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SARS-CoV-2-specific immune response in COVID-19 convalescent individuals

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Signal Transduction and Targeted Therapy 2021年第8期6卷 2484-2493页

作者： Yunbao Pan Xianghu Jiang Liu Yang Liangjun Chen Xiaojiao Zeng Guohong Liu Yueting Tang Chungen Qian Xinming wang Fangming Cheng Jun Lin xinghuan wang Yirong Li Department of Laboratory Medicine Zhongnan Hospital of Wuhan UniversityWuhan UniversityWuhanHubeiChina Wuhan Research Center for Infectious Diseases and Cancer Chinese Academy of Medical SciencesWuhanChina Department of Radiology Zhongnan Hospital of Wuhan UniversityWuhan UniversityWuhanChina The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics&Molecular Imaging Key Laboratory Systems Biology ThemeDepartment of Biomedical EngineeringCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhanHubeiChina Reagent R&D Center Autobio Diagnostics Co.LtdZhengzhouHenanChina Reagent R&D Center Shenzhen YHLO Biotech Co.LtdShenzhenGuangdongChina Department of Gastroenterology Zhongnan Hospital of Wuhan UniversityWuhan UniversityWuhanChina Center for Evidence-Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhan UniversityWuhanChina Department of Urology Zhongnan Hospital of Wuhan UniversityWuhan UniversityWuhanChina

We collected blood from coronavirus disease 2019(COVID-19)convalescent individuals and investigated SARS-CoV-2-specific humoral and cellular immunity in these discharged ***-up analysis in a cohort of 171 patients at 4-11 months after the onset revealed high levels of IgG antibodies.A total of 78.1%(164/210)of the specimens tested positive for neutralizing antibody(NAb).SARS-CoV-2 antigen peptide pools-stimulated-IL-2 and-IFN-y response can distinguish COVID-19 convalescent individuals from healthy ***,NAb survival was significantly affected by the antigen peptide pools-stimulated-IL-2 response,-IL-8 response,and-IFN-y *** antigen peptide pools-activated CD8+T cell counts were correlated with *** antigen peptide pools-activated natural killer(NK)cell counts in convalescent individuals were correlated with NAb and disease *** data suggested that the development of NAb is associated with the activation of T cells and NK *** work provides a basis for further analysis of the protective immunity to SARS-CoV-2 and for understanding the pathogenesis of *** also has implications for the development of an effective vaccine for SARS-CoV-2 infection.

关键词： immunity protective vaccine

Personnel protection strategy for healthcare workers in Wuhan during the COVID-19 epidemic

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Precision Clinical Medicine 2020年第3期3卷 169-174页

作者： Fan Fan Hou Fuling Zhou Xin Xu Daowen wang Gang Xu Tao Jiang Sheng Nie XiaoyanWu Chanjun Ren Guangyu wang Johnson Yiu-Nam Lau xinghuan wang Kang Zhang Nanfang Hospital Southern Medical UniversityNational Clinical Research Center for Kidney DiseaseState Key Laboratory of Organ Failure ResearchGuangzhou 510515China Zhongnan Hospital of Wuhan University Wuhan 430000China Tongji Hospital Huazhong University of Science and TechnologyWuhan 430030China Jingzhou Central Hospital Jingzhou 434020China Wuhan Kingmed Medical Laboratory WuhanChina Department of Computer Science and Technology Tsinghua UniversityBeijing 100084China Department of Applied Biology and Chemical Technology Hong Kong Polytechnic UniversityHung HomHong KongChina Center for Biomedicine and Innovations Faculty of MedicineMacao University of Science and TechnologyMacaoChina

Objective:To identify the effectiveness of a personnel protection strategy in protection of healthcare workers from SARS-CoV-2 ***:During the COVID-19 pandemic,943 healthcare staff sent from Guangzhou to Wuhan to care for patients with suspected/confirmed COVID-19 received infection precaution training before their mission and were equipped with Level 2/3 personal protective equipment(PPE),in accordance with guidelines from the National Health Commission of *** conducted a serological survey on the cumulative attack rate of SARS-CoV-2 among the healthcare workers sent to Wuhan and compared the seropositive rate to that in local healthcare workers from Wuhan and ***:Serial tests for SARS-CoV-2 RNA and tests for SARS-CoV-2 immunoglobulin M and G after the 6-8 week mission revealed a zero cumulative attack *** the local healthcare workers inWuhan and Jingzhou of Hubei Province,2.5%(113 out of 4495)and 0.32%(10 out of 3091)had RT-PCR confirmed COVID-19,*** seropositivity for SARS-CoV-2 antibodies(IgG,IgM,or both IgG/IgM positive)was 3.4%(53 out of 1571)in local healthcare workers from Wuhan with Level 2/3 PPE working in isolation areas and 5.4%(126 out of 2336)in healthcare staff with Level 1 PPE working in non-isolation medical areas,*** and relevance:Our study confirmed that adequate training/PPE can protect medical personnel against SARS-CoV-2.

