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Identification of Dysregulated microRNAs in Glioma Using RNA sequencing

Current Medical Science 2021年第2期41卷 356-367页

作者： Chang LIU Ying-ying GE Xiao-xun XIE Bin LUO Ning SHEN xing-sheng liao Shui-qing BI Tao XU Shao-wen XIAO Qing-mei ZHANG Department of Neurosurgery the First Affiliated HospitalGuangxi Medical UniversityNanning 530021China School of Pre-clinical Medicine Guangxi Medical UniversiryNanning 530021China Department of Stomatology the People's Hospital of Guangxi Zhuang Atutonomous RegionNanning 530021China

Glioma is the most common malignant brain tumor in central nervous *** advances in the treatment of glioma such as surgery and chemoradiotherapy,most patients are easy to relapse,resulting in adverse clinical ***,effective molecular=targeting treatment may be one of attractive strategies for glioma *** dysregulated microRNAs(miRNAs),one of the candidates of therapeutic targets,are believed to play an important role in the progression of *** this study,we aimed to examine the expression profile of miRNAs in glioma and provide a reference for glioma ***,expression profile of miRNAs in 5 normal brain tissues,5 low-grade glioma(LGG)tissues and 5 glioblastoma(GBM)tissues was detected by RNA sequencing(RNA-seq).Next,the target genes of differentially expressed miRNAs(DEmiRNAs)were predicted and then GO enrichment and KEGG pathway analysis performed by ***,10 miRNAs which were significantly up-or down-regulated both in GBM and LGG were validated by real-time quantitative PCR(qRT-PCR).RNA-seq results indicated a number of DEmiRNAs in *** were 64 up-regulated miRNAs and 17 down-regulated miRNAs n LGG,and 181 up-regulated miRNAs and 124 down-regulated miRNAs in GBM,*** analysis showed that the target genes of these DEmiRNAs were enriched in various biological processes and signaling pathways such as cell metabolic and developmental *** DEmiRNAs were further confirmed by ***-10b-5p,miRNA-92b-3p and miRNA-455-5p were significantly up-regulated in both GBM and LGG;while miRNA-542-3p was significantly up-regulated in LGG;miRNA-184 and miRNA-206 were significantly down-regulated in both GBM and LGG;miRNA-766-5p and miRNA-1-3p were significantly down-regulated in *** subject of our study demonstrated several dysregulated miRNAs may serve as a potential therapeutic target for gl ioma.

关键词： glioma microRNA RNA-sequencing

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Registration of intervention trials of Traditional Chinese Medicine for four neurological diseases on Chinese Clinical Trial Registry and Clinical ***:a narrative review

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Journal of Traditional Chinese Medicine 2022年第1期42卷 148-153页

作者： WEI Jingjing GUO Rongjuan FU Guojing LIANG Xiao XU Zhenmin JIA Min ZENG Zixiu DU Wanqing JIAO Weiwei SUN Linjuan LIU Hongmei GUO Chunli TONG Chenguang ZHANG Yunling liao xing Graduate school Beijing University of Chinese MedicineBeijing 10029China Center for Evidence Based Chinese Medicine Institute of Basic Research in Clinical MedicineChina Academy of Chinese Medical SciencesBeijing 100700China Department of Neurology Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing 100091China Department of Neurology Dongfang HospitalBeijing University of Chinese MedicineBeijing 100078China

OBJECTIVE:To analyze the current status of clinical trial registration of Traditional Chinese Medicine(TCM)for the treatment of neurological ***:Interventional clinical trials of TCM treatment for ischemic stroke,hemorrhagic stroke,vascular cognitive impairment,tension-type headache before September 22,2020 on the platform of Chinese Clinical Trial Registry(Chi CTR),and Clinical *** were *** researchers independently selected the literature and extracted ***:A total of 180 interventional clinical trials were included for *** of 180 trials,127 were from Chi CTR and 53 from Clinical *** countries primary sponsoring the included trials were China(176,97.8%),and the common categories of primary sponsors were hospital(131,72.8%).Among the study design,the largest proportion of allocation was randomized(172,95.6%),interventional model assignment was parallel(163,90.6%),masking was double blind 49(27.2%),and the sample size was≤400(144,80.0%).The trials were most carried out at a single center(102,56.7%).Among the included studies,112(62.2%)registered on Chi CTR attached the ethical approval *** terms of trial stages,50(27.7%)studies were in phaseⅣ.The mostly used intervention was Chinese herbal medicines(99,55%),acupuncture(68,37.8%)was the *** searching the registration number on China National Knowledge Infrastructure Database and Pub Med,38(21.1%)registered trials were published,including 25 protocol studies and 14 research results with one(NCT02275949)published both the protocol and the ***:Irregular and inadequate reporting,untimely update and publication,insufficient information on traditional medicine unique characteristics,and lack of international collaborations are the problems existing in the interventional clinical registration trials of traditional medicine treatment on neurological *** efforts need to be made from the above aspects to standardize and improve th

