检索结果-南通市图书馆

基于全基因组重测序的油莎豆InDel位点鉴定及分子标记开发

在线全文

学校读者我要写书评

暂无评论

种子 2024年第8期43卷 52-59页

作者：张向歌程珊陈晨朱雅婧李春鑫许欣然王树峰武林海王会伟河南省农业科学院经济作物研究所郑州450002

选取4种不同类型油莎豆种质资源进行全基因组重测序,以此全基因组水平鉴定油莎豆的InDel位点,并根据多态性InDel位点序列信息开发有效的分子标记。基于重测序数据与油莎豆参考基因组的序列比对,利用GATK和SAMtools软件共鉴定到321271个I... 详细信息

选取4种不同类型油莎豆种质资源进行全基因组重测序,以此全基因组水平鉴定油莎豆的InDel位点,并根据多态性InDel位点序列信息开发有效的分子标记。基于重测序数据与油莎豆参考基因组的序列比对,利用GATK和SAMtools软件共鉴定到321271个InDel位点,在油莎豆各个scaffold上呈现高密度分布,其中93%以上位点呈现出杂合类型。通过4种油莎豆种质间InDel位点的多态性分析,发现98%的InDel位点不具有多态性,最终鉴定出6036个多态性InDel位点。基于多态性InDel位点的插入/缺失序列长度分析,筛选出829个插入/缺失序列长度大于20 bp的多态性InDel位点,适用于开发可通过琼脂糖凝胶电泳检测的InDel分子标记。选择8个多态性位点进行InDel分子标记开发和琼脂糖凝胶电泳检验,结果表明,鉴定的多态性InDel位点真实可靠以及开发的InDel分子标记合适可用。本研究利用不同类型油莎豆种质鉴定出大量的多态性InDel位点,能够开发出适合、准确、有效的InDel分子标记,极大地丰富了油莎豆的分子标记资源,为油莎豆种质资源更加精确的评价、鉴定和利用提供可靠的技术支撑。

关键词：油莎豆种质资源全基因组重测序 InDel位点分子标记

在线全文

学校读者我要写书评

暂无评论

基于容忍因子的近似最近邻混合查询算法

大数据 2024年第1期10卷 17-34页

作者：贺广福薛源海陈翠婷俞晓明刘欣然程学旗中国科学院计算技术研究所北京100190 中国科学院大学北京101408 北京邮电大学北京100876

近似最近邻搜索(ANNS)是计算机领域中一种重要的高效相似度搜索技术,可用于在大规模数据集中进行快速信息检索。随着人们对高精度信息检索的需求不断增长,同时使用结构化信息和非结构化信息进行混合查询的方式也得到了广泛应用。然而,... 详细信息

近似最近邻搜索(ANNS)是计算机领域中一种重要的高效相似度搜索技术,可用于在大规模数据集中进行快速信息检索。随着人们对高精度信息检索的需求不断增长,同时使用结构化信息和非结构化信息进行混合查询的方式也得到了广泛应用。然而,基于近邻图的过滤贪心算法在混合查询时可能会因结构化约束条件的影响导致连通性降低,进而损害搜索精度。为此,提出了一种基于容忍因子的过滤贪心算法,通过容忍因子控制不满足结构化约束条件的顶点参与路由,在不改变索引结构的前提下维持原有近邻图的连通性,克服了结构化约束条件对检索精度的负面影响。实验结果证明,新算法可以在不同结构化约束强度下实现ANNS的高精度搜索,同时保持检索效率。该研究解决了基于近邻图的ANNS在混合查询场景中的问题,为大规模数据集的快速混合查询信息检索提供了一种有效的解决方案。

关键词：混合查询向量检索最近邻搜索过滤搜索

Identification of de novo Mutations in the Chinese Autism Spectrum Disorder Cohort via Whole-Exome Sequencing Unveils Brain Regions Implicated in Autism

在线全文

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2023年第10期39卷 1469-1480页

作者： Bo Yuan Mengdi Wang xinran Wu Peipei cheng Ran Zhang Ran Zhang Shunying Yu Jie Zhang Yasong Du Xiaoqun Wang Zilong Qiu Songjiang Research Institute Songjiang HospitalShanghai Jiao Tong University School of MedicineShanghai 201600China Shanghai Mental Health Center Shanghai Jiao Tong University School of MedicineShanghai 200032China Institute of Neuroscience State Key Laboratory of NeuroscienceCAS Center for Excellence in Brain Science and Intelligence TechnologyShanghai Center for Brain Science and Brain-Inspired Intelligence TechnologyChinese Academy of SciencesShanghai 200032China Beijing Normal University Beijing 100875China Institute of Biophysics Chinese Academy of SciencesBeijing 100101China Institute of Science and Technology for Brain-Inspired Intelligence Fudan UniversityShanghai 200433China

