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Neotuberostemonine and tuberostemonine ameliorate pulmonary fibrosis through suppressing TGF-β and SDF-1 secreted by macrophages and fibroblasts via the PI3K-dependent AKT and ERKpathways

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Chinese Journal of Natural Medicines 2023年第7期21卷 527-539页

作者： FU San SONG Xianrui HU Yingying ZHU Qingwei lV xinmiao TANG Xiaoyan ZHANG Mian School of Traditional Chinese Pharmacy China Pharmaceutical UniversityNanjing 211198China

Activated fibroblasts and M2-polarized macrophages may contribute to the progression of pulmonary fibrosis by forming a positive feedback *** study was aimed to investigate whether fibroblasts and macrophages form this loop by secreting SDF-1 and TGF-β and the impacts of neotuberostemonine(NTS)and tuberostemonine(TS).Mice were intratracheally injected with 3 U·kg^(-1)bleomycin and orally administered with 30 mg·kg^(-1)NTS or *** pulmonary fibroblasts(PFBs)and MH-S cells(alveolar macrophages)were used in *** animal experiments showed that NTS and TS improved fibrosis related indicators,inhibited fibroblast activation and macrophage M2 polarization,and reduced the levels of TGF-β and SDF-1 in alveolar lavage *** experiments showed that TGF-β1 may activated fibroblasts into myofibroblasts secreting SDF-1 by activating the PI3K/AKT/HIF-1αand PI3K/PAK/RAF/ERK/HIF-1α*** was also found for the first time that SDF-1 was able to directly polarize macrophages into M2 phenotype secreting TGF-β through the same pathways as mentioned ***,the results of the cell coculture confirmed that fibroblasts and macrophages actually developed a feedback loop to promote fibrosis,and the secretion of TGF-β and SDF-1 was crucial for maintaining this *** and TS may disturb this loop through inhibiting both the PI3K/AKT/HIF-1αand PI3K/PAK/RAF/ERK/HIF-1α pathways to improve pulmonary *** and TS are stereoisomeric alkaloids with pyrrole[1,2-ajazapine skeleton,and their effect on improving pulmonary fibrosis may be largely attributed to their parent ***,this study found that inhibition of both the AKT and ERK pathways is essential for maximizing the improvement of pulmonary fibrosis.

关键词： Fibroblast Macrophage SDF-1 PI3K/AKT PI3K/PAK/RAF/ERK Pulmonary fibrosis Pyrrole[1,2-a]azapine skeleton

Biomimetic nanomedicines for precise atherosclerosis theranostics

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Acta Pharmaceutica Sinica B 2023年第11期13卷 4442-4460页

作者： Ying Tao xinmiao lan Yang Zhang Chenxing Fu lu liu Feng Cao Weisheng Guo Department of Minimally Invasive Interventional Radiology Key Laboratory of Molecular Target&Clinical PharmacologySchool of Biomedical Engineering&the Second Affiliated HospitalGuangzhou Medical UniversityGuangzhou 510260China School of Pharmaceutical Sciences Capital Medical UniversityBeijing 100069China Department of Cardiology the Second Medical Center&National Clinical Research Center for Geriatric DiseasesChinese PLA General HospitalBeijing 100853China Department of Cardiology and laboratory of Heart Center Zhujiang HospitalSouthern Medical UniversityGuangzhou 510280China Department of Pharmacology and Pharmacy Li Ka Shing Faculty of Medicinethe University of Hong KongHong Kong SAR 999077China

Atherosclerosis(AS)is a leading cause of the life-threatening cardiovascular disease(CVD),creating an urgent need for efficient,biocompatible therapeutics for diagnosis and *** nanomedicines(b NMs)are moving closer to fulfilling this need,pushing back the frontier of nano-based drug delivery systems *** review seeks to outline how these nanomedicines(NMs)might work to diagnose and treat atherosclerosis,to trace the trajectory of their development to date and in the coming years,and to provide a foundation for further discussion about atherosclerotic theranostics.

