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Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing

Bioactive Materials 2022年第11期7卷 178-194页

作者： Yuzhen Wang xingyu yuan Bin Yao Shuoji Zhu Ping Zhu Sha Huang Guangdong Cardiovascular Institute Guangdong Provincial People’s HospitalGuangdong Academy of Medical Sciences106 Zhongshan Er RoadGuangzhouGuangdong510080PR China Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Department Chinese PLA General Hospital28 Fu Xing RoadBeijing100853PR China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and RegenerationChinese PLA General Hospital and PLA Medical College51 Fu Cheng RoadBeijing100048PR China Department of Burn and Plastic Surgery Air Force Hospital of Chinese PLA Central Theater Command589 Yunzhong RoadPingcheng DistrictDatongShanxi037006PR China School of Medicine Nankai University94 Wei Jing RoadTianjin300071PR China Academy of Medical Engineering and Translational Medicine Tianjin University300072PR China

Extrusion-based bioprinting (EBB) holds potential for regenerative medicine. However, the widely-used bioinks of EBB exhibit some limitations for skin regeneration, such as unsatisfactory bio-physical (i.e., mechanical, structural, biodegradable) properties and compromised cellular compatibilities, and the EBB-based bioinks with therapeutic effects targeting cutaneous wounds still remain largely undiscussed. In this review, the printability considerations for skin bioprinting were discussed. Then, current strategies for improving the physical properties of bioinks and for reinforcing bioinks in EBB approaches were introduced, respectively. Notably, we highlighted the applications and effects of current EBB-based bioinks on wound healing, wound scar formation, vasculari-zation and the regeneration of skin appendages (i.e., sweat glands and hair follicles) and discussed the challenges and future perspectives. This review aims to provide an overall view of the applications, challenges and promising solutions about the EBB-based bioinks for cutaneous wound healing and skin regeneration.

关键词： Bioink Extrusion-based bioprinting Cutaneous wound healing Skin regeneration

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The role of CTHRC1 in promotion of cutaneous wound healing

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Signal Transduction and Targeted Therapy 2022年第7期7卷 2144-2147页

作者： Xianlan Duan xingyu yuan Bin Yao Wei Song Zhao Li Enhejirigala Yi Kong Yuzhen Wang Xiaobing Fu Sha Huang School of Medicine NanKai University94 Wei Jin RoadTianjin 300071People’s Republic of China Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Department PLA General Hospital51 Fu Cheng RoadBeijing 100048People’s Republic of China Academy of Medical Engineering and Translational Medicine Tianjin University92 Weijin RoadTianjin 300072People’s Republic of China

Dear Editor,Wound healing after tissue injury is a well-orchestrated process involving various cell types,growth factors,and extracellular matrix(ECM)components.Collagen triple helix repeat containing1(CTHRC1),a secreted ECM protein,is transiently expressed in the repair process after arterial injury and myocardial infarction,1 regulating collagen matrix deposition and cell migration through TGF-βsignaling pathway.2 However,the expression and function of CTHRC1 during cutaneous wound repair remain largely undefined.We initially investigated the spatiotemporal expression of CTHRC1 during full-thickness cutaneous wound healing.By searching the GEO database,we found a transcriptome dataset(GSE23006),which analyzed the gene expression of mouse skin wounds from day 0 to day 10,spanned all stages of the wound healing process.

关键词： healing wound arterial

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Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction in vivo

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Burns & Trauma 2022年第1期10卷 387-400页

作者： xingyu yuan Xianlan Duan Zhao Li Bin Yao Enhejirigala Wei Song Yi Kong Yuzhen Wang Fanliang Zhang Liting Liang Shijun Zhu Mengde Zhang Chao Zhang Sha Huang Xiaobing Fu School of Medicine Nankai University94 Wei Jin RoadTianjin 300071PR China Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Department PLA General Hospital28 Fu Xing RoadBeijing 100853PR China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and RegenerationChinese PLA General Hospital and PLA Medical College51 Fu Cheng RoadBeijing 100048PR China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048PR China Academy of Medical Engineering and Translational Medicine Tianjin University92 Weijin RoadTianjin300072PR China College of Graduate Tianjin Medical UniversityTianjin 300070PR China Institute of Basic Medical Research Inner Mongolia Medical UniversityHohhot 010110Inner MongoliaPR China Department of Burn and Plastic Surgery Air Force Hospital of Chinese PLA Central Theater CommandDatong 037000ShanxiPR China