Autism spectrum disorder(ASD)is a highly heritable neurodevelopmental disorder characterized by deficits in social interactions and repetitive *** hundreds of ASD risk genes,implicated in synaptic formation and transcriptional regulation,have been identified through human genetic studies,the East Asian ASD cohorts are still under-represented in genome-wide genetic ***,we applied whole-exome sequencing to 369 ASD trios including probands and unaffected parents of Chinese *** a joint-calling analytical pipeline based on GATK toolkits,we identified numerous de novo mutations including 55 high-impact variants and 165 moderate-impact variants,as well as de novo copy number variations containing known ASD-related ***,combined with single-cell sequencing data from the developing human brain,we found that the expression of genes with de novo mutations was specifically enriched in the pre-,post-central gyrus(PRC,PC)and banks of the superior temporal(BST)regions in the human *** further analyzing the brain imaging data with ASD and healthy controls,we found that the gray volume of the right BST in ASD patients was significantly decreased compared to healthy controls,suggesting the potential structural deficits associated with ***,we found a decrease in the seed-based functional connectivity between BST/PC/PRC and sensory areas,the insula,as well as the frontal lobes in ASD *** work indicated that combinatorial analysis with genome-wide screening,single-cell sequencing,and brain imaging data reveal the brain regions contributing to the etiology of ASD.

关键词： Autism Cohorts Whole-exome sequencing Single-cell sequencing

Single-cell analyses reveal cannabidiol rewires tumor microenvironment via inhibiting alternative activation of macrophage and synergizes with anti-PD-1 in colon cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

药物分析学报:英文版 2023年第7期13卷 726-744页

作者： Xiaofan Sun Lisha Zhou Yi Wang Guoliang Deng xinran Cao Bowen Ke Xiaoqi Wu Yanhong Gu Haibo cheng Qiang Xu Qianming Du Hongqi Chen Yang Sun Department of Rheumatology and Immunology Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing UniversityNanjing210008China State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing210023China Colon and Rectal Surgery Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese MedicineNanjing210001China Laboratory of Anesthesia and Critical Care Medicine Department of AnesthesiologyTranslational Neuroscience CenterWest China Hospital and State Key Laboratory of BiotherapySichuan UniversityChengdu610044China Genergy Bio-technology(Shanghai)Co. LtdShanghai200241China The First Affiliated Hospital of Nanjing Medical University Jiangsu Province HospitalNanjing210029China Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine in Prevention and Treatment of Tumor The First Clinical College of Nanjing University of Chinese MedicineNanjing210023China General Clinical Research Center Nanjing First HospitalNanjing Medical UniversityNanjing210006China School of Basic Medicine&Clinical Pharmacy China Pharmaceutical UniversityNanjing210009China Department of General Surgery Shanghai Jiao Tong University Affiliated Sixth People's HospitalShanghai200030China Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy Xuzhou Medical UniversityXuzhouJiangsu221004China

Colorectal tumors often create an immunosuppressive microenvironment that prevents them from responding to ***(CBD)is a non-psychoactive natural active ingredient from the cannabis plant that has various pharmacological effects,including neuroprotective,antiemetic,anti-inflammatory,and antineoplastic *** study aimed to elucidate the specific anticancer mechanism of CBD by single-cell RNA sequencing(scRNA-seq)and single-cell ATAC sequencing(scATAC-seq)***,we report that CBD inhibits colorectal cancer progression by modulating the suppressive tumor microenvironment(TME).Our single-cell transcriptome and ATAC sequencing results showed that CBD suppressed M2-like macrophages and promoted M1-like macrophages in tumors both in strength and ***,CBD significantly enhanced the interaction between M1-like macrophages and tumor cells and restored the intrinsic anti-tumor properties of macrophages,thereby preventing tumor ***,CBD altered the metabolic pattern of macrophages and related anti-tumor signaling *** found that CBD inhibited the alternative activation of macrophages and shifted the metabolic process from oxidative phosphorylation and fatty acid oxidation to glycolysis by inhibiting the phosphatidylinositol 3-kinase-protein kinase B signaling pathway and related downstream target ***,CBD-mediated macrophage plasticity enhanced the response to anti-programmed cell death protein-1(PD-1)immunotherapy in xenografted *** together,we provide new insights into the anti-tumor effects of CBD.