关键词： Nanotheranostics Atherosclerosis Biomimetic nanomedicines Drug delivery Passive targeting Active targeting Plaques Diagnosis and treatment

一株枯草芽孢杆菌噬菌体Bac-S的生物学特性及全基因组序列分析

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微生物学报 2024年第9期64卷 3379-3392页

作者：赵欣卓王鑫淼王丽艳吴楠李喜梅于镇华荆瑞勇刘俊杰黑龙江八一农垦大学生命科学技术学院黑龙江省寒区环境微生物与农业废弃物资源化利用重点实验室黑龙江大庆163319 中国科学院东北地理与农业生态研究所黑土区农业生态重点实验室黑龙江哈尔滨150081 农业农村部东北平原农业绿色低碳重点实验室黑龙江大庆163319

【目的】枯草芽孢杆菌(Bacillus subtilis)施用于土壤可促进植物生长,但针对土壤中枯草芽孢杆菌噬菌体的研究鲜有报道,因此从土壤中分离出枯草芽孢杆菌噬菌体,并深入研究其生物学特性及基因组特征,不仅有助于枯草芽孢杆菌的实际应用,还... 详细信息

【目的】枯草芽孢杆菌(Bacillus subtilis)施用于土壤可促进植物生长,但针对土壤中枯草芽孢杆菌噬菌体的研究鲜有报道,因此从土壤中分离出枯草芽孢杆菌噬菌体,并深入研究其生物学特性及基因组特征,不仅有助于枯草芽孢杆菌的实际应用,还能丰富枯草芽孢杆菌噬菌体相关生物信息库。【方法】以枯草芽孢杆菌SM13为宿主,从土壤中分离培养出一株噬菌体Bac-S,对其进行生物学特性测定、全基因组序列特征、基因功能注释和系统发育分析。【结果】透射电镜显示,噬菌体Bac-S头部直径约为43 nm,尾部极短无法测量;该噬菌体的最佳感染复数(multiplicity of infection,MOI)为0.1;一步生长曲线显示,潜伏期约为10 min,裂解量为30 PFU/cell;宿主谱广泛,能实现跨属侵染;测序结果显示其基因组长度为150019 bp,G+C含量为42.6%;该噬菌体共有237个开放阅读框(open reading frames,ORFs),BlASTn比对显示该噬菌体与NCBI数据库中其他芽孢杆菌噬菌体相似性较高。【结论】从土壤中分离出了一株具有潜伏期短、宿主谱广泛、耐受高温、不耐紫外光照射的特征枯草芽孢杆菌噬菌体,拓宽了对枯草芽孢杆菌噬菌体的认知。

关键词：枯草芽孢杆菌噬菌体生物学特性基因组

益骨汤对去势大鼠骨密度及经典BMP2信号通路的影响

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中国现代医生 2024年第6期62卷 73-78,83页

作者：林晓芳方芳李桂锦姚新苗浙江省台州医院骨科浙江临海317000 浙江中医药大学第三临床医学院针灸神经生物学实验室浙江杭州310053 浙江中医药大学附属第三医院骨科浙江杭州3100531

目的观察益骨汤对去势大鼠骨组织中经典骨形态发生蛋白(bone morphogenetic protein,BMP)2信号通路的影响,旨在探讨益骨汤防治骨质疏松症(osteoporosis,OP)的作用机制。方法将44只12周龄雌性Wistar大鼠随机分为四组,每组11只。正常组不... 详细信息

目的观察益骨汤对去势大鼠骨组织中经典骨形态发生蛋白(bone morphogenetic protein,BMP)2信号通路的影响,旨在探讨益骨汤防治骨质疏松症(osteoporosis,OP)的作用机制。方法将44只12周龄雌性Wistar大鼠随机分为四组,每组11只。正常组不进行任何处理,模型组、戊酸雌二醇组和益骨汤组大鼠进行去势手术以模拟骨质疏松。造模成功后,分别以10ml/kg双蒸水灌胃(正常组、模型组)、0.36mg/kg戊酸雌二醇水溶液灌胃及10ml/kg益骨汤灌胃,1次/d。12周后,双能X射线骨密度仪测定股骨骨密度(bone mineral density,BMD),实时聚合酶链反应(polymerase chain reaction,PCR)检测BMP2经典信号通路BMP2、Smad1、Runx2、OSX基因的表达,免疫组织化学法、Western blot法检测BMP2经典信号通路BMP2、Smad1、Runx2、OSX蛋白的表达量。结果①双能X射线骨密度仪测定股骨骨密度,结果显示,与模型组比较,益骨汤组大鼠的BMD升高(Plot法检测:与模型组比较,益骨汤组大鼠的Smad1、Runx2、OSX蛋白含量升高(P<0.05)。结论益骨汤可能通过激活BMP2经典信号通路及抑制Noggin的高表达,促进骨形成和防治骨质疏松。

