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Cellular & Molecular Immunology 2017年第12期14卷 1023-1025页

作者： Xilong Kang Zhiming Pan xinan jiao Jiangsu Key Laboratory of Zoonosis YangzhouUniversityYangzhouJiangsu 225009China Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhouJiangsu 225009China Key Laboratory of Prevention and Control of Biological Hazard Factors(Animal Origin)for Agrifood Safety and Quality MOAYangzhou UniversityYangzhouJiangsu 225009China Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education YangzhouJiangsu 225009China

Flagellin,the main component of bacterial flagella,is a well-known pathogen-associated molecular pattern(PAMP)that can be recognized by the cell surface-localized Toll-like receptor 5(TLR5)and the cytosolic NOD-like receptor protein 4(NLRC4)inflammasome receptor NAIP5/6.1 Hosts can detect flagellated bacteria using the dual sensors,TLR5 and NAIP5/6,to recognize flagellin,and these sensors then initiate innate immune responses to protect the host from bacterial infection.Recognition by extracellular TLR5 can trigger the MyD88-dependent signaling pathway and activate nuclear factorκB,leading to the upregulation of several proinflammatory cytokines.

关键词： effect TLR5 initiate

Amino acids 89-96 of Salmonella typhimunum Tlagell=n represent the major domain responsible for TLR5-independent adjuvanticity in the humoral immune response

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Cellular & Molecular Immunology 2015年第5期12卷 625-632页

作者： Lei Zhang Zhiming Pan Xilong Kang Yun Yang Heekap Kang Na Zhang James M Rosati xinan jiao Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis Yangzhou China Jiangsu Key Laboratory of Zoonosis Yangzhou University Yangzhou China Ministry of Education Key Lab for Avian Preventive Medicine Yangzhou University Yangzhou China Division of Nephrology and Hypertension Department of Medicine Northwestern University Feinberg School of Medicine Chicago IL USA

Toll-like receptor 5 （TLR5） signaling in response to flagellin is dispensable for inducing humoral immunity, but alterations of aa 89-96, the TLR5 binding site, significantly reduced the adjuvanticity of flagellin. These observations indicate that the underlying mechanism remains incompletely understood. Here, we found that the native form of Salmonella typhimurium aa 89-96-mutant flagellin extracted from flagella retains some TLR5 recognition activity, indicating that aa 89-96 is the primary, but not the only site that imparts TLR5 activity. Additionally, this mutation impaired the production of IL-lp and IL-18. Using TLR5KO mice, we found that aa 89-96 is critical for the humoral adjuvant effect, but this effect was independent of TLR5 activation triggered by this region of flageUin. In summary, our findings suggest that aa 89-96 of flagellin is not only the crucial site responsible for TLR5 recognition, but is also important for humoral immune adjuvanticity through a TLR5-independent pathway.

关键词： adjuvanticity amino acids 89-96 Salmonella typhimurium flagellin TLR5 activity

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沙门菌调控NOD样受体机制的研究进展

中国兽医学报 2024年第2期44卷 396-402页

作者：许辰孟闯焦新安潘志明扬州大学江苏省人兽共患病学重点实验室江苏扬州225009 扬州大学农业农村部农产品质量安全生物性危害因子(动物源)控制重点实验室江苏扬州225009

沙门菌作为一种重要食源性病原菌,可广泛感染动物和人,严重威胁宿主健康。沙门菌入侵时,首先与宿主天然吞噬细胞等的模式识别受体(pattern recognition receptor,PRR)结合并激活天然免疫应答。NOD样受体(NOD-like receptors,NLR)作为一... 详细信息

沙门菌作为一种重要食源性病原菌,可广泛感染动物和人,严重威胁宿主健康。沙门菌入侵时,首先与宿主天然吞噬细胞等的模式识别受体(pattern recognition receptor,PRR)结合并激活天然免疫应答。NOD样受体(NOD-like receptors,NLR)作为一种细胞质PRR,在病原体感染和肿瘤免疫中发挥重要作用。NLR可被沙门菌鞭毛、分泌蛋白、LPS等多种毒力因子激活,组装炎性小体促进细胞分泌多种细胞因子抵御感染。而沙门菌也进化出多种机制如修饰鞭毛、细胞内形成带菌囊泡(Salmonella-containing vacuole,SCV)和分泌蛋白等阻止受体识别从而实现免疫逃逸,在宿主体内长期存活。现总结了近年来沙门菌激活NLR,以及沙门菌躲避NLR识别的相关研究进展,为进一步了解宿主应对沙门菌的天然免疫应答及疫苗开发提供思路。

