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Journal of Integrative Plant Biology 2010年第3期52卷 273-288页

作者： xingchun wang Li Xue Jiaqiang Sun Jianru Zuo College of Life Sciences Shanxi Agricultural University Taigu 030801 China State Key Laboratory of Plant Genomics and National Plant Gene Research Center Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China

Carbohydrate metabolism is central to plant growth and development. However, little is known about its role in embryogenesis. Here, we report the characterization of multiple alleles of the BRANCHING ENZYME1 （BE1） gene （also known as EMB2729）. The weak allele of be1-3, characterized by positional cloning, carries a single-nucleotide substitution in an exon-intron junction and shows various developmental defects during post-germination growth. This mutation causes a reduced level of BE1 mRNA that, likely generated from cryptically spliced pre-mRNA, contains a Glu-to-Lys substitution at codon 366. In four null alleles, BE1 is disrupted by T-DNA insertions, causing embryo developmental arrests at the heart stage. Light microscopy reveals reduced cell divisions and abnormal cell differentiation, thereby leading to defects in setting up the shoot apical meristem, embryonic vascular tissues and cotyledons. Overexpression of BE1 results in a pleiotropic phenotype, indicating that the fine-tuned BE1 level is crucial for plant growth and development. BE1 encodes a putative glycoside hydrolase that is highly conserved in higher plants. A BE1-GFP fusion protein, which is fully functional in complementing be1 mutants, is localized in plastids. The be1-3 phenotype can be partially rescued by glucose, fructose or sucrose, implying the involvement of BE1 in carbohydrate metabolism in plastids.

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ASYMPTOTICS OF THE SOLUTIONS TO STOCHASTIC WAVE EQUATIONS DRIVEN BY A NON-GAUSSIAN LéVY PROCESS

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Acta Mathematica Scientia 2019年第3期39卷 731-746页

作者： Yiming JIANG Suxin wang xingchun wang School of Mathematical Sciences and LPMC Nankai UniversityTianjin 300071China College of Science Civil Aviation University of ChinaTianjin 300300China School of International Trade and Economics University of International Business and EconomicsBeijing 100029China

In this article, we consider the long time behavior of the solutions to stochastic wave equations driven by a non-Gaussian Lévy process. We shall prove that under some appropriate conditions, the exponential stabilit... 详细信息

关键词： Stochastic wave equations non-Gaussian Lévy processes exponential stability second moment stability

Deletion of the Initial 45 Residues of ARR18 Induces Cytokinin Response in Arabidopsis

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Journal of Genetics and Genomics 2012年第1期39卷 37-46页

作者： Yan Liang xingchun wang Sulei Hong Yansha Li Jianru Zuo State Key Laboratory of Plant Genomics and National Plant Gene Research Center(Beijing) Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Graduate School of Chinese Academy of Sciences Beijing 100049China

The plant hormone cytokinin plays important roles in various aspects of plant growth and development. Cytokinin signaling is mediated by a multistep phosphorelay similar to bacterial two-component system. Type-B ARRs lie at the end of the cytokinin signaling, typically mediating tile output response. However, it is still unclear how type-B ARRs are regulated in response to cytokinin. Typical type-B ARR contains an N-terminal receiver domain and a C-terminal effector domain. In this study, we performed a genome-wild comparative analysis by overexpressing full length and C-terminal effector domain of seven representative type-B ARRs. Our results indicated that overexpression of C-terminal effector domain causes short primary roots and short hypocotyls without the addition of cytokinin, sug- gesting that the inhibitory role of the receiver domain in the activity of the effector domain is a common mechanism in type-B ARRs. To investigate how the receiver domain inhibits the activity of the effector domain, we performed a deletion analysis. We found that deletion of the initial 45 residues of ARR18 （the 45 residues from N-terminus） causes pleiotropic growth defects by directly inducing cytokinin responsive genes. Together, our results suggest that the initial 45 residues are critical for the inhibitory role of the receiver domain to the effector domain in ARRI8.

