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Phenethyl isothiocyanate as an anti-nutritional factor attenuates deoxynivalenol-induced IPEC-J2 cell injury through inhibiting ROSmediated autophagy

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Animal Nutrition 2022年第1期8卷 300-309页

作者： Shuiping Liu xinru mao Lei Ge Lili Hou Guannan Le Fang Gan Lixin Wen Kehe Huang College of Veterinary Medicine Nanjing Agricultural UniversityNanjing210095China Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls Nanjing Agricultural UniversityNanjing210095China MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary MedicineNanjing Agricultural UniversityNanjing210095China College of Veterinary Medicine Hunan Agricultural UniversityChangsha410128China

Deoxynivalenol(DON)is considered to be the most harmful mycotoxin that affects the intestinal health of animals and humans.Phenethyl isothiocyanate(PEITC)in feedstuff is an anti-nutritional factor and impairs nutrient digestion and absorption in the animal intestinal.In the current study,we aimed to explore the effects of PEITC on DON-induced apoptosis,intestinal tight junction disorder,and its potential molecular mechanism in the porcine jejunum epithelial cell line(IPEC-J2).Our results indicated that PEITC treatment markedly alleviated DON-induced cytotoxicity,decreasing the apoptotic cell percentage and pro-apoptotic mRNA/protein levels,and increasing zonula occludens-1(ZO-1),occludin and claudin-1 mRNA/protein expression.Meanwhile,PEITC treatment ameliorated DON-induced an increase of the inducible nitric oxide synthase(iNOS)and cyclooxygenase 2(COX-2)mRNA levels and intracellular reactive oxygen species(ROS)level,and a decrease of glutathione peroxidase 1(GPx1),superoxide dismutase 2(SOD2),catalase(CAT)and heme oxygenase 1(HO-1)mRNA levels.Additionally,PEITC treatment significantly down-regulated autophagy-related protein 5(ATG5),beclin-1 and microtubuleassociated protein 1 light chain 3B(LC3-II)mRNA/protein levels,decreased the number of green fluorescent protein-microtubule-associated protein 1 light-chain 3(GFP-LC3)puncta and phosphatidylinositol 3 kinase(PI3K)protein expression,and up-regulated phospho-protein kinase B(p-Akt)and phospho-mammalian target of rapamycin(p-mTOR)protein expression against DON.However,the activation of autophagy by rapamycin,an autophagy agonist,abolished the protective effects of PEITC against DON-induced cytotoxicity,apoptosis and intestinal tight junction disorder.Collectively,PEITC could confer protection against DON-induced porcine intestinal epithelial cell injury by suppressing ROSmediated autophagy.

关键词： Deoxynivalenol Phenethyl isothiocyanate Autophagy Oxidative damage Porcine intestinal epithelial cell

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Highly Selective Electrocatalytic CuEDTA Reduction by MoS_(2) Nanosheets for Efficient Pollutant Removal and Simultaneous Electric Power Output

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纳微快报:英文版 2023年第11期15卷 61-73页

作者： Hehe Qin xinru Liu Xiangyun Liu Hongying Zhao Shun mao College of Environmental Science and Engineering Biomedical Multidisciplinary Innovation Research InstituteShanghai East HospitalState Key Laboratory of Pollution Control and Resource ReuseTongji University1239 Siping RoadShanghai 200092People’s Republic of China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092People’s Republic of China Shanghai Key Lab of Chemical Assessment and Sustainability School of Chemical Science and EngineeringTongji University1239 Siping RoadShanghai 200092People’s Republic of China

Electrocatalytic reduction of ethylenediamine tetraacetic acid copper(CuEDTA),a typical refractory heavy metal complexation pollutant,is an environmental benign method that operates at mild condition.Unfortunately,the selective reduction of CuEDTA is still a big challenge in cathodic process.In this work,we report a MoS_(2) nanosheet/graphite felt(GF)cathode,which achieves an average Faraday efficiency of 29.6%and specific removal rate(SRR)of 0.042 mol/cm^(2)/h for CuEDTA at−0.65 V vs SCE(saturated calomel electrode),both of which are much higher than those of the commonly reported electrooxidation technology-based removal systems.Moreover,a proofof-concept CuEDTA/Zn battery with Zn anode and MoS_(2)/GF cathode is demonstrated,which has bifunctions of simultaneous CuEDTA removal and energy output.This is one of the pioneer studies on the electrocatalytic reduction of heavy metal complex and CuEDTA/Zn battery,which brings new insights in developing efficient electrocatalytic reduction system for pollution control and energy output.