关键词：益骨汤去势大鼠骨质疏松 BMP2经典信号通路 Noggin

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Occurrence, spatial distribution and ecological risks of antibiotics in soil in urban agglomeration

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Journal of Environmental Sciences 2023年第3期125卷 678-690页

作者： Min li lei Yang Haw Yen Fangkai Zhao xinmiao Wang Tianhui Zhou Qingyu Feng liding Chen State Key laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China School of Forestry and Wildlife Sciences Auburn UniversityTexas 36849USA School of Ecology and Environmental Science Yunnan UniversityKunming 650500China

Antibiotics in soil environment are regarded as emerging pollutants and have introduced increasing risks to soil ecosystem and human health in rapid urbanization areas. Identifying the occurrence and spatial variability of antibiotics in soils is an urgent issue in sustaining soil security. In this study, antibiotics in soils were investigated and analyzed in BeijingTianjin-Hebei urban agglomeration. The occurrence, spatial distribution, and related affecting factors of antibiotics in soils were identified and ecological risks of antibiotics in soil environment were assessed. Results showed that(1) The mean concentration of soil antibiotics in Beijing-Tianjin-Hebei urban agglomeration was 21.79 μg/kg. land use substantially affected the occurrence and concentration of antibiotics in soils. Concentrations of antibiotics in cropland and orchard soils were 2-3 times higher than the other land use types.(2)The concentrations of antibiotics in soils in Beijing-Tianjin-Hebei urban agglomeration presented a spatial pattern of high values in southeast, and low values in northwest. Spatial variability of antibiotics in soils was closely related to the application of organic fertilizer and wastewater irrigation as well as topographical features. Furthermore, soil properties and land management policy had substantial influences on soil antibiotics, and soil heavy metals may aggravate the accumulation of antibiotics in soils.(3) Ecological risks assessment of antibiotics in soils demonstrated that erythromycin(ERY), sulfamethoxazole(SMX), and doxycycline(DOX) may introduce high risks to soil ecosystem health, and more attention should be paid to the areas with intensive human activities that had potential high risk to soil ecosystem health. This study suggests that scientific land and soil management should be considered to prevent soil antibiotic pollution and sustain soil security in urban agglomeration.

关键词： Antibiotics Urban agglomeration land use land management Urban-rural environmental gradient

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全断面富水粉细砂层盾尾原位改造技术研究

土工基础 2024年第2期38卷 178-180,191页

作者：张维夏晓谦陈少林孙恒黄新淼中交二航局第三工程有限公司江苏镇江212000 中交二航局(成都)建设工程有限公司成都610218 中交第二航务工程局有限公司武汉430040

盾尾油脂孔的不合理布设易导致间距过大处尾刷磨损严重,出现固定渗漏点,严重威胁盾构施工安全。为了弥补这一缺陷,以福州地铁滨海快线大数据站-滨海新城站区间工程为依托,首先对盾尾改造原因、改造前注浆止水加固工作进行了详细说明。... 详细信息

盾尾油脂孔的不合理布设易导致间距过大处尾刷磨损严重,出现固定渗漏点,严重威胁盾构施工安全。为了弥补这一缺陷,以福州地铁滨海快线大数据站-滨海新城站区间工程为依托,首先对盾尾改造原因、改造前注浆止水加固工作进行了详细说明。其次从风险、工期、成本3个方面对不同盾尾原位改造方案进行对比分析,结果表明通过设计一块特制尾刷并与外置槽钢焊接明显优于其他几类方案。通过上述措施,成功在无水环境中安全且高效地完成了盾尾改造,新增油脂孔可正常泵送油脂至腔内,极大保障了后续施工安全。相关经验可为后续类似工程提供参考。

关键词：盾尾改造特制尾刷富水粉细砂层注浆法

重庆市某区青少年不良健康行为与焦虑抑郁的相关分析

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健康教育与健康促进 2024年第3期19卷 234-236,283页

作者：莫敏张莉梅印德娇敬新苗重庆市北碚区妇幼保健计划生育服务中心重庆400700

目的分析重庆市某区青少年不良健康行为和焦虑、抑郁的现状,为制定青少年焦虑、抑郁的防控策略提供科学依据。方法采用分层整群抽样方法对重庆市某区6所学校的2 632名青少年进行网络问卷调查。结果有16.8%的青少年曾吸烟;有61.2%的... 详细信息

