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Platelet-rich plasma combined with injectable hyaluronic acid hydrogel for porcine cartilage regeneration: a 6-month follow-up

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Regenerative Biomaterials 2020年第1期7卷 77-90页

作者： Wenqiang Yan xingquan xu Qian xu Ziying Sun Qing Jiang Dongquan Shi State Key Laboratory of Pharmaceutical Biotechnology Department of Sports Medicine and Adult Reconstructive SurgeryNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical School321 Zhongshan RoadNanjing 210008JiangsuChina Laboratory for Bone and Joint Disease Model Animal Research Center(MARC)Nanjing UniversityNanjing 210093JiangsuChina

Based on our previous study,the utilization of an ultraviolet light photo-cross-linkable hyaluronic acid(HA)hydrogel integrated with a small molecule kartogenin-encapsulated nanoparticles obtained good reconstruction of osteochondral defects in a rabbit model,indicating the superiority of injectable hydrogel-based scaffolds in cartilage tissue ***-rich plasma(PRP),rich in various growth factors,proteins and cytokines,is considered to facilitate cartilage healing by stimulating cell proliferation and inducing chondrogenesis in cartilage defect *** aim of this study was to test the therapeutic feasibility of autologous PRP combined with injectable HA hydrogel on cartilage *** focal cartilage defects with different critical sizes in the medial femoral condyle of a porcine model were *** 6 months,the minipigs were sacrificed for assessment of macroscopic appearance,magnetic resonance imaging,micro-computed tomography,histology staining and *** HA hydrogel combined with PRP-treated group showed more hyaline-like cartilage exhibited by macroscopic appearance and histological staining in terms of extracellular matrix and type II collagen without formation of hypertrophic cartilage,indicating its capacity to improve cartilage healing in the minipig model evaluated at 6 months,with full-thickness cartilage defect of 8.5mm diameter and osteochondral defect of 6.5mm diameter,5mm depth exhibiting apparent regeneration.

关键词： platelet-rich plasma hyaluronic acid hydrogel cartilage repair regenerative medicine

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Bone microenvironment regulative hydrogels with ROS scavenging and prolonged oxygen-generating for enhancing bone repair

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Bioactive Materials 2023年第6期24卷 477-496页

作者： Han Sun Juan xu Yangyufan Wang Siyu Shen xingquan xu Lei Zhang Qing Jiang State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive SurgeryDepartment of Orthopedic SurgeryNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical School321 Zhongshan RoadNanjing210008JiangsuPR China Branch of National Clinical Research Center for Orthopedics Sports Medicine and RehabilitationPR China Co-innovation Center of Neuroregeneration Nantong University9 Seyuan RoadNantong226019JiangsuPR China Articular Orthopaedics The Third Affiliated Hospital of Soochow University185 Juqian RoadChangzhou213003JiangsuPR China

Large bone defects resulting from fractures and disease are a major clinical challenge,being often unable to heal spontaneously by the body’s repair *** of evidence have shown that hypoxia-induced overproduction of ROS in bone defect region has a major impact on delaying bone ***,replenishing excess oxygen in a short time cause high oxygen tension that affect the activity of osteoblast precursor ***,reasonably restoring the hypoxic condition of bone microenvironment is essential for facilitating bone ***,we designed ROS scavenging and responsive prolonged oxygen-generating hydrogels(CPP-L/GelMA)as a“bone microenvironment regulative hydrogel”to reverse the hypoxic microenvironment in bone defects ***-L/GelMA hydrogels comprises an antioxidant enzyme catalase(CAT)and ROS-responsive oxygen-releasing nanoparticles(PFC@PLGA/PPS)co-loaded liposome(CCP-L)and GelMA *** hypoxic condition,CPP-L/GelMA can release CAT for degrading hydrogen peroxide to generate oxygen and be triggered by superfluous ROS to continuously release the oxygen for more than 2 *** prolonged oxygen enriched microenvironment generated by CPP-L/GelMA hydrogel significantly enhanced angiogenesis and osteogenesis while inhibited ***,CPP-L/GelMA showed excellent bone regeneration effect in a mice skull defect model through the Nrf2-BMAL1-autophagy ***,CPP-L/GelMA,as a bone microenvironment regulative hydrogel for bone tissue respiration,can effectively scavenge ROS and provide prolonged oxygen supply according to the demand in bone defect region,possessing of great clinical therapeutic potential.