关键词： Electrocatalytic reduction CuEDTA removal MoS_(2)nanosheet CuEDTA/Zn battery Faraday efficiency

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Immune‑Ageing Evaluation of Peripheral T and NK Lymphocyte Subsets in Chinese Healthy Adults

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Phenomics 2023年第4期3卷 360-374页

作者： Zhenghu Jia Zhiyao Ren Dongmei Ye Jiawei Li Yan Xu Hui Liu Ziyu Meng Chengmao Yang Xiaqi Chen xinru mao Xueli Luo Zhe Yang Lina Ma Anyi Deng Yafang Li Bingyu Han Junping Wei Chongcheng Huang Zheng Xiang Guobing Chen Peiling Li Juan Ouyang Peisong Chen Oscar Junhong Luo Yifang Gao Zhinan Yin Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment Zhuhai Institute of Translational Medicine Zhuhai People’s Hospital Affiliated With Jinan UniversityJinan UniversityZhuhai 519000GuangdongChina The Biomedical Translational Research Institute Health Science Center(School of Medicine)Jinan UniversityGuangzhou 510632GuangdongChina Guangzhou Purui Biotechnology Co. Ltd.Guangzhou 510660GuangdongChina Department of Systems Biomedical Sciences School of MedicineJinan UniversityGuangzhou 510632GuangdongChina Guangzhou Geriatric Hospital Guangzhou 510550GuangdongChina Organ Transplantation Center The First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080GuangdongChina Emergency Department The First Affiliated Hospital of Jinan UniversityJinan UniversityGuangzhou 510632GuangdongChina NHC Key Laboratory of Hormones and Development Tianjin Key Laboratory of Metabolic DiseasesChu Hsien-I Memorial HospitalTianjin Medical UniversityTianjin 300134China Tianjin Institute of Endocrinology Tianjin Medical UniversityTianjin 300134China Zhongke Regenerative Medicine Technology Co. LtdDongguan 523808GuangdongChina Wuhan Purui Medical Laboratory Co. LtdWuhan 430223HubeiChina Department of Clinical Laboratory Department of Laboratory MedicineThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080GuangdongChina

Ageing is often accompanied with a decline in immune system function,resulting in immune ageing.Numerous studies have focussed on the changes in different lymphocyte subsets in diseases and immunosenescence.The change in immune phenotype is a key indication of the diseased or healthy status.However,the changes in lymphocyte number and phenotype brought about by ageing have not been comprehensively analysed.Here,we analysed T and natural killer(NK)cell subsets,the phenotype and cell differentiation states in 43,096 healthy individuals,aged 20–88 years,without known diseases.Thirty-six immune parameters were analysed and the reference ranges of these subsets were established in different age groups divided into 5-year intervals.The data were subjected to random forest machine learning for immune-ageing modelling and confirmed using the neural network analysis.Our initial analysis and machine modelling prediction showed that na.ve T cells decreased with ageing,whereas central memory T cells(Tcm)and effector memory T cells(Tem)increased cluster of differentiation(CD)28-associated T cells.This is the largest study to investigate the correlation between age and immune cell function in a Chinese population,and provides insightful differences,suggesting that healthy adults might be considerably influenced by age and sex.The age of a person's immune system might be different from their chronological age.Our immune-ageing modelling study is one of the largest studies to provide insights into‘immune-age’rather than‘biological-age’.Through machine learning,we identified immune factors influencing the most through ageing and built a model for immune-ageing prediction.Our research not only reveals the impact of age on immune parameter differences within the Chinese population,but also provides new insights for monitoring and preventing some diseases in clinical practice.