Background:Sweat glands(SGs)have low regenerative potential after severe burns or trauma and their regeneration or functional recovery still faces many obstacles.In practice,restoring SG function requires not only the structural integrity of the gland itself,but also its neighboring tissues,especially blood vessels.Collagen triple helix repeat containing-1(CTHRC1)was first identified in vascular repair,and increasing reports showed a close correlation between cutaneous appendage specification,patterning and regeneration.The purpose of the present study was to clarify the role of CTHRC1 in SGs and their adjacent microvessels and find therapeutic strategies to restore SG function.Methods:The SGs and their adjacent microvascular network of Cthrc^(1−/−)mice were first inves-tigated using sweat test,laser Doppler imaging,tissue clearing technique and transcriptome analysis.The effects of CTHRC1 on dermal microvascular endothelial cells(DMECs)were further explored with cell proliferation,DiI-labeled acetylated low-density lipoprotein uptake,tube for-mation and intercellular junction establishment assays.The effects of CTHRC1 on SG function restoration were finally confirmed by replenishing the protein into the paws of Cthrc(1−/−)mice.Results:CTHRC1 is a key regulator of SG function in mice.At the tissue level,Cthrc1 deletion resulted in the disorder and reduction of the microvascular network around SGs.At the molecular level,the knockout of Cthrc1 reduced the expression of vascular development genes and functional proteins in the dermal tissues.Furthermore,CTHRC1 administration considerably enhanced SG function by inducing adjacent vascular network reconstruction.Conclusions:CTHRC1 promotes the development,morphogenesis and function execution of SGs and their neighboring vasculature.Our study provides a novel target for the restoration or regeneration of SG function in vivo.

关键词： Collagen triple helix repeat containing-1 Sweat glands Microvascular network Dermalmicrovascular endothelial cells Reconstruction

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Reciprocal interaction between vascular niche and sweat gland promotes sweat gland regeneration

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Bioactive Materials 2023年第3期21卷 340-357页

作者： xingyu yuan Xianlan Duan Enhejirigala Zhao Li Bin Yao Wei Song Yuzhen Wang Yi Kong Shijun Zhu Fanliang Zhang Liting Liang Mengde Zhang Chao Zhang Deling Kong Meifeng Zhu Sha Huang Xiaobing Fu School of Medicine Nankai University94 Weijin RoadTianjin300071PR China Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Department PLA General Hospital28 Fuxing RoadBeijing100853PR China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and RegenerationChinese PLA General Hospital and PLA Medical College51 Fucheng RoadBeijing100048PR China College of Graduate Tianjin Medical UniversityTianjin300070PR China Institute of Basic Medical Research Inner Mongolia Medical UniversityHohhot010110Inner MongoliaPR China Academy of Medical Engineering and Translational Medicine Tianjin University92 Weijin RoadTianjin300072PR China Department of Burn and Plastic Surgery Air Force Hospital of Chinese PLA Central Theater CommandDatong037000ShanxiPR China College of Life Science State Key Laboratory of Medicinal Chemical BiologyKey Laboratory of Bioactive Materials of Ministry of EducationNankai University94 Weijin RoadTianjin300071PR China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing100048PR China

The incorporation of vasculature is known to be effective in tissue or organ functional regeneration.However,a vague understanding of the interaction between epidermal appendages and their vascular niches is a foremost obstacle to obtaining sweat gland(SG)-specific vasculature units.Here,we map their precise anatomical con-nections and report that the interplay between SG cells(SGCs)and the surrounding vascular niche is key for glandular development and homeostasis maintenance.To replicate this interplay in vitro,we used three-dimensional(3D)bioprinting to generate reproducible SGC spheroids from differentiated adipose-derived mesenchymal stem cells(ADSCs).With dermal microvascular endothelial cells(DMECs),sacrificial templates made from poly(ε-caprolactone)(PCL)were fabricated to pattern the vascular niche.This interplay model promoted physiologically relevant vascularized glandular morphogenesis in vitro and in vivo.We identified a reciprocal regulatory mechanism for promoting SGs regeneration via contact-independent cell communication and direct cell-cell interactions between SGs and the vasculature.We envision the successful use of our approach for vascularized organ regeneration in the near future.