关键词： Bone defect Hypoxic microenvironment Reactive oxygen species responsiveness Prolonged oxygen generation Brain and muscle arnt-like protein 1

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Advances of injectable hydrogel-based scaffolds for cartilage regeneration

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Regenerative Biomaterials 2019年第3期6卷 129-140页

作者： Jiawei Li Guojun Chen xingquan xu Peter Abdou Qing Jiang Dongquan Shi Zhen Gu Department of Sports Medicine and Adult Reconstructive Surgery Drum Tower HospitalSchool of MedicineNanjing University321 Zhongshan RoadNanjing 210008JiangsuP.R.China Department of Bioengineering University of CaliforniaLos Angeles410 Westwood PlazaLos AngelesCA 90095USA California NanoSystems Institute University of CaliforniaLos Angeles570 Westwood PlazaLos AngelesCA 90095USA Jonsson Comprehensive Cancer Center University of CaliforniaLos Angeles8-684 Factor BuildingLos AngelesCA 90095USA Center for Minimally Invasive Therapeutics University of CaliforniaLos Angeles570 Westwood PlazaLos AngelesCA 90095USA

Articular cartilage is an important load-bearing tissue distributed on the surface of diarthrodial *** to its avascular,aneural and non-lymphatic features,cartilage has limited self-regenerative *** date,the utilization of biomaterials to aid in cartilage regeneration,especially through the use of injectable scaffolds,has attracted considerable *** materials,therapeutics and fabrication approaches have emerged with a focus on manipulating the cartilage microenvironment to induce the formation of cartilaginous structures that have similar properties to the native *** particular,the design and fabrication of injectable hydrogel-based scaffolds have advanced in recent years with the aim of enhancing its therapeutic efficacy and improving its ease of *** review summarizes recent progress in these efforts,including the structural improvement of scaffolds,network cross-linking techniques and strategies for controlled release,which present new opportunities for the development of injectable scaffolds for cartilage regeneration.

关键词： drug delivery tissue engineering injectable hydrogel cartilage regeneration

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Cerium oxide nanozymes alleviate oxidative stress in tenocytes for Achilles tendinopathy healing

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Nano Research 2023年第5期16卷 7364-7372页

作者： xingquan xu Rongliang Wang Yixuan Li Rui Wu Wenjin Yan Sheng Zhao Quanyi Liu Yan Du Wenli Gong Weitong Li Hui Wei Dongquan Shi State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive SurgeryDepartment of Orthopedic SurgeryNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China Department of Biomedical Engineering College of Engineering and Applied SciencesNanjing National Laboratory of MicrostructuresJiangsu Key Laboratory of Artificial Functional MaterialsNanjing UniversityNanjing 210023China State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China University of Science and Technology of China Hefei 230026China State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringChemistry and Biomedicine Innovation Center(ChemBIC)Nanjing UniversityNanjing 210023China

Background:Reactive oxygen species(ROS)is considered as ubiquitous and highly active chemicals that influence tendon integrity and orchestrate tendon *** significant recent advances in nanomaterials,cerium oxide nanoparticles(CeO_(2)NPs)exhibit superoxide dismutase-and catalase-like ***,we introduced a therapeutic approach of CeO_(2)NPs for Achilles tendinopathy(AT)***:CeO_(2)NPs were synthesized to examine their effect as ROS scavengers on AT healing in vitro and in *** mRNA levels of inflammatory factors were evaluated in AT after CeO_(2)NPs treatment in *** mechanisms underlying CeO_(2)NPs-mediated stimulation of NRF2 translocation and ERK signaling were verified through immunofluorescence and Western blot *** efficacy of CeO_(2)NPs was tested in an AT rat model in comparison with the ***:CeO_(2)NPs not only significantly scavenged multiple ROS and suppressed ROS-induced inflammatory reactions but also protected cell proliferation under oxidative stress induced by tert-butyl hydroperoxide(TBHP).Moreover,CeO_(2)NPs could promote NRF_(2)nuclear translocation for anti-oxidation and anti-inflammation through the ERK signaling *** a rat model of collagenase-induced tendon injuries,CeO_(2)NPs showed significant therapeutic efficacy by ameliorating tendon ***:The present study provides valuable insights into the molecular mechanism of CeO_(2)NPs to ameliorate ROS in tenocytes via the ERK/NRF_(2)signaling pathway,which underscores the potential of CeO_(2)NPs for application in the treatment of enthesopathy healing.