关键词： Immunosenescence Phenotype T cell subset Natural killer cell subsets Immune-ageing modelling

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MicroRNA-21/PARP-1作为生物标志物在AR和CARAS中的诊断价值分析

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南京医科大学学报（自然科学版） 2024年第6期44卷 860-867页

作者：肖心儒丁紫琪刘志光毛正道张倩南京医科大学附属常州市第二人民医院呼吸与危重症医学科南京医科大学常州医学中心江苏常州213003

目的:评估外周血microRNA(miR)-21、血浆聚腺苷二磷酸核糖聚合酶1[poly(ADP-ribose)polymerase-1,PARP-1]在过敏性鼻炎(allergic rhinitis,AR)和过敏性鼻炎-哮喘综合征(combined allergic rhinitis and asthma syndrome,CARAS)中的诊断价值。方法:收集44例CARAS患者、31例AR患者和42例健康对照的外周血,采用RT-qPCR法检测外周血中miR-21的表达水平,采用ELISA法检测血浆中PARP-1蛋白水平。应用Pearson进行相关性分析。受试者工作特征(receiver operating characteristic,ROC)曲线判断miR-21和PARP-1的诊断灵敏度与特异度。结果:CARAS组患者外周血miR-21的表达较健康对照组升高。AR组患者血浆PARP-1的水平较CARAS组和健康对照组升高。Pearson相关性分析结果显示,外周血miR-21的表达水平在AR患者中与嗜酸性粒细胞计数相关,在CARAS患者中与鼻呼出气一氧化氮(fractional nasal nitric oxide,FnNO)水平相关;血浆PARP-1在AR患者中与1秒钟用力呼气量占预计值百分比(forced expiratory volume in onesecond percent predicted,FEV_(1)%_(pred))相关,在CARAS患者中与FEV_(1)%_(pred)及1秒钟用力呼气量(forced expiratory volume in one second,FEV_(1))/用力肺活量(forced vital capacity,FVC)(FEV_(1)/FVC)相关。ROC曲线分析显示,外周血miR-21作为CARAS的诊断标志物时,灵敏度为51.35%,特异度为80.95%。血浆PARP-1作为AR的诊断标志物时,灵敏度为90.32%,特异度为54.76%。血浆PARP-1作为AR进展为CARAS的诊断标志物时,灵敏度为45.45%,特异度为90.32%。结论:AR和CARAS患者外周血miR-21、PARP-1存在差异表达,外周血miR-21可作为CARAS的诊断标志物,PARP-1可作为AR的诊断标志物及AR进展为CARAS的生物标志物。这对寻求AR和CARAS的诊治靶点有十分重要的价值。

关键词：过敏性鼻炎-哮喘综合征 microRNA-21 PARP-1 生物标志物

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Prevalence and clinical significance of pathogenic germline BRCA1/2 mutations in Chinese non-small cell lung cancer patients

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Cancer Biology & Medicine 2019年第3期16卷 556-564页

作者： Xingsheng Hu Dongyong Yang Yalun Li Li Li Yan Wang Peng Chen Song Xu Xingxiang Pu Wei Zhu Pengbo Deng Junyi Ye Hanhan Zhang Analyn Lizaso Hao Liu xinru mao Hai Huang Qian Chu Chengping Hu Department of Medical Oncology Cancer HospitalChinese Academy of Medical SciencesBeijing100021China Department of Pulmonary Medicine Second Affiliated Hospital of Fujian Medical UniversityQuanzhou362100China Department of Respiratory Medicine West China HospitalSichuan UniversityChengdu610041China Department ofRespiratory Medicine Daping HospitalChongqing400042China Department of Respiratory Medicine Xinqiao HospitalArmy Medical UniversityChongqing400037China Department of Medical Oncology Tianjin Medical University CancerInstitute and HospitalNational Clinical Research Center for Cancer Key Laboratory of Cancer Prevention and Therapy Tianjin Tianjin's Clinical Research Center for Cancer Tianjin300060China Department of Thoracic Oncology TianjinMedical University General HospitalTianjin300052China Department of Medical Oncology Hunan Cancer HospitalChangsha410006China Department of Pathology Xiangya HospitalCentral South UniversityChangsha410008China Department of Respiratory Medicine Xiangya HospitalCentral South UniversityChangsha410008China Burning RockBiotech Guangzhou510300China Department of Oncology Tongji Hospital of Tongji Medical CollegeHuazhongUniversity of Science and TechnologyWuhan430022China