关键词： Vascular niche 3D bioprinting Sweat gland-vasculature interaction model ECM scaffold Sweat gland regeneration

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Zircon U-Pb Ages and Hf Isotopes of Neoproterozoic Meta-Igneous Rocks in the Liansandao Area,Northern Sulu Orogen,Eastern China,and the Tectonic Implications

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Journal of Earth Science 2019年第6期30卷 1230-1242页

作者： Zhaojun Song Huimin Liu Fanxue Meng xingyu yuan Qiao Feng Dingwu Zhou Juan Ramon Vidal Romani Hongbo Yan Shandong Key Laboratory of Depositional Mineralization and Sedimentary Minerals Shandong University of Science and TechnologyQingdao 266590China Laboratory for Marine Geology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266061China Instituto Universitario de Geologia(Universidad de A Coruna) Campus Universitario de Elvina15071 CorunaSpain

The Sulu Orogen preserves the Neoproterozoic tectonic-magmatic events,corresponding to the breaking up of the Rodinia supercontinent.The ages and petrogenesis of meta-igneous rocks in the Liansandao area in the northern Sulu Orogen are not well-constrained.This study reports zircon U-Pb ages and Hf isotopes of these rocks from the Liansandao area.Three meta-igneous rock samples give similar weighted mean 206 Pb/238 U ages of 744±11,767±12,and 762±15 Ma,respectively,indicating the Neoproterozoic crystallization ages.These rocks formed coevally with the Wulian and Yangkou intrusions that located along the Yantai-Qingdao-Wulian fault zone.The Neoproterozoic ages indicate that the meta-igneous rocks from the Liansandao area have affinity to the Yangtze Block.The three samples haveεHf(t)values of-7.2–-10.5,-6.0–-17.5,and-6.8–-12.0,respectively.These negativeεHf(t)values indicate a primarily crustal source.However,the widely variousεHf(t)values that are higher than the continental crust,suggesting magma mixing between mantle-derived materials and the continental crust or source heterogeneity.Combined with the Hf model ages and geochemical characteristics,the monzodiorite(sample LSD-2)is most likely to be mantle-derived magma then interacted with ancient continental crust,and the granitic protolith(samples LSD-1 and LSD-3)in the Liansandao area might derive from the re-melting of a Paleoproterozoic continental crust at^750 Ma,resulting from the upwelling and underplating of mantle-derived magma formed in an extensional setting due to the break-up of the Rodinia supercontinent.

关键词： Neoproterozoic zircon U-Pb age zircon Lu-Hf isotope Sulu Orogen

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Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles

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Burns & Trauma 2021年第1期9卷 475-487页

作者： Yijie Zhang Enhejirigala Bin Yao Zhao Li Wei Song Jianjun Li Dongzhen Zhu Yuzhen Wang Xianlan Duan xingyu yuan Sha Huang Xiaobing Fu Research Center for Tissue Repair and Regeneration Medical Innovation Research Department and the Fourth Medical CenterChinese PLA General Hospital and PLA Medical CollegeBeijing 100048China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and RegenerationChinese PLA General Hospital and PLA Medical CollegeBeijing 100853China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048China College of Graduate Tianjin Medical UniversityTianjin 300070China Institute of Basic Medical Research Inner Mongolia Medical UniversityHohhot 010110Inner MongoliaChina The Shenzhen Key Laboratory of Health Sciences and Technology Graduate School at ShenzhenTsinghua UniversityShenzhen 518055GuangdongChina Department of General Surgery the Sixth Medical CenterChinese PLA General HospitalBeijing 100048China Department of Burn and Plastic Surgery Air Force Hospital of Chinese PLA Central Theater CommandDatong 037000ShanxiChina School of Medicine Nankai UniversityTianjin 300071China

