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Aminosaccharide-gold nanoparticle assemblies as narrow-spectrum antibiotics against methicillin-resistant Staphylococcus aureus

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Nano Research 2018年第12期11卷 6237-6243页

作者： Xinglong Yang Lingmin Zhang Xingyu Jiang CAS Center for Excellence in Nanoscience CAS Key Lab for Biological Effects of Nanomatefials and NanosafetyBeijing Engineering Research Center for BioNanotechnologyNational Center for NanoScience and TechnologyBeijing 100190China School of Chemistry and Chemical Engineering University of JinanJinan 250022China University of Chinese Academy of Sciences Beijing 100049China

Bacterial infection has continued to be a leading cause of death or disability worldwide because of antibiotic resistance. Antibiotic agents specific to certain taxa of bacteria, i.e., narrow-spectrum antibiotics, have become useful because they can kill bacteria without resulting in broad-spectrum drug resistance. In this study, we describe a series of antibiotics based on combining gold nanoparticles (AuNPs) with aminosaccharides, even though these AuNPs or aminosaccharides by themselves are ineffective against any bacteria. The AuNP-based multivalent aminosaccharides can effectively and selectively inhibit the growth of Gram-positive bacteria (including drug-resistant superbacteria). In particular, aminosaccharide-modified AuNPs are effective against methicillin-resistant Staphylococcus aureus (MRSA), a particularly hard-to-treat strain. This report carves out a way to explore antibiotics by combining AuNPs and an aminosaccharide as multivalent nanostructures, neither of which by itself is effective as an antibiotic.

关键词： gold nanoparticles multivalent aminosaccharides antibacterial activity methicillin-resistant Staphylococcus aureus (MRSA) antibiotics

Enhancing gene editing efficiency for cells by CRISPR/Cas9 system-loaded multilayered nanoparticles assembled via microfluidics

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Chinese Journal of Chemical Engineering 2021年第10期34卷 216-220页

作者： Xuanyu Li Qiang Feng Ziwei Han Xingyu Jiang Shenzhen Bay Laboratory Department of Biomedical EngineeringSouthern University of Science and TechnologyShenzhen 518055China

Most non-viral carriers for in vitro delivery of nucleic acids suffer from low efficiency of introducing m RNA and other nucleic acids,especially large m RNA.Cas9 protein is the nuclease part of the powerful gene-editing tool,CRISPR/Cas9 system,Cas9 m RNA is particularly large,thus presents a big challenge for delivery.We assembled a multilayered biodegradable nanocarrier to load Cas9 m RNA inside to protect Cas9 m RNA from degradation.We used a microfluidic chip to synthesize a small,positively charged,and degradable core to attract negatively charged Cas9 m RNA.The microfluidic assembly allows the core to be small enough to incorporate into a cationic liposome.The multilayered nanocarriers elevated the delivery efficiency of Cas9 m RNA by over 2 folds and increased the expression by over 5 folds compared to commercially used non-viral carriers.In addition,the multilayered nanocarriers do not require reduced serum medium for transfection.When using the standard complete medium for transfection,the multilayered nanocarriers could increase the expression of Cas9 m RNA by over 15 folds compared to commercially used non-viral carriers.The co-delivery of Cas9 m RNA and sg RNA via LRC elevated the gene-editing efficiency by 3 folds compared to that via commercially used non-viral carriers.Based on the higher transfection efficiency of Cas9 m RNA/sg RNA than commercially used non-viral carriers,these multilayered nanocarriers may have a good prospect as efficient commercial delivery carriers for Cas9 m RNA/sg RNA and other nucleic acids.

