检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

高纯度大豆种子线粒体的分离

植物学通报 2006年第4期23卷 389-394页

作者：景新明尹广鹍中国科学院植物研究所北京100093

利用不连续Percoll梯度分离得到高纯度的大豆(Glycine max)种子线粒体。线粒体制剂中没有检测到胞液、过氧化物体和叶绿素的污染。线粒体的完整性达到97%以上,在0～4℃下至少稳定2小时。

关键词：大豆种子高纯度线粒体 Percoll

吸湿-回干处理对提高番茄种子萌发期间高温耐性的作用及其生理效应

在线全文

学校读者我要写书评

暂无评论

种子 2009年第2期28卷 34-38页

作者：郇述涛景新明中国科学院植物研究所北京100093 中国科学院研究生院北京100049

本试验以佳粉17番茄种子为材料,研究了吸湿-回干处理对番茄种子高温条件下萌发的发芽率、生物量、活力指数以及脂膜过氧化和保护酶活性的影响,旨在揭示吸湿-回干对提高番茄种子萌发的高温耐性的作用。种子经25℃吸湿30 h后自然晾干,然... 详细信息

本试验以佳粉17番茄种子为材料,研究了吸湿-回干处理对番茄种子高温条件下萌发的发芽率、生物量、活力指数以及脂膜过氧化和保护酶活性的影响,旨在揭示吸湿-回干对提高番茄种子萌发的高温耐性的作用。种子经25℃吸湿30 h后自然晾干,然后分别置33、35℃高温条件下萌发。置床30 h后测定抗氧化酶活性及脂膜过氧化指标,每天记录发芽种子数,10 d后测定生物量,计算发芽率、活力指数。结果表明,经吸湿-回干处理后,番茄种子的活力和生活力显著提高;超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)活性均有不同程度提高。电解质渗漏减缓,处理后的种子相对电导率(REC)减小,丙二醛(MDA)含量降低。由此可以推断,吸湿-回干处理在修补破损的生物膜的同时,增强抗氧化作用,抑制过氧化伤害,从而提高了番茄种子萌发的高温耐性。

关键词：番茄吸湿-回干高温耐性脂膜过氧化膜修补抗氧化酶

在线全文

学校读者我要写书评

暂无评论

富含多酚的麻栎种子中蛋白质提取的探讨

林业科学 2007年第2期43卷 26-30页

作者：辛霞景新明孙红梅于文文中国科学院植物研究所

采用单因素试验和正交试验的方法,对影响麻栎种子中蛋白质提取的主要因素进行分析,找出最佳提取条件,即采用含有1mmol.L-1EDTA,1mmol.L-1AsA,1mmol.L-1DTT,1mmol.L-1GSH,5mmol.L-1MgCl2,0.05%TritonX_100和20%甘油的50mmol.L-1磷酸钾缓冲液(pH值7.5),加入与材料等质量的不溶性聚乙烯多聚吡咯烷酮(PVPP)与材料共同研磨,即可获得大量的蛋白质。其中最主要的影响因素是PVPP的用量,PVPP阻止了多酚与蛋白质的结合,并将多酚沉淀,从而增加了蛋白质的溶解性。

关键词：麻栎种子蛋白质多酚 PVPP

Ecosystem evolution of seal colony and the influencing factors in the 20th century on Fildes Peninsula,West Antarctica

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2011年第9期23卷 1431-1436页

作者： jing Huang Liguang Sun xinming Wang Yuhong Wang Tao Huang Institute of Polar Environment University of Science & Technology of China Hefei 230026 China Guangzhou Institute of Geochemistry Chinese Academy of Science Guangzhou 510640 China Advanced Management Research Center Ningbo University Ningbo 315211 China

As the topmost predator in Antarctica,the seal is a unique indicator of Antarctic environment and climate *** this study,we collected a sediment core from the Fildes Peninsula of West Antarctica,and used cholesterol,cholestanol,epicoprostanol,coprostanol,and seal hair numbers as the proxy indicators of seal population size and phytol as of general vegetation,and we reconstructed the 20th century history of variation of the seal population and vegetation abundance on this *** sealing industry in the early 20th century caused the dramatic decline of seal population,and the ban of seal hunting since the 1960s led to its recovery of seal *** seal population during the past century was primarily controlled by human activities and krill *** reconstructed relation between seal population and vegetation abundance may offer new insights into Antarctic environment and ecology.