关键词：沙门菌 NOD样受体炎症小体天然免疫免疫逃逸

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新农科人才培养模式探索

扬州大学学报（高教研究版） 2024年第1期28卷 24-32页

作者：陈祥梁昌镛焦新安扬州大学江苏扬州225009

根据新农科人才知识宽度、能力宽广和素质宽厚的要求,以及下得去农村、用得上专业技能、耐得住寂寞、上得来求学深造的培养目标,新农科人才培养应以优化组合、转型化合为总纲领,并形成跨界创新、跨学科专业建设的人才培养理念;应从思想... 详细信息

根据新农科人才知识宽度、能力宽广和素质宽厚的要求,以及下得去农村、用得上专业技能、耐得住寂寞、上得来求学深造的培养目标,新农科人才培养应以优化组合、转型化合为总纲领,并形成跨界创新、跨学科专业建设的人才培养理念;应从思想政治与专业教育、学科集群、教学团队、多元平台、课程体系、数字教育与传统教育、交流渠道、先进评价体系等八个方面的融合出发,构建新农科人才培养模式。

关键词：新农科地方综合性大学人才培养

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鸡白细胞介素-2原核表达及其活性鉴定

中国家禽 2024年第5期46卷 56-61页

作者：韩顺子黄霞孟闯耿士忠康喜龙潘志明焦新安扬州大学江苏省人兽共患病学重点实验室江苏扬州225009 扬州大学农业农村部农产品质量安全生物性危害因子(动物源)控制重点实验室江苏扬州225009 江苏高校动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009

试验旨在获得具有生物活性的鸡白细胞介素-2(ChIL-2)重组蛋白。试验以克隆获得的ChIL-2基因为模板,分别构建原核重组表达菌BL21(pCold-ChIL-2)和BL21(pGEX-6P-1-ChIL-2),进行诱导表达和纯化;通过BrdU ELISA法检测重组蛋白诱导鸡脾脏淋... 详细信息

试验旨在获得具有生物活性的鸡白细胞介素-2(ChIL-2)重组蛋白。试验以克隆获得的ChIL-2基因为模板,分别构建原核重组表达菌BL21(pCold-ChIL-2)和BL21(pGEX-6P-1-ChIL-2),进行诱导表达和纯化;通过BrdU ELISA法检测重组蛋白诱导鸡脾脏淋巴细胞的增殖情况,评估其生物活性;以rHis-ChIL-2免疫小鼠制备多克隆抗体。结果显示:成功构建了原核重组表达菌BL21(pCold-ChIL-2)、BL21(pGEX-6P-1-ChIL-2);经表达纯化后分别获得纯度较高的rHis-ChIL-2和rGST-ChIL-2蛋白,大小分别为14 ku和39.4 ku;rHis-ChIL-2和rGST-ChIL-2蛋白分别能够促进脾脏淋巴细胞显著或极显著增殖(P<0.01或P<0.001),均具有良好的生物活性;以纯化的rHis-ChIL-2免疫小鼠制备的多克隆抗体效价为5.12×105,可识别rHis-ChIL-2和rGST-ChIL-2,与真核表达的ChIL-2蛋白反应。结果表明,原核表达的重组蛋白ChIL-2具有良好的生物活性,采用其制备的多克隆抗体可识别真核表达的ChIL-2蛋白,为后期ChIL-2的检测奠定基础。

关键词： ChIL-2 原核表达生物活性多克隆抗体

Effect of endometrial thickness on the risk of ectopic pregnancy for frozen embryo transfer

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生殖与发育医学(英文) 2024年第2期8卷 75-81页

作者： Huang Li-Juan Zheng Qu Wang Xue-jiao Zhong Zhao-Hui Fu Li-Juan Lv Xing-Yu Geng Li-Hong Tang Xiao-Jun Ding Yu-Bin Wan Qi Department of Obstetrics and Gynecology Women and Children’s Hospital of Chongqing Medical University Chongqing China School of Public Health Joint International Research Laboratory of Reproduction and Development of the Ministry of Education of China Chongqing China The Reproductive Center Chengdu Xinan Gynecology Hospital Chengdu China School of Public Health Research Center for Medical and Social Development Chongqing Medical University Chongqing China Department of Pharmacology Academician Workstation Changsha Medical University Changsha China Department of Obstetrics and Gynecology West China Second Hospital Sichuan University Chengdu China