关键词： COVID-19 healthcare workers personal protective equipment(PPE)

Accurate and efficient pulmonary CT imaging workflow for COVID-19 patients by the combination of intelligent guided robot and automatic positioning technology

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Intelligent Medicine 2021年第1期1卷 3-9页

作者： Yadong Gang Xiongfeng Chen Hanlun wang Jianying Li Ying Guo Bin Wen Jinxiang Hu Haibo Xu xinghuan wang Department of Radiology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Radiology Puren Hospital affiliated to Wuhan University of Science and TechnologyWuhan 430080China GE Healthcare Computed Tomography Research CenterBeijing 100176China TAMI Intelligence Technology(Beijing)Co.Ltd Beijing 100176China Department of Urology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Center for Evidence-based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071China

Background:The ongoing coronavirus disease 2019(COVID-19)pandemic has put radiologists at a higher risk of infection during the computer tomography(CT)examination for the *** help settling these problems,we adopted a remote-enabled and automated contactless imaging workflow for CT examination by the combination of intelligent guided robot and automatic positioning technology to reduce the potential exposure of radiologists to 2019 novel coronavirus(2019-nCoV)infection and to increase the examination efficiency,patient scanning accuracy and better image quality in chest CT ***:From February 10 to April 12,2020,adult COVID-19 patients underwent chest CT examinations on a CT scanner using the same scan protocol except with the conventional imaging workflow(CW group)or an automatic contactless imaging workflow(AW group)in Wuhan Leishenshan Hospital(China)were retrospectively and prospectively enrolled in this *** total examination time in two groups was recorded and *** patient compliance of breath holding,positioning accuracy,image noise and signal-to-noise ratio(SNR)were assessed by three experienced radiologists and compared between the two ***:Compared with the CW group,the total positioning time of the AW group was reduced((118.0±20.0)s vs.(129.0±29.0)s,P=0.001),the proportion of scanning accuracy was higher(98%vs.93%),and the lung length had a significant difference((0.90±1.24)cm vs.(1.16±1.49)cm,P=0.009).For the lesions located in the pulmonary centrilobular and subpleural regions,the image noise in the AW group was significantly lower than that in the CW group(centrilobular region:(140.4±78.6)HU vs.(153.8±72.7)HU,P=0.028;subpleural region:(140.6±80.8)HU vs.(159.4±82.7)HU,P=0.010).For the lesions located in the peripheral,centrilobular and subpleural regions,SNR was significantly higher in the AW group than in the CW group(centrilobular region:6.6±4.3 vs.4.9±3.7,P=0.006;subpleural region:6.4±4.4 vs.4.8±4.0,P<0.001).Co

关键词： Coronavirus disease 2019 Artificial intelligence Robotics Computer tomography

临床路径的制订与管理系列研究之一:临床路径的制订理论、方法、流程与建议

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医学新知 2024年第2期34卷 179-190页

作者：易烨林珂雯娄佳奡彭淑桢靳英辉冯佳佳张潘林丽开王行环武汉大学中南医院循证与转化医学中心武汉430071 武汉大学医学部武汉430071 武汉大学中南医院护理部武汉430071 武汉大学医院管理研究所武汉430071

临床路径的制订和应用是一个动态改进和发展的过程,临床路径制订原理来源于多个学科,同时在临床路径的临床应用中不断丰富完善。临床路径制订的方法学流程和报告学标准在国际上尚未统一。本文通过系统检索和筛选临床路径制订文献,最终确... 详细信息

临床路径的制订和应用是一个动态改进和发展的过程,临床路径制订原理来源于多个学科,同时在临床路径的临床应用中不断丰富完善。临床路径制订的方法学流程和报告学标准在国际上尚未统一。本文通过系统检索和筛选临床路径制订文献,最终确定5篇完整报告临床路径制订全过程的方法学文献,对其制订流程进行思路梳理,并综合其余文献分析,提出临床路径制订流程建议并进行讨论,以期为医院管理者制订临床路径提供参考。

关键词：临床路径制订与管理制订理论方法流程

Artificial intelligence-based approaches for COVID-19 patient management

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Intelligent Medicine 2021年第1期1卷 10-15页

作者： Lan Lan Wenbo Sun Dan Xu Minhua Yu Feng Xiao Huijuan Hu Haibo Xu xinghuan wang Department of Radiology Zhongnan Hospital of Wuhan UniversityNo.169 Donghu RoadWuchang DistrictWuhanHunan 430071China Center for Evidence-Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityNo.169 Donghu RoadWuchang DistrictWuhanHunan 430071China Department of Urology Zhongnan Hospital of Wuhan UniversityWuhanHunan 430071China

During the highly infectious pandemic of coronavirus disease 2019(COVID-19),artificial intelligence(AI)has provided support in addressing challenges and accelerating achievements in controlling this public health *** has been applied in fields varying from outbreak forecasting to patient management and drug/vaccine *** this paper,we specifically review the current status of AI-based approaches for patient *** and challenges still exist,and further needs are highlighted.