关键词： medicine Chinese traditional ischemic stroke cognitive dysfunction tension-type headache hemorrhagic stroke Chinese Clinical Trial Registry Clinical Trials.gov critical appraisal

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SPAD chorophyll meter was suitable in nitrogen quick diagnosis,yield forecast and fertilization recommendation in rapeseed(Brassica napus L. )

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Oil Crop Science 2016年第2期1卷 51-59页

作者： Changbing YU Yinshui LI Lihua XIE Xiaojia HU Zhi CHE Xiangsheng liao xing liao Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture

Nitrogen （N） is one of the most important nutrients for oilseed rape （Brassica napus L . ） , but there is a lack of quick diagnosis and recommended fertilization. Three field experiments were con-ducted to seek the best diagnostic method and establish a fertilizer rate recommendation model. For ex-periment 1, six treatments as application N 0,90,180,240,300 and 400kg ha -1 were designed, and the general N nutrition diagnosis method as SPAD -502 chlorophyll meter, RQflex plus 10 nitrate reflectrom- eter and GreenSeeker - 505 spectrometer were used at eight leaf stage, tenth leaf stage and bolting stage of rape to determine the suitable nitrogen rapid diagnosis method and establish the model of yield forecast and N rate recommendation as topdressing. The experiment 2 was designed to validate the veracity of yield forecast model obtained from experiment 1. The experiment 3 was designed to test the accuracy of N rate recommendation model come from experiment 1. The results indicated that there is a preferable inter-relation between N application rates and rape yield for experiment 1 （ y = - 0. 0176x2 ＋ 11. 136x ＋797. 88 ,R2 =0. 9905 ＊＊） , which conform to the performance of yield variation with N application levels as shortage, appropriate and excessive rapeseed. For three diagnosis methods, nitrate reflectrometer could reflect rape N nutrition status to some extent, but which influenced by rapeseed physiological char-acteristics such as lower biomass at seedling stage and luxury absorption of N at bolting stage, and the re-liability and stability of diagnosis were inferior. Spectrometer is suitable for quick N nutrition diagnosis at bolting stage, but the stage of topdressing is too late to meet the demand of N support. SPAD meter could more suitable in quick N diagnosis of oilseed rapes since it could reflect rape N nutrition preferable, and it has some advantages such as the strong stability, easy measuring and lower cost in detection. By SPAD meter, mode

关键词： oilseed rape ( Brassica napus L. ) quick nitrogen diagnosis nitrate reflectrometer SPAD chorophyll meter spectrometer

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3-4GHz Bandpass Filters Using Dielectric-Filled Stepped impedance resonators

3-4GHz Bandpass Filters Using Dielectric-Filled Stepped impe...

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2000 2nd International Conference on Microwave and Millimeter Wave Technology

作者： Zhao Hua-De liao xing-Hua Wang Rong Southwest institute of Applied Magnetics of China

Describes the principle ofλ/2 dielectric-filled stepped impedance Resonators and the Design formulae are *** filters of 3-4GHz Band Using theλ/2 dielectric-filled stepped impedance Resonators provide low insertion loss(3.5dB) small passband ripple (±0.5),high stop-band(27dB),small size,small weight and high stability.

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Primary early-stage intestinal and colonic non-Hodgkin's lymphoma: Clinical features, management, and outcome of 37 patients

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World Journal of Gastroenterology 2005年第37期11卷 5905-5909页

作者： Shu-Lian Wang Zhong-xing liao Xin-Fan Liu Zi-Hao Yu Da-Zhong Gu Tu-Nan Qian Yong-Wen Song Jing Jin Wei-Hu Wang Ye-Xiong Li Department of Radiation Oncology Cancer HospitalPeking Union Medical College and Chinese Academy of Medical Sciences Beijing 100021 China Department of Radiation Oncology The University of Texas MD Anderson Cancer Center Houston 77030 TX USA

