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Agrobacterium–mediated Transformation of Kalanchoe laxiflora

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Horticultural Plant Journal 2019年第5期5卷 221-228页

作者： Xiling Wang xinlu chen Qunkang cheng Kaikai Zhu Xiaohan Yang Zongming cheng Department of Plant Sciences University of TennesseeKnoxvilleTN37996USA College of Biotechnology Southwest UniversityChongqing400715China Department of Entomology and Plant Pathology University of TennesseeKnoxvilleTN37996USA College of Horticulture Nanjing Agricultural UniversityNanjing210095China Biosciences Division Oak Ridge National LaboratoryOak RidgeTN37831-6422USA

Plants with crassulacean acid metabolism(CAM) generally utilize water 20%–80% more efficiently than non-CAM plants. The whole genomes of several CAM plants have been sequenced or are being sequenced. For effective genome characterization and genome editing of CAM plants,an efficient transformation system is essential. In this study, we developed an Agrobacterium-mediated transformation protocol for Kalanchoe laxiflora, an obligate CAM plant,by optimizing several factors affecting the transformation efficiency. Agrobacterium strains AGL1, C58, EHA105,and GV3101 were all suitable for K. laxiflora transformation. Fifty-nine percent of the leaf explants yielded kanamycin-resistant and GUS-positive shoots. Polymerase chain reaction and quantitative real-time PCR(qRT-PCR) using gus A-, gus Plus-, npt II-and hpt-specific primers confirmed that the transgenes were integrated into K. laxiflora genome and expressed. This efficient transformation system will allow effective functional characterization of genes through over-or down-expression, knockout, or genome editing.

关键词： Kalanchoe laxiflora Agrobacterium-mediated transformation CAM plants

伟大建党精神融入医学生人文素质培育的路径研究

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中医药管理杂志 2024年第1期32卷 216-218页

作者：林昕潞陈翔浙江中医药大学浙江杭州310053

伟大建党精神蕴含着丰富的育人价值,是医学生人文素质培育的生动教材。文章在探讨两者内在逻辑的基础上,分析医学生人文素质培育的现状及面临的困境,并结合实际提出伟大建党精神融入医学生人文素质培育的路径,即发挥榜样示范作用、完善... 详细信息

伟大建党精神蕴含着丰富的育人价值,是医学生人文素质培育的生动教材。文章在探讨两者内在逻辑的基础上,分析医学生人文素质培育的现状及面临的困境,并结合实际提出伟大建党精神融入医学生人文素质培育的路径,即发挥榜样示范作用、完善实践育人体系、丰富教育载体形式等。

关键词：伟大建党精神医学生人文素质培育

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低/超低介电常数聚酰亚胺的研究进展

塑料工业 2024年第5期52卷 31-36,71页

作者：李欣膂梁昊陈婷徐伊凡陈嘉敏刘学清陈风刘继延江汉大学光电化学材料与器件教育部重点实验室湖北武汉430056 江汉大学光电材料与技术学院湖北武汉430056

聚酰亚胺(PI)是综合性能最佳的有机高分子材料之一,被认为是最具潜力的柔性电子器件基材。下一代电子元器件正向低维度、大规模及精细化集成发展,对作为层间绝缘材料的PI提出了更高的要求,而普通芳香族PI介电常数偏高,无法直接应用于微... 详细信息

聚酰亚胺(PI)是综合性能最佳的有机高分子材料之一,被认为是最具潜力的柔性电子器件基材。下一代电子元器件正向低维度、大规模及精细化集成发展,对作为层间绝缘材料的PI提出了更高的要求,而普通芳香族PI介电常数偏高,无法直接应用于微电子工业领域。因此,当前急需开发降低PI材料介电常数的新方法。本文综述了近年来低/超低介电常数PI的相关研究和系统性工作,希望这些综合性归纳能为低、超低介电常数PI的构筑提供新方案,也为PI的加工工艺提供新的视角。

关键词：低/超低介电常数聚酰亚胺本征结构多孔结构

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胰岛素抵抗与阿尔茨海默病的关联性研究进展

中国医药 2024年第5期19卷 777-781页

作者：朱徐锐陈昕露顾小萍南京医科大学鼓楼临床医学院南京鼓楼医院麻醉科南京210008 南京大学医学院附属鼓楼医院麻醉科南京210008

随着中国老龄化人口的比例不断增加,阿尔茨海默病的发病率也随之升高。阿尔茨海默病作为最常见的神经退行性疾病,会表现出不可逆转的认知功能障碍。多项研究表明,胰岛素抵抗与阿尔茨海默病的β淀粉样蛋白异常积累、tau蛋白的异常以及神... 详细信息

随着中国老龄化人口的比例不断增加,阿尔茨海默病的发病率也随之升高。阿尔茨海默病作为最常见的神经退行性疾病,会表现出不可逆转的认知功能障碍。多项研究表明,胰岛素抵抗与阿尔茨海默病的β淀粉样蛋白异常积累、tau蛋白的异常以及神经炎症等发病机制具有关联性。本文系统总结了胰岛素抵抗与阿尔茨海默病的关联性及其可能机制。