Objective: Few studies have explored the suitability of endometrial thickness (EMT) as a predictor of ectopic pregnancy (EP) in frozen embryo transfer (FET) cycles. Our study aimed to investigate the association between EMT and EP and explore the cutoff value of EMT to predict EP.Methods: This retrospective cohort study included women aged 20-45 years undergoing FET between March 2015 and September 2021. The primary outcomes and measured variables were EP and EMT, respectively. The threshold effect of EMT on EP was calculated based on a constructed smooth curve fitting. The patients were divided into two groups based on the threshold for comparison. A subgroup analysis was conducted to examine whether the relationship between EMT and EP remained consistent across the different subgroups. Additionally, a generalized linear model was used to analyze the interactions between the treatment factors and EMT in EP.Results: In total, 27,549 FET cycles were included, with 27,086 intrauterine and 463 ectopic pregnancies. The risk of EP in the EMT ≤8.7 mm group was 3.36 times higher than that in the EMT >8.7 mm group (odds ratio [OR]: 3.36, 95% confidence interval [CI]: 2.57-4.05). Subgroup analysis further indicated that a thin EMT was a risk factor for EP, independent of other risks. We also noted that diminished ovarian reserve, tubal factor infertility, transfer of two embryos, or transfer of cleavage-stage embryos may further enhance the risk of EP in patients with thin endometrium.Conclusions: Thin endometrium was associated with an increased risk of EP. Patients with EMT less than 8.7 mm need close medical observation and management after embryo transfer. In addition, diminished ovarian reserve, tubal factor infertility, transfer of two embryos, or transfer of cleavage-stage embryos may significantly enhance the effect of a thin endometrium on the risk of EP.

关键词： Endometrial thickness Thin endometrium Ectopic pregnancy FET cycle Risk factors

Evaluation of immunogenicity and protective efficacy elicited by Mycobacterium bovis BCG overexpressing Ag85A protein against Mycobacterium tuberculosis aerosol infection

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Evaluation of immunogenicity and protective efficacy elicite...

Mycobacterium tuberculosis Beijing Genotype Family Strain Isolated from Naturally Infected plateau zokor(Myospalax baileyi) in China

中国畜牧兽医学会动物传染病学分会第九次全国会员代表大会...

作者：徐正中 Xiang Chen Ting Hu Chuang Meng xinan jiao Jiangsu Key Laboratory of Zoonosis Yangzhou University

Introduction Tuberculosis(TB)is the number one infectious disease killer in the world,it is responsible for about 1.5 million deaths and an estimated 9.6 million new cases in 2014.The bacillus Calmet te-Guerin(BCG)vaccine is effective against miliary and meningeal TB in children,but provides variable protection against pulmonary TB in adults.As a consequence,alternative vaccines or supp

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Mycobacterium tuberculosis Beijing Genotype Family Strain Is...

N-Ac-PGP通过激活TLR4诱导巨噬细胞的M1极化并提高慢性阻塞性肺疾病炎症水平

中国畜牧兽医学会动物传染病学分会第九次全国会员代表大会...

作者：孙林 Xiang Chen Wenhai Zhang Gencheng Huang Yuanyuan Zhang Zhengzhong Xu Baofa Yin Wanhong Wei xinan jiao Kanglin Wan Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou University State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention Department of Bioscience College of Bioscience and BiotechnologyYangzhou University

Mycobacterium tuberculosis(MTB) remains one of the world’s most successful pathogens,inf ecting one-third of humans.Although rodents are rarely clinically affected by mycobacteriosis,they can serve as potential reservoirs of MTB for wildlife,domestic animals and humans.Here,we in vestigated the prevalence of Mycobacteria in the wild plateau zokor(Myospalax baileyi),a rodent species endemic in China.In May 2010,a total of nine wild plateau zokors with no clinical signs of mycobacteriosis w ere caught in the Haibei Alpine Meadow Ecosystem Research Station,Northwest Institute of Plate au Biology,Chinese Academy of Sciences,Qinghai province,China.After dissection,the lungs wer e removed,homogenized and decontaminated,and then the treated homogenate samples were inocu lated for Mycobacteria isolation.Finally,three isolates,namely,JSA11006,JSA11010 and JSA11028,were successfully cultured.JSA11006 was identified as Mycobacterium setense,and JSA11028 was identified as Mycobacterium septicum.JSA11010 was categorized as MTB,belonging to the Beijing genotype family with Spoligo International Type number = 1,and its variable number of t andem repeats profile was 2-3-4-3-4-3-3-4-3-7-4-4-4-2-5-1-5-3-3-4-4-6-3-3,as defined by the numbe r of tandem repeats in MIRU02,Mtub04,ETRC,MIRU04,MERU40,MIRU10,MIRU16,Mtub21,MIRU20,QUB11 b,ETRA,Mtub29,Mtub30,ETRB,MIRU23,MIRU24,MIRU26,MIRU27,Mtu b34,MIRU31,Mtub39,QUB26,QUB4156 and MIRU39.To the best of our knowledge,this is the first report of an MTB infection in plateau zokor,and this species may be an important reservoir for MTB and a zoonotic threat to humans and oth er animals.