关键词： Nanotechnology Biomedical engineering Microfluidics CRISPR/Cas9 system Poly(lactic-co-glycolic acid)

Crowd sensing data delivery based on tangle DAG network

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The Journal of China Universities of Posts and Telecommunications 2023年第3期30卷 88-98页

作者： Chen Hui jiang Xiaoling Wu Tianting Mou xingyu Operation Monitoring Department Jilin Province Meteorological Information Network CenterChangchun 130062China Appraisal Department Jilin Province Meteorological Disaster Prevention Technology CenterChangchun 130062China

In view of the fact that current data delivery methods are not enough to meet the security requirements of today’s distributed crowd sensing,and the data delivery methods are not flexible enough,this paper proposes a crowd sensing data interaction method based on tangle directed acyclic graph(DAG)network.In this method,users and platforms are regarded as nodes of the network in the process of performing crowd sensing tasks.First,the heaviest chain is generated through the main chain strategy to ensure the stability of the network.Then,the hidden Markov model(HMM)prediction model is used to improve the correlation of the perceived data to improve the performance.Then,the confidential transaction and commitment algorithm is used to ensure the reliability of the transaction,overcome the security risks faced by the trusted third party,and simplify the group intelligence aware transaction mode.Finally,through simulation experiments,the security and feasibility of the group intelligence aware data delivery method based on tangle DAG network are verified.

关键词： data delivery crowd sensing tangle directed acyclic graph(DAG) confidential transactions

Point-of-Care Immunoassays with Tunable Detection Range for Detecting Infection in Intensive Care Unit

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CCS Chemistry 2021年第5期3卷 1562-1572页

作者： Lei Mou Zulan Li Jie Qi Xingyu Jiang Department of Biomedical Engineering Southern University of Science and TechnologyGuangdong 518055 National Center for NanoScience and Technology University of Chinese Academy of SciencesBeijing 100190 Reproduction Center of the 306th Hospital of People’s Liberation Army Beijing 100101

One of the difficulties of a multiplexed analytical assay is matching the concentration range of different markers.Here,the authors develop an automatic,multiplexed point-of-care(POC)immunoassay that allows detecting C-reactive protein(CRP),procalcitonin(PCT),and interleukin 6(IL-6)in 50μL serum samples with limits of detection 1.87μg/mL,0.17 ng/mL,and 49.75 pg/mL,respectively.The authors use electrospun fibers and surface chemistry of gold nanoparticles(AuNPs)to adjust the detection range of different biomarkers.The authors have proposed some new diagnostic indicators(mScore and mScorePlus)that combine the results of CRP,PCT,IL-6,and complete blood counts(CBCs).The authors also tracked changes in CRP,PCT,and IL-6 of patients in an intensive care unit(ICU).The authors find that mScore and mScorePlus have advantages in improving the diagnostic accuracy and providing more analytical information.mScore and mScorePlus are effective tools to detect infections,differentiate bacterial and viral infections,and monitor disease.Integrating multiple markers into one straightforward parameter is an effective method for analytical applications.The authors believe that this method provides a general way for chemists to develop increasingly accurate detection methods and indicators.

关键词： microfluidics immunoassay multiplexed assay intensive care unit point-of-care testing

High-performance organic electrochemical transistors gated with 3D-printed graphene oxide electrodes

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Nano Research 2023年第11期16卷 12689-12696页

作者： Xingyu Jiang Zhiqiang Liang Miao Wu Jie Lu Cheng Shi Qi Wang Zi Wang Zhen Jin Lin jiang Lizhen Huang Lifeng Chi Institute of Functional Nano&Soft Materials(FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials&DevicesSoochow UniversitySuzhou 215123China Suzhou Laboratory Suzhou 215123China Analysis and Test Center Soochow UniversitySuzhou 215123China

