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GLUL stabilizes N-Cadherin by antagonizing β-Catenin to inhibit the progresses of gastric cancer

Acta Pharmaceutica Sinica B 2024年第2期14卷 698-711页

作者： Qiwei Jiang Yong Li Songwang Cai xingyuan shi Yang Yang Zihao Xing Zhenjie He Shengte Wang Yubin Su Meiwan Chen Zhesheng Chen Zhi shi Department of Cell Biology&Institute of Biomedicine National Engineering Research Center of Genetic MedicineState Key Laboratory of Bioactive Molecules and Druggability AssessmentMOE Key Laboratory of Tumor Molecular BiologyGuangdong Provincial Key Laboratory of Bioengineering MedicineCollege of Life Science and TechnologyJinan UniversityGuangzhou 510632China Department of Gastrointestinal Surgery&General Surgery the Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Department of Thoracic Surgery The First Affiliated Hospital of Jinan UniversityGuangzhou 510632China Department of Radiation Oncology The Fifth Hospital of Guangzhou Medical UniversityGuangzhou 510150China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao 519000China Department of Pharmaceutical Sciences College of Pharmacy and Health SciencesSt.John’s UniversityQueensNY 11439USA

Glutamate-ammonia ligase(GLUL, also known as glutamine synthetase) is a crucial enzyme that catalyzes ammonium and glutamate into glutamine in the ATP-dependent condensation. Although GLUL plays a critical role in multiple cancers, the expression and function of GLUL in gastric cancer remain unclear. In the present study, we have found that the expression level of GLUL was significantly lower in gastric cancer tissues compared with adjacent normal tissues, and correlated with N stage and TNM stage, and low GLUL expression predicted poor survival for gastric cancer patients. Knockdown of GLUL promoted the growth, migration, invasion and metastasis of gastric cancer cells in vitro and in vivo, and vice versa, which was independent of its enzyme activity. Mechanistically, GLUL competed with β-Catenin to bind to N-Cadherin, increased the stability of N-Cadherin and decreased the stability of β-Catenin by alerting their ubiquitination. Furthermore, there were lower N-Cadherin and higher β-Catenin expression levels in gastric cancer tissues compared with adjacent normal tissues. GLUL protein expression was correlated with that of N-Cadherin, and could be the independent prognostic factor in gastric cancer. Our findings reveal that GLUL stabilizes N-Cadherin by antagonizing β-Catenin to inhibit the progress of gastric cancer.

关键词： Gastric cancer GLUL N-Cadherin β-Catenin Enzyme

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全方位护理干预对肾衰竭血液透析患者影响研究

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社区医学杂志 2024年第9期22卷 306-311页

作者：刘燕燕董永清张松石兴元河南大学淮河医院血液透析室河南开封475000

目的探讨基于营养、心理、运动的全方位护理干预对肾衰竭血液透析患者营养状况、心理状态、生存质量及并发症的影响。方法选取河南大学淮河医院2021-01-01-2022-07-31收治的肾衰竭患者106例作为研究对象,随机数字表法分为传统护理组和... 详细信息