关键词： Cytokinin Response regulator ARRI8 Receiver domain

Inversion of IP-Affected TEM Responses and Its Application in High Polarization Area

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Journal of Earth Science 2021年第1期32卷 42-50页

作者： Qingquan Zhi Xiaohong Deng Junjie Wu Xiu Li xingchun wang Yi Yang Jie Zhang School of Geology Engineering and Geometrics Chang'an UniversityXi'an 710000China Institute of Geophysical and Geochemical Exploration Chinese Academy of Geological SciencesLangfang 065000China Laboratory of Geophysical Electromagnetic Probing Technologies Ministry of Land and ResourcesLangfang 065000China

The transient electromagnetic(TEM)responses measured in areas with highly pola-rizable media commonly show induced polarization(IP)effects,which poses difficulties to the TEM data interpretation and inversion with conventional *** present,the IP effects break the monotony in TEM decay curves and can even cause sign reversals,and lead to the singularity and non-monotony of inversion *** is therefore important to take the IP effects into account when processing those TEM *** this paper,a new inversion method is developed to invert TEM data with IP *** Cole-Cole model was introduced to the forward modeling solver so that the IP effects can be accurately *** regularization terms were adapted to the objective function and a global optimization method,the particle swarm optimization algorithm,was used to solve the optimization problem,which weakened the singularity and non-monotony of the inversion *** data were collected in Wulong gold mine where IP effects were *** data were in-verted using the method developed in this paper,and the inversion results were able to identify the depth extension of NO.163 ore-bearing quartz veins and their associated fine-grained diorite *** global inversion method in this paper primarily solved the inversion problem of IP-affected TEM responses,and recovered the polarization parameters,which can be used as an aid to the geo-logical interpretation.

关键词： inversion IP effects TEM responses particle swarm optimization(PSO)

球囊扩张联合取栓术治疗颅内动脉狭窄合并急性闭塞的效果观察

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临床医学 2024年第9期44卷 9-11页

作者：王星淳剧新杰刘锦辉河南省驻马店市第一人民医院神内三科

目的探讨球囊扩张联合取栓术治疗颅内动脉狭窄合并急性闭塞的临床效果。方法选取2019年3月至2022年9月驻马店市第一人民医院收治的121例颅内动脉狭窄合并急性闭塞患者为研究对象。根据治疗方式分为研究组与对照组。研究组60例，采用... 详细信息

目的探讨球囊扩张联合取栓术治疗颅内动脉狭窄合并急性闭塞的临床效果。方法选取2019年3月至2022年9月驻马店市第一人民医院收治的121例颅内动脉狭窄合并急性闭塞患者为研究对象。根据治疗方式分为研究组与对照组。研究组60例，采用介入吸栓联合球囊扩张治疗；对照组61例，采用常规支架取栓治疗。比较两组患者的术后血管再通率、手术前后血流动力学指标[纤维蛋白原、血浆黏度(PV)、全血高切黏度及全血低切黏度]、治疗前后的神经功能[美国国立卫生院卒中量表(NIHSS)]。结果研究组的血管再通率(83.3%,38/61)较对照组(62.3%,50/60)高(P0.05),而术后两组的血流动力学指标均较术前改善，且研究组优于对照组(P0.05),而术后两组的神经功能均较术前改善，且研究组优于对照组(P<0.05)。结论介入吸栓联合球囊扩张对颅内动脉狭窄合并急性闭塞患者的疗效较佳，可以提高血管再通率、改善其血流动力学指标及神经功能。

关键词：介入吸栓取栓球囊扩张颅内动脉狭窄合并急性闭塞

Constitutive Behavior of the Interface between UHPC and Steel Plate without Shear Connector:From Experimental to Numerical Study

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Computer Modeling in Engineering & Sciences 2024年第8期140卷 1863-1888页

作者： Zihan wang Boshan Zhang Hui wang Qing Ai xingchun Huang School of Naval Architecture Ocean and Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China Department of Bridge Engineering Tongji UniversityShanghai200092China