Objective: Germline alterations in the breast cancer susceptibility genes type 1 and 2, BRCA1 and BRCA2, predispose individuals to hereditary cancers, including breast, ovarian, prostate, pancreatic, and stomach cancers.Accumulating evidence suggests inherited genetic susceptibility to lung cancer.The present study aimed to survey the prevalence of pathogenic germline BRCA mutations(gBRCAm) and explore the potential association between gBRCAm and disease onset in Chinese advanced non-small cell lung cancer(NSCLC) patients.Methods: A total of 6,220 NSCLC patients were screened using capture-based ultra-deep targeted sequencing to identify patients harboring germline BRCA1/2 mutations.Results: Out of the 6,220 patients screened, 1.03%(64/6,220) of the patients harbored the pathogenic gB RCAm, with BRCA2 mutations being the most predominant mutations(49/64, 76.5%).Patients who developed NSCLC before 50 years of age were more likely to carry gBRCAm(P = 0.036).Among the patients harboring classic lung cancer driver mutations, those with concurrent gBRCAm were significantly younger than those harboring the wild-type gBRCA(P = 0.029).By contrast, the age of patients with or without concurrent gBRCAm was comparable to those of patients without the driver mutations(P = 0.972).In addition, we identified EGFR-mutant patients with concurrent gBRCAm who showed comparable progression-free survival but significantly longer overall survival(P = 0.002) compared to EGFR-mutant patients with wild-type germline BRCA.Conclusions: Overall, our study is the largest survey of the prevalence of pathogenic gBRCAm in advanced Chinese NSCLC patients.Results suggested a lack of association between germline BRCA status and treatment outcome of EGFR-TKI.In addition,results showed a positive correlation between pathogenic gB RCAm and an early onset of NSCLC.

关键词： Germline BRCA mutations non-small cell lung cancer prevalence BRCA1 BRCA2

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聚二甲基硅氧烷膜蒸汽渗透分离乙醇/二氧化碳的研究

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膜科学与技术 2024年第2期44卷 29-36页

作者：石新宇谢纹纹毛恒王涛陈欣如齐昊胡晓雪赵之平北京理工大学化学与化工学院北京102488

生物质发酵制乙醇过程中,发酵尾气的产生与排放对生态环境有极大影响.本文以聚偏氟乙烯(PVDF)多孔膜为基底,聚二甲基硅氧烷(PDMS)为分离层基质,制备出适用于发酵尾气乙醇/CO_(2)分离的PDMS/PVDF复合膜.通过红外光谱、X射线衍射、扫描电... 详细信息

生物质发酵制乙醇过程中,发酵尾气的产生与排放对生态环境有极大影响.本文以聚偏氟乙烯(PVDF)多孔膜为基底,聚二甲基硅氧烷(PDMS)为分离层基质,制备出适用于发酵尾气乙醇/CO_(2)分离的PDMS/PVDF复合膜.通过红外光谱、X射线衍射、扫描电子显微镜等手段对PDMS/PVDF复合膜进行了系统表征,探究了热交联时间、进料压力、进料乙醇浓度、进料温度、吹扫气流量对PDMS/PVDF复合膜渗透分离性能的影响.结果表明,在PVDF基底上成功制备出薄约10.5μm、致密且均质的PDMS分离层.热交联时间越长,膜的选择性越低而渗透性升高;随着进料压力增加,膜的渗透性和选择性均升高;随着进料乙醇浓度升高,膜的渗透性和选择性均呈先升后降的趋势;随着进料温度升高,膜的渗透性和选择性均下降;随吹扫气流量的增加,膜的渗透性和选择性先升高后下降.在进料温度35℃、进料压力0.15 MPa(表压)、乙醇蒸汽体积分数0.1%时,所制PDMS/PVDF复合膜的乙醇渗透性高达33052.2 Barrer,乙醇/CO_(2)选择性为17.1.此外,在连续测试60 h中,所制复合膜性能保持稳定,在生物乙醇发酵尾气处理方面具有应用前景.