关键词： Mycobacterium tuberculosis Beijing genotype plateau zokor Myospalax baileyi

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中国病理生理杂志 2023年第11期39卷 2060-2066页

作者：刘倩杨姣石西南徐悦杜晓华昆明医科大学第一附属医院云南昆明650032 云南中医药大学云南昆明650500

目的:探究N-乙酰化脯氨酸-甘氨酸-脯氨酸(N-acetylated proline-glycine-proline,N-Ac-PGP)通过Toll样受体4(Toll-like receptor 4,TLR4)诱导巨噬细胞M1极化对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)炎症反应的影响。方法:检测COPD患者痰液中N-Ac-PGP和M1型巨噬细胞炎性细胞因子的表达。体外培养人单核细胞白血病细胞THP-1,使用佛波脂(phorbol 12-myristate 13-acetate,PMA)诱导使之分化为M0型巨噬细胞,然后使用脂多糖(lipopolysaccharide,LPS)和N-Ac-PGP诱导M0型巨噬细胞极化为M1型。采用RTqPCR和Western blot法分别检测巨噬细胞表面标志物CD11b、CD45、CD86和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)的mRNA和蛋白表达水平。使用ELISA检测M1型巨噬细胞炎性细胞因子肿瘤坏死因子α(tu-mor necrosis factor-α,TNF-α)、白细胞介素1β(interleukin-1β,IL-1β)、IL-6和IL-23的水平。最后抑制TLR4通路,再次检测巨噬细胞的极化情况。结果:N-Ac-PGP与M1型巨噬细胞炎性细胞因子表达量呈显著正相关。PMA处理THP-1细胞成功诱导分化为M0型巨噬细胞。LPS和N-Ac-PGP处理后M1型巨噬细胞的标志蛋白表达显著增加(P<0.05),证明经过LPS和N-Ac-PGP处理的M0型巨噬细胞向M1型极化。同时,炎性细胞因子TNF-α、IL-1β、IL-6和IL-23的表达上调(P<0.05)。进一步的实验中,TLR4抑制剂与N-Ac-PGP联用逆转了N-Ac-PGP对M0型巨噬细胞向M1型极化的促进作用,也显著降低了炎性细胞因子的表达水平(P<0.05)。结论:N-Ac-PGP通过激活TLR4诱导的巨噬细胞M1极化促进COPD炎症反应。

关键词：慢性阻塞性肺疾病 N-乙酰化脯氨酸-甘氨酸-脯氨酸 Toll样受体4 巨噬细胞极化炎症

基于沙门氏菌PagN外膜蛋白的广谱单克隆抗体的制备与鉴定

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中国家禽 2023年第4期45卷 69-75页

作者：孟闯丁睿清尚月月刘钧徐双媛康喜龙焦新安潘志明扬州大学江苏省人兽共患病学重点实验室江苏扬州225009 江苏高校动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009 扬州大学农业农村部农产品质量安全生物性危害因子(动物源)控制重点实验室江苏扬州225009

为制备针对沙门氏菌特异性外膜蛋白PagN的单克隆抗体(MAb),研究通过构建重组质粒pColdⅠ-pagN和pGEX-6P-1-pagN,利用大肠杆菌系统表达并纯化了肠炎沙门氏菌His-PagN和GST-PagN重组蛋白,免疫6~8周龄BALB/c小鼠,利用B淋巴细胞杂交瘤技术... 详细信息

为制备针对沙门氏菌特异性外膜蛋白PagN的单克隆抗体(MAb),研究通过构建重组质粒pColdⅠ-pagN和pGEX-6P-1-pagN,利用大肠杆菌系统表达并纯化了肠炎沙门氏菌His-PagN和GST-PagN重组蛋白,免疫6~8周龄BALB/c小鼠,利用B淋巴细胞杂交瘤技术筛选获得2株能稳定分泌PagN蛋白MAb的杂交瘤细胞株3B3和3F10。结果显示:3B3和3F10的亚型均为IgG2b,ELISA效价分别为1.14×10^(9)和1.32×10^(8),Westernblot分析显示其均能特异性结合His-PagN蛋白;进一步分析显示3B3和3F10可特异性识别9种不同血清型沙门氏菌,而不与9种非沙门氏菌菌株反应;全菌包板间接ELISA结果显示3F10能特异性识别不同血清型沙门氏菌,显示出用于沙门氏菌检测的潜能。研究表明制备的单克隆抗体3B3和3F10可特异性识别不同血清型沙门氏菌PagN蛋白,为沙门氏菌检测提供了重要生物材料。

关键词：沙门氏菌 PagN 外膜蛋白单克隆抗体反应谱