Organic electrochemical transistors(OECTs)have garnered significant interest due to their ability to facilitate both ionic and electronic transport.A large proportion of research efforts thus far have focused on investigating high-performance materials that can serve as mixed ion doping and charge transport layers.However,relatively less attention has been given to the gateelectrode materials,which play a critical role in controlling operational voltage,redox processes,and stability,especially in the context of semiconductor-based OECTs working in accumulation mode.Moreover,the demand for planarity and flexibility in modern bioelectronic devices presents significant challenges for the commonly used Ag/AgCl electrodes in OECTs.Herein,we report the construction of high-performance accumulation-mode OECTs by utilizing a gate electrode made of three-dimensional(3D)-printed graphene oxide.The 3D-printed graphene oxide electrode incorporating one-dimensional(1D)carbon nanotubes,is directly printed using an aqueous-based ink and showcases exceptional mechanical flexibility and porosity properties,enabling high-throughput preparation for both top gates and integrated planar architecture,as well as fast ion/charge transport.OECTs with high performance comparable to that of Ag/AgCl-gated OECTs are thus achieved and present promising feasibility for electrocardiograph(ECG)signal recording.This provides a promising choice for the application of flexible bioelectronics in medical care and neurological recording.

关键词： organic electrochemical transistors three-dimensional(3D)-printed graphene top electrodes electrocardiogram

An immunoassay based on lab-on-a-chip for simultaneous and sensitive detection of clenbuterol and ractopamine

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Chinese Chemical Letters 2020年第7期31卷 1835-1838页

作者： Qi Wang Jinqi Deng Yiping Chen Yunjing Luo Xingyu Jiang College of Life Science and Bioengineering Beijing University of TechnologyBeijing 100124China Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in NanoscienceNational Center for NanoScience and TechnologyBeijing 100190China Department of Biomedical Engineering Southern University of Science and Technology.Shenzhen 518055China University of Chinese Academy of Sciences Beijing 100049China College of Food Science and Technology.Huazhong Agricultural University Wuhan 430070China

Both clenbuterol(CLB)and ractopamine(RAC)areβ-adrenergic agonists.After long-term excessive intake,there will be adverse reactions such as headache,chest tightness,limb numbness,and serious lifethreatening.Simultaneous detection of CLB and RAC in related samples is of great importance for human health.In this work,we outline a microfluidics-based indirect competitive immunoassay(MICI)system that can sensitively detect residual CLB and RAC in pork,swine blood and swine urine.The rapid detection of multiple samples can be achieved in one chip,which greatly improves the detection efficiency.This method has good stability and reproducibility and the microfluidic chips are easy to manufacture.The linear ranges for CLB and RAC detection by MICI are 0.1-2.5 ng/mL and 0.1-5 ng/mL,and the limits of detection(LODs)are 0.094 ng/mL and 0.091 ng/mL,respectively.This straightforward and portable immunoassay system provides a good platform for rapid detection of harmful substances in food samples.

关键词： Microfluidic chips Simultaneous detection Indirect competitive immunoassay Clenbuterol Ractopamine

Human-induced changes in recent sedimentation rates in Bohai Bay, China： Implications for coastal development

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Science China Earth Sciences 2018年第10期61卷 1510-1522页

作者： Fu WANG Lizhu TIAN Xingyu Jiang William MARSHALL Hong WANG Tianjin Center China Geological Survey (CGS) Tianjin 300170 China Key Laboratory of Muddy Coast Geo-Environment China Geological Survey Tianjin 300170 China Plymouth University Plymouth Devon PL4 8AA United Kingdom

In many countries, coastal planners strive to balance the demands between civil, commercial strategy and environmental conversation interests for future development, particularly given the sea level rise in the 21 st century. Achieving a sustainable balance is often a dilemma, especially in low-lying coastal areas where dams in inland river basin are trapping significant amounts of fluvial sediments. We recently investigated the shore of Bohai Bay in northern China where there has been a severe increase in sea level following a program of large-scale coastal reclamation and infrastructure development over the last five decades. To investigate this trend, we obtained sediment cores from near-shore in Bohai Bay, which were dated by ^(137)Cs and ^(210)Pb radionuclides to determine the sedimentation rates for the last 50 years. The average sedimentation rates of Bohai Bay exceeded 10 mm yr^(-1) before 1963, which was much higher than the rate of local sea-level rise. However, our results showed an overall decreasing sedimentation rate after 1963, which was not able to compensate for the increasing relative sea-level rise in that period. In addition, our results revealed that erosion occurred after the 1980 s in the shallow sea area of Bohai Bay. We suggest that this situation places the Bohai Bay coast at a greater risk of inundation and erosion within the next few decades than previously thought, especially in the large new reclamation area. This study may be a case study for many other shallow sea areas of the muddy coast if the sea level continues to rise rapidly and the sediment delivered by rivers continues to decrease.

