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Mixture of five herbal extracts ameliorates pioglitazone-induced aggravation of hepatic steatosis via activating the adiponectin receptor 2/AMP-activated protein kinase signal pathway in diabetic KKAy mice

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Journal of Traditional Chinese Medicine 2017年第5期37卷 588-598页

作者： Wang Haiyan Li Linyi Qin Lingling Wang Dongchao Jiang Yueying wu xinli Xu Tunhai Liu Tonghua Health-cultivation Laboratory of the Ministry of Education Beijing University of Chinese Medicine Key Laboratory of Upper Airway Dysfunction-related Cardiovascular Diseases Beijing Institute of Heart Lung and Blood Vessel Diseases Beijing Anzhen Hospital Capital Medical University

OBJECTIVE: To assess the effect of a mixture of five herbal extracts(FT-5) on insulin resistance, glucose/lipid metabolism, hepatic steatosis, and to investigate whether the combination of FT-5 and pioglitazone would provide a robust effect on diabetes treatment, while may minimize undesirable side-effects of pioglitazone in diabetic Ay gene(KKAy)***: Seven-week-old KKAy mice were randomly divided into five groups: control(CON)group, FT-5(2.0 g/kg) group, pioglitazone(20 mg/kg)(PIO) group, pioglitazone(20 mg/kg) + FT-5(2.0 g/kg)(P + F) group. Age-matched C57 BL/6 J micewere used as the control group. After seven weeks of continuous intragastric administration of medication, the glucose metabolism, insulin sensitivity and lipid metabolism of KKAy mice were evaluated by assessing the fasting blood glucose(FBG), oral glucose tolerance test(OGTT), fasting serum insulin(FINS), insulin tolerance test(ITT), homeostasis model of assessment-insulin resistance index(HOMA-IR), total cholesterol(TC), total triglycerides(TG), and free fatty acids(FFA) in plasma and *** and hepatic adiponectin were measured via enzyme-linked immunosorbent assays. Genes related to adipogenesis and lipolysis in white adipose tissues(WAT) and liver were examined by real-time polymerase chain reaction. Lipid metabolism-related protein expression in the liver of KKAy mice were detected by Western ***: PIO treatment remarkably improved insulin resistance. However, it also showed substantial side effects. FT-5 group exhibited no significant decrease in serum glucose. However, it reduced fasting plasma TG levels and improved hepatic steatosis of KKAy mice. P + F group showed improved insulin resistance and similar body weight gain, as compared with control group. The m RNA expression of genes related to fatty acid oxidation was markedly up-regulated in the liver of P + F *** administration markedly decreased the phosphorylation levels of AMPK, as compared wit

关键词： Pioglitazone Insulin resistance Hepatic steatosis AMP-activated protein kinases FT-5 KKAy mice

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Thermal perception method of virtual chemistry experiments

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Virtual Reality & Intelligent Hardware 2020年第4期2卷 305-315页

作者： Hengwei XU Siru LI Wenpeng SONG Jiajun SUN xinli wu Xiaoqi WANG Wenzhen YANG Zhigeng PAN Abdennour EI RHALIBI Virtual Reality Laboratory Zhejiang Sci-Tech UniversityZhejiang 310018China Hangzhou Normal University Zhejiang 311121China Liverpool John Moores University UK

Background With the aim of addressing the difficulty in identifying temperatures in virtual chemistry experiments,we propose a temperature-sensing simulation method of virtual chemistry *** We construct a virtual chemistry experiment temperature simulation platform,based on which a wearable temperature generation device is *** typical middle school virtual experiments of concentrated sulfuric acid dilution and ammonium nitrate dissolution are conducted to verify the actual effect of the *** The platform is capable to indicate near real-world experimental *** performance of the device not only meets the temperature sensing characteristics of human skin,but also matches the temperature change of virtual chemistry experiments in *** It is demonstrated that this temperature-sensing simulation method can represent exothermic or endothermic chemistry experiments,which is beneficial for students to gain understanding of the principles of thermal energy transformation in chemical reactions,thus avoiding the danger that may be posed in the course of traditional teaching of chemistry experiments *** this method does not have a convenient enough operation for users,the immersion of virtual chemical experiments can be enhanced.