Background:Sweat glands(SGs)and hair follicles(HFs)are two important cutaneous appendages that play crucial roles in homeostatic maintenance and thermoregulation,and their interaction is involved in wound healing.SGs can be regenerated from mesenchymal stem cell-laden 3D bioprinted scaffolds,based on our previous studies,whereas regeneration of HFs could not be achieved in the same model.Due to the lack of an in vitro model,the underlying molecular mechanism of the interaction between SGs and HFs in regeneration could not be fully understood.The purpose of the present study was to establish an in vitro model of skin constructs with SGs and HFs and explore the interaction between these two appendages in regeneration.Methods:To investigate the interaction effects between SGs and HFs during their regeneration processes,a combined model was created by seeding HF spheroids on 3D printed SG scaffolds.The interaction between SG scaffolds and HF spheroids was detected using RNA expression and immunofluorescence staining.The effects of microenvironmental cues on SG and HF regeneration were analysed by altering seed cell types and plantar dermis homogenate in the scaffold.Results:According to this model,we overcame the difficulties in simultaneously inducing SG and HF regeneration and explored the interaction effects between SG scaffolds and HF spheroids.Surprisingly,HF spheroids promoted both SG and HF differentiation in SG scaffolds,while SG scaffolds promoted SG differentiation but had little effect on HF potency in HF spheroids.Specifically,microenvironmental factors(plantar dermis homogenate)in SG scaffolds effectively promoted SG and HF genesis in HF spheroids,no matter what the seed cell type in SG scaffolds was,and the promotion effects were persistent.Conclusions:Our approach elucidated a new model for SG and HF formation in vitro and provided an applicable platform to investigate the interaction between SGs and HFs in vitro.This platform might facilitate 3D skin constructs with multiple appendages and unveil the

关键词： Skin regeneration Sweat glands Hair follicle 3D bioprinting Spheroid culture Skin constructs

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河流作用下堵江滑坡运动特征差异性研究

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水利水电技术（中英文） 2024年第1期55卷 157-166页

作者：王昌奎陈正峰巨广宏袁星宇钱瑞符文熹四川大学水利水电学院四川成都610065 四川大学水力学与山区河流开发保护国家重点实验室四川成都610065 中国建筑西南勘察设计研究院有限公司四川成都610081 中国电建集团西北勘测设计研究院有限公司陕西西安710065

【目的】水因素在滑坡灾前变形、运动解体、堆积过程中均发挥重要作用。从水力学角度,讨论河流流水对滑坡堆积、堵塞河道、形成堰塞坝的影响有重要减灾意义。【方法】基于2018年金沙江白格滑坡基础信息,聚焦有、无河流作用下滑坡运动演... 详细信息

【目的】水因素在滑坡灾前变形、运动解体、堆积过程中均发挥重要作用。从水力学角度,讨论河流流水对滑坡堆积、堵塞河道、形成堰塞坝的影响有重要减灾意义。【方法】基于2018年金沙江白格滑坡基础信息,聚焦有、无河流作用下滑坡运动演化过程中滑速、能量、形态特征差异,分别考虑白格滑坡处于无河流流水、有河流流水作用两种情况,开展了其运动过程的DEM模拟、CFD-DEM耦合模拟。【结果】根据计算捕获的速度、能量、形态等滑坡运动特征,量化揭示了河流流水对堵江滑坡的具体影响。【结论】研究发现:(1)河流流水影响下,滑坡体平均速度偏小,因河流影响的滑体部分较少,河流流速变化对滑坡体运动的影响较小且无明显规律;(2)动能和应变能在滑坡高速运动阶段受河流影响更明显,重力势能及能量损失在滑坡堆积阶段受河流影响更为明显,较无河流作用对白格滑坡应变能的影响最大,为1.80%。(3)河流对滑坡体形态特征的影响主要体现为最终纵向堆积长度的变化,滑坡计算启动后70 s,不受河流流水影响时,滑体纵向长度稳定在1.7 km,考虑河流流水影响下稳定在1.5 km,考虑河流影响下的模拟结果与实际情况较为吻合。

关键词：地质灾害白格滑坡河流作用流固耦合运动特征变形数值模拟

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反串式平面空心线圈电流传感器设计与性能分析

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电工技术 2024年第1期 136-141页

作者：张旭东袁兴宇李响云南电网有限责任公司电力科学研究院云南昆明650032

电网工作人员常带电设施作业,导致触电事故的现象时有发生,亟须合适的电场测量及安全预警装置。根据此类需求设计了一款基于空心线圈的新型传感器,为实际复杂工况下的电场测量提供新的解决方案。首先研究输电导线上的电流与其附近磁场... 详细信息