The application of ultra-high performance concrete(UHPC)as a covering layer for steel bridge decks has gained widespread *** employing a connection without a shear connector between the steel plate and UHPC,namely,the sandblasted interface and the epoxy adhesive with sprinkled basalt aggregate interface,the installation cannot only be simplified but also the stress concentration resulting from the welded shear connectors can be *** study develops constitutive models for these two interfaces without shear connectors,based on the interfacial pull-off and push-out *** validation,three-point bending tests on the steel-UHPC composite plates are *** results indicated that the proposed bilinear traction-separation model for the sandblasted interface and the trapezoidal traction-separation model for the epoxy adhesive with sprinkled basalt aggregate interface can generally calibrate the interfacial ***,the utilization of the experimentally determined pure shear strength underestimates the load-carrying capacity of the composite plates in the case of three-point bending *** recalling the Mohr-Coulomb criterion,this underestimation is attributed to the enhancement of the interface shear strength by the presence of normal stress.

关键词： Cohesive zone model interfacial behavior finite element simulation UHPC steel plate

Application of TEM Based on HTS SQUID Magnetometer in Deep Geological Structure Exploration in the Baiyun Gold Deposit,NE China

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Journal of Earth Science 2021年第1期32卷 1-7页

作者： Junjie Wu Xiaodong Chen Yi Yang Qingquan Zhi xingchun wang Jie Zhang Xiaohong Deng Yi Zhao Yue Huang Institute of Geophysical and Geochemical Exploration Chinese Academy of Geological SciencesLangfang 065000China Laboratory of Geophysical Electromagnetic Probing Technologies Ministry of Land and ResourcesLangfang 065000China School of Geology Engineering and Geomatics Chang'an UniversityXi'an 710000China

Exploration of deep mineralization,particularly where the mineralization of interest is covered by a conductive overburden,is still a challenge for the conventional transient electromagnetic(TEM)method,which measures TEM response using induction coils as the ***,sensors such as fluxgate and superconductive quantum interfere device(SQUID)magnetometers can measure the B-field directly,which can provide more reliable deep information for mineralization *** this paper,we report on the research and development of our newly developed high-temperature su-perconductor(HTS)SQUID magnetometer,which is cooled by liquid nitrogen at 77 K,and its applica-tion in TEM measurement for deep exploration in a gold deposit in *** improved SQUID magnetometer version has a good performance with noise(60 fT/√Hz),slew rate(0.8 mT/S),dynamic range(100 dB),sensitivity(6.25 mV/nT),and bandwidth(DC-20 kHz).To find deep and peripheral ore in the Baiyun gold deposit located in Liaoning Province,NE China,both the SQUID magnetometer and induction coil were used for TEM data *** show that TEM can detect the distribution of local strata and the faults contained within *** also indicate that the SQUID magnetome-ter has superior response performance for response over geological targets with slower decay time when compared to the induction coil *** SQUID magnetometer is more sensitive at observing the induced-polarization effect which is closely related to the ore-controlling faults.

关键词： TEM HTS SQUID magnetometer B-field deep exploration the Baiyun gold deposit

Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice

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Science China(Life Sciences) 2017年第5期60卷 506-515页

作者： Lan Shen Yufeng Hua Yaping Fu Jian Li Qing Liu Xiaozhen Jiao Gaowei Xin Junjie wang xingchun wang Changjie Yan Kejian wang State Key Laboratory of Rice Biology China National Rice Research Institute Chinese Academy of Agricultural Sciences Hangzhou 310006 China Key Laboratory of Plant Functional Genomics Ministry of Education Yangzhou University Yangzhou 225009 China Lianyungang Academy of Agricultural Sciences Lianyungang 222006 China College of Life Sciences Shanxi Agricultural University Taigu 030801 China

The clustered regularly interspaced short palindromic repeats(CRISPR)-associated endonuclease 9(CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target genes have high mutation efficiencies in the T_0 generation. Both heterozygous and homozygous mutations of all editing genes were obtained in T_0 plants. In addition, homozygous sextuple, septuple, and octuple mutants were identified. As the abundant genotypes in T_0 transgenic plants, various phenotypes related to the editing genes were observed. The findings demonstrate the potential of the CRISPR/Cas9 system for rapid introduction of genetic diversity during crop breeding.