关键词： CeO_(2)nanozymes enthesiopathy NRF2 translocation ERK signaling

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Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma

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Engineered Regeneration 2024年第2期5卷 199-209页

作者： Feng Liang xueying An Ruoxi Wang Wenshu Wu Lin Yang Yixin Zheng Qing Jiang xingquan xu Danni Zhong Min Zhou Eye Center The Second Affiliated HospitalZhejiang University School of MedicineHangzhou310000PR China Department of Orthopedics The First Affiliated HospitalZhejiang University School of MedicineHangzhou310000PR China State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive SurgeryDepartment of Orthopedic SurgeryNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing210008PR China Zhejiang University-University of Edinburgh Institute(ZJU-UoE Institute) Zhejiang University School of MedicineZhejiang UniversityHaining314400PR China State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive SurgeryDepartment of Orthopedic SurgeryNanjing Drum Tower HospitalClinical College of Nanjing Medical UniversityNanjing210008PR China Institute of Translational Medicine Zhejiang UniversityHangzhou310029PR China Zhejiang University-Ordos City Etuoke Banner Joint Research Center Zhejiang UniversityHaining314400PR China

Osteosarcoma(OS)is one of the most common malignant tumors in children and young *** chemotherapy and other therapies are limited by low therapeutic efficiency,severe side effects and single therapeutic function,it is of high value to develop innovative therapies for precise and efficient treatment of ***,natural photo-synthetic microalgae(***,CV)were utilized as carriers for the chemotherapeutic agent doxorubicin(DOX)to create a multifunctional therapeutic platform(CV@DOX)for the photo-modulation of the tumor microenvi-ronment(TME)and synergistic chemo-photodynamic therapy of ***@DOX exhibited rapid drug release behavior in the acidic TME,improving the efficiency of chemotherapy against tumors and reducing side effects on other normal *** 650 nm laser irradiation,CV@DOX demonstrated the ability to effectively generate oxygen to alleviate tumor hypoxia and utilize the photosensitizing properties of chlorophyll in CV to produce an increased amount of reactive oxygen species(ROS),thereby enhancing photodynamic therapy(PDT).CV@DOX-mediated synergistic chemo-photodynamic therapy demonstrated efficacy in halting tumor progression in an orthotopic osteosarcoma mouse model by promoting tumor cell apoptosis,inhibiting tumor proliferation and ***,chlorophyll-assisted fluorescence imaging enabled monitoring of the distribution of CV@DOX in osteosarcoma after ***,CV@DOX did not cause significant hematological and tissue toxicity,and prevented DOX-induced cardiotoxicity,showing good in vivo ***,this work presents a novel TME-responsive and TME-modulating platform for imaging-guided multimodal osteosar-coma treatment.

关键词： Osteosarcoma Tumor hypoxia Photodynamic Chemotherapy Synergistic therapy

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Targeting macrophagic SHP2 for ameliorating osteoarthritis via TLR signaling

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Acta Pharmaceutica Sinica B 2022年第7期12卷 3073-3084页

作者： Ziying Sun Qianqian Liu Zhongyang Lv Jiawei Li xingquan xu Heng Sun Maochun Wang Kuoyang Sun Tianshu Shi Zizheng Liu Guihua Tan Wenqiang Yan Rui Wu Yannick Xiaofan Yang Shiro Ikegawa Qing Jiang Yang Sun Dongquan Shi State Key Laboratory of Pharmaceutical Biotechnology Department of Sports Medicine and Adult Reconstructive SurgeryNanjing Drum Tower Hospitalthe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China Laboratory for Bone and Joint Disease Model Animal Research Center(MARC)Nanjing UniversityNanjing 210093China State Key Laboratory of Pharmaceutical Biotechnology Department of Biotechnology and Pharmaceutical SciencesSchool of Life ScienceNanjing UniversityNanjing 210023China Drum Tower of Clinical Medicine Nanjing Medical UniversityNanjing 210008China Laboratory for Bone and Joint Diseases RIKEN Center for Integrative Medical Science(IMSRIKEN)Tokyo 108-8639Japan

Osteoarthritis(OA),in which M1 macrophage polarization in the synovium exacerbates disease progression,is a major cause of cartilage degeneration and functional *** strategies of OA designed to interfere with the polarization of macrophages have rarely been ***,we report that SHP099,as an allosteric inhibitor of src-homology 2-containing protein tyrosine phosphatase 2(SHP2),attenuated osteoarthritis progression by inhibiting M1 macrophage *** demonstrated that M1 macrophage polarization was accompanied by the overexpression of SHP2 in the synovial tissues of OA patients and OA model *** to wild-type(WT)mice,myeloid lineage conditional Shp2 knockout(c KO)mice showed decreased M1 macrophage polarization and attenuated severity of synovitis,an elevated expression of cartilage phenotype protein collagen II(COL2),and a decreased expression of cartilage degradation markers collagen X(COL10)and matrix metalloproteinase3(MMP3)in OA *** mechanistic analysis showed that SHP099 inhibited lipopolysaccharide(LPS)-induced Toll-like receptor(TLR)signaling mediated by nuclear factor kappa B(NF-κB)and PI3K—AKT ***,intra-articular injection of SHP099 also significantly attenuated OA progression,including joint synovitis and cartilage *** results indicated that allosteric inhibition of SHP2 might be a promising therapeutic strategy for the treatment of OA.

