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Functional Analysis of the Promoter of Pollen-specific wheat PSG076 gene

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Functional Analysis of the Promoter of Pollen-specific wheat...

锁定加压钢板内固定治疗胫骨平台骨折临床分析

2012年湖北生物产业发展高端论坛暨湖北省生物工程学会2012年度学术交流会

作者： Ling Chen Yingjie Miao Cheng Wang Peipei Su Tianheng Li Rong Wang xinglong hao Guangxiao Yang Guangyuan He Chunbao Gao The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology Chinese National Center of Plant Gene Research(Wuhan) HUST PartCollege of Life Science and TechnologyHuazhong University of Science & Technology(HUST)

PSG076 is a wheat pollen-specific *** isolated the PSG076 promoter from wheat via the inverse PCR(IPCR) *** analysis showed that the promoter contained several cis-acting elements(AGAAA,GTGA,AAATGA and AGGTCA) which required for pollen-specific expression or involved in enhancing gene expression in *** ascertain the regulatory function of PSG076 promoter,the region upstream of the ATG start codon was fused with the P-glucuronidase(GUS) reporter gene and introduced into *** activity was only detected in pollen of transgenic *** GUS staining was found in other *** analysis in different stages of pollen development showed that GUS activity was firstly detected in late bicellular pollen grains and then increased rapidly in mature *** activity was also detected in pollen tubes of transgenic *** analysis of PSG076 promoter showed that the 0.4 kb fragment have stronger activity than 1.4 kb full-length and was enough to drive pollen-specific *** results confirm that PSG076 promoter is pollen-specific in late stages of promoter and pollen tube development.

关键词： Wheat Pollen-specific promoter PSG076 gene GUS Genetic transformation

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包头医学院学报 2010年第1期26卷 37-38,41页

作者：王宝成杨建国郝兴龙鄂尔多斯乌审旗人民医院外科内蒙古乌审旗017300 呼和浩特市第一医院

目的：探讨锁定加压钢板治疗胫骨平台骨折的方法和疗效。方法：运用锁定加压钢板治疗胫骨平台骨折46例，术后早期结合CPM机行膝关节功能锻炼。结果：45例患者获得8～24个月的随访（平均16个月），术后骨折愈合时间平均为5个月。膝关节... 详细信息

目的：探讨锁定加压钢板治疗胫骨平台骨折的方法和疗效。方法：运用锁定加压钢板治疗胫骨平台骨折46例，术后早期结合CPM机行膝关节功能锻炼。结果：45例患者获得8～24个月的随访（平均16个月），术后骨折愈合时间平均为5个月。膝关节功能参照Merchant功能评定标准，优27例、良15例、可3例，优良率91．3％。结论：锁定加压钢板治疗胫骨平台骨折疗效确切，具有对机体创伤小，固定牢靠，关节功能恢复快，并发症少等优点。

关键词：胫骨平台骨折锁定加压钢板内固定

A CADe system for nodule detection in thoracic CT images based on artificial neural network

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Science China(Information Sciences) 2017年第7期60卷 177-194页

作者： xinglong LIU Fei HOU Hong QIN Aimin hao State Key Laboratory of Virtual Reality Technology and Systems Beihang University School of Computer Science and Engineering Nanyang Technological University Department of Computer Science Stony Brook University

Lung cancer has been the leading cause of cancer-related deaths in 2015 in United States. Early detection of lung nodules will undoubtedly increase the five-year survival rate for lung cancer according to prior studies. In this paper, we propose a novel rating method based on geometrical and statistical features to extract initial nodule candidates and an artificial neural network approach to the detection of lung nodules. The novel method is solely based on 3D distribution of neighboring voxels instead of user-specified features. During initial candidates detection, we combine organized region properties calculated from connected component analysis with corresponding voxel value distributions from statistical analysis to reduce false positives while retaining true nodules. Then we devise multiple artificial neural networks(ANNs) trained from massive voxel neighbor sampling of different types of nodules and organize the outputs using a 3D scoring method to identify final nodules. The experiments on 107 CT cases with 252 nodules in LIDC-IDRI data sets have shown that our new method achieves sensitivity of 89.4% while reducing the false positives to 2.0 per case. Our comprehensive experiments have demonstrated our system would be of great assistance for diagnosis of lung nodules in clinical treatments.