关键词： scRNA-seq scATAC-seq Cannabidiol Colorectal cancer Tumor microenvironment Macrophage

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

小麦穗发芽抗性鉴定标准的改进与优化

安徽农业大学学报 2021年第2期48卷 179-184页

作者：高威徐康乐刘雪徐冬梅高畅严胜男姚辉尚姚姚潘旭曹佳佳程欣然刘明利卢杰常成张海萍马传喜安徽农业大学农学院农业农村部黄淮南部小麦生物学与遗传育种重点实验室合肥230036

参照农业部小麦穗发芽抗性鉴定行业标准(NY/T1739-2009)对国家小麦良种联合攻关2018—2019年度40份小麦新品系进行穗发芽抗性鉴定。结果发现,黄淮南片广适组和抗赤霉病组等29份半冬性小麦品系穗发芽抗性均为感或高感,未鉴定出抗性品系,... 详细信息

参照农业部小麦穗发芽抗性鉴定行业标准(NY/T1739-2009)对国家小麦良种联合攻关2018—2019年度40份小麦新品系进行穗发芽抗性鉴定。结果发现,黄淮南片广适组和抗赤霉病组等29份半冬性小麦品系穗发芽抗性均为感或高感,未鉴定出抗性品系,且品系间变异系数(11.23%和22.06%)明显低于长江上、中下游区试验组的11份品系(88.09%,45.45%),半冬性品系的穗发芽抗性区分度较低。因此,根据该行业标准,从2019—2020年度128份国家小麦良种联合攻关参试品系中随机选取25份和18份试验材料,分别设置2、3和4 d共3个发芽处理时间,研究不同发芽时间对穗发芽抗性鉴定的影响,并对穗发芽时间进行优化,以确定合适的穗发芽处理时间。结果表明,和穗发芽3、4 d相比(27.44%,13.84%),发芽2 d的品系间穗发芽抗性差异明显(高感—高抗),变异系数较大(46.26%,54.89%)。进一步对128份品系的穗发芽抗性鉴定,结果也发现发芽处理2d,各参试组穗发芽抗性变异较大,能够有效区分半冬性品系间抗性水平,与生产实际相符。

关键词：小麦穗发芽抗性鉴定标准优化

3C疗法治疗1型糖尿病儿童维生素D水平研究

在线全文

学校读者我要写书评

暂无评论

儿科药学杂志 2020年第12期26卷 10-14页

作者：鄢力程昕然龚春竹唐芳苟鹏李中会苏娜冷洁汪柳旭杜思泓张红许珂电子科技大学医学院附属妇女儿童医院成都市妇女儿童中心医院四川成都611731

目的:评估维生素D与1型糖尿病(T1DM)患儿的临床关系,为T1DM的防治提供新的依据。方法:选取我院2017-2018年新诊断及使用3C疗法治疗的T1DM患儿,分析T1DM患儿与健康体检患儿血清25-羟维生素D[25(OH)D]水平。根据25(OH)D水平,将T1DM患儿分... 详细信息

目的:评估维生素D与1型糖尿病(T1DM)患儿的临床关系,为T1DM的防治提供新的依据。方法:选取我院2017-2018年新诊断及使用3C疗法治疗的T1DM患儿,分析T1DM患儿与健康体检患儿血清25-羟维生素D[25(OH)D]水平。根据25(OH)D水平,将T1DM患儿分为三组(缺乏组、不足组及充足组),比较三个亚组的性别、年龄、居住地、体质量指数(BMI)、空腹C肽、空腹血糖及胰岛素用量等情况,探讨不同tanner分期、性别、季节、有无合并糖尿病酮症酸中毒(DKA)与血清维生素D水平的关系。结果:T1DM组患儿血清25(OH)D水平为(42.31±22.01)nmol/L,较健康对照组的(50.37±22.28)nmol/L低,差异有统计学意义(P<0.05)。T1DM组患儿中,维生素D充足组的空腹C肽水平高于维生素D不足组及维生素D缺乏组(P<0.05);维生素D缺乏组空腹血糖水平及单位体质量胰岛素用量高于维生素D充足组(P<0.05)。按血清25(OH)D水平测定时间不同分A组(1-3月、10-12月)和B组(4-9月),A组25(OH)D水平低于B组(P<0.05);合并DKA组25(OH)D水平低于无DKA组(P<0.05)。结论:T1DM患儿普遍存在维生素D缺乏,尤其是合并DKA、1-3月及10-12月的患儿;维生素D充足的T1DM患儿可减少胰岛素用量。临床上要加强T1DM患儿的维生素D的监测以及补充。

关键词：维生素D 1型糖尿病儿童 3C疗法

A subpopulation of CD146^(+) macrophages enhances antitumor immunity by activating the NLRP3 inflammasome