目的探讨基于营养、心理、运动的全方位护理干预对肾衰竭血液透析患者营养状况、心理状态、生存质量及并发症的影响。方法选取河南大学淮河医院2021-01-01-2022-07-31收治的肾衰竭患者106例作为研究对象,随机数字表法分为传统护理组和全方位护理组,各53例。2组患者均接受血液透析治疗,传统护理组采用传统护理干预,全方位护理组在传统护理干预基础上进行基于营养、心理、运动的全方位护理干预。比较2组患者营养状况〔血清白蛋白(ALB)、血红蛋白(Hb)、前白蛋白(PA)〕、心理状态〔抑郁自评量表(SDS)、焦虑自评量表(SAS)〕、健康行为〔健康促进生活方式量表(HPLP-Ⅱ)〕、自我效能〔自我效能感量表(GSES)〕、生存质量〔肾脏疾病生存质量简表(KDQOL-SFTM)〕、治疗依从性及并发症。结果护理干预3个月后,2组患者ALB、Hb、PA水平均高于干预前,且全方位护理组血清ALB水平为(43.18±3.89)g/L,高于传统护理组的(38.42±3.77)g/L,F=21.417,P<0.001;Hb水平为(115.74±5.62)g/L,高于传统护理组的(101.33±5.44)g/L,F=89.945,P<0.001;PA水平为(310.36±7.38)mg/L,高于传统护理组的(280.43±7.04)mg/L,F=230.371,P<0.001。干预3个月后,2组患者SDS、SAS、KDQOL-SFTM评分均低于干预前,且全方位护理组SDS评分为(48.32±3.48)分,低于传统护理组的(52.45±3.37)分,F=19.090,P<0.001;SAS评分为(49.25±3.27)分,低于传统护理组的(51.85±3.35)分,F=8.914,P<0.001;KDQOL-SFTM评分为(49.72±4.89)分,低于传统护理组的(55.38±4.75)分,F=20.423,P<0.001。HPLP-Ⅱ和GSES评分均高于干预前,且全方位护理组HPLP-Ⅱ评分为(136.47±6.94)分,高于传统护理组的(118.45±7.35)分,F=89.387,P<0.001;GSES评分为(30.51±2.87)分,高于传统护理组的(26.75±2.94)分,F=21.893,P<0.001。全方位护理组治疗依从性(96.23%)高于传统护理组(83.02%),差异有统计学意义,χ^(2)=4.970,P=0.026;并发症发生率(7.55%)低于传统护理组(22.64%),差异有统计学意义,χ^(2)=4.711,P=0.030。结论基于营养、心理、运动的全方位护理干预能改善肾衰竭血液透析患者的营养状况和负性心理状态,提高自我效能及治疗依从性,建立健康行为,降低并发症发生的风险,有利于提高生存质量。

关键词：肾衰竭血液透析白蛋白血红蛋白前白蛋白抑郁焦虑自我效能

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KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer

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Acta Pharmaceutica Sinica B 2023年第10期13卷 4217-4233页

作者： Cancan Zheng Xiaomei Yu Taoyang Xu Zhichao Liu Zhili Jiang Jiaojiao Xu Jing Yang Guogeng Zhang Yan He Han Yang xingyuan shi Zhigang Li Jinbao Liu Wen Wen Xu Key Laboratory of Biological Targeting Diagnosis Therapy and Rehabilitation of Guangdong Higher Education Institutesthe Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510230China Department of Thoracic Surgery Shanghai Chest HospitalSchool of MedicineShanghai Jiao Tong University.Shanghai 200030China Department of Radiation Oncology the Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510230China Department of Thoracic Surgery Sun Yat-sen University Cancer CenterGuangzhou 510060China Guangdong Provincial Key Laboratory of Protein Modification and Degradation State Key Laboratory of Respiratory Diseaseand School of Basic Medical SciencesGuangzhou Medical UniversityGuangzhou 511495China

Increasing evidences suggest the important role of calcium homeostasis in hallmarks of cancer,but its function and regulatory network in metastasis remain unclear.A comprehensive investigation of key regulators in cancer metastasis is urgently *** sequencing(RNA-seq)of primary esophageal squamous cell carcinoma(ESCC)and matched metastatic tissues and a series of gain/loss-of-function experiments identified potassium channel tetramerization domain containing 4(KCTD4)as a driver of cancer ***4 expression was found upregulated in metastatic *** KCTD4 expression is associated with poor prognosis in patients with ESCC and contributes to cancer metastasis in vitro and in ***,KCTD4 binds to CLIC1 and disrupts its dimerization,thus increasing intracellular Ca^(2+)level to enhance NFATc1-dependent fibronectin ***4-induced fibronectin secretion activates fibroblasts in a paracrine manner,which in turn promotes cancer cell invasion via MMP24 signaling as positive ***,a lead compound K279-0738 significantly suppresses cancer metastasis by targeting the KCTD4-CLIC1 interaction,providing a potential therapeutic *** together,our study not only uncovers KCTD4 as a regulator of calcium homeostasis,but also reveals KCTD4/CLIC1-Ca^(2+)-NFATc1-fibronectin signaling as a novel mechanism of cancer *** findings validate KCTD4 as a potential prognostic biomarker and therapeutic target for ESCC.

