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A relative quantitative method to detect OCT4A gene expression by exon-junction primer and locked nucleic acid-modified probe

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第2期12卷 149-155页

作者： Jian-jun REN xing-kai meng Department of General Surgery Xuanwu Hospital Capital Medical University Beijing 100053 China Department of General Surgery Affiliated Hospital of Inner Mongolia Medical College Hohhot 010050 China

Objective: OCT4A has been known to play a critical role in the maintenance of pluripotency of embryonic stem cells. Recent research has shown that OCT4A is also expressed in partial tumor cell lines and tissues. This study is aimed to develop a real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay for relative quantitative detection of OCT4A mRNA and discrimination from OCT4B, pseudogene, and genomic contaminations. Methods: A locked nucleic acid (LNA)-modified probe was designed to discern the single base difference 352A/C to identify OCT4A mRNA. An exon-junction primer was designed to avoid false positive caused by genomic contaminations. In addition, a house keeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was measured in parallel to normalize the differences between samples and operations. Results: Experiments showed that the newly established RT-PCR assay amplified the OCT4A mRNA selectively; OCT4A analogues gave negative signals. Cell lines nTERA-2 and HepG2 showed positive results in OCT4A expression, while for HeLa and 293 cell lines, as well as primary peripheral blood mononuclear cells (PBMCs), OCT4A expression was negative. Additionally, the relative quantity of OCT4A mRNA was calculated by cycle threshold (Ct) method and house keeping gene normalization. Conclusions: This technique proved to be effective for relative quantitation of OCT4A mRNA with high specificity.

关键词： OCT4A. Locked nucleic acid (LNA). Real-time Dolvmerase chain reaction (PCR)

OSCILLATORY PROPERTIES OF THE SOLUTIONS OF NONLINEAR DELAY HYPERBOLIC DIFFERENTIAL EQUATIONS OF NEUTRAL TYPE

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Applied Mathematics and Mechanics(English Edition) 2002年第6期23卷 678-685页

作者： LIU An-ping(刘安平) HE meng-xing(何猛省) Department of Mathematics and Physics China University of GeosciencesWuhan 430074 P R China Department of Mathematics and Physics Wuhan University of TechnologyWuhan 430070 P R China

By making use of the integral inequalities and some results of the functional differential equations, oscillatory properties of solutions of certain nonlinear hyperbolic partial differential equations of neutral type with multi-delays were investigated and a series of sufficient conditions for oscillations of the equations were established. The results fully indicate that the oscillations are caused by delay and hence reveal the difference between these equations and those equations without delay.

关键词： neutral delay hyperbolic oscillation nonlinear

Bi-variable damage model for fatigue life prediction of metal components

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Acta Mechanica Sinica 2011年第3期27卷 416-425页

作者： Miao Zhang Qing-Chun meng xing Zhang Wei-Ping Hu Division 508 School of Aeronautic Science and Engineering Beihang University 100191 Beijing China

Based on the theory of continuum damage mechanics,a bi-variable damage mechanics model is developed,which,according to thermodynamics,is accessible to derivation of damage driving force,damage evolution equation and damage evolution criteria. Furthermore,damage evolution equations of time rate are established by the generalized Drucker＇s postulate. The damage evolution equation of cycle rate is obtained by integrating the time damage evolution equations,and the fatigue life prediction method for smooth specimens under repeated loading with constant strain amplitude is constructed. Likewise,for notched specimens under the repeated loading with constant strain amplitude,the fatigue life prediction method is obtained on the ground of the theory of conservative integral in damage mechanics. Thus,the material parameters in the damage evolution equation can be obtained by reference to the fatigue test results of standard specimens with stress concentration factor equal to 1,2 and 3.

关键词： Bi-variable damage model - Damage evolution equation . Life prediction - Fatigue . Damage mechanics

A Kind of Plate Laminar Cooling Strategy With High Response and Steady Precision

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Journal of Iron and Steel Research International 2010年第1期17卷 18-21,37页

作者： ZHANG Dian-hua ZHOU Na WANG Bing-xing ZHANG meng State Key Laboratory of Rolling and Automation Northeastern University Shenyang 110004 Liaoning China

After Smith Predictor being used to laminar cooling system, the control law for integral regulators is introduced. New notion of plate sample length is given for evading the delay time varying with the roll table speed. It is found that the integral algorithm is more controllable for system regulating process and has better steady state precision. Compared with the traditional control strategies, the new control law has faster response speed and higher steady state precision. The controller can be regulated in shorter time and the response is faster than that of routine controller and it can effectively eliminate the oscillatory instability. Owing to its easy realization and high stability, it has been applied to a plate mill. The accuracy of finish cooling temperature can be controlled within ± 10 ℃.

