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Journal of Environmental Sciences 2023年第11期133卷 23-36页

作者： Yating du Guangshen Shang Junjie Zhai xing Wang College of Resources and Environmental Sciences China Agricultural UniversityBeijing 100193China Beijing Key Laboratory of Biodiversity and Organic Farming Beijing 100193China Organic Recycling Research Institute(Suzhou) China Agricultural UniversitySuzhou 215100China

With increasing production of kitchen waste,cooking oil gradually enters the soil,where it can negatively affect soil fauna.In this study,we explored the effects of soybean oil on the survival,growth,reproduction,tissue structure,biochemical responses,mRNA expression,and gut microbiome of earthworms(Eisenia fetida).The median lethal concentration of soybean oil was found to be 15.59%.Earthworm growth and reproduction were significantly inhibited following exposure to a sublethal concentration of soybean oil(1/3 LC50,5.2%).The activity of the antioxidant enzymes total superoxide dismutase(T-SOD),peroxidase(POD),and catalase(CAT)were affected under soybean oil exposure.The glutathione(GSH)content decreased significantly,whereas that of the lipid peroxide malondialdehyde(MDA)increased significantly after soybean oil exposure.mRNA expression levels of the SOD,metallothionein(MT),lysenin and lysozyme were significantly upregulated.The abundance of Bacteroides species,which are related to mineral oil repair,and Muribaculaceae species,which are related to immune regulation,increased within the earthworm intestine.These results indicate that soybean oil waste is toxic to earthworms.Thus,earthworms deployed defense mechanisms involving antioxidant system and gut microbiota for protection against soybean oil exposure-induced stress.

关键词： Gene expression Gut microbiota Eisenia fetida Soybean oil Reproduction

NORHA,a novel follicular atresia-related lncRNA,promotes porcine granulosa cell apoptosis via the miR-183-96-182 cluster and FoxO1 axis

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Journal of Animal Science and Biotechnology 2022年第2期13卷 441-457页

作者： Wang Yao Zengxiang Pan Xing DU Jinbi Zhang Honglin Liu Qifa Li College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China

Background:Follicular atresia has been shown to be strongly associated with a low follicle utilization rate and female infertility,which are regulated by many factors such as microRNAs(miRNAs),which constitute a class of noncoding RNAs(ncRNAs).However,little is known about long noncoding RNAs(lncRNAs),which constitute another ncRNA family that regulate follicular atresia.Results:A total of 77 differentially expressed lncRNAs,including 67 upregulated and 10 downregulated lncRNAs,were identified in early atretic follicles compared to healthy follicles by RNA-Sequencing.We characterized a noncoding RNA that was highly expressed in atretic follicles(NORHA).As an intergenic lncRNA,NORHA was one of the upregulated lncRNAs identified in the atretic follicles.To determine NORHA function,RT-PCR,flow cytometry and western blotting were performed,and the results showed that NORHA was involved in follicular atresia by influencing GC apoptosis with or without oxidative stress.To determine the mechanism of action,bioinformatics analysis,luciferase reporter assay and RNA immunoprecipitation assay were performed,and the results showed that NORHA acted as a‘sponge’,that directly bound to the miR-183-96-182 cluster,and thus prevented its targeted inhibition of FoxO1,a major sensor and effector of oxidative stress.Conclusions:We provide a comprehensive perspective of lncRNA regulation of follicular atresia,and demonstrate that NORHA,a novel lncRNA related to follicular atresia,induces GC apoptosis by influencing the activities of the miR-183-96-182 cluster and FoxO1 axis.