关键词： Cancermetastasis Calcium homeostasis NFAT signaling Fibroblasts Esophageal cancer

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Polyphyllin Ⅰ enhances tumor necrosis factor-related apoptosis-inducing ligand-induced inhibition of human osteosarcoma cell growth via downregulating the Wnt/β-catenin pathway

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Journal of Traditional Chinese Medicine 2024年第2期44卷 251-259页

作者： CHANG Junli ZHAO Fulai SUN xingyuan MA Xiaoping ZHAO Peng ZHOU Chujie shi Binhao GU Wenchao WANG Yongjun YANG Yanping Spine Disease Institute Longhua Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 200032China Department of Diagnostic and Interventional Radiology University of TsukubaIbaraki 315-0114Japan

OBJECTIVE:To investigate the synergistic effects of polyphyllin Ⅰ(PPⅠ)combined with tumor necrosis factorrelated apoptosis-inducing ligand(TRAIL)on the growth of osteosarcoma cells through downregulating the Wnt/β-catenin signaling ***:Cell viability,apoptosis and cell cycle distribution were examined using cell counting kit-8 and flow cytometry *** morphology of cancer cells was observed with inverted phase contrast *** migration and invasion abilities were examined by xCELLigence real time cell analysis DP system and transwell *** expressions of poly(adenosine diphosphate-ribose)polymerase,C-Myc,Cyclin B1,cyclin-dependent kinases 1,N-cadherin,Vimentin,Active-β-catenin,β-catenin,p-glycogen synthase kinase 3β(GSK-3β)and GSK-3βwere determined by Western blotting ***:PPⅠ sensitized TRAIL-induced decrease of viability,migration and invasion,as well as increase of apoptosis and cell cycle arrest of MG-63 and U-2 OS osteosarcoma *** synergistic effect of PPⅠwith TRAIL in inhibiting the growth of osteosarcoma cells was at least partially realized through the inactivation of Wnt/β-catenin signaling ***:The combination of PPⅠ and TRAIL is potentially a novel treatment strategy of osteosarcoma.

关键词： osteosarcoma tumor necrosis factor-related apoptosis-inducing ligand Wnt signaling pathway beta-catenin polyphyllinⅠ

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SRS MapCHECK与EDOSE在立体定向放疗计划剂量验证中的对比

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中国医学物理学杂志 2022年第1期39卷 23-27页

作者：袁玲刘颖徐瑶瑶张红利张晓鹏王鑫石兴源广州医科大学附属第五医院放疗科广东广州510700

目的:使用SRS MapCHECK和EDOSE对立体定向放疗(SRT)计划进行剂量验证,并比较它们的验证结果。方法:首先比较两种系统在不同方野(2 cm×2 cm、4 cm×4 cm、6 cm×6 cm、10 cm×10 cm)的γ通过率(3%/3 mm),然后选择29例SRT计划,分析两种... 详细信息

目的:使用SRS MapCHECK和EDOSE对立体定向放疗(SRT)计划进行剂量验证,并比较它们的验证结果。方法:首先比较两种系统在不同方野(2 cm×2 cm、4 cm×4 cm、6 cm×6 cm、10 cm×10 cm)的γ通过率(3%/3 mm),然后选择29例SRT计划,分析两种系统在不同γ标准下的绝对剂量通过率,最后分析EDOSE建立的4种不同物理模型对SRT计划剂量验证的影响。结果:SRS MapCHECK在所有方野和29例SRT计划的绝对剂量γ通过率均高于EDOSE,两者的验证结果有统计学差异(P<0.05);SRS MapCHECK及EDOSE在29例SRT计划的γ通过率(2%/2 mm)分别是为98.60%±2.14%和96.53%±2.41%。基于EDOSE的不同物理模型验证结果有统计学差异,平均γ通过率(2%/2 mm)偏差为1.8%~5.1%。结论:SRS MapCHECK和EDOSE系统均满足SRT计划剂量验证的要求,且SRS MapCHECK的剂量验证通过率优于EDOSE;不同的EDOSE物理模型对SRT计划剂量验证有影响。

