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National Science Review 2020年第5期7卷 835-837页

作者： Changyang Zhou xinde hu Cheng Tang Wenjia Liu Shaoran Wang Yingsi Zhou Qimeng Zhao Qiyu Bo Linyu Shi Xiaodong Sun Haibo Zhou hui Yang Institute of Neuroscience State Key Laboratory of Neuroscience Key Laboratory of Primate Neurobiology CAS Center for Excellence in Brain Science and Intelligence Technology Shanghai Research Center for Brain Science and Brain-Inspired Intelligence Shanghai Institutes for Biological Sciences Chinese Academy of Sciences College of Life Sciences University of Chinese Academy of Sciences Department of Ophthalmology Shanghai General Hospital (Shanghai First People’s Hospital)Shanghai Jiao Tong University School of MedicineShanghai Key Laboratory of Fundus Diseases

RNA-targeting CRISPR system Cas13 offers an efficient approach for manipulating RNA transcripts in vitro. In this perspective, we provide a proof-of-concept demonstration that Cas13-mediated Vegfa knockdown in vivo co... 详细信息

关键词： AAV AMD RNA CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration

PI3K Regulatory Subunit P85 Regulates Mouse Cortical Development

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PI3K Regulatory Subunit P85 Regulates Mouse Cortical Develop...

Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice

中国神经科学学会第十二届全国学术会议

作者： Xinran Cheng Kaikai Li Meng Meng Liu xinde hu Mingrui Xu Runchuan Yan Shanting Zhao College of Veterinary Medicine Northwest A&F University Institute of Neuroscience State Key Laboratory of Neuroscience Key Laboratory of Primate Neurobiology CAS Center for Excellence in Brain Science and Intelligence Technology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences College of Food Science and Engineering Northwest A&F University

Mouse cortical development requires an exquisite regulation of neurons production and neuronal migration. Neurons production is mainly depend on the balance between proliferation and differentiation of neural progenitor cells（NPCs） and neural stem cells（NSCs）, and after that, projection neurons migrate from the ventricular zone（VZ） to the cortical plate（CP）, guided by radial glial cells（RGCs） with a transformation between bipolar and multipolar morphology. Previous studies have demonstrated that the PI3 K-Akt-m TOR signaling pathway plays a critical role in brain development. However, the function of p85 subunit in cortical development is still unclear. In this study, through primary neuron culture and in utero electroporation combined with immunohistochemistry and microscope image, we analyzed the function of p85 in mouse cortical development. Our results showed that overexpression of p85 inhibited NPCs proliferation. Also, overexpression of p85 perturbed the transition from intermediate neuronal progenitors（INPs） to projection neurons, and restrained neuronal ***’s more, we found that overexpression of p85 impaired cortical neuronal migration by affecting neuronal morphology. However, overexpression of p85 had impact on the final destination of cortical neurons, but did not affect ultimate fate decision of them. These findings indicated that the p85 subunit plays an essential role in architectural and functional integrity of the mouse cerebral cortex.

关键词： p85 subunit in utero electroporation neuronal migration proliferation differentiation

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National Science Review 2020年第1期7卷 92-101页

作者： Jin-Jing Li Xiang Lin Cheng Tang Ying-Qian Lu xinde hu Erwei Zuo He Li Wenqin Ying Yidi Sun Lu-Lu Lai Hai-Zhu Chen Xin-Xin Guo Qi-Jie Zhang Shuang Wu Changyang Zhou Xiaowen Shen Qifang Wang Min-Ting Lin Li-Xiang Ma Ning Wang Adrian R.Krainer Linyu Shi hui Yang Wan-Jin Chen Department of Neurology and Institute of Neurology The First Affiliated Hospital of Fujian Medical University Fujian Key Laboratory of Molecular Neurology Fujian Medical University Institute of Neuroscience State Key Laboratory of Neuroscience Key Laboratory of Primate Neurobiology CAS Center for Excellence in Brain Science and Intelligence Technology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Key Lab of Computational Biology CAS-MPG Partner Institute for Computational Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Department of Anatomy Histology & Embryology Shanghai Medical College Fudan University Cold Spring Harbor Laboratory Cold Spring Harbor

We here report a genome-editing strategy to correct spinal muscular atrophy(SMA). Rather than directly targeting the pathogenic exonic mutations, our strategy employed Cas9 and guide-sg RNA for the targeted disruption of intronic splicing-regulatory elements. We disrupted intronic splicing silencers(ISSs, including ISS-N1 and ISS + 100) of survival motor neuron(SMN) 2, a key modifier gene of SMA, to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs. Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration. Furthermore, co-injection of Cas9 mRNA from Streptococcus pyogenes(SpCas9) or Cas9 from Staphylococcus aureus(SaCas9) alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56% and 100% of severe SMA transgenic mice(Smn-/-, SMN2tg/-).The median survival of the resulting mice was extended to >400 days. Collectively, our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases.