关键词： plate laminar cooling Smith Predictor time delay system

Studied microRNA gene expression in human hepatocellular carcinoma by micro RNA microarray techniques

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World Journal of Gastroenterology 2015年第44期21卷 12605-12611页

作者： Jian-Xiang Niu xing-Kai meng Jian-Jun Ren Department of General Surgery of the Affiliated Hospital of Inner Mongolia Medical University

AIM: To achieve a better understanding of the molecular mechanisms of micro RNA expression changes involved in hepatocellular ***: In this research process, patients were not treated with antivirals, immunosuppressants or immunomodulators for at least 6 mo before collecting serum. The study population was composed of 35 outpatient hepatitis B virus(HBV) cases and 12 healthy control cases from the Affiliated Hospital of Inner Mongolia Medical University(Inner Mongolia, China) from July 2013 to April 2014. The 35 HBV cases were divided into two groups: a hepatocirrhosis group with 20 cases and a liver cancer group with 15 cases. All 35 cases carried HBs Ag. The diagnostic criteria followed the European Association for the Study of the Liver 2012(EASL2012) standards. Micro RNA(mi RNA) was extracted from a control group of patients, a group with hepatocirrhosis and a group with liver cancer and its quality was analyzed using the human V2 micro RNA expression beadchip. Cluster analysis and a radar chart were then applied to the mi RNA ***: The mi RNA-qualified rate of human serum samples was 93%. The concentration of a single sample was > 200 ng/μL and the volume was > 5 μ*** mi RNA serum samples were uncontaminated by the genome. The Mann-Whitney test showed significant differences in mi RNA between each group, with a detection P-value of < 0.05. Illumina software was set up with Diff Score set to ± 13, meaning that P = *** were significant changes in mi RNA expression between the three groups. mi RNA-183 was the most up-regulated, followed by mi RNA-373. mi RNA-129 and mi RNA-188 were both strongly down-regulated and mi RNA-378 was down-regulated a small amount. The liver cancer group had greater changes, which indicated that changes in mi RNA expression levels were caused by hepatocirrhosis. The liver cancer disease course then further increased these changes. In the pentagon created by these five mi RNAs, three groups showed signif

关键词： Hepatocellular carcinoma Micro RNA Expression Micr

A methodology for measuring the preservation durability of digital formats

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Journal of Zhejiang University-Science C(Computers and Electronics) 2010年第11期11卷 872-881页

作者： Chao LI Xiao-hui ZHENG xing meng Li WANG Chun-xiao xing Research Institute of Information Technology Tsinghua University Beijing 100084 China Library of Tsinghua University Tsinghua University Beijing 100084 China

It is now widely recognized that appropriate measures are required for digital preservation to ensure that digital data can be accessed and used currently and in the *** all the risks of digital preservation,format obsolescence is one of the most *** have been several projects or initiatives dealing with the measurement method of format obsolescence risk,but there has been no mechanism to quantify the preservation risk or durability of digital formats based on a self-improving assessment model,executed with the aid of *** paper deals with a methodology for measuring the preservation durability of digital formats,especially for their risk *** method is based on a quantitative assessment model for format risk,and can shift the non-quantifiable knowledge or experiences of field experts to a machine identifiable and processible form,or 'risk scores'.Results can be recognized and communicated by computers automatically and formally,which can assist in the automatic/semi-automatic risk management for digital preservation,sharing this quantified knowledge among communities. Because technologies are changing quickly,the quantitative assessment model for risks will not be a status quo ***,also presented is a method to fine tune the quantitative assessment model for risk of formats through a self-learning and self-improving style.

关键词： Digital preservation Format obsolescence Risk assessment model Risk value

Novel invagination procedure for pancreaticojejunostomy using double purse string sutures:A technical note

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World Journal of Gastrointestinal Surgery 2023年第12期15卷 2792-2798页

作者： Jun Li He-Yuan Niu xing-Kai meng Department of Hepatobiliary Pancreatic and Splenic SurgeryThe Affiliated Hospital of Inner Mongolia Medical UniversityHohhot 010010Inner Mongolia Autonomous RegionChina