关键词： SHP2 SHP099 Osteoarthritis Synovitis Toll-like receptor signaling Macrophage Cartilage degradation M1 macrophage polarization

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职业技术学院计算机技能多媒体教学研究

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中国教育技术装备 2013年第21期 124-125页

作者：许兴权泉州工艺美术职业学院

随着时代的进步与发展,现代教学技术理论的不断深化,多媒体辅助教学显得更加重要,已成为教学工作的坚实臂膀。针对职业技术学院计算机技能教学,对多媒体辅助教学的重要性进行总结和研究。

关键词：职业技术学院多媒体计算机教学

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图书管理系统设计

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科学之友 2007年第08B期 96-96,98页

作者：许兴权福建省德化县德化陶瓷职业技术学院福建德化362500

文章通过对数据库在图书管理中的作用,详细叙述了图书管理系统的操作方法。

关键词：图书管理数据库操作

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会计原则对信息质量的负面影响

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辽宁行政学院学报 2002年第4期4卷 60-61页

作者：徐兴权辽宁行政学院辽宁沈阳110161

会计信息的质量不高和会计信息失真是目前存在的一个较为普遍的问题 ,虽经多方治理 ,仍未得到根本改观。造成信息失真的原因是多方面的 ,但可归结为两类 :一类是由于弄虚作假造成的 ,即故意制造虚假的信息 ;另一类则是由于会计本身的不... 详细信息

会计信息的质量不高和会计信息失真是目前存在的一个较为普遍的问题 ,虽经多方治理 ,仍未得到根本改观。造成信息失真的原因是多方面的 ,但可归结为两类 :一类是由于弄虚作假造成的 ,即故意制造虚假的信息 ;另一类则是由于会计本身的不确定性和会计系统内在缺陷性等造成的。本文拟从会计核算原则 ,特别是确认、计量原则本身的缺陷来探讨信息失真的原因 ,进而提出相应的改进建议。

关键词：会计信息失真会计原则会计系统会计核算原则计量原则确认信息质量故意弄虚作假内在缺陷

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全膝关节置换术股骨髓外定位系统的研制及初步临床应用

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中华骨科杂志 2016年第15期36卷 955-963页

作者：徐志宏徐嘉诚陈东阳史冬泉戴进徐兴全蒋青南京大学医学院附属鼓楼医院运动医学及成人重建外科 210008

目的探讨应用自主研发的髓外定位系统进行全膝关节置换术股骨远端定位截骨的可行性及其初步临床应用疗效。方法2015年1至8月采用自主研发的髓外定位器械进行股骨远端截骨全膝关节置换的连续晚期骨关节炎或类风湿关节炎病例20例（髓外定... 详细信息

目的探讨应用自主研发的髓外定位系统进行全膝关节置换术股骨远端定位截骨的可行性及其初步临床应用疗效。方法2015年1至8月采用自主研发的髓外定位器械进行股骨远端截骨全膝关节置换的连续晚期骨关节炎或类风湿关节炎病例20例（髓外定位组），男3例，女17例；年龄52-82岁，平均70．1岁。2015年4至9月采用常规髓内定位进行股骨远端截骨全膝关节置换的晚期骨关节炎或类风湿关节炎病例20例（髓内定位组），男5例，女15例；年龄52-87岁，平均65．9岁。髓外定位组术前行双侧髋关节CT扫描明确股骨头中心与髂前上棘的关系，术中参考双侧髂前上棘定位股骨头中心、以内外上髁连线中点确定膝关节中心，髓外定位器械参考股骨头与膝关节中心连线放置并完成股骨远端截骨。比较两组术中出血量、术后引流量、术后1周内冠状面及矢状面假体力线，术后1周、6周和3个月的屈伸活动度。结果两组患者年龄、术前髋膝踝角（hip-knee—ankle angle，HKA）、美国特种外科医院（Hospital for Special Surgery，HSS）膝关节评分、体重指数、手术时间差异无统计学意义。髓外定位组与髓内定位组术中出血量分别为（121±64）ml和（177±47）ml（P〈0．05），术后引流量分别为（181±149）ml和（292±156）ml（P〈0．05）；术后股骨假体冠状面角分别为89．8°±2．1°和89．8°±2．2°（P〉0．05），股骨假体屈曲角分别为-0．8°±2．2°和2．5°±2．1°（P〈0．05）；术后1周屈伸活动度分别为103．8°±7．8°和102．5°±7．2°（P〉0．05）、术后6周分别为108．5°±8．0°和108．3°±7．4°、3个月分别为117．0°±7．1°和114．5°±8．1°（P〉0．05）。结论自主研发的髓外定位系统具有操作可行性，其矢状面定位精确度较髓内定位更高，术中出血量更小，术后早期膝关节活动度与采用髓内定位者相同。

关键词：关节成形术,置换,膝定位标记股骨

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