关键词： spinal muscular atrophy SMN2 splicing-regulatory elements CRISPR/Cas9 germline correction

The Toxic Influence of Paraquat on Hippocampal Neurogenesis in Adult Mice

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The Toxic Influence of Paraquat on Hippocampal Neurogenesis ...

中国神经科学学会第十二届全国学术会议

作者： Kaikai Li Xinran Cheng Jinhua Jiang Jiutao Wang Jiongfang Xie xinde hu Yingxue huang Lingzhen Song Mengmeng Liu Leiming Cai Liezhong Chen Shanting Zhao College of Veterinary Medicine Northwest A&F University Zhejiang Academy of Agricultural Science China-US (Henan) Hormel Cancer Institute

Objective Paraquat, a fast-acting non-selective contact herbicide, is considered an etiological factor related to Parkinson’s disease. This study investigated its effects on hippocampal neurogenesis and cognition in adult mice as well as possible mechanisms for the effects. Methods We administered paraquat（1.25 mg/kg, intraperitoneal injection, i.p.） and an equal volume of normal saline for 3 weeks to adult male C57 BL/6 J mice. Results The results showed that hippocampus-dependent spatial learning and memory was significantly impaired in paraquat-treated mice. Moreover, paraquat administration inhibited the proliferation of neural progenitor cells, and impaired the survival and altered the fate decision of newly generated cells in the hippocampus. The expression levels of caspase-3 and glial fibrillary acidic protein were significantly higher in paraquat-treated mice than in control mice. Interestingly, paraquat reduced the phosphorylation of Akt, but did not affect the total amount of Akt. Conclusion In conclusion, our findings suggest that paraquat negatively affected adult hippocampal neurogenesis and cognition function.

关键词： paraquat learning memory neurogenesis phosphorylation

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防风式枸杞植保机的研制与试验

农机化研究 2021年第1期43卷 119-124,145页

作者：胡新德张东峰何军胡超白发宁夏大学机械工程学院银川750021 宁夏农林科学院枸杞工程技术研究所银川750002

针对目前枸杞植保作业中存在飘移严重、喷雾质量差、作业效率低等问题,设计了一款枸杞专用植保机,采用防风翼、智能喷雾控制系统、风送辅助系统、调节机构及大容量药箱等解决枸杞植保中存在的问题。试验结果表明:机器防风性能、喷雾质... 详细信息

针对目前枸杞植保作业中存在飘移严重、喷雾质量差、作业效率低等问题,设计了一款枸杞专用植保机,采用防风翼、智能喷雾控制系统、风送辅助系统、调节机构及大容量药箱等解决枸杞植保中存在的问题。试验结果表明:机器防风性能、喷雾质量、作业效率都符合设计要求,具有一定的推广价值。

关键词：防风植保机飘移升降伸缩智能喷雾性能试验

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葡萄厩肥施肥机的设计与试验

农机化研究 2020年第12期42卷 180-183,264页

作者：曾繁伟张东峰杨术明胡超胡新德白发宁夏大学机械工程学院银川750021

为推进宁夏地区葡萄产业机械化进程,针对葡萄施肥过程中机械存在的施肥深度不足、开沟施肥不能一体、排肥量小及肥料箱容量小等问题,研发了一种适用于宁夏地区葡萄种植的葡萄厩肥施肥机。通过对葡萄种植模式及施肥农艺要求的调研分析,... 详细信息

为推进宁夏地区葡萄产业机械化进程,针对葡萄施肥过程中机械存在的施肥深度不足、开沟施肥不能一体、排肥量小及肥料箱容量小等问题,研发了一种适用于宁夏地区葡萄种植的葡萄厩肥施肥机。通过对葡萄种植模式及施肥农艺要求的调研分析,确定施肥机的功能需求,进而确定了其总体设计方案。施肥机以轮式拖拉机为动力,采用牵引式挂接,大容量肥料箱内的输肥绞龙在拖拉机动力输出轴的驱动下强制搅拌排肥以防止厩肥架空,实现大排肥量的功能。同时,在肥料箱支撑轮后置立式螺旋开沟器,实现开沟施肥一体化作业。

关键词：葡萄厩肥施肥机开沟施肥一体

PCT、hs-CRP和血小板在新生儿败血症诊断中的价值及与病情的关系

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中国现代医生 2012年第27期50卷 56-57页

作者：胡新德刘宝涛湖南省邵阳市第二人民医院检验科湖南邵阳422000 湖南省邵阳市第二人民医院新生儿科湖南邵阳422000

目的探讨血清降钙素原(PCT)、超敏C-反应蛋白(hs-CRP)和血小板在新生儿败血症诊断中的价值及与病情的关系。方法对我院住院治疗的66例新生儿败血症患者临床资料进行回顾性分析,选择同期60例健康新生儿为对照组。比较两组血清PCT、hs-CR... 详细信息