关键词： Virtual reality Chemistry experiment Thermal simulation Temperature feedback

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Fluorescent vital staining of plant sexual cell nuclei with DNA-specific fluorochromes and its application in gametoplast fusion

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Cell Research 1993年第2期3卷 121-130页

作者： YANG HONGYUAN,xinli wu,YONGSHENG MO,CHANG ZHOUDepartment of Biology, wuhan University, wuhan 430072, China Department of Biology Wuhan University Wuhan China

DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to estimate the validity of real vital staining for sexual cell *** were: the cytoplasmic streaming in pollen tubes whose nuclei were stained, the simultaneous visualization of fluorochromatic reaction and nucleus staining in isolated generative cells, and the capability of isolated, prestained generative or sperm cells to fuse with other protoplasts. The results confirmed that 4’,6-diamidino-2-phenylindole (DAPI), Hoechst 33258 and mithramycin could be used as real vital stains, though their efficiency varied from case to case; among them DAPI showed best effect. The fluorescent vital staining technique offered a useful means foridentification and selection of heterokaryons in gametoplas manipulation studies.

关键词： fluorescent vital staining DNA-specific fluorochrome generative cell sperm cell gametoplast fusion.

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Tangnaikang improves insulin resistance and β-cell apoptosis by ameliorating metabolic inflammation in ***-Lepr^(cp)/NDmcr rats

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Journal of Traditional Chinese Medicine 2017年第3期37卷 361-370页

作者： Li Linyi Hisae Yoshitomi Qin Lingling wu xinli Zhou Tian Wang Haiyan Jiang Yueying Wei Ying Xu Tunhai Liu Tonghua Gao Ming Health-cultivation Laboratory of the Ministry of Education Beijing University of Chinese Medicine Key Laboratory of Upper Airway Dysfunction-related Cardiovascular Diseases Beijing Institute of Heart Lung and Blood Vessel Diseases Beijing Anzhen Hospital Capital Medical University School of Pharmaceutical Sciences Mukogawa Women's University Oncology Department Dongfang Hospital Beijing University of Chinese Medicine

OBJECTIVE: To investigate the effects of Tangnaikang(TNK), a mixture of five herbal plant extracts,on inflammation-mediated insulin resistance andβ-cell apoptosis in ***-Leprcp/NDmcr(SHR-cp)***: Seven-week-old SHR-cp rats were randomly divided into a control(CON) group and a TNK(3.24 g/kg) group. Wistar-Kyoto rats at the same age were used as the normal control *** 7 weeks of continuous intragastric administration of TNK, the glucose metabolic status and insulin sensitivity of the rats were evaluated by assessing fasting serum glucose(FBG), the oral glucose tolerance test(OGTT), fasting serum insulin(FINS),and the insulin sensitivity index(ISI). Serum tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), C-reactive protein(CRP) and adiponectin were measured via enzyme-linked immunosorbent *** infiltration and apoptosis in adipose tissues were detected through F4/80 immunohistochemistry and the terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL) assay. Islet morphology and β-cell apoptosis were investigated using double immunofluorescence staining and the TUNEL assay. The expression of cytokine genes in adipose tissue, the liver, and the pancreas was detected in real-time polymerase chain reaction assays. The expression and phosphorylation levels of insulin signaling-, inflammation-, and β-cell survival-related proteins in the liver and pancreas of SHR-cp rats were detected by western ***: TNK(3.24 g/kg) treatment significantly decreased body weight, FBG and FINS; improved impaired glucose tolerance; increased the ISI; reduced serum levels of TNF-α, CRP and IL-6; and increased serum adiponectin. The m RNA expression of inflammatory markers was markedly reduced in the liver, pancreas, and adipose tissue. F4/80-and TUNEL-positive cells in adipose tissues were decreased, as was the number of TUNEL-positiveβ-cells. The phosphorylation of c-Jun N-terminal kinase and that of insulin receptor substrate-1 at seri

关键词： Metabolic inflammation Insulin resis-tance Islets of langerhans Apoptosis Rats, inbredSHR Tangnaikang

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Feasibility and safety of combined interventional procedures in elderly patients with complex cardiovascular diseases:experience of a single medical center

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Journal of Geriatric Cardiology 2005年第3期2卷 188-190页

作者： Caiyi LU Shiwen WANG xinli wu Qiao XUE Taohong HU Muyang YAN Rui CHEN Zhongren ZHAO Haiyun wu Institute of Geriatric Cardiology Chinese PLA General HospitalBeijing 100853China