关键词： Follicular atresia Granulosa cell apoptosis ncRNA NORHA Oxidative stress

Palladium-catalysed diastereodivergent inverse- electron-demand oxa-Diels–Alder reactions of in situ formed cyclopentadienones via ligand- control

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Organic Chemistry Frontiers 2022年第5期9卷 1364-1369页

作者： xing-xing Yang xing-Liang Zhao Qin Ouyang Wei du Ying-Chun Chen Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province and Sichuan Research Center for Drug Precision Industrial TechnologyWest China School of PharmacySichuan UniversityChengdu 610041China College of Pharmacy Third Military Medical UniversityShapingbaChongqing 400038China

Here we report Pd(0)-catalysed asymmetric inverse-electron-demand oxa-Diels–Alder reactions between the carbonates of 4-hydroxy-2-cyclopentenones andα-cyano chalcones by in situ generating η^(2)-Pd(0)-cyclopentadienone complexes as HOMO-raised dienophiles,and diastereodivergent synthesis could be realised by employing a bifunctional monophosphine ligand from a chiral aminoalcohol or a proline-derived bisphosphine ligand.

关键词： inverse Palladium stereo

The mevalonate pathway promotes the metastasis of osteosarcoma by regulating YAP 1 activity via RhoA

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Genes & Diseases 2022年第3期9卷 741-752页

作者： Xing DU Yunsheng Ou Muzi Zhang Kai Li Wei Huang Dianming Jiang Department of Orthopedics The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China

Osteosarcoma is the most common malignant bone tumour,and the metastasis of osteosarcoma is an important cause of death.Evidence has shown that the mevalonate pathway is highly activated and is expected to be a new target for tumour therapy.In this study,we investigated the effect of mevalonate signalling on osteosarcoma metastasis and its molecular mechanism.First,we found that the key rate-limiting enzyme of mevalonate signalling,3-hydroxy-3-methylglutaryl-CoA reductase(HMGCR),was highly expressed in osteosarcoma cells,and inhibition of HMGCR with simvastatin significantly inhibited the motility of 143B cells.Next,we found that YAP1 activity was significantly upregulated in osteosarcoma cells and that YAP1 knockdown inhibited the motility of 143B cells.We also found that the mevalonate pathway regulated the motility of 143B cells by modulating YAP1 phosphorylation and cellular localization.Moreover,we found that the activity of YAP1 was regulated by the mevalonate pathway by modulating the cell membrane localization of RhoA.Finally,we demonstrated that inhibition of the mevalonate pathway notably reduced the lung metastasis of 143B cells,as reflected by the decreased incidence and number of metastatic nodules and the increased survival time of the nude mice.Taken together,our findings suggest that the mevalonate pathway can promote the metastasis of osteosarcoma by activating YAP1 via RhoA.Inhibition of the mevalonate pathway may be a promising therapeutic strategy for osteosarcoma metastasis.

关键词： Metastasis Mevalonate pathway Osteosarcoma RhoA YAP1

Sparse Modal Decomposition Method Addressing Underdetermined Vortex-Induced Vibration Reconstruction Problem for Marine Risers

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China Ocean Engineering 2024年第2期38卷 285-296页

作者： du Zun-feng ZHU Hai-ming YU Jian-xing State Key Laboratory of Hydraulic Engineering Intelligent Construction and Operation School of Civil EngineeringTianjin UniversityTianjin 300350China

When investigating the vortex-induced vibration(VIV)of marine risers,extrapolating the dynamic response on the entire length based on limited sensor measurements is a crucial step in both laboratory experiments and fatigue monitoring of real risers.The problem is conventionally solved using the modal decomposition method,based on the principle that the response can be approximated by a weighted sum of limited vibration modes.However,the method is not valid when the problem is underdetermined,i.e.,the number of unknown mode weights is more than the number of known measurements.This study proposed a sparse modal decomposition method based on the compressed sensing theory and the Compressive Sampling Matching Pursuit(Co Sa MP)algorithm,exploiting the sparsity of VIV in the modal space.In the validation study based on high-order VIV experiment data,the proposed method successfully reconstructed the response using only seven acceleration measurements when the conventional methods failed.A primary advantage of the proposed method is that it offers a completely data-driven approach for the underdetermined VIV reconstruction problem,which is more favorable than existing model-dependent solutions for many practical applications such as riser structural health monitoring.