关键词： CRISPR/Cas9 genome editing agronomic genes rice

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辽东半岛五龙金矿外围电性特征及找矿意义

黄金科学技术 2024年第1期32卷 1-12页

作者：王兴春邱海城李建平智庆全李华武军杰邓晓红吴琼中国地质科学院地球物理地球化学勘查研究所河北廊坊065000 国家现代地质勘查工程技术研究中心河北廊坊065000 辽宁五龙黄金矿业有限责任公司辽宁丹东118012 青海省地质调查局青海西宁810000

辽东五龙金矿经过80余年的开采,矿山资源储备严重下滑,后备资源不足问题日益突出,亟需查明矿区深部和外围矿产资源。通过在v163号脉西北和东南两侧以及100号断裂南侧开展瞬变电磁法测量工作,构建了相应的二维和三维地电模型,分析了矿区... 详细信息

辽东五龙金矿经过80余年的开采,矿山资源储备严重下滑,后备资源不足问题日益突出,亟需查明矿区深部和外围矿产资源。通过在v163号脉西北和东南两侧以及100号断裂南侧开展瞬变电磁法测量工作,构建了相应的二维和三维地电模型,分析了矿区低阻异常特征;结合前人研究成果,推断出金矿成因类型和下一步找矿方向。研究结果表明:v163号脉西北方向P0剖面上低阻区是由包括163号脉西北段的细粒闪长岩脉群引起的,属于石英脉型成矿的可能性较大;100号断裂南侧三维地电模型中的低阻区域与大孤顶子金矿矿化点相对应,该低阻区向北侧深部规模增大,异常未封闭,表明该区域北侧深部具有良好的找矿前景,值得进一步开展研究工作;v163号脉东南方向的三维地电模型中,低阻异常值在数量级上明显低于前二者,推测为蚀变带引起,后经工程验证孔揭露,该区域纵向存在多层不连续的硅化蚀变岩,最大厚度为41m,蚀变类型有硅化、绢云母化、绿泥石化以及不同程度的黄铁矿化。研究表明,五龙金矿不仅存在石英脉型金矿体,还有蚀变岩型金矿体,后者可能是该矿区下一个找矿的主要方向。

关键词：瞬变电磁法石英脉型金矿蚀变岩型金矿外围找矿 v163号脉五龙金矿

Foxtail millet:nutritional and eating quality,and prospects for genetic improvement

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Frontiers of Agricultural Science and Engineering 2015年第2期2卷 124-133页

作者： Lu HE Bin ZHANG xingchun wang Hongying LI Yuanhuai HAN College of Agriculture Shanxi Agricultural UniversityTaigu 030801China Institute of Agricultural Bioengineering Shanxi Agricultural UniversityTaigu 030801China College of Biosciences Shanxi Agricultural UniversityTaigu 030801China Key Laboratory of Loess Plateau Crop Gene Resources and Germplasm Ministry of AgricultureTaiyuan 030001China Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops Shanxi Agricultural UniversityTaigu 030801China

Foxtail millet is a minor yet important crop in some areas of the world,particularly northern *** has strong adaptability to abiotic stresses,especially drought,and poor *** also has high nutritional *** millet is rich in essential amino acids,fatty acids and minerals,and is considered to be one of the most digestible and non-allergenic grains available and has significant importance for human *** foxtail millet’s ability to adapt to abiotic stresses associated with climate change,it is more important than ever to develop breeding strategies that facilitate the increasing demand for high quality grain that better satisfies *** we review research on foxtail millet quality evaluation,appearance,cooking and eating quality at the phenotypic *** review analysis of the main nutrients in foxtail millet,their relationships and the biochemical and genetic factors affecting their *** addition,we review past progress in breeding this regionally important crop,outline current status of breeding of foxtail millet,and make suggestions to improve grain quality.

关键词： foxtail millet grain quality quality evaluation breeding for quality