关键词：蒸汽渗透聚二甲基硅氧烷复合膜发酵尾气乙醇回收

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锂/钠-氯二次电池的最新进展——从材料构建到性能评估

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储能科学与技术 2024年第6期13卷 1824-1834页

作者：杨建航冯文婷韩俊伟魏欣茹马晨宇毛常明智林杰孔德斌青岛科技大学材料科学与工程学院山东青岛266042 中国石油大学(华东)高端化工与能源材料研究中心中国石油大学(华东)新能源学院山东青岛266580

传统锂离子电池的能量密度已难以满足日益增长的更高能量密度的需求。开发新型高能量密度二次电池是最为有效的一个策略。近期,基于商用一次锂-亚硫酰氯锂电池衍生而来的锂/钠-氯二次电池因其高能量密度而备受关注,成为替代传统锂离子... 详细信息

传统锂离子电池的能量密度已难以满足日益增长的更高能量密度的需求。开发新型高能量密度二次电池是最为有效的一个策略。近期,基于商用一次锂-亚硫酰氯锂电池衍生而来的锂/钠-氯二次电池因其高能量密度而备受关注,成为替代传统锂离子电池的有力竞争者。本文围绕锂/钠-氯二次电池的最新研究进展,综述了正极载体、负极及电解液等关键组分构建研究及其对电化学性能的影响。在正极载体方面,系统阐述了碳材料、共轭框架聚合物等载体设计对锂/钠-氯二次电池首次放电容量、可逆容量、倍率性能和温度的影响;在电解液方面,详细分析了针对反应机理、中间相产物和电解液腐蚀问题的解决策略;并简要介绍了适用于锂/钠-氯二次电池的新型合金负极。基于正极载体的理性设计与电解液系统优化,锂/钠-氯二次电池在新型二次电池领域已初现峥嵘,循环寿命可达500圈,尤其是在极端服役环境中表现优异(可在-80℃工作,电流密度最大可达16 A/g)。然而,氯物种转化动力学速率慢、活性氯物种利用率低以及氯物种对负极等的腐蚀难题仍然是限制其性能进一步提升的瓶颈,也是未来亟待解决的挑战所在。

关键词：锂/钠-氯二次电池高能量密度电极材料设计结构性能关系电化学反应动力学

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5例16p13.11微重复胎儿的产前诊断及遗传学分析

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中国计划生育和妇产科 2024年第1期16卷 39-42,I0003页

作者：陈敏晖黄秀琼刘欣茹林雨虹毛雅珍福建医科大学附属福州市第一医院检验科福建福州350009 福建医科大学附属福州市第一医院产科福建福州350009

目的针对5例16p13.11区出现拷贝数变异(copy number variants,CNVs)的胎儿进行产前诊断及遗传学分析。方法对2019年9月至2022年12月在福建医科大学附属福州市第一医院5例因不同产前诊断指征的胎儿羊水进行常规染色体G显带和单核苷酸多... 详细信息

目的针对5例16p13.11区出现拷贝数变异(copy number variants,CNVs)的胎儿进行产前诊断及遗传学分析。方法对2019年9月至2022年12月在福建医科大学附属福州市第一医院5例因不同产前诊断指征的胎儿羊水进行常规染色体G显带和单核苷酸多态性微阵列(single nucleotide polymorphism array,SNP array)检测,对诊断为16p13.11微重复的胎儿遗传学进行回顾性分析。结果5例胎儿染色体核型分析结果未见异常;SNP array结果显示在16p13.11区域存在着846.1~1600 kb的微重复,共涉及到NDE1、ABCC6、MYH11等6个OMIM基因;其中2例未进行遗传溯源,另3例遗传自父源或母源的16p13.11微重复片段的胎儿足月分娩,随访未见异常。结论16p13.11片段微重复胎儿产前诊断存在表型不完全外显,且缺乏特异性的表型。

关键词： 16p13.11 拷贝数变异 SNP array 产前诊断

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红叶石楠叶斑病致病菌的分离与鉴定

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生物灾害科学 2024年第1期47卷 34-41页

作者：欧阳心如李栋良张诺涵茅建翔宋蕴哲蔡普默武夷学院茶与食品学院福建武夷山354300 福建农林大学资源与环境学院福建福州350007

【目的】为明确红叶石楠患叶斑病叶片的致病菌,以期为红叶石楠叶斑病的防控提供借鉴参考。【方法】2022年9月以福建省南平市武夷山市武夷学院采集的患病叶片为材料,利用传统微生物培养法分离纯化致病菌,进行柯赫氏法则验证,通过形态学... 详细信息