关键词： motion reconstruction vortex-induced vibration(VIV) marine riser modal decomposition method compressed sensing

Electro-conductive crosslinked polyaniline/carbon nanotube nanofiltration membrane for electro-enhanced removal of bisphenol A

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Frontiers of Environmental Science & Engineering 2023年第5期17卷 81-92页

作者： Haiguang Zhang Lei du Jiajian xing Gaoliang Wei Xie Quan Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education) School of Environmental Science and TechnologyDalian University of TechnologyDalian 116024China

Nanofiltration(NF)has attracted increasing attention for wastewater treatment and potable water purification.However,the high-efficiency removal of micropollutants by NF membranes is a critical challenge.Owing to the adsorption and subsequent diffusion,some weakly charged or uncharged micropollutants,such as bisphenol A(BPA),can pass through NF membranes,resulting in low removal rates.Herein,an effective strategy is proposed to enhance the BPA removal efficiency of a crosslinked polyaniline/carbon nanotube NF membrane by coupling the membrane with electro-assistance.The membrane exhibited a 31.9%removal rate for 5 mg/L BPA with a permeance of 6.8 L/(m2·h·bar),while the removal rate was significantly improved to 98.1%after applying a voltage of 2.0 V to the membrane.Furthermore,when BPA coexisted with humic acid,the membrane maintained 94%removal of total organic carbon and nearly 100%removal of BPA at 2.0 V over the entire filtration period.Compared to continuous voltage applied to the membrane,an intermittent voltage(2.0 V for 0.5 h with an interval of 3.5 h)could achieve comparable BPA removal efficiency,because of the combined effect of membrane adsorption and subsequent electrochemical oxidation.Density functional theory calculations and BPA oxidation process analyses suggested that BPA was adsorbed by two main interactions:π–πand hydrogen-bond interactions.The adsorbed BPA was further electro-degraded into small organic acids or mineralized to CO_(2) and H2O.This work demonstrates that NF membranes coupled with electro-assistance are feasible for improving the removal of weakly charged or uncharged micropollutants.

关键词： Nanofiltration Membrane Electro-assistance Adsorption Bisphenol A

miR‑423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis

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Journal of Animal Science and Biotechnology 2024年第2期15卷 632-644页

作者： Yuqi Li Zhuofan Zhang Siqi Wang Xing DU Qifa Li College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China

Background Atresia and degeneration,a follicular developmental fate that reduces female fertility and is triggered by granulosa cell(GC)apoptosis,have been induced by dozens of miRNAs.Here,we report a miRNA,miR-423,that inhibits the initiation of follicular atresia(FA),and early apoptosis of GCs.Results We showed that miR-423 was down-regulated during sow FA,and its levels in follicles were negatively correlated with the GC density and the P4/E2 ratio in the follicular fluid in vivo.The in vitro gain-of-function experiments revealed that miR-423 suppresses cell apoptosis,especially early apoptosis in GCs.Mechanically speaking,the miR-423 targets and interacts with the 3’-UTR of the porcine SMAD7 gene,which encodes an apoptosis-inducing factor in GCs,and represses its expression and pro-apoptotic function.Interestingly,FA and the GC apoptosis-related lncRNA NORHA was demonstrated as a ceRNA of miR-423.Additionally,we showed that a single base deletion/insertion in the miR-423 promoter is significantly associated with the number of stillbirths(NSB)trait of sows.Conclusion These results demonstrate that miR-423 is a small molecule for inhibiting FA initiation and GC early apoptosis,suggesting that treating with miR-423 may be a novel approach for inhibiting FA initiation and improving female fertility.