Objectives:To report the clinical experience of combined interventional procedures in the treatment of elderly patients with coexisting two or more cardiovascular diseases in our medical center,and to assess the feasibility,safety and therapeutic efficacy of this management ***:Patients were selected to the study if:1)age>65 years;2)with coexistence of two or more cardiovascular diseases which are indications for interventional therapy;3)patients'general condition and organ functions allow the performance of combined multiple procedures;4)the predicted procedure time is within 150 min;5)the predicted contrast medium dosage is within 300 *** criteria we analyzed included procedural type,rocedural time,fluoroscopy time,dosage of contrast medium,success rates of the procedures,complications and in-hospital *** patients were followed up for 30.4±9.3 months,to determine the all-cause mortality,recurrence rates and adverse cardiac ***:From January 2000 to December 2004,combined interventional procedures were performed on 136 patients,with 2 procedures on 134 patients and 3 procedures on 2 *** mean procedure time was 115.4±11.6 min,the mean fluoroscopy time was 35.7±9.3 min,and the mean dosage of contrast medium used was 183.6±19.4 *** success rate was 100%,no procedure related death or major complications ***:Performed by a competent team,combined interventional procedures in elderly patients with multiple cardiovascular diseases were feasible and relatively safe.

关键词： cardiovascular disease angioplasty the elderly

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Modified Maze lines plus pulmonary vein isolation created by radiofrequency catheter ablation on the atrial wall to treat atrial fibrillation in elderly

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Journal of Geriatric Cardiology 2005年第2期2卷 95-100页

作者： Caiyi LU Shiwen WANG Xinping DU Yinglong HOU Qiao XUE xinli wu Rui CHEN Peng LIU The Institute of Geriatric Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China The Institute of Geriatric Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China The Institute of Geriatric Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China The Institute of Geriatric Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China The Institute of Geriatric Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China The Institute of Geriatric Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China The Institute of Geriatric Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China The Institute of Geriatric Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China

Objective To evaluate the effect of modified Maze lines plus pulmonary vein (PV) isolation created by radiofrequency catheter ablation (RFCA) on atrial wall guided by a novel geometry mapping system in the treatment of elderly patients with paroxysmal atrial fibrillation (PAF). Methods After regular electrophysiological study, transseptal punctures were achieved twice with Swartz L1 and R1 sheaths. PV angiographies were conducted to evaluate their orifices and branches. A balloon electrode array catheter with 64 electrodes was put in the middle of the left atrium. Atrium geometry was constructed using Ensite 3000 Navx system. Two RFCA lesion loops and three lines (modified Maze) were created on left and right atrial walls. Each lesion point was ablated for 30 seconds with preset temperature 50 (ae) and energy 30W. The disappearance or 80% decrease of the amplitude of target atrial potential and 10 to 20(|), decrease of ablation impedance were used as an index of effective ablation. Results A total of 11 patients (7 male and 4 female, mean age, 68.7±5.1 years) were enrolled. PAF history was 7.9±4.5 years. PAF could not be prevented by mean 3.1±1.6 antiarrhythmic agents in 6.3±3.4 years. None of the patients had complications with structural heart disease or stroke. Left atrial diameter was 41.3±3.6 mm and LVEF was 59.2±3.7% on echocardiography. Two loops and three lines were completed with 67.8±13.1 (73-167) lesion points. Altogether 76-168 (89.4±15.3) lesion points were created in each patient. PAF could not be provoked by rapid burst pacing up to 600 beat per minute delivered from paroxysmal coronary sinus electrode *** PV electrical isolation was confirmed by three-dimensional activation mapping. Mean procedure time was 2.7±0.6 hours and fluoroscopy time was 17.8±9.4 minutes. Patients were discharged with oral aspirin and without antiarrhythmic agents. During follow up of 6.5±1.8 months, seven patients were PAF symptom free (63.6%). PAF attacks were decr

关键词： paroxysmal atrial fibrillation electrophysiology MAZE ablation pulmonary vein ablation

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酸碱刻蚀电沉积钛基HA涂层及其与牛血清白蛋白的相互作用

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稀有金属材料与工程 2008年第2期37卷 357-361页

作者：肖凤娟常虹吴新丽石家庄铁道学院河北石家庄050043 河北师范大学河北石家庄050016

利用酸碱刻蚀两步化学法对Ti进行活化处理,经水热电沉积制备Ti/羟基磷灰石涂层(HA),用SEM,XRD,FTIR和EDS对涂层进行分析和表征,用微控电子万能试验机对基体和涂层的界面结合强度进行测试,将涂层在牛血清白蛋白溶液(BSA)中浸泡2h^3d后,... 详细信息