关键词： fecal sediment molecular marker human culture seal ecosystem climate

古近系油气初次运移有效性的主控因素——以珠江口盆地西江A和西江B构造圈闭油气勘探效果对比为例

在线全文

学校读者我要写书评

暂无评论

海洋地质前沿 2017年第10期33卷 40-48页

作者：刘道理杜忠明许新明吴静张琴陶禹董伟陈胜红中海石油(中国)有限公司深圳分公司研究院深圳518054

通过珠江口盆地西江主洼地区古近系油气勘探实例,提出了油气初次运移有效性与初次运移有效区的概念。初次运移是否有效取决于烃源岩排出之油气进入邻近砂体后能否顺利二次分配。初次运移有效性的主控因素取决于2点:一是发育与烃源岩接... 详细信息

通过珠江口盆地西江主洼地区古近系油气勘探实例,提出了油气初次运移有效性与初次运移有效区的概念。初次运移是否有效取决于烃源岩排出之油气进入邻近砂体后能否顺利二次分配。初次运移有效性的主控因素取决于2点:一是发育与烃源岩接触面积较大的规模性砂体;二是砂体中粗孔喉相对发育。前者可为初次运移有效性、提高运聚充注效率奠定基础;后者则决定了油气优先充注的运聚方向,其是决定初次运移最终有效的直接条件。据此,对比分析了西江A构造圈闭与西江B构造圈闭的钻探结果,并对西江A构造钻探失利进行了深入解剖,进而深化了该区古近系油气成藏规律的认识,且为进一步勘探寻找类似油气藏提供了参考和借鉴。

关键词：初次运移烃源岩规模性砂体粗孔喉古近系

在线全文

学校读者我要写书评

暂无评论

水稻种子耐干性机理的研究

粮食储藏 2007年第3期36卷 30-35,55页

作者：白桂香汪晓峰景新明林坚王文军北京林业大学 100083 中国科学院植物研究所 100093

以籼稻C38、粳稻京21、杂交稻秋优3为材料,进行种子超干储藏特性的研究,进而探讨种子耐干性的机理。通过超干水稻种子生活力和活力的比较,发现籼稻C38耐干性较强。超干有利于保持种子抗氧化酶系统的完整性,因此超干能提高种子的耐储性... 详细信息

以籼稻C38、粳稻京21、杂交稻秋优3为材料,进行种子超干储藏特性的研究,进而探讨种子耐干性的机理。通过超干水稻种子生活力和活力的比较,发现籼稻C38耐干性较强。超干有利于保持种子抗氧化酶系统的完整性,因此超干能提高种子的耐储性。对超干种子可溶性糖含量与组分进行分析,结果表明随着种子含水量的降低,蔗糖含量增高,多糖和葡萄糖含量降低,籼稻C38的蔗糖含量明显低于粳稻京21和杂交稻秋优3。用热重仪测定的种子含水量与烘干法得到的数据相吻合,用差示热量扫描仪测定束缚水/自由水的比例和玻璃化转变温度Tg,发现籼稻C38的Tg高于粳稻京21和杂交稻秋优3,籼稻C38束缚水/自由水比例大于粳稻京21和杂交稻秋优3,而束缚水/自由水的比例与耐干性成正相关。由此表明,耐干性在不同的水稻品种间有明显的差异,籼稻C38的耐干性较强。

关键词：水稻种子活力超干耐干性可溶性糖抗氧化酶热重仪差示热量扫描

Model assessment of nutrient removal via planting Sesuvium portulacastrum in floating beds in eutrophic marine waters:the case of aquaculture areas of Dongshan Bay

在线全文

学校读者我要写书评

暂无评论

Acta Oceanologica Sinica 2019年第12期38卷 91-100页

作者： Xuehai Liu xinming Pu Donglian Luo jing Lu Zili Liu First Institute of Oceanography Ministry of Natural ResourcesQingdao 266061China Fujian Fishery Research Institute Xiamen 361012China Laboratory of Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Laboratory for Regional Oceanography and Numerical Modeling Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Key Laboratory of Marine Science and Numerical Modeling Ministry of Natural ResourcesQingdao 266061China Key Laboratory of Science and Engineering for the Marine Ecological Environment Ministry of Natural ResourcesQingdao 266061China