电网工作人员常带电设施作业,导致触电事故的现象时有发生,亟须合适的电场测量及安全预警装置。根据此类需求设计了一款基于空心线圈的新型传感器,为实际复杂工况下的电场测量提供新的解决方案。首先研究输电导线上的电流与其附近磁场强度的规律以及反向串联结构测量原理,结合合适的积分电路得到传感器在微分工作状态下的最优设计。然后根据电场测量反演规律,设计空心线圈型高频带电流传感器。最后通过分析空心线圈传感器的稳态和动态响应性能,验证了所设计的空心线圈传感器具有较宽的频带和测量范围,能有效抵御外界磁场干扰,且暂态与稳态响应性能满足实际需求。

关键词：空心线圈非接触测量频宽电磁感应

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Crowdsourcing RTK:a new GNSS positioning framework for building spatial high-resolution atmospheric maps based on massive vehicle GNSS data

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Satellite Navigation 2024年第1期5卷 91-108页

作者： Hongjin Xu xingyu Chen Jikun Ou Yunbin yuan State Key Laboratory of Geodesy and Earth’s Dynamics Innovation Academy for Precision Measurement Science and Technology(APM)Chinese Academy of Sciences(CAS)Wuhan430071China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijingChina

High-quality spatial atmospheric delay correction information is essential for achieving fast integer ambiguity resolution(AR)in precise positioning.However,traditional real-time precise positioning frameworks(i.e.,NRTK and PPP-RTK)depend on spatial low-resolution atmospheric delay correction through the expensive and sparsely distributed CORS network.This results in limited public appeal.With the mass production of autonomous driving vehicles,more cost-effective and widespread data sources can be explored to create spatial high-resolution atmospheric maps.In this study,we propose a new GNSS positioning framework that relies on dual base stations,massive vehicle GNSS data,and crowdsourced atmospheric delay correction maps(CAM).The map is easily produced and updated by vehicles equipped with GNSS receivers in a crowd-sourced way.Specifically,the map consists of between-station single-differenced ionospheric and tropospheric delays.We introduce the whole framework of CAM initialization for individual vehicles,on-cloud CAM maintenance,and CAM-augmented user-end positioning.The map data are collected and preprocessed in vehicles.Then,the crowdsourced data are uploaded to a cloud server.The massive data from multiple vehicles are merged in the cloud to update the CAM in time.Finally,the CAM will augment the user positioning performance.This framework forms a beneficial cycle where the CAM’s spatial resolution and the user positioning performance mutually improve each other.We validate the performance of the proposed framework in real-world experiments and the applied potency at different spatial scales.We highlight that this framework is a reliable and practical positioning solution that meets the requirements of ubiquitous high-precision positioning.

关键词： New GNSS positioning framework Spatial high-resolution atmospheric delay correction Crowdsourced atmospheric delay correction maps Crowdsourced ionosphere Crowdsourced troposphere Ubiquitous

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《症因脉治》从火与痰辨治咳嗽钩玄

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江苏中医药 2024年第5期56卷 65-67页

作者：袁星与张楚西姜廷桢熊桅朱振刚天津中医药大学第一附属医院天津300193 国家中医针灸临床医学研究中心天津300193

明代医家秦景明在其《症因脉治》一书中对咳嗽诊疗做了深刻的阐述,见解独到。秦氏将咳嗽先按病因分为外感和内伤,次辨虚实,并从火与痰的角度辨析咳嗽的病机。在治法上,秦氏主张以降火和治痰为主,认为咳嗽实火可泻、虚火可补,并注重从脾... 详细信息

明代医家秦景明在其《症因脉治》一书中对咳嗽诊疗做了深刻的阐述,见解独到。秦氏将咳嗽先按病因分为外感和内伤,次辨虚实,并从火与痰的角度辨析咳嗽的病机。在治法上,秦氏主张以降火和治痰为主,认为咳嗽实火可泻、虚火可补,并注重从脾论治。同时,秦氏亦从病之经络与脉症入手治疗咳嗽,值得我们细细揣摩,并运用于临床。

关键词：《症因脉治》秦景明咳嗽火痰

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