目的分析重庆市某区青少年不良健康行为和焦虑、抑郁的现状,为制定青少年焦虑、抑郁的防控策略提供科学依据。方法采用分层整群抽样方法对重庆市某区6所学校的2 632名青少年进行网络问卷调查。结果有16.8%的青少年曾吸烟;有61.2%的青少年曾饮酒;7.8%的青少年每周平均每天睡眠logistic回归分析显示:性别、曾吸烟、曾饮酒、一周每天平均睡眠时间<6小时、网络成瘾、自杀意念是焦虑的影响因素;曾饮酒、一周每天平均睡眠时间<6小时、网络成瘾、自杀意念是抑郁的影响因素。结论青少年的不健康心理行为问题令人担忧,需采取有效的综合措施进行干预。

关键词：青少年不良健康行为焦虑抑郁

Models for economic evaluation of multi-purpose apple harvest platform and software development

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International Journal of Agricultural and Biological Engineering 2019年第1期12卷 74-83页

作者： Zhao Zhang Zhaohua Zhang xinmiao Wang Hu liu Yuanjie Wang Weijie Wang Department of Agricultural and Biological Engineering Pennsylvania State UniversityState CollegePA 16803USA College of Economics and Management Shandong Agricultural UniversityTai’an 271018ShandongChina School of Food Science&Biotechnology Zhejiang Gongshang UniversityHangzhou 310018China Northwest Institute of Eco-Environment and Resources Chinese Academy of SciencesLanzhou 730000China Agricultural Information Institute Chinese Academy of Agricultural SciencesBeijing 100081China State Grade Dezhou Power Supply Company Dezhou 253000ShandongChina

An increasing number of *** growers are now interested in using harvest platforms to increase harvest productivity,expand labor pool,and alleviate tough working *** maximize benefits,functions of thinning,pruning&training,and infield sorting have been or are to be incorporated into harvest *** growers are most concerned with economic benefits,few cost-benefit studies had been conducted on different *** the meantime,economic analysis procedure is complex and each analysis is for one specific machine(not for general purposes).No software has been developed as a general and ready-to-use tool for growers and researchers for the platform economic *** this study,platforms,both available on the marketplace and developed in lab as pilot trials,were *** and benefits models were then established,based on which multi-purpose apple harvest platform economic evaluation software(iMPAHP)was developed(capable of evaluating a wide variety of apple harvest platforms).A case study(machine cost of$100000,accommodating 6 workers,processing apple incidence of 10%with 90%sort-out rate,and harvest,thinning,and pruning&training productivity increase by 40%,50%,and 60%,respectively)based on iMPAHP demonstrated that infield sorting,harvest,thinning,and pruning and training accounted for 48.4%,23.9%,14.3%,and 13.4%of the total benefits,*** the case that the platform was in all-four-purpose-application,the net present value(NPV)analysis of a 10-year investment showed a positive return of$***,without infield sorting function,the NPV resulted in a negative value,indicating a loss for the machine *** incorporating the modular infield sorting system certainly increased the overall machine investment by$30000,the benefits outweighed the costs.

关键词： multi-purpose apple harvest platform economic evaluation infield sorting pruning thinning training

粪肠球菌铁稳态维持对其生存和致病性影响的研究进展

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口腔生物医学 2024年第4期15卷 240-244页

作者：甄雨琦姜鑫淼白玛曲珍孟秀萍吉林大学口腔医院牙体牙髓病科吉林长春130021

粪肠球菌(***)是一种革兰氏阳性细菌,是引起难治性根尖周炎的主要细菌微生物。铁是***生存必不可少的微量元素,为了获取宿主铁,***进化出多种机制,并且还可耐受对大多数细菌有毒性的高铁浓度。***能够感知环境中铁水平的变化,并作出相... 详细信息

粪肠球菌(***)是一种革兰氏阳性细菌,是引起难治性根尖周炎的主要细菌微生物。铁是***生存必不可少的微量元素,为了获取宿主铁,***进化出多种机制,并且还可耐受对大多数细菌有毒性的高铁浓度。***能够感知环境中铁水平的变化,并作出相应调整,这使得其能够在不同宿主生态位中成长并引发感染,因此本文综述了***铁稳态的维持机制、铁稳态维持对***致病性的影响以及通过调节铁稳态抑制***活性的相关研究进展,为防治根尖周炎等感染性疾病提供理论依据。

关键词：粪肠球菌铁铁稳态