AIM： To analyze the clinical features, management, and outcome of treatment of patients with primary intestinal and colonic non-Hodgkin＇s lymphoma （PICL）. METHODS： A retrospective study was performed in 37 patients with early-stage PICL who were treated in our hospital from 1958 to 1998. Their clinical features, management, and outcome were assessed. Prognostic factors for survival were analyzed by univariate analysis using the Kaplan-Meier product-limit method and log-rank test. RESULTS： Twenty-five patients presented with Ann Arbor stage Ⅰ PICL and 12 with Ann Arbor stage Ⅱ PICL. Thirty-five patients underwent surgery （including 31 with complete resection）, 22 received postoperative chemotherapy or radiotherapy or both. Two patients with rectal tumors underwent biopsy and chemotherapy with or without radiotherapy. The 5- and 10-year overall survival （OS） rates were 51.9% and 44.5%. The corresponding diseasefree survival （DIS） rates were 42.4% and 37.7%. In univariate analysis, multiple-modality treatment was associated with a better DFS rate compared to single treatment （P= 0.001）. While age, tumor size, tumor site, stage, histology, or extent of surgery were not associated with OS and DFS, use of adjuvant chemotherapy significantly improved DFS （P = 0.031） for the 31 patients who underwent complete resection. Additional radiotherapy combined with chemotherapy led to a longer survival than chemotherapy alone in six patients with gross residual disease after surgery or ***： Combined surgery and chemotherapy is recommended for treatment of patients with PICL, Additional radiotherapy is needed to improve the outcome of patients who have gross residual disease after surgery.

关键词： Intestinal neoplasm Colonic neoplasm Non-Hodgkin's lymphoma Surgery Chemotherapy Radiotherapy

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Gene Expression Profile Identifies LncRNA AL355974.3 As a Potential Glioma Biomarker

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Current Medical Science 2024年第5期44卷 1047-1057页

作者： Hao-tian LU Yu-yang CHEN Yong-jia YU xing-sheng liao Hui LIANG Lun LIANG Pan-lin MO Xin-kai HUANG Shuai DING Chang LIU Da-qin FENG Department of Neurosurgery The First Affiliated HospitalGuangxi Medical UniversityNanning530021China

Objective Glioma is a central nervous system tumor arising from glial *** significant advances in diagnosis and treatment,most patients with high-grade gliomas have a poor *** studies have shown that long noncoding RNAs(lncRNAs)may play important roles in the development,progression and treatment of many tumors,including *** targeted therapy may be a new direction for the adjuvant treatment of ***,we hope that by studying differentially expressed lncRNAs(DElncRNAs)in glioma,we can discover lncRNAs that can serve as biomarkers for glioma and provide better therapeutic modalities for glioma *** First,the expression of lncRNAs in 5 normal brain(NB)tissues and 10 glioma tissues was examined by RNA sequencing(RNA-seq).Next,we performed Kaplan-Meier analysis of data from The Cancer Genome Atlas(TCGA)database to assess the prognostic value of these ***,functional analysis of the DElncRNAs was performed by means of Gene Ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway *** RNA sequencing analysis revealed 85 upregulated miRNAs and 71 downregulated lncRNAs in low-grade glioma(LGG)and 50 upregulated lncRNAs and 70 downregulated lncRNAs in glioblastoma(GBM).Among them,AL355974.3 was the most upregulated ***00632 was the most downregulated ***,LGG patients with higher AL355974.3 expression had worse overall survival according to Kaplan-Meier analysis of the TCGA ***,bioinformatics analysis revealed that the target genes of these DElncRNAs were enriched in various biological processes and signaling pathways,such as cell metabolic and developmental *** Our findings provide evidence that AL355974.3 may be a new biomarker for glioma.

关键词： glioma long noncoding RNAs biomarker RNA sequencing

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Purified isolation of vacuoles from Sedum alfredii leaf-derived protoplasts

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第1期18卷 85-88页

作者： Xiao-yu GAO xing-cheng liao Ruo-lai WU Ting LIU Hai-xing WANG Ling-li LU MOE Key Laboratory of Environment Remediation and Ecological Health College of Environmental & Resource SciencesZhejiang University Hangzhou 310058 China

This study aims to develop a method for isolating and purifying protoplasts/vacuoles from fresh leaves of the Cd hyperaccumulator plant species, Sedum alfredii. The results revealed that preheating cellulase and macerozyme at 50 ℃ for 5 rain significantly accel- erated the cell wall degradation. For the most optimal conditions for mesophyll protoplast isolation, the mixture of fresh leaves and cell lysates was followed by a 2-h-long vibration. The protoplast lysate for vacu- ole isolation was diluted, and 0.675 mmol/L was identi- fied as the most appropriate 3-[（3-cholamidopropyl） dimethylammonio]- 1 -propanesulfonic acid （CHAPS） level, in which S. alfredii large vacuoles are charac- terized by a high metal and malic acid content.