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2023年第8期20卷 908-923页

作者： Lin Jing Yunhe An Tanxi Cai Jianquan Xiang Baoming Li Jiang Guo xinran Ma Ling Wei Yanjie Tian Xiaoyan cheng Xuehui Chen Zheng Liu Jing Feng Fuquan Yang Xiyun Yan Hongxia Duan Key Laboratory of Protein and Peptide Pharmaceutical Institute of BiophysicsChinese Academy of SciencesBejing 100101China Institute of Analysis and Testing Beijing Academy of Science and Technology(Bejing Center for Physical and Chemical Analysis)No.7 Fengxian Middle StreetHaidian DistrictBeijing 100094China Sino-Danish College University of Chinese Academy of SciencesBeijing100049China Laboratory of Proteomics Institute of BiophysicsChinese Academy of SciencesBeijingChina College of Life Sciences University of Chinese Academy of SciencesBeijing100049China Department of Interventional Oncology Beijing Ditan HospitalCapital Medical UniversityNo.8 Jingshun East StreetChaoyang DistrictBeijing100015China Joint Laboratory of Nanozymes in Zhengzhou University School of Basic Medical SciencesZhengzhou UniversityZhengzhou450001China

As one of the main tumor-infiltrating immune cell types, tumor-associated macrophages (TAMs) determine the efficacy of immunotherapy. However, limited knowledge about their phenotypically and functionally heterogeneous nature restricts their application in tumor immunotherapy. In this study, we identified a subpopulation of CD146+ TAMs that exerted antitumor activity in both human samples and animal models. CD146 expression in TAMs was negatively controlled by STAT3 signaling. Reducing this population of TAMs promoted tumor development by facilitating myeloid-derived suppressor cell recruitment via activation of JNK signaling. Interestingly, CD146 was involved in the NLRP3 inflammasome-mediated activation of macrophages in the tumor microenvironment, partially by inhibiting transmembrane protein 176B (TMEM176B), an immunoregulatory cation channel. Treatment with a TMEM176B inhibitor enhanced the antitumor activity of CD146+ TAMs. These data reveal a crucial antitumor role of CD146+ TAMs and highlight the promising immunotherapeutic approach of inhibiting CD146 and TMEM176B.

关键词： Tumor-associated macrophages CD146 Inflammasome TMEM176B Tumor immunotherapy

金黄色葡萄球菌CCT融合蛋白的表达及免疫活性研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

黑龙江八一农垦大学学报 2017年第6期29卷 40-44页

作者：马金柱张少铎程欣然叶玉婷何溪雨徐乐宋佰芬于立权于永忠朱战波崔玉东黑龙江八一农垦大学生命科学技术学院大庆163319

利用前期筛选的具有良好免疫原性的***表面Clfa蛋白免疫优势片段Clfais和TraP蛋白的融合重组蛋白,加入CTB分子内佐剂,以表达CTB-Clfais-TraP(CCT)蛋白和研究CCT免疫活性。实验采用重叠PCR方法将分子内佐剂CTB与Clfais-TraP基因串联,并将... 详细信息

利用前期筛选的具有良好免疫原性的***表面Clfa蛋白免疫优势片段Clfais和TraP蛋白的融合重组蛋白,加入CTB分子内佐剂,以表达CTB-Clfais-TraP(CCT)蛋白和研究CCT免疫活性。实验采用重叠PCR方法将分子内佐剂CTB与Clfais-TraP基因串联,并将CTB-Clfais-TraP插入到表达载体p ET32-a(+)上,构建p ET32-a(+)-CTB-Clfais-TraP重组质粒,经鉴定后,重组质粒转化到大肠埃希菌BL21中表达目的蛋白,利用Western blot方法对其鉴定,以ELISA方法检测CCT蛋白的免疫活性。实验结果表明表达的CCT蛋白大小为87.6 ku,CCT能与Clfais、TraP免疫小鼠血清发生反应,表明CCT具有免疫活性,不受CTB分子影响,这为进一步研究CTB提高Clfais-TraP蛋白的免疫活性奠定了良好基础。

关键词：金黄色葡萄球菌 CTB-Clfais-TraP 蛋白表达免疫活性

Protective humoral immunity in SARS-CoV-2 infected pediatric patients

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2020年第7期17卷 768-770页

作者： Yaguang Zhang Jin Xu Ran Jia Chunyan Yi Wangpeng Gu Pengcheng Liu xinran Dong Hao Zhou Bo Shang Shipeng cheng Xiaoyu Sun Jing Ye Xuezhen Li Jia Zhang Zhiyang Ling Liyan Ma Bingbing Wu Mei Zeng Wenhao Zhou Bing Sun State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of Sciences320 Yueyang RoadShanghai 200031China Children’s Hospital of Fudan University 399 Wanyuan RoadShanghai 201102China Shanghai Kehua Bio-Engineering Co. Ltd.1189 Qinzhou RoadShanghai 200233China

After the rapid spread of SARS-CoV-2 in Wuhan,China,at the beginning of 2020,about 1.5 million confirmed cases and over 80,000 deaths have been reported around 200 countries and territories all over the world and the number continues to ***,we still have limited knowledge of this new coronavirus,especially the interaction between SARS-CoV-2 and our immune system.

关键词： immunity pediatric infected