增加(P<0.05)。3)与对照组相比,试验组的背最长肌甘氨酸、脯氨酸、棕榈油酸(C16∶1)和硬脂酸(C18∶0)含量显著增加(P<0.05),肌球蛋白重链Ⅱa(MyHCⅡa)、肌球蛋白重链Ⅱx(MyHCⅡx)和固醇调控元件结合蛋白1(SREBP1)的mRNA相对表达水平显著升高(P<0.05)。4)与对照组相比,试验组的血清甘油三酯和总胆固醇含量显著下降(P<0.05);血清谷胱甘肽过氧化物酶、超氧化物歧化酶活性和总抗氧化能力显著增加(P<0.05),血清丙二醛含量显著降低(P<0.05);血清免疫球蛋白G、免疫球蛋白M和白细胞介素-4含量显著增加(P<0.05),血清谷丙转氨酶和谷草转氨酶活性显著降低(P<0.05)。由此可见,饲粮中添加姜黄素和DHA能提高育肥期“杜×长×大”三元杂交猪的饲料利用率,改善肉品质,并提升血清脂质代谢、抗氧化及免疫能力。

关键词：姜黄素 DHA 育肥猪