关键词：立体定向放疗 SRS MapCHECK EDOSE γ通过率剂量验证

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MiR-199a-3p/5p靶向DUSP5/MAP3K11调控翼状胬肉成纤维细胞纤维化和迁移

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武汉大学学报（医学版） 2024年第3期45卷 272-278页

作者：朱阁王陈吴细英石洋郑芳袁玥琪燕星元严明武汉大学中南医院眼科湖北武汉430071 武汉大学中南医院检验科/基因诊断中心湖北武汉430071 武汉爱尔眼科汉口医院湖北武汉430021

目的:探讨miR-199a-3p/5p及其靶基因DUSP5/MAP3K11在人翼状胬肉成纤维细胞(HPF)中的作用。方法:采用外植体方法从手术切除的翼状胬肉组织中培养原代HPF,实时定量聚合酶链反应(qRT-PCR)验证其表型。MiR-199a-3p/5p模拟物和抑制剂转染HPF... 详细信息

目的:探讨miR-199a-3p/5p及其靶基因DUSP5/MAP3K11在人翼状胬肉成纤维细胞(HPF)中的作用。方法:采用外植体方法从手术切除的翼状胬肉组织中培养原代HPF,实时定量聚合酶链反应(qRT-PCR)验证其表型。MiR-199a-3p/5p模拟物和抑制剂转染HPF。细胞划痕试验、Transwell法测定细胞迁移能力。免疫蛋白印记分析(Western Blot)和qRT-PCR检测靶基因DUSP5/MAP3K11和纤维化标志物的变化。免疫荧光法验证上皮-间质转化(EMT)相关蛋白的胞内定位。结果:与人结膜成纤维细胞相比,HPF的EMT和纤维化表型显著升高。MiRNA-199a-3p/5p模拟物下调DUSP5/MAP3K11,并促进HPF纤维化和EMT,miR-199a-3p/5p抑制剂上调DUSP5/MAP3K11,抑制HPF纤维化、EMT及迁移。结论:MiRNA-199a-3p/5p可能靶向DUSP5/MAP3K11,以调控翼状胬肉成纤维细胞的纤维化、EMT和迁移能力。

关键词：翼状胬肉纤维化再上皮化上皮-间质转化 miR-199a 迁移

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摩托车气阀门材料进展和热处理新发展

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摩托车技术 2021年第11期 41-45页

作者：史兴原张先鸣苏州工品一号网络科技有限公司南京福贝尔五金制品有限公司

气阀门是摩托车发动机的重要零件之一,按摩托车气阀门材料市场的最新发展和需求,共有15个钢号。目前,摩托车气阀门选择马氏体型钢、奥氏体型钢和高温合金材料。气门的热处理工艺,马氏体钢气阀门需调质处理,奥氏体钢气阀门根据产品图样,... 详细信息

气阀门是摩托车发动机的重要零件之一,按摩托车气阀门材料市场的最新发展和需求,共有15个钢号。目前,摩托车气阀门选择马氏体型钢、奥氏体型钢和高温合金材料。气门的热处理工艺,马氏体钢气阀门需调质处理,奥氏体钢气阀门根据产品图样,分为固溶、固溶和时效处理,高温合金固溶+时效处理。

关键词：摩托车气阀门材料马氏体型钢奥氏体型钢高温合金热处理

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放射性肺损伤过程中细胞死亡机制探讨

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中华生物医学工程杂志 2023年第3期29卷 351-355页