关键词： Granulosa cell apoptosis miR-423 NORHA SMAD7 Sow fertility traits

Graphene-based hemostatic sponge

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Chinese Chemical Letters 2022年第2期33卷 703-713页

作者： Bingxin Wu Fanglin du Wenjing A Guofeng Li xing Wang State Key Laboratory of Organic-Inorganic Composites Beijing Laboratory of Biomedical MaterialsBeijing University of Chemical Technology Beijing100029China

Graphene-based sponge is a novel hemostatic material prepared by chemical cross-link of graphene oxide. It has a fast fluid absorption capacity to quickly absorb blood from wounds, activate clotting pathways, and achieve rapid hemostasis. In addition, graphene-based sponge is also a good platform carrier.It can be prepared by organic cross-linking, compounding with inorganic clay, and adding bioactive factors to enhance coagulation stimulation. By these methods, the hemostatic performance of the sponge is further improved, which shows great potential for application in the field of trauma hemostasis. This article reviews the research progress of graphene-based sponges from three different preparation strategies(organic cross-linking, inorganic compounding and adding bioactive factor), summarizes their hemostatic mechanisms, and prospects the development of graphene-based hemostatic sponges.

关键词： Graphene Hemostatic sponge Composite materials Synergistic effect Coagulation stimulation

Definition of fine roots on the basis of the root anatomy, diameter,and branch orders of one-year old Fraxinus mandshurica seedlings

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Journal of Forestry Research 2018年第5期29卷 1321-1327页

作者： Xinzhu du xing Wei School of Forestry Northeast Forestry UniversityHarbin 150040 People's Republic of China Northeast Native Tree Species Extension Center Harbin 150040 People's Republic of China

Fine roots are important in root absorption of nutrient and water,and in root turnover.Accurate definition of fine roots is a prerequisite to improved estimation of the physiological and ecological functions of forest ecosystems.Root development and physiological functions are reflections of root anatomical structure.In this study,the anatomical structures of different root orders were analyzed by examining paraffin sections of one-year old Fraxinus mandshurica seedlings.One-year-old F.mandshurica seedlings had over five root orders.The root anatomical structures of all orders showed more differences.First and second order roots consisted of four sections： the epidermis,cortex,pericycle,and vascular bundles.Fourth and fifth order roots were mainly composed of the skin and peripheral vascular bundles（including the xylem and phloem）.Third order roots had root epidermal and cortical structures,but the quantity and integrity of the cortical cells were inferior to those of the first and second order roots,and superior to those of the fourth and fifth order roots.All the first and second order roots and some third order roots with discontinuous cork layer（〈0.4 mm in diameter）,but not the fourth and fifth order roots,were the fine roots of one-year old F.mandshurica seedlings.Although they had similar diameters,different portions of root systems had different anatomical structures and therefore,vary in capacity to absorb water and nutrients.Fine roots were accurately defined by root diameter,branch orders,and anatomical structural features of one-year old F.mandshurica seedlings.

关键词： Fine root Root order Fraxinus mandshurica Anatomical structure

The Pathway of China’s Integrated Delivery System:Based on the Analysis of the Medical Consortium Policies

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Current Medical Science 2022年第6期42卷 1164-1171页

作者： Huan GAO xing-li du Jia-zhi LIAO Li XIANG School of Medicine and Health Management Huazhong University of Science and TechnologyWuhan430030China Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

With the deepening of China’s health-care reform,an integrated delivery system has gradually emerged with the function of improving the efficiency of the health-care delivery system.For China’s integrated delivery system,a medical consortium plays an important role in integrating public hospitals and primary care facilities.The first medical consortium policy issued after the COVID-19 pandemic apparently placed hope on accelerating the implementation of a medical consortium and tiered health-care delivery system.This paper illustrates the possible future pathway of China’s medical consortium through retrospection of the 10-year process,changes of the series of policies,and characteristics of the policy issued in 2020.We considered that a fully integrated medical consortium would be a major phenomenon in China's medical industry,which would lead to the formation of a dualistic care pattern in China.

关键词： health-care reform integrated delivery system medical consortium tiered health-care delivery system health policy