【目的】为明确红叶石楠患叶斑病叶片的致病菌,以期为红叶石楠叶斑病的防控提供借鉴参考。【方法】2022年9月以福建省南平市武夷山市武夷学院采集的患病叶片为材料,利用传统微生物培养法分离纯化致病菌,进行柯赫氏法则验证,通过形态学鉴定和ITS序列分析明确致病菌的种类。【结果】从病叶中分离得到的菌株a在PDA培养基上呈白色絮状,有明显的轮纹,分生孢子为纺锤形,菌株a的ITS序列与NCBI数据库中小孢拟盘多毛孢属(Pestalotiopsis microspora,登录号:AY924271.1)的相似性为99%。菌株b在PDA培养基上呈白色蓬松状,有大量气生菌丝,孢子呈黑色球状,菌株b的ITS序列与NCBI数据库中稻黑孢属(Nigrospora oryzae,登录号:KT192348.1)的相似性为99%。接种菌株a和菌株b均能使离体叶片发病,且症状与供试叶片的症状相似。【结论】引发武夷学院红叶石楠叶斑病的病原物为小孢拟盘多毛孢菌和稻黑孢菌。

关键词：红叶石楠致病菌形态鉴定分子鉴定系统发育树

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河北省廊坊市牛主要病毒性腹泻病原感染状况检测及牛冠状病毒演化分析

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畜牧兽医学报 2024年第2期55卷 649-659页

作者：李思远付新成袁雪松毛立蔡旭航孙心如黄金谢玲玲王府周华张琪李基棕李彬西北农林科技大学动物医学院杨凌712100 江苏省农业科学院兽医研究所农业农村部兽用生物制品工程技术重点实验室南京210014 南京农业大学动物医学院南京210095 江苏大学生命科学学院食品与生物工程学院镇江212013 河北省廊坊市农业农村局廊坊065000 贵州省种畜禽种质测定中心贵阳550018 黔西市动物疫病预防控制中心黔西551500

牛病毒性腹泻病原严重危害畜牧业生产,BVDV、BoAstV、BCoV、MRV、BKoV、BoNeV、BNoV、BRV、BToV被认为是引起牛群腹泻的病原,不仅如此,BVDV、BCoV和BToV除了能引起消化道症状外还能引起呼吸道症状,BVDV不仅能引起消化道和呼吸道症状,还... 详细信息

牛病毒性腹泻病原严重危害畜牧业生产,BVDV、BoAstV、BCoV、MRV、BKoV、BoNeV、BNoV、BRV、BToV被认为是引起牛群腹泻的病原,不仅如此,BVDV、BCoV和BToV除了能引起消化道症状外还能引起呼吸道症状,BVDV不仅能引起消化道和呼吸道症状,还会导致母牛生殖功能异常,这些疾病对畜牧业造成巨大的经济损失。为了解牛主要病毒性腹泻病原流行情况,本次试验通过PCR检测方法对我国河北地区323份腹泻牛粪样品进行常见9种病毒性牛腹泻病原进行检测。结果显示,BVDV阳性检出率1.85%(6/323)、BCoV阳性检出率14.24%(46/323)、BRV阳性检出率57.89%(187/323)、BoAstV阳性检出率0.31%(1/323)、BoNeV阳性检出率10.84%(35/323)、BNoV阳性检出率4.33%(14/323)、MRV阳性检出率2.48%(8/323)、BToV阳性检出率4.64%(15/323)、BKoV阳性检出率11.76%(38/323)。混合感染共有76份,占比23.53%(76/323)。HBLF2302株的全基因序列、S、HE、N、M和E基因进行同源性和遗传进化分析发现,HBLF2302与BCoV/NMG1/2022基因同源性最高,为GIIb亚型。基于氨基酸序列比对和HBLF2302的S蛋白三级结构预测发现,在S蛋白中157号位突变为157T,854号位点突变为854I。318V为独特突变,改变了与侧链残基的作用力,与631C形成氢键连接。并发现中国株区别于其它地区的GⅡb亚型,位于ORF1a区域有三个独特位点。本研究丰富了牛腹泻病原的流行病学数据,为牛腹泻病的防控奠定基础。

关键词：病毒性腹泻牛冠状病毒流行病学调查腹泻病原

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维普期刊数据库

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