BACKGROUND Postoperative pancreatic fistula(POPF)is one of the most serious complications after pancreaticoduodenectomy(PD),and the choice of pancreaticojejunostomy(PJ)is considered a key factor affecting the occurrence of *** anastomotic methods and their modifications have been proposed,and there is no method that can completely avoid the occurrence of *** on our team’s experience in pancreatic surgery and a review of relevant literature,we describe a novel invagination procedure for PJ using double purse string sutures,which has resulted in favourable *** To describe the precise procedural steps,technical details and clinical efficacy of the novel invagination procedure for *** This study adopted a single-arm retrospective cohort study methodology,involving a total of 65 consecutive patients who underwent PD with the novel invagination procedure for PJ,including the placement of a pancreatic stent,closure of the residual pancreatic end,and two layers of purse-string *** data included age,sex,body mass index(BMI),pancreatic texture,pancreatic duct diameter,operation time,and blood *** outcomes included the operation time,blood loss,and incidence of POPF,postoperative haemorrhage,delayed gastric emptying,postoperative pulmonary infection,postoperative abdominal infection,and postoperative pulmonary *** The mean age of the patients was 59.12(±8.08)*** males and 25 females were included,and the mean BMI was 21.61 kg/m^(2)(±2.74).A total of 41.53% of patients had a pancreatic duct diameter of 3 mm or *** mean operation time was 263.83 min(±59.46),and the mean blood loss volume was 318.4 mL(±163.50).Following the surgical intervention,only three patients showed grade B POPF(4.62%),while no patients showed grade C *** patients(5/65,7.69%)were diagnosed with postoperative *** patients(6/65,9.23%)experienced delayed gastric *** patients(4/65,6.15%)developed post

关键词： Pancreatic anastomosis Pancreatojejunostomy Pancreatic fistula

Combined impact of in-phase and out-of-phase variation between the northern East Asian low and western North Pacific subtropical high on East Asian summer rainfall

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Atmospheric and Oceanic Science Letters 2017年第4期10卷 284-290页

作者： DU meng-xing LIN Zhong-Da LU Ri-Yu State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric PhysicsChinese Academy of Sciences College of Earth Science University of Chinese Academy of Sciences

East Asian summer rainfall is affected by both the continental northern East Asian low （NEAL） and the western North Pacific subtropical high （WNPSH） in the lower troposphere. This study investigates the joint effect of the two circulation factors on East Asian summer rainfall. It is found that the rainfall in East Asia behaves differently in the years with in-phase and out-of-phase variation between the NEAL and WNPSH. When the NEAL and WNPSH vary in phase, i.e. when they are both stronger, the rainfall anomaly shows a dipole pattern in East Asia and displays opposite changes between north and south of 30°N. When the two circulation factors vary out of phase, the rainfall anomaly is concentrated in the Yangtze River valley.

关键词： Northern East Asian low western North Pacificsubtropical high East Asiansummer rainfall interannualvariation

Effects of methionine treatment on storage quality and antioxidant activity of postharvest jujube fruit

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Journal of Integrative Agriculture 2023年第9期22卷 2893-2904页

作者： LIU Yao LEI xing-meng GUO Yu-xiao YAO Shi-xiang ZENG Kai-fang College of Food Science Southwest UniversityChongqing 400715P.R.China Food Storage and Logistics Research Center Southwest UniversityChongqing 400715P.R.China National Citrus Engineering Research Center Chongqing 400712P.R.China

Jujube fruits usually suffer from physiological disorders or infectious diseases during storage,leading to quality deterioration,softening,or ***,this study aims to investigate the effect of methionine soaking treatment on the postharvest jujube fruit decay rate and storage *** treatment significantly reduced the decay rate of postharvest jujube fruit and effectively maintained fruit color and titratable *** treatment effectively delayed the decrease of firmness,soluble solids,ascorbic acid,and lignin contents in jujube *** treatment reduced the content of alanine and phenylalanine,and increased the content of glycine in jujube fruit,but displayed no significant effect on total amino acid *** addition,jujube fruits in the methionine treatment group had a higher total phenolic and flavonoid content and antioxidant capacity,both in free and bound *** with the control,methionine treatment also significantly increased the content of individual phenolic acid fractions(gallic acid,vanillic acid,and syringic acid)and flavonoid fractions(catechin,epicatechin,rutin)in free form as well as individual phenolic acid fractions in bound form(gallic acid and ferulic acid)in jujube ***,this study suggested that the methionine treatment could be used as a prospective preservative to reduce the postharvest decay of jujube fruit and alleviate its nutritional quality deterioration during cold storage at 4°C.

关键词： methionine jujube decay rate sensory quality nutritional value