目的探讨血清降钙素原(PCT)、超敏C-反应蛋白(hs-CRP)和血小板在新生儿败血症诊断中的价值及与病情的关系。方法对我院住院治疗的66例新生儿败血症患者临床资料进行回顾性分析,选择同期60例健康新生儿为对照组。比较两组血清PCT、hs-CRP和血小板的水平,计算三者诊断新生儿败血症的敏感性、特异性。结果败血症组患儿PCT及hs-CRP水平显著升高(P<0.05),血小板水平显著下降(P<0.05);PCT、hs-CRP和血小板诊断新生儿败血症的敏感性分别为84.8%、69.7%和53.0%,特异性为75.0%、50.0%和22.5%;败血症组患儿恢复期PCT及hs-CRP水平显著降低,血小板水平显著升高,与疾病初期相比差异具有统计学意义(P<0.05)。结论 PCT、hs-CRP和血小板对新生儿败血症具有诊断意义,能较好地反应患儿病情变化。

关键词：败血症新生儿降钙素原超敏C-反应蛋白血小板

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外阴白斑症(外阴营养不良)

湖南医学院学报 1982年第2期 173-177页

作者：胡信德王宝琼谢予萍孙玉美林丛湖南医学院第一附属医院妇产科湖南医学院病理解剖教研组

本文分析了临床诊断为“外阴白斑症”的200例病理切片,认为除少数伴有不典型增生者,可视为癌前病变外,外阴白斑症并非癌前病变。作者建议暂用“外阴白斑症(外阴营养不良)”作为临床和病理的诊断名称。200例患者经采用中西医结合的方法... 详细信息

本文分析了临床诊断为“外阴白斑症”的200例病理切片,认为除少数伴有不典型增生者,可视为癌前病变外,外阴白斑症并非癌前病变。作者建议暂用“外阴白斑症(外阴营养不良)”作为临床和病理的诊断名称。200例患者经采用中西医结合的方法进行治疗,有效率达99.48%。本文还报道了8例小儿外阴白斑症。

关键词：外阴白斑症小儿病理切片诊断名称癌旁外阴营养不良外阴白色病损不典型增生异型增生癌前病变外阴皮肤外阴癌大小阴唇类病变

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2079例新生儿核型分析初步报道

湖南医学院学报 1981年第3期 189-192页

作者：夏家辉李麓芸戴和平胡世泉何小轩许发明许嘉肖广惠胡信德卢惠霖湖南医学院医学遗传研究室湖南医学院附一院妇产科

新生儿染色体异常发生率的调查,国外已有八个用常规染色体技术、一个用 G 显带技术的调查资料。国内尚无报告。我们自1979年5月3日开始,用染色体 G 式显带技术对长沙市北区三个医院连续出生的新生儿脐血标本作了染色体检查,以探讨中国... 详细信息

新生儿染色体异常发生率的调查,国外已有八个用常规染色体技术、一个用 G 显带技术的调查资料。国内尚无报告。我们自1979年5月3日开始,用染色体 G 式显带技术对长沙市北区三个医院连续出生的新生儿脐血标本作了染色体检查,以探讨中国人群中染色体异常的发生率,至1981年5月19日止共完成2079人的检查,已肯定的染色体异常共15例。他们的核型见表1

关键词：染色体异常发生率核型照片显带技术脐血

取代乙烯顺反异构体键能和稳定性的密度泛函理论研究

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有机化学 2002年第3期22卷 200-205页

作者：胡宗球祝心德华中师范大学化学系武汉430079

对十四种取代乙烯 (XX′CCYY′)顺反异构体进行了DFT B3LYP/ 6 31G(d)水平的几何全优化的总能量、总键能的计算 .结果表明 ,对无共轭大π键的二取代乙烯 ,其顺式异构体的总能量比反式异构体的低 ,前沿轨道能隙、总键能和总π键能均比... 详细信息

对十四种取代乙烯 (XX′CCYY′)顺反异构体进行了DFT B3LYP/ 6 31G(d)水平的几何全优化的总能量、总键能的计算 .结果表明 ,对无共轭大π键的二取代乙烯 ,其顺式异构体的总能量比反式异构体的低 ,前沿轨道能隙、总键能和总π键能均比反式异构体的大 ,而对有共轭大π键的体系 ,其情况则刚好相反 .表明对于前者其顺式异构体比反式异构体稳定。

关键词：密度泛函理论取代乙烯顺反异构体键能稳定性 DFT 量子化学