关键词： Coronavirus disease 2019 Artificial intelligence

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微创泌尿外科的现状及展望

中华实验外科杂志 2021年第6期38卷 991-995页

作者：王行环郑州大学第一附属医院骨科 450000

得益于电外科技术、数字影像技术、激光技术、人工智能的应用以及医疗设备的不断改进,近20年来,微创手术在外科领域获得了长足的进步和推广,而泌尿外科是其中最活跃的领域之一。本文将就微创技术在泌尿外科的应用和其展望作一简要介绍。

关键词：微创外科泌尿外科

临床路径的制订与管理系列研究之二:临床路径的信息化管理

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医学新知 2024年第3期34卷 322-329页

作者：林珂雯娄佳奡彭淑桢易烨靳英辉冯佳佳张潘熊浩贺谦王行环林丽开武汉大学医学部武汉430071 武汉大学中南医院护理部武汉430071 武汉大学中南医院循证与转化医学中心武汉430071 武汉大学医院管理研究所武汉430071 武汉大学中南医院信息中心武汉430071

信息化是临床路径发展的必然趋势,基于医院信息系统可实现临床路径信息化管理,并构建临床路径决策诊疗知识库,进而支持临床路径自适应性动态调整,是临床路径信息化管理的核心。本文对临床路径信息化管理的建设基础及临床路径知识库的构... 详细信息

信息化是临床路径发展的必然趋势,基于医院信息系统可实现临床路径信息化管理,并构建临床路径决策诊疗知识库,进而支持临床路径自适应性动态调整,是临床路径信息化管理的核心。本文对临床路径信息化管理的建设基础及临床路径知识库的构建进行阐述,以期从信息化管理的角度为临床路径的制订和管理提供参考。

关键词：临床路径制订与管理信息化知识库

轮对和轨道结构底部柔性对桥梁结构噪声的影响

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铁道学报 2022年第12期44卷 125-133页

作者：王星欢何远鹏张皓迪圣小珍西南交通大学牵引动力国家重点实验室四川成都610036 中国铁道科学研究院集团有限公司机车车辆研究所北京100081 成都市新筑路桥机械股份有限公司四川成都611430 上海工程技术大学城市轨道交通学院上海201620

目前对于桥梁结构噪声的研究大多采用有限元-边界元法,而且在进行轮轨力计算时把轮对和轨道结构底部看成是刚性,没有研究轮对柔性和轨道底部由桥梁提供的弹性对桥梁结构噪声的影响。基于此,以WJ-8扣件板式无砟轨道为例,利用频域轮轨相... 详细信息

目前对于桥梁结构噪声的研究大多采用有限元-边界元法,而且在进行轮轨力计算时把轮对和轨道结构底部看成是刚性,没有研究轮对柔性和轨道底部由桥梁提供的弹性对桥梁结构噪声的影响。基于此,以WJ-8扣件板式无砟轨道为例,利用频域轮轨相互作用模型及有限元-边界元法建立桥梁振动声辐射模型,研究轮对柔性、轨道结构底部柔性、列车运行速度等对桥梁结构噪声的影响。研究结果表明:若将轮对和轨道结构底部均考虑为刚性时,则会高估桥梁结构噪声达3.2 dB;随着列车运行速度的增加,桥梁结构噪声也增加,其增大程度主要取决于轮轨粗糙度谱;翼板对各个桥旁测点声压级的贡献量均不可忽略。

关键词：轮对柔性轨道结构底部柔性轮轨相互作用桥梁结构噪声有限元-边界元法

微小RNA-129在前列腺癌中的表达及其对前列腺癌细胞株PC-3增殖的影响

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中华实验外科杂志 2014年第12期31卷 2670-2672页

作者：张平王行环武汉大学中南医院泌尿外科 430071

目的观察微小RNA（miRNA,miR）-129在前列腺癌中的表达及其对前列腺癌细胞株PC-3增殖的影响.方法采用实时定量聚合酶链反应（Real-time PCR）技术检测37例前列腺癌及其癌旁组织中miR-129的表达.用miR-129模拟物片段（has-miR-129 mimi... 详细信息

目的观察微小RNA（miRNA,miR）-129在前列腺癌中的表达及其对前列腺癌细胞株PC-3增殖的影响.方法采用实时定量聚合酶链反应（Real-time PCR）技术检测37例前列腺癌及其癌旁组织中miR-129的表达.用miR-129模拟物片段（has-miR-129 mimic）转染前列腺癌PC-3细胞,Real-time PCR法检测转染后48 h细胞中miR-129的表达,细胞计数试剂盒（CCK-8）分别检测后续培养0、24、48、72 h后PC-3细胞增殖的变化.结果前列腺癌组织中miR-129的表达（1.088 1±0.578 5）低于相应的癌旁组织（1.498 6±0.782 8,P＜0.05）.has-miR-129 mimic转染后PC-3细胞中miR-129的表达量是对照组的435倍（P＜0.01）,后续培养48 h后转染组细胞吸光度值（0.714 7±0.072 1）明显低于对照组（0.878 4±0.057 3,P＜0.05）.结论 miR-129在前列腺癌组织中低表达,过表达miR-129可以抑制前列腺癌细胞增殖.

关键词：前列腺癌微小RNA-129 增殖