关键词：阿尔茨海默病胰岛素抵抗关联性 β淀粉样蛋白 tau蛋白神经炎症

Increasing Anthropogenic Mercury Pollution over the Last 200 Years Revealed by Lagoonal Sediments from Hainan Island,South China

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Chinese Geographical Science 2023年第6期33卷 1127-1140页

作者： LI Yanting XUE Jibin chen Jingqiang LU Yi MA xinlu School of Geographical Sciences South China Normal UniversityGuangzhou510631China

The toxic heavy metal mercury(Hg)has significantly enhanced the global Hg cycle influenced by human activities over the last century.In this study,we presented a high-resolution Hg deposition history between∼1780 and 2015 AD in a sediment core from Xincun Lagoon,located in the southeastern Hainan Island,South China,and analyzed it in conjunction with geochemical elements,grain-size distribution,organic matter,and HYSPLIT backward trajectory simulation.The objective was to investigate the influencing factors affecting historical Hg deposition in relatively remote regions and assess the extent of the effects of natural background and human activities.The results showed that the Hg in the sediment was deposited primarily through atmospheric deposition,which was closely related to regional and even global human activities.Anthropogenic Hg contamination increased gradually from the 1830s to 1850s,possibly due to Hg emissions from Opium Wars I and II occurring in southeastern China.High broad peaks of anthropogenic Hg were observed during the 1910s to 1950s and in the 1980s,likely associated with the two world wars and modern Chinese wars.In addition,a further sharp increase in anthropogenic Hg from the mid-1970s to the present occurred,likely originating from the intense industrial activities in China triggered by the reform and opening-up policy of China in 1978 and some countries in Southeast Asia.

关键词： Mercury deposition pollution history anthropogenic impact Xincun Lagoon Hainan,China

Innovative design and optimization of a two-dimensional deployable nine-grid planar antenna mechanism with a flat reflection surface

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Chinese Journal of Aeronautics 2023年第11期36卷 529-550页

作者： Bo chen Ze JIANG xinlu WEI Luyao GUO Xin ZHOU Yundou XU Junjie QIAN Yongsheng ZHAO Parallel Robot and Mechatronic System Laboratory of Hebei Province Yanshan UniversityQinhuangdao 066004China Key Laboratory of Advanced Forging&Stamping Technology and Science of Ministry of National Education Yanshan UniversityQinhuangdao 066004China Space Structure and Mechanism Technology Laboratory of China Aerospace Science and Technology Group Co.Ltd Shanghai 201109China Aerospace System Engineering Shanghai Shanghai 201109China

With the development of the aerospace industry,space missions are becoming more complicated and diversified,and there is a demand for antenna mechanisms with a larger physical aperture.In this paper,a planar deployable mechanism is proposed,which can form a flat reflection surface with a small gap between plates.To this end,a novel large-scale two-dimensional deployable nine-grid planar antenna mechanism is designed.First,two antenna folding schemes and four supporting mechanism schemes are proposed.Through comparison analysis,the antenna configuration scheme with the best comprehensive performance is selected.A kinematic model of the deployable mechanism is established,and its kinematic characteristics are analyzed.Then,the correctness of the kinematic model is verified by comparing the analytical and simulation results of the kinematic model.Subsequently,a finite element model of the antenna is developed.Based on the response surface method,the structural parameters of the support rods of the antenna are optimized,and a set of optimized solutions with lightweight and high fundamental frequency characteristics are obtained.Finally,a prototype of the proposed nine-grid planar antenna is fabricated.The feasibility of the deployment principle and the rationality of the designed mechanism are verified by deployment experiments.

关键词： Design optimization Kinematics Planar antenna Structural design Two-dimensional expansion

^(18)F-FAPI与^(18)F-FDG PET/CT在腹膜癌诊断中的价值对比

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中华肿瘤防治杂志 2024年第5期31卷 268-273页

作者：刘宇衡黄浩林子凯黄炯强蔡建张静陈劲松何曦王欣璐广州医科大学附属第一医院核医学科广东广州510120 广州医科大学附属第一医院胃肠外科广东广州510120 深圳市第二人民医院胃肠外科广东深圳518025

目的比较^(18)F-FAPI PET/CT与^(18)F-FDG PET/CT检查对腹膜癌的诊断效能。方法选取2020-11-01-2022-08-31广州医科大学附属第一医院收治的42例腹膜癌患者作为研究对象。其中,男13例,女29例;中位年龄为61岁(53.75~68.00岁);包括胃癌12例... 详细信息