Many coastal seas are severely eutrophic and required to reduce nutrient concentrations to meet a certain water quality *** proposed a method for nutrient removal by planting Sesuvium portulacastrum at the water surface using the floating beds in the aquaculture area of the Dongshan Bay as an example,which is an important net-cage culture base in China and where dissolved inorganic nitrogen(DIN)and dissolved inorganic phosphate(DIP)reach 0.75 mg/L and 0.097 mg/L,respectively far exceeding China’s Grade IV water quality *** simulations were taken using the ecological model,field observations and field plantation experimental results to assess the environmental restoration effects of planting *** at some certain spatial *** field experiments suggested that the herbs can absorb 377 g/m^2 nitrogen and 22.9 g/m^2 phosphorus in eight months with an inserting density of^60 shoot/m^*** numerical experiments show that the greater the plantation area is,the more nutrient *** in^12%of the study area could lower nutrients to the required Grade II standards,i.e.,0.2 mg/L

关键词： phytoremediaton Sesuvium portulacastrum ecological model nutrient removal

Experimental investigation of the thermally induced vibration of a space boom section

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2015年第4期58卷 58-66页

作者： SU xinming ZHANG JunHui WANG jing BI YanQiang QIE DianFu XIANG ZhiHai XUE MingDe Beijing Institute of Spacecraft Environment Engineering College of Architecture and Civil Engineering North China University of Technology Science and Technology on Reliability and Environmental Engineering Laboratory Beijing Institute of Spacecraft Environment Engineering Key Laboratory of Applied Mechanics School of Aerospace Engineering Tsinghua University

Thermally induced vibration(TIV)is a typical failure of large-scale space *** paper reports a laboratory experiment that aims to investigate this unusual structural behavior of complex engineering *** the help of a Fourier finite element program,the fixing of a space boom section is well designed so that the TIV can be successfully *** the observed torsional vibration mode is different from the bending vibration mode predicted by the classic theory based on a simple beam model,it can be successfully explained by the general thermal structural interaction *** demonstrates the validity of the theoretical model and the necessity of using finite element program to analyze complex engineering structures.

关键词： thermal-structural interaction vibration experiment

Ectopic expression of OsNF-YA8,an endosperm-specific nuclear factor Y transcription-factor gene,causes vegetative and reproductive development defects in rice

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2023年第6期11卷 1719-1730页

作者： Baixiao Niu jing Xu Zhiguo E Zhenyu Zhang xinming Lu Chen Chen Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory Agricultural College of Yangzhou UniversityYangzhou 225009JiangsuChina Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina Key Laboratory of Plant Functional Genomics of the Ministry of Education Agricultural College of Yangzhou UniversityYangzhou 225009JiangsuChina Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 311400ZhejiangChina

Nuclear factor Y(NF-Y),a group of conserved transcription-factor complexes that consist of NF-YA,B,and C subunits,is essential for developmental regulation and for responses to environmental changes in *** previously found that some NF-Y genes,such as OsNF-YA8,were expressed specifically in the endosperm of *** the present study,overexpression of OsNF-YA8 in rice resulted in reduced plant height due to suppressed cell elongation in ***(GA)biosynthetic genes,including OsCPS1,OsGA20ox1,and OsGA20ox2,were ***-YA8 bound to the promoters of these genes to repress their *** GA content was decreased in OsNF-YA8 overexpressors,whose dwarf phenotype could be partially rescued by exogenous GA *** findings suggested that ectopic expression of OsNF-YA8 causes defective GA biosynthesis in vegetative *** date in OsNF-YA8 overexpressors was delayed,especially under short-day ***8 bound to the promoter of Heading Date 3a(Hd3a),the florigen gene in rice,to negatively regulate *** ectopic activation or knockout of OsNF-YA8 impaired seed development,as indicated by reduced seed size and increased grain *** results suggest that ectopic expression of the endosperm-specific OsNF-YA8 in rice disrupts both vegetative and reproductive development.

关键词： Rice OsNF-YA8 Plant height Flowering time Seed development