关键词： artificial neutral network Lung nodule computed tomography computer aided detection nodule detection

Functional analysis of the promoter of a pollen- specific PSG076 gene in wheat(Triticum aestivum L.)

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Functional analysis of the promoter of a pollen- specific PS...

Effects of temperature and alloying content on the phase transformation and{10■1}twinning in Zr during rolling

第十三届全国植物基因组学大会

作者： Ling Chen,Yingjie Miao,Cheng Wang,Peipei Su,Tianheng Li,Rong Wang,xinglong hao, Guangxiao Yang~*,Guangyuan He~* The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology,Chinese National Center of Plant Gene Research(wuhan) Hust Part,College of Life Science and Technology,Huazhong University of Science & Technology,Wuhan 430074, China

PSG076 is a wheat pollen-specific gene,the promoter of which was isolated from wheat via the inverse PCR(IPCR) *** analysis showed that the promoter contained several cis-acting elements(AGAAA,GTGA,AAATGA and AGGTCA) ...

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Journal of Materials Science & Technology 2020年第6期41卷 76-80页

作者： xinglong An hao Zhang Song Ni Xiaoqin Ou Xiaozhou Liao Min Song State Key Laboratory of Powder Metallurgy Central South UniversityChangsha 410083China School of Aerospace Mechanical and Mechatronic EngineeringThe University of SydneySydneyNSW 2006Australia

The effects of temperature and Ti content on the deformation mechanisms of pure Zr and Zr-Ti alloys were investigated by transmission electron *** results indicate the existence of a relation between deformation-induced phase transformation from a hexagonal close-packed structure to a facecentered cubic structure and{10■1}deformation *** is,when one is suppressed,the other will be *** phase transformation was suppressed while the{10■1}compressive twinning was promoted with increasing the rolling temperature and/or Ti *** can be attributed to the activation of basaldislocations at high temperature and the increased stacking fault energy with Ti content.

关键词： Zr Zr-Ti alloy Twinning Phase transformation Stacking fault energy

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无比对样本的STR混合分型分析策略

中国法医学杂志 2017年第6期32卷 645-648页

作者：王禹毛坤云陈嘉佳郝兴龙贾润镇江市公安局刑警支队江苏镇江212000

目的充分利用数据库的比对功能,指导无比对样本混合分型的分析、数据库比对和比对结果的筛选。方法使用联合包含概率描述混合分型的识别力。利用联合被包含概率来估计被混合分型包含的样本的可靠性。结果混合分型的联合包含概率小于10^(... 详细信息

目的充分利用数据库的比对功能,指导无比对样本混合分型的分析、数据库比对和比对结果的筛选。方法使用联合包含概率描述混合分型的识别力。利用联合被包含概率来估计被混合分型包含的样本的可靠性。结果混合分型的联合包含概率小于10^(-7)时具备入库比对的价值。混合分型单个基因座上能确定的等位基因大于等于2个时,增加一个额外等位基因,不会过多降低识别力。数据库检索获得多个无容差比中结果后,可以根据联合被包含概率来进行排序,快速找到最有价值样本。

关键词：法医物证学 STR 混合分型数据库比对

Functional identification of MdSMXL8.2,the homologous gene of strigolactones pathway repressor protein gene in Malus×domestica

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Horticultural Plant Journal 2021年第4期7卷 275-285页

作者： Weijian Sun xinglong Ji Laiqing Song Xiaofei Wang Chunxiang You Yujin hao State Key Laboratory of Crop Biology Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and EfficiencyCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai’anShandong271018China Yantai Academy of Agricultural Sciences YantaiShandong265599China