目的比较^(18)F-FAPI PET/CT与^(18)F-FDG PET/CT检查对腹膜癌的诊断效能。方法选取2020-11-01-2022-08-31广州医科大学附属第一医院收治的42例腹膜癌患者作为研究对象。其中,男13例,女29例;中位年龄为61岁(53.75~68.00岁);包括胃癌12例,卵巢癌10例,结直肠癌9例,肝癌5例,肺癌2例,胰腺癌1例,十二指肠癌1例,肾癌1例,原发性腹膜癌1例。所有患者均进行^(18)F-FAPI和^(18)F-FDG全身PET/CT显像检查。根据对同1个患者的2次PET/CT显像结果统计腹膜癌的检出率、最大标准摄取值(SUV_(max))和腹膜癌指数,并使用配对样本t检验比较2种检查结果。结果^(18)F-FAPI PET/CT对腹膜癌的检出率(100.0%)高于^(18)F-FDG PET/CT(73.8%),P<0.001。根据半定量分析,42例腹膜癌患者^(18)F-FAPI PET/CT的SUV_(max)[7.1(5.0~11.5)]高于^(18)F-FDG PET/CT[4.8(0.4~6.5)],t=6.498,P<0.001;卵巢癌和胃癌腹膜转移患者在^(18)F-FAPI PET/CT图像的SUV_(max)高于^(18)F-FDG PET/CT(t=6.165,P<0.001;t=4.747,P=0.001)。根据视觉评估,^(18)F-FAPI PET/CT图像中腹膜癌病灶的腹膜癌指数[18.0(9.0~27.0)]高于^(18)F-FDG PET/CT[8.5(2.0~25.5)],t=4.378,P<0.001。结论^(18)F-FAPI PET/CT对腹膜癌的诊断效能优于^(18)F-FDG PET/CT,尤其对胃癌和卵巢癌的腹膜转移瘤。

关键词：腹膜癌 ^(18)F-FAPI PET/CT ^(18)F-FDG PET/CT 最大摄取值腹膜癌指数

Efficient de novo assembly and modification of large DNA fragments

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Science China(Life Sciences) 2022年第7期65卷 1445-1455页

作者： Shuangying Jiang Yuanwei Tang Liang Xiang xinlu Zhu Zelin Cai Ling Li Yingxi chen Peishuang chen Yuge Feng Xin Lin Guoqiang Li Jafar Sharif Junbiao Dai CAS Key Laboratory of Quantitative Engineering Biology Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic GenomicsShenzhen Institute of Synthetic BiologyShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China RIKEN Center for Integrative Medical Sciences(IMS) Developmental Genetics LaboratoryYokohama 230-0045Japan

Synthetic genomics has provided new bottom-up platforms for the functional study of viral and microbial genomes.The construction of the large,gigabase(Gb)-sized genomes of higher organisms will deepen our understanding of genetic blueprints significantly.But for the synthesis and assembly of such large-scale genomes,the development of new or expanded methods is required.In this study,we develop an efficient pipeline for the construction of large DNA fragments sized 100 kilobases(kb)or above from scratches and describe an efficient method for“scar-free”engineering of the assembled sequences.Our method,therefore,should provide a standard framework for producing long DNA molecules,which are critical materials for synthetic genomics and metabolic engineering.

关键词： DNA assembly budding yeast large DNA fragments sequence modification DNA synthesis Synthetic Biology

NITROGEN USE AND MANAGEMENT IN ORCHARDS AND VEGETABLE FIELDS IN CHINA: CHALLENGES AND SOLUTIONS

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Frontiers of Agricultural Science and Engineering 2022年第3期9卷 386-395页

作者： Xueqiang ZHU Peng ZHOU Peng MIAO Haoying WANG xinlu BAI Zhujun chen Jianbin ZHOU College of Natural Resources and Environment Northwest A&F UniversityYangling 712100China Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China Ministry of Agriculture and Rural Affairs of ChinaYangling 712100China.

China is the largest producer and consumer of fruits and vegetables in the world.Although the annual planting areas of orchards and vegetable fields(OVF)account for 20%of total croplands,they consume more than 30%of the mineral nitrogen fertilizers in China and have become hotspots of reactive N emissions.Excess N fertilization has not only reduced the N use efficiency(NUE)and quality of grown fruits and vegetables but has also led to soil acidification,biodiversity loss and climate change.Studies using 15N labeling analysis showed that the recovery rate of N fertilizer in OVFs was only 16.6%,and a high proportion of fertilizer N resided in soils(48.3%)or was lost to the environment(35.1%).Nitrate accumulation in the soil of OVFs is the main fate of N fertilizer in northern China,which threatens groundwater quality,while leaching and denitrification are the important N fates of N fertilizer in southern China.Therefore,taking different measures to reduce N loss and increase NUE based on the main pathways of N loss in the various regions is urgent,including rational N fertilization,substituting mineral N fertilizers with organic fertilizers,fertigation,and adding mineral N fertilizers with urease inhibitors and nitrification inhibitors.

关键词： nitrogen fate nitrogen fertilizer orchards vegetable fields