作者：石兴源陈婷梁阐锦聂豪唐斯晗江志力广州医科大学附属第五医院放疗科广州510700

放射治疗是胸部肿瘤的主要治疗方法之一,在治疗过程中,放射性肺损伤是最常见且最严重的并发症之一。目前关于放射性肺损伤产生的原因尚未明确,尚无有效的治疗手段。放射性肺损伤成为了胸部肿瘤放射治疗中最重要的剂量限制因素和亟待解... 详细信息

放射治疗是胸部肿瘤的主要治疗方法之一,在治疗过程中,放射性肺损伤是最常见且最严重的并发症之一。目前关于放射性肺损伤产生的原因尚未明确,尚无有效的治疗手段。放射性肺损伤成为了胸部肿瘤放射治疗中最重要的剂量限制因素和亟待解决的重要问题。研究放射性肺损伤过程中不同的细胞死亡机制,有利于为临床实践提供新的思路。现就放射性肺损伤中,如凋亡、自噬、焦亡、铁死亡等不同的细胞死亡方式研究进展进行综述.

关键词：放射性肺损伤凋亡自噬焦亡铁死亡

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Terahertz bound states in the continuum with incident angle robustness induced by a dual period metagrating

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Photonics Research 2022年第3期10卷 810-819页

作者： Wenqiao shi Jianqiang Gu xingyuan Zhang Quan Xu Jiaguang Han Quanlong Yang Longqing Cong Weili Zhang Center for Terahertz Waves and College of Precision Instrument and Optoelectronics Engineering Tianjin Universityand Key Laboratory of Optoelectronics Information and TechnologyMinistry of EducationTianjin 300072China Nonlinear Physics Centre Australian National UniversityCanberraACT 2601Australia Department of Electrical and Electronic Engineering Southern University of Science and TechnologyShenzhen 518055China School of Electrical and Computer Engineering Oklahoma State UniversityStillwaterOklahoma 74078USA

Metasurface-empowered bound state in the continuum(BIC)provides a unique route for fascinating functional devices with infinitely high quality *** method is particularly attractive to the terahertz community because it may essentially solve the deficiencies in terahertz filters,sensors,lasers,and nonlinear ***,most BIC metasurfaces are limited to specified incident angles that seriously dim their application ***,we propose that a dual-period dielectric metagrating can support multiple families of BICs that originate from guided mode resonances in the dielectric grating and exhibit infinite quality factors at arbitrarily tilted *** robustness was analyzed based on the Bloch theory and verified at tilted incident *** also demonstrate that inducing geometric asymmetry is an efficient way to manipulate the leakage and coupling of these BICs,which can mimic the electromagnetically induced transparency(EIT)effect in our dual-period *** this demonstration,a slow-light effect with a measured group delay of 117 ps was *** incidence-insensitive BICs proposed here may greatly extend the application scenarios of the BIC *** high Q factor and outstanding slow-light effect in the metagrating show exciting prospects in realizing high-performance filters,sensors,and modulators for prompting terahertz applications.

关键词： incident dielectric continuum

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Study on the Grey Comprehensive Evaluation of Kansei Image for Product Design（ID:H-009）

Study on the Grey Comprehensive Evaluation of Kansei Image f...

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第十四届工业工程与工程管理国际会议

作者： ~1Yang Xu ~2Wang xingyuan ~2shi Yan 1 Shandong Jianzhu University Jin’an P.R.China 250014 2 Shandong University Jin’an P.R.China 250100

＜正＞By the image quantitative evaluation of the popular products and using clustering analysis,evaluators select the typical samples as the evaluation schemes of product *** the basis of statistical analysis of the evaluation in- formation,the product Kansei image is described as the interval numbers and the multi-objective information matrix of the evaluation schemes is constructed.A new evaluation method for product exterior design based on the grey image in- tervals is presented based on the grey relational decision-making *** method can sort the product design,and by way of the theoretical basis,it intensifies designers’ understanding of the customer’s preference degree and demands accurately.A practical example is given to illustrate the use of the proposed method.

关键词： Kansei Image Grey Relational Decision-Making Product Design

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