关键词：超积累植物东南景天原生质体液泡提取与纯化

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Research on the Peanut Leaf Etiolation Prevention and Film Mulching Effect in Hubei Province

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Asian Agricultural Research 2014年第1期6卷 91-94页

作者： Changbing YU Yinshui LI Lihua XIE Xiaojia HU Boshou liao Fang CHEN xing liao Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture Wuhan Botanical Garden Chinese Academy of Sciences

The possible nutrient problems existing in leaf etiolation,the influence of film mulching on the yield of peanut and benefits of balanced fertilization were analyzed. The results showed that the deficiency of nutrient elements was not main limiting factor in leaf etiolation of peanut in Hong'an. Deep trench,film mulching,liming and organic manure could be used to prevent leaf etiolation. The film mulching could increase the yield of peanut by improving the rate of germination and seedling,the number of branches and full pod,and it was beneficial to playing the effect of balanced fertilization.

关键词： Peanut Leaf etiolation Film mulching Balanced fert

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以创新人才培养为导向的高校研究型教学模式构建

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黑龙江高教研究 2022年第4期40卷 141-146页

作者：廖兴湖南工业大学文学与新闻传播学院湖南株洲412007

基于我国高校创新人才培养的时代发展诉求,借鉴野中郁次郎的知识创新模型,结合其所蕴含的知识观、教学观和师生观,通过构建起以培育知识创新精神为灵魂、以形成知识创新思维为重点、以生产和生活实践领域为教学情境、以个体创新与群体... 详细信息

基于我国高校创新人才培养的时代发展诉求,借鉴野中郁次郎的知识创新模型,结合其所蕴含的知识观、教学观和师生观,通过构建起以培育知识创新精神为灵魂、以形成知识创新思维为重点、以生产和生活实践领域为教学情境、以个体创新与群体创新相依相促的平等和谐为教学氛围的高校研究型教学模式,为高校创新型人才的培养提供可能的借鉴意义。

关键词：创新型人才 SECI模型高校研究型教学模式

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水体景观可视度对住宅地价溢出效应研究——以深圳市为例

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北京大学学报（自然科学版） 2018年第1期54卷 197-206页

作者：吴健生朱洁廖星北京大学深圳研究生院城市规划与设计学院城市人居环境科学与技术重点实验室深圳518055 北京大学城市与环境学院地表过程与模拟教育部重点实验室北京100871

为了研究"招拍挂"机制下水体景观可视度对地价的影响,使政府认识水体景观的意义,也为市场参与者做出理性竞买决策提供参考,基于深圳市土地房产交易中心的住宅土地交易数据、Google Earth和百度地图POI数据以及Landsat数据,使用地理加权... 详细信息

为了研究"招拍挂"机制下水体景观可视度对地价的影响,使政府认识水体景观的意义,也为市场参与者做出理性竞买决策提供参考,基于深圳市土地房产交易中心的住宅土地交易数据、Google Earth和百度地图POI数据以及Landsat数据,使用地理加权回归模型,定量分析水体景观对住宅地价的影响,讨论水体景观可视度溢价的区位差异。结果表明,海域可视面积每增加1万平方米,住宅地价溢价为0.4~0.79元/m^2,增加0.005%~0.009%;水库可视面积等级每上升1级,对于地价的贡献为1176~1991元/m^2,增加13.490%~22.838%;二级河网可视长度每增加100 m,溢价为122~503元/m^2,增加1.399%~5.770%。水体景观可视度对深圳市住宅地价的影响呈现显著的空间异质性,二级河网可视长度的溢价从东至西逐步下降;海域可视面积溢价峰值区在福田区、罗湖区和盐田区,并向东西方向递减;水库可视面积等级对地价的溢价峰值主要在福田区、罗湖区、龙华和龙岗,并向东西方向递减。研究结果丰富了地价区位影响因素理论,并为景观可视度量化提供详细的参考资料。

关键词：住宅地价水体景观可视度 GWR 溢价效应

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