关键词：沉积率海湾沿海开发中国相对海平面 137Cs 放射性核种

The construction of drug-resistant cancer cell lines by CRISPR/Cas9 system for drug screening

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Science Bulletin 2018年第21期63卷 1411-1419页

作者： Lingmin Zhang Ying Li Qinghua Chen Yong Xia Wenfu Zheng Xingyu Jiang CAS Center for Excellence in Nanoscience Beijing Engineering Research Center for BioNanotechnologyCAS Key Laboratory for Biological Effects of Nanomaterials and NanosafetyNational Center for NanoScience and TechnologyBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Guangdong Provincial Key Laboratory of Molecular Target &Clinical Pharmacology School of Pharmaceutical Sciences and the Third &Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436China

Cancer therapy is often hampered by the rapid emergence of drug resistance. Drug-resistant cellular models are essential for understanding the drug resistance and developing new therapeutics. The low efficiency and long time required in creating these models are major obstacles hindering drug resistance research and drug screening. Herein, we report an approach that can accelerate(shortening the time from years to 3 weeks) the establishment of cancer cell line-based, inheritable drug resistance models by specific knockout of MED12 gene using CRISPR/Cas9 system. The resultant MED12^(KO) A375(melanoma)cell line was resistant to inhibitors of B-Raf proto-oncogene, serine/threonine kinase(BRAF), whereas the resultant MED12^(KO) PC9(non-small cell lung cancer) cell line was resistant to inhibitors of epidermal growth factor receptor(EGFR). Evaluation of anti-cancer drugs and their combinations shows that certain combinations of BRAF inhibitors and TGF-β receptor(TGF-βR) inhibitors are active in suppressing the growth of MED12^(KO) A375 cells, and a few combinations of EGFR inhibitors and TGF-βR inhibitors were active in suppressing the growth of MED12^(KO) PC9 cells. The drug-resistant models will be useful in screening novel drugs and drug combinations for multi-drug-resistant cancer therapy.

关键词： CRISPR/Cas9 Drug resistance Cancer Cell line Gene editing

Unexpected giant negative area compressibility in palladium diselenide

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National Science Review 2023年第9期10卷 146-153页

作者： Xingxing jiang Shengzi Zhang Dequan jiang Yonggang Wang Maxim S.Molokeev Naizheng Wang Youquan Liu xingyu Zhang Zheshuai Lin New Functional Crystals Group Key Laboratory of Functional Crystals and Laser TechnologyTechnical Institute of Physics and ChemistryChinese Academy of Sciences 2. Center of Materials Science and Optoelectronics EngineeringUniversity of Chinese Academy of Sciences University of Chinese Academy of Sciences Center for High Pressure Science &Technology Advanced Research School of Materials Science and Engineering Peking University Laboratory of Crystal Physics Kirensky Institute of PhysicsSB RAS 7. Department of Physics Far Eastern State Transport University 8. International Research Center of Spectroscopy and Quantum ChemistrySiberian Federal University

Negative area compressibility(NAC) is a counterintuitive ’squeeze-expand’ behavior in solids that is very rare but attractive due to possible pressure-response applications and coupling with rich physicochemical properties.Herein,NAC behavior is reported in palladium diselenide with a large magnitude and wide pressure range.We discover that,apart from the rigid flattening of layers that has been generally recognized,the unexpected giant NAC effect in PdSe2largely comes from anomalous elongation of intralayer chemical bonds.Both structural variations are driven by intralayer-to-interlayer charge transfer with enhanced interlayer interactions under pressure.Our work updates the mechanical understanding of this anomaly and establishes a new guideline to explore novel compression-induced properties.

关键词： negative area compressibility Lifshitz mechanism charge transfer