利用酸碱刻蚀两步化学法对Ti进行活化处理,经水热电沉积制备Ti/羟基磷灰石涂层(HA),用SEM,XRD,FTIR和EDS对涂层进行分析和表征,用微控电子万能试验机对基体和涂层的界面结合强度进行测试,将涂层在牛血清白蛋白溶液(BSA)中浸泡2h^3d后,分析涂层与BSA溶液的FTIR和XSP光谱。结果表明:活化处理后的Ti表面形成了活性TiO2过渡层,活化过渡层使涂层与基体的结合强度显著提高;涂层形貌、元素比例与骨组织纤维相一致。涂层与BSA作用后的FTIR光谱表明,在涂层中引入了BSA酰胺带基团,在BSA溶液中又出现HA中PO43-基团,蛋白质酰胺I带、酰胺II带和HA中PO43-的红外光谱的位置和谱峰均发生变化,由此推测了Ti/HA涂层与BSA间的作用机制,指出:正是由于Ti/HA涂层与BSA间的相互作用使涂层得以在蛋白质结构中交互有序地排列生长,这是Ti/HA涂层具有生物相容性的基础。

关键词：钛活化处理羟基磷灰石电沉积

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包钢总排污水处理中心沉淀处理工艺工程实例

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工业水处理 2010年第4期30卷 90-92页

作者：吴新力孔军田小红包钢设计研究院内蒙古包头014010

通过对包钢总排污水处理工艺流程中沉淀处理工艺的初步理论分析,并结合工程设计,对机械加速沉淀工艺进行了介绍,给出了设计进出水水质、相关工艺参数及应用情况。

关键词：污水处理:沉淀处理:絮凝

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Long-term effect of stenting in unprotected left main coronary artery disease in the elderly

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Journal of Geriatric Cardiology 2005年第4期2卷 218-222页

作者： Caiyi LU Shiwen WANG Lingling LIU Qiao XUE xinli wu Taohong HU Pingshuan DONG Zhiping WANG Shenfang TIAN Pinfa LIU Jicai ZANG Institute of Geriatric Cardiology Chinese PLA General HospitalBeijing 100853China Affiliated Hospital of Henan Science and Technology University Affliated Hospital of Changzhi Medical College Qingdao First Hospital Qinghai Cardiovascular Hospital Baoding Second Hospital

Objective To evaluate the feasibility,safety and efficacy of percutaneous stent implantation for treating left main coronary artery(LMCA)*** Consecutive patients with unprotected left main coronary artery disease treated by stent-based percutaneous intervention(PCI)at 6 medical centers in China were *** data and clinical outcomes were obtained from all *** From January 2001 to December 2004,138 patients(79 males and 59 females;mean age:69.7±5.8 years)underwent PCI for LMCA *** metal stents(BMS)were implanted in 51 patients with non-bifurcational lesions and in 5 patients with bifurcational lesions from January of 2001 to June of 2003(BMS group);.Drug eluting stents(DES)were used unselectively to cover both bifurcational and non-bifurcational lesions in 86 patients from July of 2003 to December of 2004(DES group).Procedural success rate of the 138 cases was 98%(135/138).One patient(0.7%)with bifurcation lesion who was treated with DES died from severe heart failure 2 weeks after the *** a mean follow up period of 21.3±5.6 months,one patient died from renal failure,one from sudden cardiac death,4 underwent target lesion revascularization(TLR)in the BMS group,which all occurred in patients with bifurcational lesions;whereas in the DES group no deaths occurred and only one patient with bifurcational lesion had ***(1)PCI is feasible and relatively safe to treat unprotected left main coronary artery disease in elderly patients at medical centers with experienced professionals.(2)BMS and DES have similar immediate and long-term efficacy in the treatment of ostium and shaft lesions of the LMCA.(3)DES are strongly suggested in the therapy of distal bifurcation lesion of unprotected LMCA.

关键词： coronary artery disease interventional therapy angioplasty,stent

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阅读是一项国家战略——国外政府推动全民阅读措施简述

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科教导刊 2011年第11期 18-19页

作者：吴新莉空军雷达学院湖北武汉430019

阅读可以改变人的文化认同、振奋民族精神、凝聚民心、提升国家竞争力。可以说,国民阅读能力的高低直接影响到一个国家和民族的未来。在中国将全民阅读作为一项国家战略是大势所趋。在此,本文介绍了几个阅读大国实施的国民阅读促进措施... 详细信息

阅读可以改变人的文化认同、振奋民族精神、凝聚民心、提升国家竞争力。可以说,国民阅读能力的高低直接影响到一个国家和民族的未来。在中国将全民阅读作为一项国家战略是大势所趋。在此,本文介绍了几个阅读大国实施的国民阅读促进措施,希望能有所借鉴。

关键词：国家策略全民阅读措施

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