A homologous gene of strigolactones repressor protein gene SMXL7/D53,MdSMXL8.2(GenBank accession No.:MD07G1222400),was cloned from‘Royal Gala’apple(Malus×domestica Borkh.)in this *** sequence analysis revealed that the length of this gene was 3243 bp,which encoded 1080 amino acids,and had a protein molecular mass of∼110 *** phylogenetic tree analysis indicated that the MdSMXL8.2 exhibited the highest sequence similarity with Arabidopsis *** protein conserved domain analysis revealed that the MdSMXL8.2 contained two ClpA *** prediction of the secondary and tertiary structures of the MdSMXL8.2 indicated that it contained 34.54%αhelix,3.43%β-sheet,and 11.76%extended *** in-silico analysis suggested that the promoter sequence of MdSMXL8.2 contained several typical cisacting elements,including abscisic acid(ABA),gibberellin(GA),ethylene,auxin,jasmonic acid(JA),salicylic acid(SA),drought,and heat stressresponsive *** real-time(qRT)-PCR analyses revealed that MdSMXL8.2 was expressed in different apple tissues,with the highest transcript level found in the *** expression of MdSMXL8.2 was significantly induced by exogenous ABA,PEG and mannitol,while exogenous NaCl significantly inhibited MdSMXL8.2 *** growing status of MdSMXL8.2-overexpressed Orin apple callus was worse than the wild type(WT)after NaCl treatment and had a higher malondialdehyde(MDA)content and relative conductance(REC).Additionally,MdSMXL8.2-overexpressed Arabidopsis exhibited shorter root length and a reduction in fresh weight under salt stress,indicating that MdSMXL8.2 negatively regulated salt tolerance in apples.

关键词： MdSMXL8.2 Apple Strigolactones Salt stress

Functional analysis of cis-regulatory elements within the promoter of the wheat pollen-specific gene PSG076

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Functional analysis of cis-regulatory elements within the pr...

Electrode particulate materials for advanced rechargeable batteries:A review

第十一届全国植物基因组学大会

作者： Ling Chen,Cheng Wang,Tianheng Li,Rong Wang,xinglong hao,Cheng Ma,Guangxiao Yang,Guangyuan He~* P.R.China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory,Chinese MoST International Cooperation Base of Science and Technology(Genetic Engineering),Chinese MoE Key Laboratory of Molecular Biophysics,College of Life Science and Technology,HuazhongUniversity of Science and Technology,Wuhan 430074,P.R.China

Pollen,as the male gametophyte,is very important during plant sexual *** of pollen-specific genes and their promoters would shed light on further understanding the regulatory mechanism of pollen development and sexual...

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Particuology 2024年第3期86卷 160-181页

作者： Mingyang Ma Miao Du Yan Liu Hongyan Lü Jialin Yang Zelin hao Jinzhi Guo xinglong Wu MOE Key Laboratory for UV Light-Emitting Materials and Technology Northeast Normal UniversityChangchunJilin130024China Department of Chemistry Northeast Normal UniversityChangchunJilin130024China

The demand for large-scale energy storage is increasing due to the decreasing non-renewable resources and deteriorating environmental *** rechargeable batteries with high energy density and long cycle performance is an ideal choice to meet the demand of energy storage *** development of excellent electrode particles is of great significance in the commercialization of nextgeneration *** ideal electrode particles should balance raw material reserves,electrochemical performance,price and environmental *** them,the development of electrode particulate materials with excellent electrochemical properties is the top priority at *** this review,the typical researches of electrode materials are summarized in terms of crystal structure,morphology,pore structure,surface and interface ***,the structural characteristics and improvement methods of transition metal oxides,polyanionic compounds,Prussian blue and their analogues are ***,the different effects of particulate morphology,pore,surface and interface structure on the performance of electrode materials are *** designing high-performance electrode materials,preparation route should be set according to the particle properties of the materials and the synergistic effect of various optimization methods should be *** the same time,in addition to the electrode materials,other components of the rechargeable batteries,such as current collector,separator and electrolytes,should be optimized to improve the overall performance of the *** review would provide important guiding principle for designing high-performance electrode particulate materials.

关键词： Particulate properties Electrode materials Rechargeable batteries